Sebastian S. Cocioba🪄🌷 @ATinyGreenCell Twitter profile

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Sebastian S. Cocioba🪄🌷

4 months

Okie dokes, I set up my YouTube channel. Currently testing upload resolutions. Please subcribe and click da bell since this is where my videos will live. 💚

Sebastian Cocioba

Amateur Plant Biologist Aspiring Flower Designer Degreeless Heathen operating out of a home lab

www.youtube.com

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

2 months

Went with my partner upstate and the AirBnB host's cat took us for a guided hike along the Appalachian trail. Apparently this is what she does with every guest. She would complain when we took a wrong turn off the trail and knew the way back. Amazing cat. Would apocalypse with.

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@ATinyGreenCell

9 months

It's so wild that we can "see" molecules now. Humans can do so many amazing things when we're not being miserable monsters to each other. I have hope tho.

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@ATinyGreenCell

1 year

Salad spinners are the best plate centrifuges.

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@ATinyGreenCell

3 years

I love my lil hand made agarose gel transilluminator. Really proud that I circumvented a really shitty patent essentially owning the idea of "perpendicular illumination". Well, it's all freehand and no PCBs or glass lenses so take a hike. Will make for anyone. $64 + shipping!

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@ATinyGreenCell

2 years

I wish biology undergrad curricula had more statistics. Every bio major should have 2 semesters of bioinformatics with applied statistics. Learning basic R before graduating would be stupidly powerful.

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@ATinyGreenCell

10 months

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

2 years

Here is an alternative to BioRender that is TRULY FREE and actively maintained. Over 1500 vectorized images sorted by license type (all creative commons or MIT). Fully editable icons and soon they will launch an Inkscape library. Please RT.

GitHub - duerrsimon/bioicons: A library of free open source icons for science illustrations in...

A library of free open source icons for science illustrations in biology and chemistry - duerrsimon/bioicons

github.com

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@ATinyGreenCell

2 years

ggplot2 has gone too far.

Gene McAvoy

@SWFLVegMan

2 years

Natural variation in chicken egg colors

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@ATinyGreenCell

1 year

My cozy lil research nook. It's not much but it's got everything I need to explore the living world.

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@ATinyGreenCell

2 months

My partner takes some lovely cat pics. She makes them pose so regal.

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@ATinyGreenCell

4 months

CRISPR has gone too far

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@ATinyGreenCell

3 years

To review such a magnificent paper...

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@ATinyGreenCell

2 years

Why are so few rich people participating in science as a hobby? What changed from "bored victorian rich ppl musing about nature" to "science as a profession" that made the idea of having a baller home lab not in vogue? Asking for a broke amateur biologist looking for funding, lol

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@ATinyGreenCell

1 year

Teachin my mom how to nannodrop. She'll make a fine research assistant soon.

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@ATinyGreenCell

2 years

The fact that there is a step in traditional molecular cloning where you physically cut DNA out of a gel will forever remain as one of the most tangible examples of sci-fi ever. This literally never gets old. So cool!

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@ATinyGreenCell

2 years

Cells are ready and our Etsy store is now stocked with these for purchase!!! No MTA, no red tape, and all just $20/plasmid or less. Shipping as agar stabs in E. coli BL21(DE3) cells. All proceeds go directly into making more tools for the community. Enjoy!

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@ATinyGreenCell

2 years

Still very much in disbelief that two degreeless heathens in a home lab with no formal training managed to produce a reference quality bacterial genome of a really hard-to-crack microbe and that our assembly is now THE representative genome of that organism. Amateur biology FTW!

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@ATinyGreenCell

4 months

This is honestly the most intimidating thing in biology for me. What even is this thing? Is this where the fleeb juice is stored? It takes in blublubs and processes them to export as glublububs. Such elegance. Much ordered structure. Very why god why.

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@ATinyGreenCell

2 years

Who needs Infinity Stones anyway...

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@ATinyGreenCell

1 year

If this is real I am going to cry 😭😭😭😭😭😭😭😭

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@ATinyGreenCell

3 months

Mmmm, future tech. I got these cool sodium ion batteries in 18650 size for about $1.50/piece. Rated at 1300mAh and 3.90Wh. Nominal voltage of 3.0v. Extremely cold resistant. No thermal runaway. Far more sustainable. Excited to design using salt-tek, lol.

