Sorek Lab @SorekLab Twitter profile

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Sorek Lab

@SorekLab

2 years

SnapShot: Bacterial Immunity @Nitzan_T masterfully organized current knowledge on bacterial immune systems into a single page graphics Out today @CellCellPress :

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@SorekLab

2 years

Bacterial origins of human innate immune mechanisms Tanita Wein skillfully summarized current knowledge on how key components our own immune system evolved from bacterial anti-phage defenses Out today in Nature Reviews Immunology

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@SorekLab

2 years

Preprint: We report the discovery of >20 new bacterial defense systems! Some with homology to human immune genes A 4-year effort of the entire Sorek Lab, led by talented @AdiMillman , @Curiousphage , Azita Leavitt, @shany_doron and many more

An expanding arsenal of immune systems that protect bacteria from phages

Bacterial anti-phage defense systems are frequently clustered in microbial genomes, forming defense islands. This genomic property enabled the recent discovery of multiple defense systems based on...

www.biorxiv.org

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Sorek Lab

@SorekLab

4 years

After more than three decades of mystery, we are happy to report that we discovered the function of some of the coolest bacterial elements - retrons. Retrons function as anti-phage defense systems! See our new preprint @AdiMillman @AudeBer @AAvihail

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@SorekLab

5 years

On the origin of immunity Remarkable similarities between human and bacterial immune systems. Cyclic GMP–AMP signalling protects bacteria against viral infection Congrats CBASS team! Daniel Cohen @AdiMillman @GilAmitai @shany_doron #cGAS - #STING

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@SorekLab

2 years

Happy to see our paper out @nature : Phages confiscate signaling molecules produced by immune TIR proteins to cancel host immunity Structure of anti-defense "sponge" protein reveals a new class of antiviral signaling molecules: glycocyclic-ADPR (gcADPR)

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@SorekLab

2 years

Happy to share our paper out today @nature : TIR domains in bacteria protect from viruses by producing unique signaling molecules A surprising parallel between bacterial and plant immunity Congrats @gaofir , @GilAmitai and coauthors!

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@SorekLab

2 years

If Antoine de Saint-Exupéry was a microbiologist... Best farewell gift ever, from @Curiousphage

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Sorek Lab

@SorekLab

4 years

New in Nature: The evolutionary origin of the human STING protein is the bacterial CBASS anti-phage system. "STING cyclic dinucleotide sensing originated in bacteria"

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@SorekLab

1 year

Out @CellCellPress : Together with the Kranzusch lab, we characterized RADAR, a giant immune machine that blocks viruses via adenine deamination A complex of 96 protein subunits, 4 times heavier than the ribosome Congrats @B_DuncanLowey and @Nitzan_T !

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Sorek Lab

@SorekLab

4 years

A family of bacterial enzymes produce antiviral molecules to fight phage! These enzymes form the origin of Viperin, an important human antiviral gene. Check out our new paper:

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@SorekLab

2 years

Systematic discovery of phage determinants that trigger bacterial immunity Super talented @AAvihail 💫studied ~200 phage mutants that escape numerous defense systems Revealing key principles on how bacterial immune systems sense phage invasion🧫🧪

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Sorek Lab

@SorekLab

1 year

Out today @cellhostmicrobe : An expanded arsenal of bacterial immune systems Even if you previously read the preprint, don’t miss a major new result on the bacterial SEFIR domain, a homolog of human immune domains A thread 🧵 1/9

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@SorekLab

4 years

Abortive Infection: Bacterial Suicide as an Antiviral Immune Strategy Comprehensive review by @AnnaLopatina5 and @Nitzan_T

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@SorekLab

8 months

Proud to share our latest discovery: Bacteria conjugate ubiquitin-like proteins to interfere with phage assembly A new concept in antiphage immunity, and a mechanism for ISG15-like antiviral defnese in bacteria. Big congrats to talented @Jens_Hoer ! A 🧵

Bacteria conjugate ubiquitin-like proteins to interfere with phage assembly

Multiple immune pathways in humans conjugate ubiquitin-like proteins to virus and host molecules as a means of antiviral defense. Here we studied an anti-phage defense system in bacteria, comprising...

www.biorxiv.org

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Sorek Lab

@SorekLab

3 years

Now in Cell: Bacterial retrons function in anti-phage defense ! Excited to solve a decades-old mystery on the function of these RNA/DNA hybrid molecules Congrats @AdiMillman @AudeBer @AAvihail !

