Sam Sternberg @shsternberg Twitter profile | Pikagi

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Sam Sternberg

@shsternberg

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Assistant professor at Columbia University / CRISPR aficionado / Protein–RNA biochemist / Co-author with Jennifer Doudna of "A Crack In Creation"

New York, NY

https://t.co/7D3g8oxW8J

Joined April 2015

Don't wanna be here? Send us removal request.

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@shsternberg

Sam Sternberg

5 months

Ever wonder if nature already 'invented' RNA-guided transcription factors like CRISPRi/a? Then look no further... we found many examples of domesticated, nuclease-dead TnpBs that use guide RNAs to repress genes! In bacteria AND phage! A wonderful team effort led by Tanner Wiegand

@SternbergLab

Sternberg Lab

5 months

New pre-print from the Sternberg Lab! Transposon-encoded nucleases (TnpB/IscB) gave rise to the CRISPR-associated proteins (Cas9 and Cas12), but were TnpB-like proteins domesticated for other functions? 𝙔𝙀𝙎!!

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@shsternberg

Sam Sternberg

4 years

Nobels are also bittersweet, because SOOO many more folks deserve the award than can receive it. Michael ‘Michi’ Hauer was the first to purify Cas9 in Jennifer’s lab, whole working with Martin Jinek. He died last year, but would have been ecstatic to hear the news today.

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@shsternberg

Sam Sternberg

4 years

What I imagine Jennifer doing right now at home, 4:30 in the morning... 😜

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@shsternberg

Sam Sternberg

4 years

A lot of emotions this week. This award is thanks to an AMAZING group of students/postdocs/technicians in the lab, who make coming into lab every day a real treat. Can't wait for all the discoveries we'll continue to make together :)

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@ColumbiaPS

Columbia Vagelos College of Physicians & Surgeons

4 years

Congrats to Samuel Sternberg @shsternberg , one of 5 #ColumbiaVPS scientists to receive a 2020 grant from @NIHDirector 's High-Risk, High-Reward program. He is creating new methods to engineer the human genome. #NewInnovatorAward

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@shsternberg

Sam Sternberg

4 years

Zoom celebration of Jennifer's Nobel, with large D-Lab contingent joining in. As always, Jennifer crediting the many people that were so critical to the work in her lab.

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@shsternberg

Sam Sternberg

5 years

My lab's first paper came online today, the result of a thrilling project with my first three PhD students, led by the brilliant @SanneKlompe . See her *first* Twitter thread below to learn more... and follow her! (And also follow @sternberglab :)

@SanneKlompe

Sanne Klompe

5 years

Hey Twitter! I'm super stoked to plug the first paper from the Sternberg Lab! Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration (). Some takeaways from our paper: 1/8

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@shsternberg

Sam Sternberg

7 months

Thrilled to see our latest work published this week, on the essential roles of IscB/TnpB -- ancestral homologs of Cas9/Cas12 -- in transposon maintenance & spread. A wonderful team effort led by the talented Chance Meers! Thread on bioRxiv version below...

Transposon-encoded nucleases use guide RNAs to promote their selfish spread

Nature - TnpB and IscB nucleases use transposon-encoded guide RNAs to target genomic sequences for cleavage, thereby favouring copying and spreading of transposable elements.

@sanjana_pesari

Sanjana Pesari

@sanjana_pesari

1 year

Great news - our lab's latest manuscript is out on bioRxiv! Led by postdoc Chance Meers, we discovered that RNA-guided DNA cleavage by transposon-encoded nucleases arose to facilitate the pervasive spread and inheritance of transposons. 🧵: (1/6)

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@shsternberg

Sam Sternberg

4 years

Jennifer Doudna: amazing mentor, scientist, writer, etc. Also always down for a good party. Here we dragged her and @jhdcate to check out the @ForeverlandBand at Bimbo's 365 Club in SF. She's got some wicked dance moves!