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@ATinyGreenCell

10 months

Wanna generate metabolic maps of sequenced microbes? Ever heard of the Japanese KEGG Pathways database? Here's how I use this free tool to explore the clockwork of my bug of interest. 🦠🛠🧵 1/6

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@ATinyGreenCell

2 months

@jaimecor_94 Tbh I did not see or meet the host and emails were delayed to after the cat hung out with us, so...

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@ATinyGreenCell

1 year

I'm making fresh agar stabs of these if anyone wants them for their lab or class. $20/plasmid. Open source. DM me postal and email address and I'll ship em by next friday :) EforCP red YukonOFP orange DasherGFP yellow FuGFP fluoro green AeBlue blue TsPurple purple All kanR

Mark O. Martin

@markowenmartin

1 year

More closeups of the weird multicolored bacteria situation. I used plasmids by @ATinyGreenCell , in E. coli. It's an exercise for students to learn how to streak one thing from a mixture. I mixed cultures with the different colors---so not cotransformation? Fascinating! @profvrr

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@ATinyGreenCell

2 months

This pathway leads to a bad trip, it seems...

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@ATinyGreenCell

2 months

Here's the listing if anyone wants to escape to the mountains and hike with a cat. Peek the reviews for more cat pics.

Rental unit in Philipstown · ★4.88 · 1 bedroom · 1 bed · 1 bath

Foxglove Farm

www.airbnb.com

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@ATinyGreenCell

9 months

Gelposting.

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@ATinyGreenCell

2 years

My dad died today and my world just imploded. Might go dark for a bit to sort the everything. Apologies to any I promised meetings with this week; will resume later. Time to dig up some strength from ye olde reserves. Love y'all. 💚

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@ATinyGreenCell

1 month

Just. Share. Your. Plasmids. Enough with the MTAs. It's the age of synthesis. The material doesn't matter and all you are really doing is saving your peers time and money. The thing, once constructed, is an infinitely renewable resource. Just share it. Don't be weird.

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@ATinyGreenCell

2 months

Since my big autoclave died, I've been using this InstantPot pressure cooker exclusively for all my sterile work. Has not given me contam once and I often run various antibiotic-less media. I set it to max temp n pressure. Set to Pressure Cook mode 30mins.

Instant Pot Duo Plus 9-in-1 Electric Pressure Cooker, Slow Cooker, Rice Cooker, Steamer, Sauté,...

Easy to use, easy to clean, fast, versatile, and convenient, the Instant Pot Duo™ Plus replaces 9 kitchen appliances: pressure cooker, slow cooker, rice cooker, steamer, sauté pan, yogurt maker, rice...

www.amazon.com

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@ATinyGreenCell

6 months

Trying a quick gel purification trick I saw in a paper recently (reference needed). Poke 0.5mL tube with 22 guage needle. Stuff with ~50uL of cotton. Tamp it down. Wash with 100uL dh2o. Keep collection tubes for reuse. Autoclave washed tubes. Load gel slab. Spin 14k 10mins. Done.

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@ATinyGreenCell

1 year

In case anyone missed the original post a while back, here is a *FREE* BioRender equivalent that has image assets compatible with InkScape and other illustrator platforms.

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@ATinyGreenCell

2 months

What an excellent figure to demonstrate hybrid vigor and the segregation of this in subsequent progeny.

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@ATinyGreenCell

9 months

Nine PCR optimization runs later and I got my bands. DMSO + very specific annealing temp + Taq. Q5 and PrimeStar failed miserably. Some slight secondary amps, but whatever. Biology is hard.

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@ATinyGreenCell

5 months

I do it all for the meta.

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@ATinyGreenCell

1 year

Excellent glycolysis graphic. The use of colored circles really helps track carbon and phosphate fate. Found this a while back on rando google image search so unknown source. Please rev image search for citation. Enjoy!

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@ATinyGreenCell

2 years

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@ATinyGreenCell

1 year

I love these autoclavable "petri dishes". They are 50mm bead boxes used for storing fly fishing tackle, but are made of polypropylene and have snap fit lids. Been using these for 3 years now and never turning back. Yay, minimizing disposable plastic waste!

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@ATinyGreenCell

2 years

My only read claim to fame is that I own one of these...