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Sorek Lab

@SorekLab

3 years

A link between bacterial and plant immune systems! TIR proteins protect from phage by generating signaling molecules that trigger cell death Similar molecule (cADPR variant) is produced by plant TIRs in response to pathogens Our paper: Congrats @gaofir !

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Sorek Lab

@SorekLab

1 year

Direct sequencing of proteins via nanopores? 🤯🤯🤯

Unidirectional single-file transport of full-length proteins through a nanopore

Nature Biotechnology - Full-length, unfolded proteins are slowly translocated through nanopores without enzymes and fingerprinted.

www.nature.com

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Sorek Lab

@SorekLab

7 months

Honored and humbled to receive the Max Planck Humboldt Research Award for 2023. Thank you to the Humboldt Foundation and the Max Planck Society for this honor! Excited towards collaborating with @JoergLab at @Helmholtz_HIRI and @v_hornung in LMU Munich

Max Planck-Humboldt Research Award 2023

Rotem Sorek receives the Max Planck-Humboldt Research Award 2023 and Amy Buck and Kandice Tanner are honoured with Max Planck-Humboldt Medals

www.mpg.de

Humboldt-Stiftung

@AvHStiftung

7 months

Glückwunsch an den Mikrobiologen Rotem Sorek zum Max-Planck-Humboldt-Forschungspreis 2023! 👏🎉 Die Max-Planck-Humboldt-Medaillen erhalten Amy Buck und Kandice Tanner. @maxplanckpress @SorekLab @WeizmannScience

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Sorek Lab

@SorekLab

2 years

The first symposium on the "beyond CRISPR" immune system of bacteria: Registration open! Feb 28th-Mar 2nd at the Weizmann Institute, Israel A fantastic line up of speakers + talks selected from abstracts. We have limited space, hurry up and register!

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Sorek Lab

@SorekLab

4 years

Our new paper is out in Nature Microbiology: Diversity and classification of cyclic-oligonucleotide-based anti-phage signalling systems

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Sorek Lab

@SorekLab

2 years

Pyroptosis is conserved between human and bacteria: caspase-activated gasdermins form pores and protect bacteria from phage Fantastic collaboration with the Kranzusch lab, now out in @ScienceMagazine Congrats @TanaWein & @algejohn !

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Sorek Lab

@SorekLab

6 years

Systematic discovery of antiphage defense systems in the microbial pangenome or 10 new ways a bacteria defends itself #newpaper

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@SorekLab

3 years

New defense system alert! Pycsar: Pyrimidine Cyclase System for Antiphage Resistance cCMP and cUMP are new immune signaling molecules! Congrats @Nitzan_T & @morehouse_ben ! A fantastic collaboration with the Kranzusch lab

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@SorekLab

8 months

I can't wait for the 2024 symposium on the "beyond CRISPR" immune system of bacteria, which will take place in Boston on April 16-18, 2024. Save the date!

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@SorekLab

1 year

Thank you to the HFSP Nakasone award, this is a great honor. Stoked and humbled to be included in the same list as the amazing scientists who received this award in the past

HFSP

@HFSP

1 year

And the winner of the 2023 HFSP Nakasone Award is... Rotem Sorek! We are honored to praise one of the top researchers in the Immunology field. Congratulations @SorekLab ! @WeizmannScience #HFSPNakasoneAward2023 #RotemSorek #HFSP

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@SorekLab

4 months

News on the Zorya defense system: anchors to the peptidoglycan and detects membrane disruption by phage. It has a flagella-motor-like device that rotates once activated. So cool! I've been waiting for insights on Zorya since we first described it 5 years ago. Congrats Tylor&co!

Phage Papers

@Phage_Papers

4 months

Structure and mechanism of Zorya anti-phage defense system

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Sorek Lab

@SorekLab

2 years

Immune NLR genes are conserved in bacteria and provide protection against phage. An incredible study. These bacterial NLRs recognize PAMPs that are conserved between phages and herpes viruses!

Prokaryotic innate immunity through pattern recognition of conserved viral proteins

Bacteria and archaea have innate immune receptors that recognize conserved viral proteins and prevent infection.

www.science.org

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Sorek Lab

@SorekLab

5 years

Forget Illustrator. This is the best rendition of the arbitrium communication system ever! Beautiful farewell gift made by @erez_zohar #bioart (we'll miss you!)

