Nature - Structural studies on Scytonema hofmanni CRISPR-associated transposon protein complexes indicate a mechanism for RNA-guided DNA transposition involving Cas12k, TnsC and TnsB.

Nature - Cryo-electron microscopy structures of Streptococcus pyogenes Cas9 in multiple DNA-bound states provide insights on the mechanism of Cas9 activation by target DNA.

Cryo-EM structure of a type V CRISPR-associated transposon recruitment complex reveals the mechanism linking the RNA-guided pseudonuclease Cas12k and the transposition machinery, and the involvement...

CRISPR systems have been co-opted by Tn7-like elements to direct RNA-guided transposition. Type V-K CRISPR-associated transposons rely on the concerted activities of the pseudonuclease Cas12k, the...

The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is determined by complementarity to a 20-nucleotide segment in its guide RNA. However, Cas9 can bind and cleave...

Type III CRISPR-Cas systems provide adaptive immunity against foreign mobile genetic elements through RNA-guided interference. Sequence-specific recognition of RNA targets by the type III effector...

Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA cleavage[1][1]-[3][2]. The programmable activity of Cas9 has been widely utilized for genome editing applications[4...

The detection of molecular patterns associated with invading pathogens is a hallmark of innate immune systems. Prokaryotes deploy sophisticated host-defense mechanisms in innate anti-phage immunity....

Biochemical and structural investigations of the human tRNA ligase complex reveal the details of its molecular assembly and provide insights into the reaction mechanism of its catalytic subunit.

RtcB enzymes are RNA ligases that play essential roles in tRNA splicing, unfolded protein response, and RNA repair. In metazoa, RtcB functions as part of a five-subunit tRNA ligase complex (tRNA-LC)...