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@ATinyGreenCell

11 months

I'm out of town and needed to start an overnight culture to miniprep tomorrow when I get back, so I'm walking my mom through the steps. So proud of her 💚

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@ATinyGreenCell

2 months

At times she judged the crap out of us for being too slow. I got a camera roll full of judgement, lololol

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@ATinyGreenCell

1 year

I made a handy tube tumbler that will be part of an upcomming publication with @SarraceniaMason . If you want a headstart on printing this ultra quiet device, see the link below. Sub-$50 in parts and modular. Use TinkerCAD link to modify. Sound on for slosh

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@ATinyGreenCell

1 year

We were so close! Must be a reason this mutation wasn't selected out as a fitness drop. No scurvy...but at what cost?

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@ATinyGreenCell

6 months

Finally. Toy-grade PCR machines that actually work. So tired of the pretentiousness surrounding thermocyclers. It's a hot-cold-hot machine. Trivial with modern materials. No reason it should ever be so expensive. Ever. Thus one retails for $245.

WATSON 4-Well Portable Thermocycler

www.daigger.com

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@ATinyGreenCell

2 years

Looking forward to this! Maker of the PocketPCR that fits in the palm of your hand.

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@ATinyGreenCell

4 months

My happy place.

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@ATinyGreenCell

1 year

If anyone has a Qubit fluorometer and does not want to pay Thermo twelve arms and thirty legs for the broad range kit, here is an alternative.

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@ATinyGreenCell

2 years

Ah, yes. The secret book we've all read...

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@ATinyGreenCell

24 days

I love this stuff! Becomes like putty in boiled water and then stiffens rock hard as it cools. Excellent manual sculpting material and infinitely reusable. Good machinability too. Scraps can be melted together. Takes dyes well too. Cheap.

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@ATinyGreenCell

1 year

Prof at uni in "global south": yo can I get a stab of these plasmids? I wanna use it for an outreach event... Me: sure thing. *mails stabs* Prof, months later: you single-handedly increased outreach to hundreds of students while our uni refused to help ...😒

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@ATinyGreenCell

8 months

Fluorescent proteins are so pretty and so very useful. Testing out some new expression elements for my client. Fun times!

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@ATinyGreenCell

1 year

One of my most prized possessions. This is the pencil I wrote with in kindergarten, where my teacher yelled at me for wanting to use every last bit of it and not get a new one. It means a lot to me for many reasons, but also because it fits perfectly in a 1.5mL tube. Fate? Fate.

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@ATinyGreenCell

1 year

THE INITIAL #BlueSoup STREAK PLATES TURNED BLUE IN THE FRIDGE!!!!

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@ATinyGreenCell

1 year

#BLUESOUP LETS GOOOOOOOOO Plated on King's Agar A and B (pyocyanin and fluorescein ind respectively) and will incubate at 30°C for 24 to 48hrs. Resuspended in LB and then serially diluted in 10x steps to 10,000x. Plated 10uL per dish. Rest stored in back of fridge at 4°C. 1/2

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@ATinyGreenCell

11 months

My proudest moment in plant protoplast isolation. Textbook results. I could never get it this perfect again, but it remains an great feeling. One day...

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@ATinyGreenCell

1 year

If anyone that either works with fuGFP or bought one of my plasmids wants to visualize the green glow, just use any 395nm flashlight off amazon or ali express. No filter. Looong stokes shift. Here is the flashlight I use:

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@ATinyGreenCell

3 years

@ellykaybee Nothing more empowering than taking control of your life path and doing what you feel keeps you sane, happy, and brings a sense of purpose to your work. Exciting times ahead!

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@ATinyGreenCell

7 months

🎶these are a few of my favorite thingggs🎶

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@ATinyGreenCell

2 years

OMGOMGOMG our genome assembly for Deinococcus radiophilus is now the representative genome of that organism on genbank!!! Our little amateur biology lab did a thiiiiiing 🥰

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@ATinyGreenCell

1 year

Alright, we got another pilot #BlueSoup experiment underway to test if the glowy bugs make blue on soft cheese! A few articles suggest that Pseudomonas fluorescens can make cheese blue so I put some mozz in 12 well plates + 1mL of mozz broth + 30uL of BS1-A cells. 🧵1/4

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@ATinyGreenCell

2 months

Just a quick thank you to everyone that has looked past my degreelessness and respected me as a member of this research community. You have no idea how much this means to me. I hope to pay it forward and be helpful in whatever way I can. Your kindness is a blessing. 💚

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@ATinyGreenCell

3 years

Finally, I finished drafting my take on a digital firefly. About the size of a large adult, this small circuit is solar powered (charges a tiny supercapacitor) and flashes at dusk for a minute or two...forever. Tricky to route but programmable via ATTiny10 and no lithium batts!