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@SorekLab

3 years

Antiviral defense via nucleotide depletion in bacteria New defense proteins, dCTP deaminase and dGTPase, degrade nucleotides during infection, starving the phage & halting replication Similar protein (SAMHD1) protects human from HIV Congrats @Nitzan_T !

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Sorek Lab

@SorekLab

5 months

Out now @Nature : our two joint papers with the amazing Kranzusch lab. We found how phages overcome the defense systems Gabija, Hachiman and Thoeris Congrats @ErezTzvi , @sadie_antine , Azita Leavitt, @amitai_gil and coauthors

Erez Tzvi Yirmiya

@ErezTzvi

5 months

By analyzing groups of genomically similar phages that showed differential sensitivity to bacterial defense systems, we discovered two anti-Gabija genes (gad1 and gad2), an anti-Thoeris gene (tad2) and an anti-Hachiman gene (had1).

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@SorekLab

11 months

CARD domains in bacteria! CARD domains are essential for recruitment of caspases during inflammasome activation and gasdermin-mediated pyroptosis in humans. We now found that CARD domains mediate anti-phage defense in bacterial gasdermin systems Congrats @TanaWein & @algejohn !

Tanita Wein

@TanaWein

11 months

I am beyond excited to share our new preprint!🤩 We find that CARD domains are an ancient component of innate immune systems conserved from bacteria to human, and that they are essential for gasdermin-mediated defense in bacteria! @SorekLab Kranzusch lab

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Sorek Lab

@SorekLab

1 year

We describe a new family of immune protein domains that break ATP to abort viral infection They protect bacteria against phages and also likely function in animal immunity Found in CBASS/NLR/pAgo, plus a new system: Detocs Congrats @FrancoisRousset !

François Rousset

@FrancoisRousset

1 year

I’m very excited to share the first results of my postdoc @SorekLab 🥳🤩🚨 We report a new family of immune effectors that is conserved across the tree of life.

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Sorek Lab

@SorekLab

5 years

Widespread Utilization of Peptide Communication in Phages Infecting Soil and Pathogenic Bacteria

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@SorekLab

5 months

Was honored and moved by the heartwarming ceremony in Berlin yesterday. Thank you to the Max Planck Society and the Humboldt Foundation! More than ever I am convinced that science is the strongest universal bridge to connect people and cultures

Patrick Cramer

@mpgpresident

5 months

Congratulations Amy Buck, Rotem Sorek, Kandice Tanner for receiving the Max Planck Humboldt Research Award 2023 yesterday in Berlin!

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Sorek Lab

@SorekLab

2 years

Our paper out today @NatureMicrobiol : Sirtuin (SIR2) domains in bacteria function in many anti-phage defense systems These domains degrade NAD+ once phage is sensed, depleting the cell of energy and halting phage replication

Nature Microbiology

@NatureMicrobiol

2 years

OUT NOW👉Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion A work by Sorek and colleagues @SorekLab @GarbJeremy

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Sorek Lab

@SorekLab

2 months

Mechanism of the Hachiman immune system finally deciphered A beautiful study from the Doudna lab shows that Hachiman is a genome integrity sensor Congrats @doudna_lab and colleagues!

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Sorek Lab

@SorekLab

5 years

Streamlining standard bacteriophage methods for higher throughput Tried and tested! Some great protocols by @k6logc from Polz Lab

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@SorekLab

3 years

Nice coverage of the "behind the scenes " - how we came to discover that Retrons are defense systems:

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Sorek Lab

@SorekLab

4 months

A new class of immune signaling molecules generated by TIR domains: histidine conjugated to ADP-ribose Check out our preprint: A fantastic collaboration with the Tamulaitiene and Kranzusch labs A 🧵 1/9

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@SorekLab

2 months

Preprint: Phages reconstitute NAD+ to counter bacterial immunity Many bacterial immune systems degrade NAD+ to deplete the cell of energy, halting phage replication Ilya Osterman now discovered that phages can rebuild NAD and overcome defense

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Sorek Lab

@SorekLab

5 years

Nice piece in Nature discussing our work on communication between phages: "The secret social lives of viruses"

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@SorekLab

2 years

Ever tried crossbreeding your phages? Check out our new study by @GarbJeremy : Phage mating helped us solve the mechanism of defense-associated sirtuins (DSR) – a large family of anti-phage defense systems

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Sorek Lab

@SorekLab

2 years

Anti-CBASS and anti-Pycsar! Our colleagues Sam Hobbs and Philip Kranzusch discovered phage-produced enzymes that inhibit bacterial immunity Phage enzymes degrade cyclic nucleotide signaling molecules Incredible biochemistry & structural biology feat!