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@shsternberg

Sam Sternberg

4 years

Our newest manuscript is live on bioRxiv! Beautiful work led by Leo @Vopinator in the lab, for high-efficiency, multiplexed DNA insertions in bacteria using CRISPR RNA-guided integrases. A great collaboration w/ @RondaCarlotta and @harriswangnyc . 1/n

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CRISPR RNA-guided integrases for high-efficiency and multiplexed bacterial genome engineering

Tn 7 -like transposons are pervasive mobile genetic elements in bacteria that mobilize using heteromeric transposase complexes comprising distinct targeting modules. We recently described a Tn 7...

www.biorxiv.org

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@shsternberg

Sam Sternberg

4 years

I'm really excited for this work to finally be published! Fantastic team effort from my colleagues @CaribouBio (especially Peter Cameron), @StanBrouns , and others. It's been fun seeing Cascade and Type I CRISPR systems get some time in the spotlight this year :)

@NatureBiotech

Nature Biotechnology

4 years

Harnessing type I CRISPR–Cas systems for genome engineering in human cells

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@shsternberg

Sam Sternberg

7 years

Thrilled to announce that I'll be starting my lab at Columbia University in 2018, as Asst Prof in Dept of Biochem & Molecular Biophysics 1/n

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@shsternberg

Sam Sternberg

1 year

I'm excited to share our recent work exploring the role of IscB/TnpB nucleases in transposition... which helps explain the mysterious evolutionary origins of RNA-guided DNA cleavage by Cas9 & Cas12! A great team effort led by Chance Meers. Read more in Sanjana's thread below!

@sanjana_pesari

Sanjana Pesari

@sanjana_pesari

1 year

Great news - our lab's latest manuscript is out on bioRxiv! Led by postdoc Chance Meers, we discovered that RNA-guided DNA cleavage by transposon-encoded nucleases arose to facilitate the pervasive spread and inheritance of transposons. 🧵: (1/6)

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@shsternberg

Sam Sternberg

4 years

Wowza, what news to wake up to!! Congrats to Jennifer and Emmanuelle, and the many others, who contributed to this breakthrough. Go CRISPR!!

@NobelPrize

The Nobel Prize

4 years

BREAKING NEWS: The 2020 #NobelPrize in Chemistry has been awarded to Emmanuelle Charpentier and Jennifer A. Doudna “for the development of a method for genome editing.”

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@shsternberg

Sam Sternberg

1 year

We were lucky to have Dr. Jennifer Doudna join our Precision Medicine course @Columbia yesterday as a special guest! The timing was fortuitous, as our topic was the potential of CRISPR technology to cure disease, alongside critical ethical and societal considerations. @igisci

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@shsternberg

Sam Sternberg

2 years

I'm thrilled to share our new study out today in @Nature on the role of TnsC in enhancing the fidelity of RNA-guided transposition. A wonderful collaboration with @IsraelF96135088 . For more info, check out lead author Florian's ( @fthoffmann ) thread below!

Hoffmann,Kim,Beh_2022_Nature.pdf

drive.google.com

@fthoffmann

Florian Hoffmann

2 years

Excited that our paper is now online @Nature ! Selective TnsC recruitment enhances the fidelity of RNA-guided transposition - the result of a fantastic collaboration of the @shsternberg and @IsraelF96135088 labs. () A thread: 1/10

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@shsternberg

Sam Sternberg

5 months

Exciting new work from the lab, on the marriage of transposons, CRISPR-like nucleases, guide RNAs, and self-splicing ribozymes! Check out the thread below from (Twitter-less) lead author, Rimante. A wonderful team effort

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Antagonistic conflict between transposon-encoded introns and guide RNAs

TnpB nucleases represent the evolutionary precursors to CRISPR-Cas12 and are widespread in all domains of life, presumably due to the critical roles they play in transposon proliferation. IS605-fam...

www.biorxiv.org

@SternbergLab

Sternberg Lab

6 months

We’re beyond excited to share the newest pre-print from the Sternberg Lab! You thought all bacterial transposons (aka IS elements) were simple? Think again…🤓1/6

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@shsternberg

Sam Sternberg

3 years

Massively proud of Leo's elegant & thorough study out in NBT, developing CRISPR-transposons for high-efficiency & high-fidelity DNA integration in diverse bacteria. Lots of exciting ideas on advancing this even further, together w/ collaborators @harriswangnyc and @RondaCarlotta

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@Vopinator

Leo Phuc Vo

3 years

My first author paper is out today @NatureBiotech ! We describe efficient and multiplexed engineering of bacteria using our CRISPR-transposon system INTEGRATE. Huge thanks to @shsternberg for his guidance, and to our amazing collaborators @RondaCarlotta and @harriswangnyc !