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@ATinyGreenCell

2 years

Behold my child, Scarlett. 🧵 It all started with a 6mm leaf disk from seedlings sown in-vitro. 1/15

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@ATinyGreenCell

6 months

I have failed...but I couldn't be happier. A small thread on recent experiments 🧬🔀🧵 1/n

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@ATinyGreenCell

1 year

How many kilograms of taq polymerase do you think has been used since the invention of PCR?

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@ATinyGreenCell

5 months

@AlHendiify Not sure how, but Vinny from the NYC water authority managed to get the East and Hudson the cleanest its been in decades. We got cold coral growing again!!! Sponges returning too. Big fat red ones. Nature is healing via la cosa nostra????

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@ATinyGreenCell

6 months

I finally found the reference paper for this technique!

A Quick, Cost-Free Method of Purification of DNA Fragments from Agarose Gel

In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or...

www.ncbi.nlm.nih.gov

Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

6 months

Trying a quick gel purification trick I saw in a paper recently (reference needed). Poke 0.5mL tube with 22 guage needle. Stuff with ~50uL of cotton. Tamp it down. Wash with 100uL dh2o. Keep collection tubes for reuse. Autoclave washed tubes. Load gel slab. Spin 14k 10mins. Done.

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@ATinyGreenCell

4 months

Alright. My job is done. Closed up the lab, my research contract, and my time here at neoplants like a professional. Met some amazing people, grew as a scientist, and made some friendships that will surely last a lifetime. I'll miss this place. On to the next adventure!

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@ATinyGreenCell

6 months

Man...I have no words.

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@ATinyGreenCell

4 months

Our paper is now online!!! So happy to see it finally in print. Another lil contribution toward open science and hardware accessibility. It takes a village, and a few 3D printers too. A very fun project led by @SarraceniaMason . Enjoyyy 💚

Toward an open‐source 3D‐printable laboratory

Premise Low-cost, repairable lab equipment is rare within the biological sciences. By lowering the costs of entry using 3D printing and open-source hardware, our goal is to empower both amateur and...

bsapubs.onlinelibrary.wiley.com

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@ATinyGreenCell

3 years

Amateur biological research as escapism with societal benefit as a byproduct. Learn about the natural world, generate new knowledge, explore your personal curiosities, and publish your findings for the benefit of us all. Not a terrible way to spend one's free time...

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@ATinyGreenCell

1 year

Found a FREE and open source browser-based tool for making sequence logos to show base frequency with respect to position in multi-seq alignments as well as standard bioinfo file types. You can also download code 2 run larger jobs locally. Bookmark this!

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@ATinyGreenCell

11 months

ACCEPTED PENDING REVISIONS!!! 🥳📝🥳📝🥳📝🥳

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@ATinyGreenCell

11 months

MY EXPERIMENT IS FULLY FUNDED!!! Thank you all SO MUCH. This is such a dream come true and I look forward to the day robots like this one show up in many classrooms around the world. Experimental evolution is one of the coolest things we can engage. SO!!! EXCITED!!!

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@ATinyGreenCell

3 years

Nature's design pallette is sublime.

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@ATinyGreenCell

2 years

I got an interview on Saturday with designer plant synbio company! If this pans out, I'll have a job later this year where I can help make functional and aesthetically pleasing houseplants from my home lab...with an actual real path to deregulation and market. Flower design! 🥰💚

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@ATinyGreenCell

3 months

@8teAPi Extreme UV photolithography is the most advanced industrial process humanity has ever devised. Ever. Even the wizards in Taiwan struggle at that bleeding edge. Making chips has very little room for tolerances given the whole mass production + nanofab thing.

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@ATinyGreenCell

7 months

I love these things. Always tempted to streak and homebrew, or at least poke around in its genome. It's a GRAS species and strain so...

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@ATinyGreenCell

3 months

Anyone who insults a person for wanting to learn more about our world, regardless of age, does not deserve cognition. Full fucking stop.