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Sorek Lab

@SorekLab

4 months

Many phages are known to encode tRNAs of their own. Now Isaev, Wiedenhaft, Bikard and co show that these tRNAs enable phages to evade bacterial defense systems. A beautiful story, showing that the PARIS defense system cleaves host tRNAs when it detects infection. Congrats!

Artem Isaev

@Artem_Isaev_

4 months

Why do phages encode tRNAs? It aids in defeating tRNA-targeting immune systems. We provide structure and mechanism for the PARIS defense and show how it cleaves host tRNA upon trigger sensing. Stikingly, the non-cleavable tRNA of T5 supports the infection.

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Sorek Lab

@SorekLab

8 months

Out today @CellCellPress : a new family of immune proteins that deplete the cell of ATP and dATP when viral infection is recognized This immune mechanism is conserved from bacteria to animals Congratulations @FrancoisRousset !

François Rousset

@FrancoisRousset

8 months

Today, we report in @CellCellPress the discovery of a new mechanism of innate immunity encoded by diverse organisms : ATP cleavage ✂️ Thread below 👇

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Sorek Lab

@SorekLab

1 year

A hypothesis to explain why phages so frequently carry tRNAs of their own. Very interesting, makes a lot of sense

Stan Brouns

@StanBrouns

1 year

We just posted a preprint proposing a new role for the 50-year old conundrum of phage-encoded tRNAs: insensitivity to host tRNA depletion by anticodon nucleases (e.g. VapC). Great data analysis and hypothesis building by @DaanFvdBerg

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@SorekLab

1 year

A recording of my recent lecture at the NIH, summarizing our current understanding on the immune system of bacteria: new mechanisms, evolutionary links to the human immune system, how phages fight back, and open questions

The Immune System of Bacteria: Beyond CRISPR

WALS The arms race between bacteria and phages led to the development of sophisticated anti-phage defense systems, including CRISPR-Cas and restriction systems. We recently discovered that the...

videocast.nih.gov

NIH WALS

@NIHWALS

1 year

Live 1/25 at 2 p.m. ET, Rotem Sorek on immunity "beyond CRISPR" — #NIH lecture #RNA #CRISPR #Phage #immunity

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@SorekLab

1 year

Can't wait for the first Symposium on the Immune System of Bacteria, happening at the Weizmann Institute two weeks from now! Looking forward to welcoming you all and hearing of exciting new discoveries in the field!

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@SorekLab

3 months

Registration for the 2024 Boston Symposium on the Immune System of Bacteria is now open - an exciting program with a fantastic lineup of speakers! Additional talks will be selected from submitted abstracts

Sorek Lab

@SorekLab

8 months

I can't wait for the 2024 symposium on the "beyond CRISPR" immune system of bacteria, which will take place in Boston on April 16-18, 2024. Save the date!

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@SorekLab

1 year

New immune genes in humans are identified based on conservation with bacterial anti-phage defense systems! 🔥 Congrats @audeber and team for this important discovery!

MDMLab

@MDMlab_Paris

1 year

In a new preprint, we report that recently discovered anti-phages systems (Mokosh, Eleos, Lamassu…) have eukaryotic homologs. We harnessed this conservation to discover novel defense mechanisms in humans. @howmanyernest1 @gaofir @EnzoZ_P @AudeBer

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@SorekLab

2 years

Love this pic. The entire lab exploring and annotating new defense systems, in the "Defense 2.0 Blitz" week back in the summer of 2018. So many discoveries emerged from this session: CBASS, retrons, and now the recent addition of 21 new systems 🤩

Adi Millman

@AdiMillman

2 years

Since these were way too many to test we had to prioritize. This is where the whole Sorek lab joined the effort and in the first "Sorek lab hackathon", helped analyze and select systems for verification. 3/7

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@SorekLab

2 years

A bacterial immune system that specifically targets nucleus-forming phages. How cool!

