Cryo-EM analysis of Cas7-11, the III-E CRISPR-Cas effector nuclease, provides mechanistic insights into the RNA-guided target RNA cleavage and enables rational designing of a compact variant capable...

Nature Communications - CRISPR RNA-guided endonuclease Cas9 recognizes and cleaves the double-stranded DNA complementary to the RNA guide. Here the authors use high-speed atomic force micropcopy...

The type III-E CRISPR-Cas7-11–Csx29 effector is an RNA-dependent nuclease protease that can be applied for RNA sensing.

Nature - Cryo-electron microscopy structures of Drosophila Dicer-2–R2D2 complexes with and without small interfering RNA reveal how the RNA is presented to Argonaute in the correct...

"I was sitting in the front, and I just heard this gasp from everyone behind me."

Takeda et al. report the cryo-EM structure of Cas12f in complex with a guide RNA and its target DNA. The structure reveals that two Cas12f molecules bind the guide RNA to form an effector complex and...

本記事は創薬 (wet) Advent Calendar 2019の14日目の記事です本記事は下記の環境に基づいて執筆されましたMacBook Pro (13-inch, 2016, Four …

Nakagawa et al. report the structures of the Cas13bt3-crRNA complex and the Cas13bt3-crRNA-target RNA complex. The structures revealed how Cas13bt3 recognizes the crRNA and its target RNA and...

The type III-E CRISPR-Cas7-11–Csx29 effector is an RNA-dependent nuclease protease that can be applied for RNA sensing.

Communications Biology - SpCas9 is a versatile genome-editing tool, but it is large and cannot be packaged efficiently in a AAV vector, limiting its application. The reported engineered...

Kurihara et al. report the cryo-EM structures of the Cas12c2–guide RNA complex and the Cas12c2–guide RNA–target DNA complex, providing mechanistic insights into the TN PAM recognition by Cas12c2....

東京大学 大学院理学系研究科・理学部の濡木理教授が第26回慶應医学賞を受賞情報です。

Communications Biology - The authors develop a platform (SATORI) that enables accurate and rapid detection of single-stranded RNA at a single-molecule level without a pre-amplification step. As a...

Communications Biology - An automated amplification-free digital RNA detection platform using CRISPR-Cas13a and magnetic bead technology implemented in a microchamber device demonstrates sensitive...

2020年ノーベル賞解説号

Takeda et al. report the cryo-EM structure of Cas12f in complex with a guide RNA and its target DNA. The structure reveals that two Cas12f molecules bind the guide RNA to form an effector complex and...

Nature Communications - CRISPR RNA-guided endonuclease Cas9 recognizes and cleaves the double-stranded DNA complementary to the RNA guide. Here the authors use high-speed atomic force micropcopy...

Hiroshi NishimasuProfessor, Research Center for Advanced Science and Technology, The University of Tokyo【Research Topics】 Exploration of new RNA-dependent en...

Nature Communications - The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights...

東京大学 大学院理学系研究科・理学部のプレスリリース情報です。

Nature - Accusations of impropriety feature in escalating dispute.

Nature Communications - Base editors can be limited by precision and the size of the target window. Here the authors test Cas9s that recognise alternative PAMs to obtain a series of high-precision...

Nature - The kit has been granted approval under ‘emergency use’ provisions, and should help to ease testing backlogs in the country.