Dan Nouement

@nomanautomata

3 months

You're learning the math I learned at 14 years old, I'm learning the social skills you learned at 14 years old, we are NOT the same

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@ATinyGreenCell

1 year

TIL that modern celery was made through selection for petiole elongation and thickening. I never looked close enough to realize that the stalks we eat are celery leaf petioles!

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@ATinyGreenCell

5 months

Explain yourselves.

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@ATinyGreenCell

3 years

If we start a video series called The Joy of Cloning where we bob ross our protocols in a relaxing manner, would you watch? Flowers and Phages and Microbes

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@ATinyGreenCell

2 years

Manuscript accepted!!! Megathanks for @bielleogy for letting me contribute to their story. My first peer reviewed paper, which hits *very* different as an amateur biologist. Looking forward to many many more. 💚

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@ATinyGreenCell

1 year

My inbox exploded with chromoprotein orders! Will ship out as many as I can this week and then order more gasketed tubes to make more agar stabs next week. Y'all just funded a full year of research! 🤯💚 Thank you so much!

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@ATinyGreenCell

1 year

Gel looks great! Ladder, 1 before, 1 after, 2 before, 2 after, ladder. Good enough for me. Adopting this protocol from now on!

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@ATinyGreenCell

1 year

@timrpeterson Biology lags behind software because biology is not dead silicon that remains unchanged, where the parts are discrete and fully defined, where whole systems are simulatable and the iteration cycle is seconds not years. Software ≠ hardware ≠ wetware. Myopic is an understatement.

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@ATinyGreenCell

2 years

Another fresh batch of chromoprotein starters for today's agar stab session. Managed to finish cloning a yellow too! Looking forward to forest green soon. Microbiology can be so satisfying :)

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@ATinyGreenCell

2 years

Someone just told me "your enthusiasm is a good career move but don't overdo it" so I am now forced to double down on said enthusiasm for science and will continue to enjoy my biological practice in the manner that represents my style best. This is the second time I got such a DM

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@ATinyGreenCell

1 year

Whatever grew on the #BlueSoup colony plates overnight glows under UV, but only on King's Agar B! That particular media is used to tease out fluorescein expression in pseudomonads. What are the chances that the same cell line expresses fluorescent AND blue pigments? 🧵1/3

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@ATinyGreenCell

1 year

I can't believe this silly #BlueSoup adventure made it to IFLS, lol!

Microbiologist Investigates After Her Beef Soup Turned Blue In The Fridge

Here's what's going down in the blue beef soup saga.

www.iflscience.com

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

3 months

So excited to build this! NO ANTIBIOTICS!!!

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

1 year

Me, preparing agar stabs for everyone while thinking about how Etsy permanently shut down my storefront for distributing aseptic deregulated living cells while allowing raw pond scum and random dirt sellers to thrive:

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

1 year

One thing I ask if you use my chromoprotein plasmids in educational settings is to remind your students that these genes came from our oceans, gifts from the great reefs, and it's up to us to protect these envrionments. Keeping our oceans healthy is of the utmost importance. 🌊🙏

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

1 year

Old but gold

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

4 months

This makes me so happy. A falcoponic berry 🥰

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

1 year

If you are an amateur scientist, please do yourself the biggest favor ever and PUBLISH YOUR WORK. Have a blog, upload to genbank or other repos, document your efforts in an archival manner. Zenodo, figshare, etc. Don't let your work die with you or a change in your life path. 🙏

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

1 year

Lavoisier's laboratory. The world's first scientific bioreactor.

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

3 months

🤌 corone 🤌

James Schnable

@szintri

3 months

"The diagram visualizing the WUSCHEL/CLAVATA signaling pathway has been generated. This figure aims to convey the complex interactions within this crucial regulatory network for stem cell maintenance in the shoot apical meristem of plants." 🤣

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

10 months

How is this man getting funding?! We don't even know the causal mechanism of the god damn disease and this guy wants to GENE EDIT it away? I...I can't.

Jiankui He

@Jiankui_He

10 months

I am proposing a research project "Human embryo gene editing to protect against Alzheimer’s disease"

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Sebastian S. Cocioba🪄🌷

@ATinyGreenCell

1 year

Happy to announce I got a remote gig with !!!! Off to Paris for a 2 week meet and greet and proper onboarding. More details later, including career opportunities for academics/PhDs looking to shift to industry. So excited to work with Pothos!!!

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