A family of novel immune systems targets early infection of nucleus-forming jumbo phages

Jumbo bacteriophages of the ⌽KZ-like family are characterized by large genomes (>200 kb) and the remarkable ability to assemble a proteinaceous nucleus-like structure. The nucleus protects the phage...

www.biorxiv.org

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Sorek Lab

@SorekLab

1 year

A belated tweet: Our paper on how bacterial immune systems sense phage invaders was published last Friday @CellCellPress Talented Avigail Stokar-Avihail, who masterfully led this study, explains it in the thread below Congrats @AAvihail & coauthors!

Avigail Stokar-Avihail

@AAvihail

1 year

I am excited to share with you the final version of my work @SorekLab studying 💠How bacterial immune systems sense phage infection💠 Published now in @CellCellPress 🍻 See thread below for a recap and highlights added during the review process ⬇️ 1/11

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@SorekLab

6 years

The battle continues: DISARM, a widespread bacterial defence system w/ broad anti-phage activities not just #CRISPR

DISARM is a widespread bacterial defence system with broad anti-phage activities

Nature Microbiology - A widespread anti-phage defence system, DISARM (defence island system associated with restriction–modification), is identified.

www.nature.com

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@SorekLab

5 months

BiomX announces positive topline results from phase 1b/2a trial, testing phage cocktail against chronic infections in CF patients Some patients, who suffered from P. aeruginosa infection for >10 years, tested negative for the bug after phage treatment

BiomX Announces Positive Topline Results from Part 2 of the Phase 1b/2a Trial Evaluating BX004 for...

ir.biomx.com

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@SorekLab

6 years

Contemporary Phage Biology: From Classic Models to New Insights In depth review in @CellCellPress by @gaofir #phage

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@SorekLab

23 days

It is indeed an amazing anti phage defense system alright, but not CRISPR. This is a statue of Lamassu, the god protector of household in the Assyrian mythology. Lamassu is a family of defense systems present in ~10% of bacterial genomes, mechanism yet to be deciphered

Asaf Levy

@AsafLevyHUJI

23 days

crispr is amazing!! @OdedRechavi

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Sorek Lab

@SorekLab

2 years

Phages inhibit bacterial TIR signaling using "sponge" proteins Bonus: TIR-derived signaling molecule revealed! A super fun collaboration with the Kranzusch lab! Congrats Azita Leavitt, @ErezTzvi , @amitai_gil , Allen Lu!

Erez Tzvi Yirmiya

@ErezTzvi

2 years

Super excited to share our new preprint, discovering an anti-Thoeris protein and an immune signaling molecule produced by both plants and bacteria! A super fun and productive collaboration with the @SorekLab and the Kranzusch lab.

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@SorekLab

2 years

Out today: Bacteria, like humans, manipulate the cellular nucleotide pool to curb viral infection Originally posted in bioRxiv, now with new data @NatureMicrobiol Read the thread by Nitzan Tal, discoverer and leading author 💫

Bacteria deplete deoxynucleotides to defend against bacteriophage infection

Nature Microbiology - Depletion of the deoxynucleotide pool by a family of dCTP deaminases, and a family of dGTPases, defends bacteria against phage infection.

www.nature.com

Nitzan Tal

@Nitzan_T

2 years

Excited to share the final version of our story on 🌟 how bacteria use nucleotide depletion to fight viral infection🌟, now @NatureMicrobiol ! A brief recap, what was added, and some thoughts below 🦠🤩

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@SorekLab

1 year

Multiple new classes of phage proteins that inhibit bacterial immunity: Tad2 intercepts immune signals of defense TIR domains Gad1 traps the Gabija defense system in a cage And more... Fun collaborations with the Kranzusch lab!

T.LY URL Shortener URL Shortener, Custom Domain & Short Link Management

T.LY is the World's Shortest Free URL Shortener tool that takes a long URL and turns it into short links or QR codes that are easy to share and track the analytics. Create short branded URLs

t.ly

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@SorekLab

2 years

Two beautiful papers determine the structure of the anti-plasmid defense system Wadjet Structures explain how circular plasmid DNA is recognized and cleaved. Fascinating! Congrats @Gruber_Lausanne and @_KevinCorbett

GruberLab_Lausanne

@Gruber_Lausanne

2 years

Preprint🚨: Striking findings by @drhonsworth @RoisneHamelin_F and @DCI_EM showing how Wadjet SMC complexes (📏+✂️) eliminate smaller circular DNA (plasmids) in bacteria. Related work by @_KevinCorbett lab also out:

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@SorekLab

1 year

Beautiful visualization of evolution of phage and host!

