News from Jennifer Doudna's lab at
@UCBerkeley
@igisci
. Tweets from lab members and not Jennifer Doudna unless signed JD. Tweets represent personal views only.
I am excited and a bit overwhelmed with emotion at the news of the approval of CASGEVY in the UK. Going from the lab to an approved
#CRISPR
therapy in just 11 years is a truly remarkable achievement. (1/3)
A decade after we showed CRISPR systems could be used for programmable genome editing — our latest review is a look back at the work that has advanced this technology, and a look forward to the future of what may still be yet to come! 🧬✂️
Brace yourself… More robust than RNAi and less toxic than Cas13… check out our latest story on CRISPR-Csm for precise RNA targeting!!
@NatureBiotech
@igisci
We’re excited to announce the soft-launch of a new
#CRISPR
database, CasPEDIA (), a product of a large team co-led by
@ben_a_adler
and Marena Trinidad!
CasPEDIA summarizes and contextualizes enzymatic properties of widely used Cas enzymes:
1/
Looking for the ideal CRISPR-Cas system for your experiment? CasPEDIA, our wiki of Cas nucleases, introduces a new functional nomenclature (CasID) to help you get started!
Introducing CRISPRpedia – our new, free online textbook for everything CRISPR! Learn (& teach) about
#CRISPR
from its role in nature to development of the genome editing tool, to applications in research, medicine & agriculture. Check it out!
#STEMEd
I am especially pleased that the first
#CRISPR
therapy helps patients with sickle cell disease, a disease that has long been neglected by the medical establishment. This is a win for medicine and for health equity. (2/3)
Brace yourself… More robust than RNAi and less toxic than Cas13… check out our latest story on CRISPR-Csm for precise RNA targeting!!
@NatureBiotech
@igisci
A few days of much deserved time off during our lab retreat! We talked some science, drank plenty of wine, and explored beautiful Sonoma via hot air balloon, bike, safari Jeep, and horseback.
Super proud to have played a small role in this work - a true multi-kingdom tale.
Side bar: watching
@PeterHYoon
make worms glow *with viruses* was a major highlight of 2022 for me.
New preprint from the lab: how RNA-guided nucleases🐛wormed 🪱 their way into eukaryotes. Proud of this collaborative effort and analysis led by
@PeterHYoon
,
@PetrSkopintsev
, and
@honglue1
!
Please welcome baby boy Jude, born 12/30! A big thank you to all of the wonderful providers
@UCSF
who helped us through fertility, pregnancy, and newborn feeding and fussiness. Immensely grateful to experience the amazing care of my home department
@UCSFChildrens
@UCSFPediatrics
Beautiful work from graduate student Carolyn Huang as well as
@ben_a_adler
characterizing, investigating, and harnessing the function of enigmatic Cas enzyme, Cas12c! Check out our new paper in
@MolecularCell
investigating nature's CRISPRi
@igisci
@berkeleyMCB
@QB3
Huge congrats to longtime collaborator
@BanfieldJill
!!! In good company with other microbiologists like Louis Pasteur, and the first woman to win the Leeuwenhoek Medal. So very well deserved🥂
@igisci
@UCBerkeley
Celebrating that Jill receives the Leeuwenhoek Medal this year! It is is granted every ten years to the scientist judged to have made the most significant contribution to microbiology during the preceding decade. Cheers
@BanfieldJill
!
New paper on engineered Cas12a variants! Through both structure guided engineering and directed evolution we minimized trans activity, increased genome editing in human cells, and enhanced HDR efficiencies
@igisci
Check out our latest preprint on CRISPR integration!! We found a natural protein fusion that coordinates spacer selection, processing, and integration as the first step of adaptive immunity
@igisci
@UCB_Chemistry
How do CRISPR immune systems make molecular memories? Joy, Petr and I characterized an all-in-one trimmer-integrase to ask questions about CRISPR adaptation. Really excited to share this one - my first preprint
@doudna_lab
@igisci
. Check it out!
New preprint from the lab co-led by
@AmyReginaEggers
and
@KaiChen_1990
!
iGeoCas9’s improved ability to rapidly unwind DNA is driven by 3 mutations. These mutations can be engineered into other Cas9’s to boost gene editing abilities! Check out
@AmyReginaEggers
post for details!
Beautiful new review from the lab, written by soon-to-be lab alum Joy Wang and lab alum Patrick Pausch! Check it out if you want to learn about structural biology in all aspects of CRISPR
Hey
#CRISPR
friends! ✂️🧬 Joy Wang, myself and Jennifer
@Doudna_lab
wrote a
@NatureRevMicro
review on the ‘Structural biology of CRISPR-Cas immunity and genome editing enzymes’. (1/4)
Excited to share this pre-print on recent developments in non-viral delivery of CRISPR-Cas9 to the mouse brain, following up on work from the
@doudna_lab
published in 2017
Out now – inspiring work from
@ben_a_adler
on how to edit the most abundant organisms on earth: bacteriophages. A tale of how one of the rarest CRISPR systems can tackle phage diversity and is a robust phage-editing tool.
More editing the Phage Genomic Universe coming soon!
Super excited to share my first publication with
@doudna_lab
@NatureMicrobiol
: RNA-targeting CRISPR-Cas13 Provides Broad-spectrum Phage Immunity
Now, with even more phage genome editing (and of course, CRISPR-Cas13)
1/
New pre-print led by Post-doc in the lab
@JRo_Hamilton
. Tackling one of the biggest challenges in therapeutic genome editing - cell specific delivery - with programmable enveloped delivery vehicles!
🧬🚨 New preprint from
@ben_a_adler
and Muntathar Al-Shimary in co-led collaboration with
@bradyfcress
lab!
Catalytically deactivated Cas13 can inhibit diverse phages by mere RNA binding, enabling CRISPR interference in these fascinating viruses!
I’m really excited to share our new pre-print “Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi”, with co-first author Muntathar Al-Shimary
@doudna_lab
and the newly-minted
@bradyfcress
lab!
🧵🧵
1/
Join the team developing the new field of
#microbiome
editing! We are recruiting a jointly advised postdoc with Jennifer Doudna
@doudna_lab
to apply new CRISPR-Cas systems to editing clinically and environmentally relevant microbiomes.
Learn more ⬇️
BiomeForge, a new microbiome editing initiative in UC Berkeley's
@igisci
, is
#hiring
! The postdocs will create efficient microbiome editing tools using various
#CRISPR
-Cas systems, engineered phages, and next-gen control strategies.
New preprint 🚨 from
@connortsuchida
in our lab. We utilized multiple CRISPR screens to investigate Cas9-induced chromosome loss. Surprisingly, we found a clinical methodology to mitigate this cellular fate. Great collaboration with
@yimmieg
@HowardYChang
@carlhjune
@j_eyquem
Excited to share our latest story from
@doudna_lab
systematically studying the phenomenon and mitigation of Cas9-induced chromosome loss in clinical human T cells now on
@biorxivpreprint
A special day for our fearless leader. Congratulations to both Eva,
@CramerLab
, and all of the lab members who contributed toward our understanding of the molecular mechanisms of transcription initiation.