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@shsternberg

Sam Sternberg

2 years

Today we report the characterization of 18 new Type I-F RNA-guided transposases in @MolecularCell , with many showing high-efficiency & high-fidelity DNA integration. PDF link below, and great 🧵 from lead author @SanneKlompe

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@SanneKlompe

Sanne Klompe

2 years

So happy to share that we’re now online @MolecularCell ! Evolutionary and mechanistic diversity of Type I-F CRISPR-associated transposons (CASTs). Here are some takeaways from our paper (). 1/8

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@shsternberg

Sam Sternberg

7 months

We're hiring! Join the Biochemistry and Molecular Biophysics department here at Columbia, in wonderful NYC, and pursue your best scientific ideas amidst fantastic colleagues and a growing community. Happy to answer any questions about the department or application process.

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@LomvardasLab

LomvardasLab

7 months

Who would not want to start the independent career working with this amazing group of junior faculty? Having peers that get you, share your problems, and can have a drink with you at the end of the week is key for success! BMB is hiring- please apply:

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@shsternberg

Sam Sternberg

1 year

Really proud to share George & Rebeca's work applying CAST systems for targeted DNA integration in human cells, out today in NBT! I'm thankful for contributions made by all co-authors, and excited for more work that is still to come! Check out the🧵below

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@SternbergLab

Sternberg Lab

1 year

George & Rebeca here for the Sternberg Lab's inaugural tweet! We are excited to describe the reconstitution of CRISPR-associated transposases (CASTs) in human cells for programmable DSB-free insertion of large DNA cargo at genomic loci @NatureBiotech 🧬 1/8

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@shsternberg

Sam Sternberg

4 years

So proud of this heroic effort from Tyler in the lab, working together with the brilliant Israel Fernandez @IsraelF96135088 , in which they solved structures of TniQ-Cascade. Our first glimpses into the molecular basis for RNA-guided DNA integration by CRISPR-transposons. Kudos!!

@HalpinHealy

Tyler S Halpin-Healy

4 years

And we're live in Nature, Thanks again to @IsraelF96135088 and @shsternberg for the mentoring, and @SanneKlompe for the aid.

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@shsternberg

Sam Sternberg

5 years

Thrilled to post this preprint, the result of a really fun collaboration with @IsraelF96135088 led by the very talented Tyler @HalpinHealy ! Our first glimpses into the machinery that directs RNA-guided DNA insertion, and how CRISPR and transposition proteins work together!

@HalpinHealy

Tyler S Halpin-Healy

5 years

Hot off the press from the @SternbergLab Can't thank @SanneKlompe @shsternberg @IsraelF96135088 enough for superhuman effort.

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@shsternberg

Sam Sternberg

2 years

Lots of great vibes at @CSHL CRISPR meeting this week. What a thrill to see @SanneKlompe give a fantastic talk to kick off the meeting, followed by George & Rebeca (Twitterless) presenting their poster to a non-stop crowd for 3 hours. Volleyball match w/ friends capped the week

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@shsternberg

Sam Sternberg

5 years

Finally! Our plasmids for leveraging programmable integrases for high-specificity RNA-guided DNA integration in bacteria are now available at Addgene. Enjoy :)

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@shsternberg

Sam Sternberg

2 years

Thrilled to see @SanneKlompe recognized with this award from @RNASociety — very well deserved, for her discovery and exploration of CRISPR RNA-guided transposases!

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@RNASociety

The RNA Society

2 years

Congratulations to all the winners of our annual RNA Society awards!!!!

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@shsternberg

Sam Sternberg

6 months

Big update: we're hiring a new lab manager!! Come join a fantastic group of scientists in the @SternbergLab at Columbia University, in the heart of New York City, and contribute to cutting-edge CRISPR and genome engineering research. Please re-post and DM me with your interest

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@shsternberg

Sam Sternberg

5 years

The Sternberg Lab is recruiting postdocs! Our new paper is out, and we have more ideas and ongoing projects than we can possibly do ourselves. Come join us!! Biochem/biophys/genetics/structure/gene editing/... Please retweet!

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@shsternberg

Sam Sternberg

2 years

CRISPR 2022 conference was a blast, with lots of exciting new transposon biology! Fitting, to come just before #TransposonDay2022 . We didn’t catch everyone for the pic but some key players ⁦ @JoePetersLab ⁩ ⁦ @kellogg_liz ⁩ ⁦ @fthoffmann ⁩ ⁦ @MikePetassi ⁩ …

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@shsternberg

Sam Sternberg

9 months

What a day! We had such a blast sharing our science with middle-schoolers in Long Island, at the inaugural Cutting-Edge Science Camp! Big shout-out to all the volunteers from @Columbia , and to @SawickiScience @jameshmccabe @dharls1 @WenWanso and all the teachers from East Meadow!