Einat Tamar

@Einat_Tamar

1 year

E. coli and T7 phage migrate, interact, and diversify in a spatially structured environment: @RoyKishony @TechnionLive @BiologyTechnion

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@SorekLab

1 year

The Weizmann symposium on the Immune System of Bacteria: abstract submission deadline is next week, Jan 10th! Best abstracts will be selected for short talks We also have a few remaining slots for registration

Sorek Lab

@SorekLab

2 years

The first symposium on the "beyond CRISPR" immune system of bacteria: Registration open! Feb 28th-Mar 2nd at the Weizmann Institute, Israel A fantastic line up of speakers + talks selected from abstracts. We have limited space, hurry up and register!

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@SorekLab

1 year

Interesting: The BREX protein BrxL is not a protease, despite sequence and structure homologies to Lon proteases BREX was the first 'new' antiphage defense system discovered based on presence in defense islands, and its mechanism is still not understood

Nucleic Acids Res

@NAR_Open

1 year

Breakthrough**Structure, substrate binding and activity of a unique AAA+ protein: the BrxL phage restriction factor

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@SorekLab

2 years

Very interesting paper from the @edzewestra lab, showing that in conditions dictating longer phage replication cycles, cells are more likely to acquire CRISPR immunity. Relevant to many non-lab environments in which bacteria naturally grow slowly

Cell Host & Microbe

@cellhostmicrobe

2 years

Bacteriostatic antibiotics promote CRISPR-Cas immunity: @TatianaDimitriu @edzewestra find bacteriostatic #antibiotics promote #CRISPR -Cas immunity by slowing #phage replication. Delayed phage production allows cells to acquire spacers against phages

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@SorekLab

10 months

Finally, insight on the function of the Zorya immune system. A fascinating system sharing evolutionary origin with bacterial flagella. Have been waiting to learn more about this system for five years now, since we first reported it in our Doron 2018 paper

Alexander Harms

@AHarms485

10 months

Exciting talk by our friend and colleague Nicholas Taylor @NMITaylorLab on the Zorya anti-phage defense system - bacterial immunity is truly a gateway to molecular biology from the dark side of the moon!

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@SorekLab

2 years

Preprint: We studied defense islands in 1350 E coli genomes, involving >60 types of defense systems The vast majority of defense systems are carried on mobile genetic elements, that integrate in dedicated genomic positions Congrats @dinahoch !

Dina Hochhauser (@dinahoch.bsky.social)

@dinahoch

2 years

My turn! 🙋🏼‍♀️ Come read my FIRST first-author paper of my PhD @SorekLab , on the hot 🔥 topic of defence islands! 🏝 The defence island repertoire of the Escherichia coli pan-genome

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@SorekLab

9 months

Just learned that the glyco-cyclic ADPR immune signaling molecules we discovered, 2’ cADPR and 3’ cADPR, are available as purified products at Biolog: Very useful for researchers studying immune signaling in bacteria and plants

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@SorekLab

9 months

Beautiful depiction of our current knowledge on the bacterial immune system - recommended reading!

MDMLab

@MDMlab_Paris

9 months

So many novel antiphage systems out there! Hard to keep up with the pace of discoveries? We reviewed for @NatureRevMicro the highly diverse antiphage defence systems of bacteria; from molecular mechanisms to evolutionary drivers of such diversity.

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Sorek Lab

@SorekLab

6 years

Toxin-antitoxin database (type II recently updated ) How many TA pairs are in your favorite microbe? (probably not more than Microcystis aeruginosa)

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Sorek Lab

@SorekLab

6 years

Blurring the lines between plasmids and viruses A plasmid from an Antarctic haloarchaeon uses specialized membrane vesicles to disseminate and infect plasmid-free cells

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Sorek Lab

@SorekLab

11 months

In response to phage infection, bacterial gasdermins are activated to form membrane pores Here is a time lapse microscopy of cells infected by phage T6. Gasdermin in these cells is fused to GFP. Activated gasdermins aggregate & perforate the membrane (pink is propodium iodide)

Tanita Wein

@TanaWein

11 months

Studying the gasdermin system at the single-cell level in vivo we find that gasdermin is proteolytically cleaved during infection leading to gasdermin aggregation and membrane permeation.

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Sorek Lab

@SorekLab

7 months

A eukaryotic-like ubiquitination machinery in bacteria! Beautiful structures from the Corbett lab. This machinery defends bacteria against phage infection by targeting phage structural proteins and interfering with virion assembly

Kevin Corbett

@_KevinCorbett

7 months

I'm thrilled to share the latest work from my lab, showing that a bacterial anti-phage immune system encodes a ubiquitination pathway strikingly similar to our own! (1/12)

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Sorek Lab

@SorekLab

3 years

Gasdermins, important players in human immunity, are also present in bacteria As the human gasdermins, bacterial ones are activated by caspases to form membrane pores Congrats @TanaWein @algejohn ! Super fun collaboration with the Kranzusch lab Preprint:

Bacterial gasdermins reveal an ancient mechanism of cell death

Gasdermin proteins form large membrane pores in human cells that release immune cytokines and induce lytic cell death. Gasdermin pore formation is triggered by caspase-mediated cleavage during...

www.biorxiv.org

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Sorek Lab

@SorekLab

5 years

Look who’s talking! Discovery of numerous communication systems in a single bacterium-Paenibacilli are such social microbes! @MayaVoichek shares her story in our lab’s first #preprint Peptide-based quorum sensing systems in Paenibacillus polymyxa

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Sorek Lab

@SorekLab

3 years

A very important tool for anyone studying microbial genomes and phage-bacteria interactions!

Aude Bernheim

@AudeBer

3 years

A bit lost with all the new anti-phage systems out there? We developed DefenseFinder, a tool to detect all known anti-viral systems in bacterial genomes and conducted a large scale analysis on the anti-viral arsenal of prokaryotes. See more below ! (1/9)

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Sorek Lab

@SorekLab

10 months

A new immune signaling molecule, S-adenosyl methionine conjugated to AMP. Incredible!

Malcolm White

@mfwhite2

10 months

@HtChi221 After a lot of hard work with confusing and negative results, Haotian solved the puzzle and revealed a new signalling molecule, SAM-AMP, for antiviral defence. We hope you enjoy the paper!

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Sorek Lab

@SorekLab

2 years

Glad to be part of this important study!

ElinavLab

@Elinav_Lab

2 years

Proud to share our new work @CellCellPress . We identified and experimentally validated #microbiome strains contributing to IBD flareups across geography (4 countries), and developed a first-of-its kind combination phage therapy

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Sorek Lab

@SorekLab

8 months

Preprint: Human SARM1 can produce gADPR molecules as minor products SARM1 is a conserved TIR-domain protein known as an NAD-degrading enzyme. We now show that it can also produce signal molecules. This might be important in human neurobiology. A 🧵 1/9

The SARM1 TIR domain produces glycocyclic ADPR molecules as minor products

Sterile alpha and TIR motif-containing 1 (SARM1) is a protein involved in programmed death of injured axons. Following axon injury or a drug-induced insult, the TIR domain of SARM1 degrades the...

www.biorxiv.org

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Sorek Lab

@SorekLab

1 year

Type II Lamassu defense system (also called DdmABC in V. cholerae) is triggered by DNA stem-loop hairpin structures Structures are suggested to form during phage and plasmid replication from single-stranded or damaged DNA Interesting preprint from the Mekalanos lab!

bioRxiv Microbiology

@biorxiv_micrbio

1 year

Cell density-dependent death triggered by viral palindromic DNA sequences #biorxiv_micrbio

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Sorek Lab

@SorekLab

5 years

Exploring TASmania TASmania: A bacterial Toxin-Antitoxin Systems database (Hatice Akarsu et al) little genes, big impact

TASmania: A bacterial Toxin-Antitoxin Systems database

Author summary TASmania offers an extensive annotation of TA loci in a very large database of bacterial genomes, which represents a resource of crucial importance for the microbiology community....

journals.plos.org

0

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Sorek Lab

@SorekLab

1 year

Phage single-strand DNA binding (SSB) proteins are, in many cases, essential components of the phage replication apparatus. Many defense systems seem to sense the phage SSB as a signature of infection. Here is another example 👇

Cell Host & Microbe

@cellhostmicrobe

1 year

Hna confers #phage resistance. #Bacteriophage defense system “Hna” consisting of single predicted helicase/nuclease confers protection by abortive infection. Hna homologs present in array of #bacteria , including medically or agriculturally relevant species