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@shsternberg

Sam Sternberg

7 years

This response to CRISPR off-targets @naturemethods paper hits the nail on the head, IMO. From @geochurch et al.

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@shsternberg

Sam Sternberg

5 years

This breaking news story sure is an absolute bombshell for the #GeneEditSummit in Hong Kong this week. No doubt countless speakers are scrambling to update slides as we speak. All of a sudden the message is shifted from "should we" to "oh shit someone did it, now what?!"

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@shsternberg

Sam Sternberg

6 years

Yesterday was my first day @Columbia ! I'm incredibly stoked to be in such an inspiring environment and have the opportunity to work with amazing scientists here. Day 1 highlights: Landweber group meeting, orientation with my fantastic dept staff *stock photo (not from my office)

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@shsternberg

Sam Sternberg

7 years

In light of yesterday's news about gene-edited piglets, here's a handy refresher for how gene editing work (from legit scientific journal)

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@shsternberg

Sam Sternberg

10 months

Exciting new work on the molecular basis of "off-target" (➡️ untargeted) DNA integration events with type V-K CASTs, led by the talented @jerrintgeorge . Punchline: these transposases are more prone to CRISPR-independent transposition. See the 🧵 below...

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Mechanism of target site selection by type V-K CRISPR-associated transposases

Unlike canonical CRISPR-Cas systems that rely on RNA-guided nucleases for target cleavage, CRISPR-associated transposases (CASTs) repurpose nuclease-deficient CRISPR effectors to facilitate RNA-gui...

www.biorxiv.org

@jerrintgeorge

Jerrin Thomas George

10 months

We are super excited to share our work unraveling the mechanistic intricacies of target site selection by type V-K CRISPR-associated transposases now available as a preprint on bioRxiv!🧬 1/16

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@shsternberg

Sam Sternberg

2 years

Incredible line-up of talks to celebrate Joachim Frank’s 80+ birthday today @ColumbiaPS . Peter Moore just gave a captivating survey of ribosome work over the decades, starting with 1950s images of rough ER, to early crystal structures, to CryoEM. Crazy how far the field has come

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@shsternberg

Sam Sternberg

6 years

Many humbling firsts for me in the new job. Yesterday's faculty meeting, Richard Axel presenting, was great. ( @joachimfrank missed you there). And then just now, @rubenlgonzalez , my undergrad advisor, sitting in MY office 12 years after he gave me my first research opp

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@shsternberg

Sam Sternberg

6 years

Last time at UC Berkeley seeing Jennifer @doudna_lab and @jhdcate , before my move to Columbia. Bittersweet goodbye for sure, excited for the next step but will miss this place!

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@shsternberg

Sam Sternberg

4 years

A question for the community: Six months ago, @IsraelF96135088 posted our new cryoEM manuscript on @biorxivpreprint bioRxiv, which was a follow-up to the CRISPR-transposon discovery we published the month before. The cryoEM paper just came out in Nature last month. 1/n

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@shsternberg

Sam Sternberg

6 years

Whew! Good news – was worried that CRISPR–Cas9 might not work effectively in the swamp buffalo

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Sam Sternberg

7 years

Embryo editing is cool and all, but so is engineering hyper-accurate Cas9! Congrats @janiceschen @doudna_lab et al.

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Sam Sternberg

5 years

We had a blast geeking out on all things Tn7 / transposon / CRISPR with @JoePetersLab at Columbia over the last few days. Thanks for a fantastic talk and visit Joe, looking forward to many more discussions to come... :)

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Sam Sternberg

9 years

@TheStemCell @pknoepfler On the topic of pigs, here's my favorite #CRISPR -related figure ever! http://t.co/yFAtHIkslD http://t.co/jTwUK6lGdk

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@shsternberg

Sam Sternberg

1 year

Check out this thread from @mattwalkerhi on exciting new work from the lab, dissecting transposon end requirements of CASTs using pooled libraries and HTS. Very proud of the collaborative effort from Matt, Dr. @SanneKlompe , and Columbia undergrad @DennisJZhang1 ! @ColumbiaBiochem

@mattwalkerhi

Matt Walker

1 year

Thrilled to release our manuscript on biorxiv! @SanneKlompe and I investigated a CRISPR-associated transposon (CAST), uncovering a novel cellular factor and molecular requirements that will be critical for genome engineering. (1/7)

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@shsternberg

Sam Sternberg

5 years

This is freakin' mind-blowing... "Becoming" by Jan van IJken. A single cell become a complete organism in six pulsing minutes of timelapse

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@shsternberg

Sam Sternberg

4 years

Hey CRISPR peeps: what's your favorite algorithm/software for identifying potential protospacer matches from a list of spacers (or a CRISPR array)? I've used CRISPRTarget () in the past but am wondering if there's anything newer/better. Thanks for the help!

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@shsternberg

Sam Sternberg

6 years

Super cool! Watch Cas9 pop off DNA. I think the audible gasp at CRISPR 2017 meeting when Nureki played this video was one of largest reactions I've heard to new data being presented.

@CRISPRjournal

The CRISPR Journal

6 years

A video is worth <280 characters h/t and congratulations to @hnisimasu and colleagues

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@shsternberg

Sam Sternberg

6 years

Thrilled to see this paper from Zucai Suo's lab. It is often ignored/forgotten that Cas9 does NOT generate blunt DSBs, but can resect non-target strand extensively. Has importance for DNA repair outcomes in gene editing experiments

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@shsternberg

Sam Sternberg

5 years

Just got off phone with a colleague of mine, @EricFormanMD , who’s lab director of Columbia University Fertility Center @ColumbiaWomens . Learned quite a bit about standard practices (in the US at least) for the application of IVF with HIV discordant couples. Some take-homes... 1/n

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@shsternberg

Sam Sternberg

7 years

Absolutely floored to see @RichardDawkins 's tweet about the book I wrote with Jennifer. The Selfish Gene truly inspired me as an undergrad

@RichardDawkins

Richard Dawkins

@RichardDawkins

7 years

Flight from London to NY pleasantly filled by Jennifer Doudna’s book, “A Crack in Creation”, cover to cover. What a superb scientist she is.

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@shsternberg

Sam Sternberg

3 years

Excited to see this paper published! Beautiful work from Mike, Popo, and @JoePetersLab on target site selection by CRISPR-transposons using cryptic, privatized guide RNAs. Congrats!

@CellCellPress

Cell

3 years

New Article now online: Guide RNA Categorization Enables Target Site Choice in Tn7-CRISPR-Cas Transposons

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Sam Sternberg

4 years

The pace of scientific progress with SARS-CoV-2 / COVID-19 is incredible

@EricTopol

Eric Topol

4 years

There's been recent reports of the crystal structure of #COVID19 . But now the crystal structure of the antibody, derived from a patient who recovered, w/ implications for vaccine devp't So proud of my colleagues @scrippsresearch h/t @hholdenthorp

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Sam Sternberg

5 years

Been checking out Jiankui He's website. His academic training is in single-cell sequencing (no publications related to CRISPR & assisted reproduction), and his embryologist collaborator, Jinzhou Qin, received his PhD in 2016 --> just 2 years ago! Insane

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@shsternberg

Sam Sternberg

2 years

What an amazing visit! I am honored that you and your students welcomed me and my lab into your classroom this year, and I had a blast learning about your CRISPR experiments today. Let’s do it again next year!!

@WenWanso

Wendy Wansor

2 years

🧬 🔵➡️⚪️✅ Sharing our CRISPR experiment results with @shsternberg @WTClarkeMS @davecasamento @dharls1 @TerryRenna7 @jameshmccabe @jmiraglia31 #reallifescientist #autographs #proud ❣️

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Sam Sternberg

6 years

Can't wait to read this paper. Was wondering when we'd see a paper from David Liu's lab applying PACE to Cas9 directed evolution. Interested to see what we can learn about how PAM recognition is tied to DNA interrogation & specificity. Many times was asked about a "PAM-less Cas9"

@Nature

nature

6 years

The modifications could make the enzyme, called xCas9, a lab staple.

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Sam Sternberg

3 years

Watching Nobel Prize Award Ceremony live... so cool.

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Sam Sternberg

5 years

Stunning. The scientist purportedly responsible for the birth of world's first gene-edited babies describes the work. "I understand my work will be controversial, but I believe families need this technology and I'm willing to take the criticism for them."

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@shsternberg

Sam Sternberg

6 years

Super cool! Somewhere between 260 million to 7 billion viruses per square meter are deposited *every day*, after being aerosolized and traveling through the free atmosphere. Wowza.

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Deposition rates of viruses and bacteria above the atmospheric boundary layer

The ISME Journal - Deposition rates of viruses and bacteria above the atmospheric boundary layer

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Sam Sternberg

3 years

Huh? The dCas9 mutation in Qi et al. (2013) was actually H841A, not H840A? But S. pyogenes Cas9 has isoleucine at position 841. I'm confused... @stanleyqilab h/t @joeBondyDenomy

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@shsternberg

Sam Sternberg

5 months

Excited to see this out -- congrats! (Now I have a link to share, as I keep telling colleagues to think (more) carefully about reaction buffer conditions! :)

@dsashital

Dipa Sashital

5 months

I'm excited to share our findings on a metal-dependent specificity switch for #CRISPR -Cas12a! We profiled the specificity of Cas12a at four Mg2+ concentrations and observed striking differences in mismatch tolerance at lower, physiological conditions 1/n

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@shsternberg

Sam Sternberg

8 years

In case you've been living under a rock and don't know what #CRISPR is, check out my #TEDMED talk - now online!

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@shsternberg

Sam Sternberg

7 years

Whoa, this is exciting (and humbling)

@BNBuzz

Barnes & Noble

7 years

Rising fast up our bestseller ranks - trending biology book "A Crack in Creation", a history of "gene editing" tech:

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@shsternberg

Sam Sternberg

2 years

Exciting stuff - congrats everyone! It was one of my favorite talks at the conference (and reminded me of Martin and Krzysztof’s tag team talk in 2012). Bonus was hearing @irma_querques present it again to our lab on Friday in person.

@MartinJinek

Martin Jinek

2 years

As presented @crispr2022 , we are excited to share our new data on CRISPR-transposon systems. Huge thanks to the fantabulous team @Michael__Sch @irma_querques @OberliSeraina and @ChrisChanez89 for the amazing work!

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Sam Sternberg

7 years

Can't believe it's already my last day at @CaribouBio ! Had a blast here, learned a lot. On to getting the Sternberg Lab off the ground...

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@shsternberg

Sam Sternberg

5 years

What I don't understand: message of article is again to prevent genetic disease, and core principle #2 is "Only for serious disease." But there are other ways to prevent vertical transmission of HIV, so their clinical use doesn't even seem to follow their own guidelines!

@CRISPRjournal

The CRISPR Journal

5 years

New in @CRISPRjournal : He Jiankui’s group proposes a set of 5 core values to frame, guide, and restrict clinical applications of gene editing technologies. #GeneEditSummit

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@shsternberg

Sam Sternberg

4 years

After a fantastic symposium this morning on coronavirus/COVID-19 research happening at @ColumbiaMed , and a chat just now with some students leading a huge effort to review new literature coming out, I am feeling inspired and honored to be part of this community. @Columbia_CRAC

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Sam Sternberg

4 years

Beautiful work, Joe et al., wow! We had a great lab journal club digging deep into this work yesterday and really enjoyed the manuscript - congrats! CRISPR-transposons continue to amaze...

@JoePetersLab

Joseph E. Peters

4 years

Guide RNA categorization enables target site choice in Tn7- #CRISPR -Cas transposons Allows access to new plasmids and phage for horizontal transfer while keeping long-term memory of safe sites in bacterial chromosomes

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Sam Sternberg

4 years

@MartinJinek @doudna_lab @manue_lab Hmm... if it took a Nobel Prize for tweet #1 , what will it take for #2 ? ;) Good to see you on here Martin!!

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Sam Sternberg

4 years

@IsraelF96135088 @biorxivpreprint On the other hand, I'm questioning the value and benefit of making our work available as a preprint in advance of publication, when multiple groups essentially copied our study and tried to beat us to press. Thoughts? What lesson do we take away from this, for next time? 3/3

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Sam Sternberg

6 years

New book - pretty stoked :)

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Sam Sternberg

5 years

A new article about CRISPR and gene editing from @WalterIsaacson gives an exciting preview of what I'm sure will be a fantastic (future) book on the topic. Exciting to see our recent work mentioned on CRISPR-containing transposons, or 'jumping genes.'

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Should the Rich Be Allowed to Buy the Best Genes?

Walter Isaacson on CRISPR.

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Sam Sternberg

6 years

Sternberg Lab ( @SternbergLab ) trip to see Rampage!! CRISPR makes it to Hollywood.... we can only dream...

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Sam Sternberg

4 years

Been feeling a bit down/anxious with all the depressing news lately... this is helping. (Thanks to NPR for the album review earlier) Mixing Colours by the Eno brothers

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Sam Sternberg

8 years

Another day, another #CRISPR breakthrough. Cpf1 + DNA, novel 2nd nuclease domain. Awesome!

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Sam Sternberg

3 months

Check out Diego's 🧵and excellent protocol on how to best harness CRISPR-associated transposases (CASTs) for bacterial genome engineering. We hope it will be a great resource for the community!

@tehhoyen

Diego Rivera Gelsinger

3 months

Never too late to share @SternbergLab 's first Nature Protocols! Interested in editing your favorite bug? 🦠 Check out our protocol describing a novel and versatile CRISPR-associated transposase (CAST) system for targeted insertion of large DNA payloads into bacterial genomes.

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Sam Sternberg

5 years

Amidst this crazy CRISPR news cycle, what a welcome break to participate in #SkypeAScientist and video-conference with a high school classroom from Burns Flat, OK. Great questions, enthusiasm, interest in the research, the technology, the ethics, and how to become a scientist!

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Sam Sternberg

4 years

Help: Is there any way in the new Pubmed to have the results automatically sorted by "Most recent," instead of (annoyingly) having to select this from the Display Options dropdown every time??

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Sam Sternberg

9 months

And check out some the amazing BioArt the students created! Thanks to @ATinyGreenCell for sharing his samples with us!

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@WenWanso

Wendy Wansor

9 months

@shsternberg @Columbia @SawickiScience @jameshmccabe @dharls1 🧬 Extracting DNA from 🍓 & 🍌 at Cutting Edge Science Camp 🧬 #welovescience #funwithfriends #youngscientists 🧑🏽‍🔬👩🏻‍🔬

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Sam Sternberg

4 years

Some exciting synergy with another preprint just out from @doudna_lab and @BanfieldLab ... congrats to all the authors involved in that story! It's clear that RNA-guided integrases are going to be a powerful new addition to the genome engineering toolkit

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Sam Sternberg

2 years

An elegant and creative study to convert Type V-K CAST systems from copy-and-paste into cut-and-paste transposases - congrats! (I'm still very curious what biological factors drove different transposition behaviors... why replicative sometimes, non-replicative others?)

@connorjtou

Connor Tou

2 years

Today in @biorxivpreprint , we describe the development of HELIX, an advance that markedly improves properties of type V-K CRISPR-associated transposases (CASTs) for programmable, cut-and-paste, multi-kilobase DNA integration. 1/14 🧵👇

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Sam Sternberg

4 years

Congrats on this excellent story, Josh et al.! Who would've thought that Type II CRISPR-Cas systems encoded a natural single-guide RNA all along! Another cool example of Cas effectors being repurposed for non-canonical (non-nuclease) fxn, in this case transcriptional repression

@jwmodell

Joshua Modell

4 years

Excited to announce the maiden voyage of the Modell lab, led by the outstanding @reworkman0 and @tejapammi . We found S. pyogenes Cas9 uses a natural single guide RNA, a conformation of the 171nt tracrRNA, to transcriptionally repress its own promoter.

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Sam Sternberg

7 years

This might be one of the coolest tattoos I've ever seen! (h/t to @mitchellrayoco1 and the guy who let him take the picture)

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Sam Sternberg

7 years

Hype and stocks aside, I am skeptical (at best) about @naturemethods article on CRISPR off-target mutations, and suspicious (at worst) 1/n

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Sam Sternberg

5 years

Prediction: 2018 will be remembered as the landmark year in which humans first began rationally modifying the genetic material of their offspring, and in doing so, taking direct control over evolution

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Sam Sternberg

6 years

Holy crap. Had no idea a Hollywood movie was coming out featuring CRISPR. And starring The Rock no less. His tweet might top Ashton Kutcher's for funniest/coolest CRISPR celebrity shout-out

@TheRock

Dwayne Johnson

6 years

Great RAMPAGE review from my fellow Science majors. We wanted to utilize real CRISPR technology as our scientific backdrop. Of course I still screw up what this acronym means. 🤷🏾‍♂️

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Sam Sternberg

6 years

Excited to be co-hosting an RNA Salon at Columbia University, together with colleague Laura Landweber. Monthly seminars featuring research across Columbia's campuses focused on RNA! @RNASociety @Columbia

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Sam Sternberg

5 years

Thanks for the wonderful News and Views, Fyodor! I had no idea you were writing this, and I couldn't be happier. I may just cite this more often than our own paper... ;)

@UrnovFyodor

Fyodor Urnov

5 years

Our future of "Editing as medicine" has a new actor (only an understudy for now): a CRISPR "borrowed" from bacteria by its parasite. Mother Nature truly rocks in how creative she is - as shown in beautiful, and real-world-resonant, work by @shsternberg .

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Sam Sternberg

5 years

Interesting that most of this video focuses on using gene surgery to avoid children suffering from genetic disease. But the edit made to Lulu and Nana's genome was (apparently) an elective mutation of CCR5 to achieve HIV resistance, not a change to prevent an unavoidable disease

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Sam Sternberg

6 years

Check out these sweet stamps I bought from @USPS ! Digging the theme

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Sam Sternberg

6 years

"If the Model T was prone to overheating, Crispr Classic is prone to overeating." 🙄 Is anyone else slightly irked that journalists are now often writing "Crispr" instead of "CRISPR"? I know NYT has a silly policy on acronyms, but Wired too? And WSJ?

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Sam Sternberg

1 year

I had a blast Chris! East Meadow has some really bright 7th graders and incredible teachers. I’m excited to keep our partnership going and hear about their CRISPR experimental results in June!

@SawickiScience

Chris Sawicki

@SawickiScience

1 year

7th Graders at Woodland and Clarke met with Dr. Sam Sternberg to make connections from the classroom to the research lab! #CRISPR #nextgenscience @ms_woodland @WTClarkeMS @TerryRenna7 @WenWanso @dharls1 @emeadowschools @shsternberg

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Sam Sternberg

6 years

Speaking of RNA, @hauke_hillen gave a fantastic seminar here yesterday on his studies of mitochondrial transcription. What a pleasure to host him and spend a few days catching up and talking science. We were @doudna_lab buddies back in 2012!

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Sam Sternberg

7 years

Excellent video animation from @naturemethods about various CRISPR-Cas9 tools and how they work. h/t @stephenfloor

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Sam Sternberg

1 year

Thrilled to have you join the lab Egill! I’m excited for the science we’ll do together 👍

@EgillRichard

Egill Richard

1 year

Officially starting my Post-Doc @Columbia in @shsternberg 's lab. Exciting science on the way, with a terrific team, in an amazing city!

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Sam Sternberg

5 years

Beautiful sunrise over New Jersey this morning, viewed from Manhattan. Oh wait, that’s the moon! 🌞🌝🤷🏻‍♂️

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Sam Sternberg

7 years

Just had a new fridge delivered to my house. Only one way to properly break it in... #crispr #notcrisper

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Sam Sternberg

7 years

So excited to read this! Fantastic poster at conference, probably even better paper Kinetics of dCas9 target search

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Sam Sternberg

8 years

How big is CRISPR? Craigslist Missed Connection mentioning it just popped up in my Google Alert! Hope she finds him

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Sam Sternberg

1 year

Congrats Prashant et al.!! There’s a lot of interest here at Columbia about this work — I’ll be in touch about getting you over here for a seminar!

@_prashantbhat

Prashant Bhat

1 year

In our new pre-print, we demonstrate that 3D genome organization around nuclear speckles controls the efficiency of mRNA splicing. Check out my thesis work @Caltech in this thread by @mitchguttman ! @4DNucleome #Genomics

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Sam Sternberg

5 years

Just now remembering that the book I wrote with @jadoudna on CRISPR gene editing is presently being translated into Chinese for 2019 publication, and the publisher is expecting us to author a new forward. Today's development adds an interesting new element to that....

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Sam Sternberg

2 years

Yes, I (and the whole lab) have been excitedly anticipating this article being published - a big congrats to the authors!!

@IsraelF96135088

Israel Fernandez

@IsraelF96135088

2 years

👇🏽👇🏽👍🏽 Ecoli TnsC!!! A classic protein finally characterized structurally. Bravo @alba_guarne et al!!!

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