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Sorek Lab

@SorekLab

6 years

Viruses cooperate to defeat bacteria (N&V) @AudeBer 's take on the fascinating phage interactions discovered by @AdairLBorges @joeBondyDenomy , @MCFRollins and M. Landsberger, S. van Houte @NatureNV #CRISPR

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Sorek Lab

@SorekLab

6 years

Phage isolation in the Sorek Lab @implosian #bioart #phage

1

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Sorek Lab

@SorekLab

3 years

Wow, this is a truly revolutionary method! Allows measuring gene expression in bacteria at the single cell level, and also records the position of the cell within the culture (e.g. biofilm). Congrats @Danidiin !! 💫

0

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Sorek Lab

@SorekLab

5 years

Ultrafast search of all deposited bacterial and viral genomic data

Nature Biotechnology - The global set of bacterial and viral sequences can be rapidly searched using a data structure inspired by web-search algorithms.

www.nature.com

0

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Sorek Lab

@SorekLab

3 years

If you ever wanted to know which collection of anti-phage defense systems your favorite bacteria encodes, PADLOC is the tool for you A nice analysis of defense systems distribution across microbes, plus discovery of some new ones!👇

Leighton Payne

@leighton_payne

3 years

🎉 Proud to share my 1st first-author paper! Introducing our new tool for defence system identification: PADLOC @NAR_Open 📰 (1/10)

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Sorek Lab

@SorekLab

5 years

Winner of the most beautiful plaque assay contamination is @gaofir who can beat that?

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Sorek Lab

@SorekLab

1 year

Cool study from the @joeBondyDenomy lab, showing how phages evade bacterial cGAS immunity using dedicated sponge proteins that sequester cGAMP Do viruses that infect humans use a similar mechanism to evade cGAS-STING? I bet they do Congrats @ErinHuiting and @joeBondyDenomy !

Erin Huiting

@ErinHuiting

1 year

Our latest CBASS story from the @joeBondyDenomy lab has arrived! 🙌Through a serendipitous collaboration with Yue Feng's lab, we uncovered an anti-CBASS protein that ‘sponges’ up a broad spectrum of cyclic dinucleotides. 1/14

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Sorek Lab

@SorekLab

6 years

Shake it to make it! Self-assembly phage shows how viruses assemble in nature, just add music! #3Dprinting #phage

Self-Assembly Phage

3D printed self-assembly phage, just shake!Music: Welcome Home by David Szesztay

www.youtube.com

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Sorek Lab

@SorekLab

2 years

Preprint: A giant protein complex drives anti-phage defense Multi-megadalton complex, formed by the RADAR system, deaminates adenosines into poisonous nucleotides during infection A wonderful collaboration with the Kranzusch lab, congrats @B_DuncanLowey and @Nitzan_T !

Brianna Duncan-Lowey

@B_DuncanLowey

2 years

I’m excited to share our preprint describing the structure of the RADAR antiphage defense system! This was such a fun collaboration with the wonderful @Nitzan_T and the @SorekLab ! A short thread about this huge machine (1/8)

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Sorek Lab

@SorekLab

6 years

Bacteria Use Collective Behavior to Generate Diverse Combat Strategies

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Sorek Lab

@SorekLab

8 months

Take a look at these beautiful electron microscopy images taken by Jens together with @ShaLGW . In these images, gold-labeled antibodies (black dots) mark the ubiquitin-like protein conjugated to the tip of the phage tail

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Sorek Lab

@SorekLab

24 days

François Rousset, a top-notch scientist in the exciting new field of microbial immunity, is opening his lab in Lyon. Highly recommended! ✨️🧫🔬

François Rousset

@FrancoisRousset

24 days

I am thrilled to announce that I will soon open my independent group at @CIRI_Lyon 🤩 My team will develop new approaches to explore the molecular arms race between phages and bacteria 🦠🧐 Join us in this new adventure in the beautiful city of Lyon !

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Sorek Lab

@SorekLab

7 months

Defense systems in Asgard archaea and the evolutionary path to eukaryotic innate immunity

bioRxiv Microbiology

@biorxiv_micrbio

7 months

Asgard archaea defense systems and their roles in the origin of immunity in eukaryotes #biorxiv_micrbio

1

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Sorek Lab

@SorekLab

2 years

An landmark study from the Polz lab explains how Defense Islands move between bacteria by mobile genetic elements, spreading phage-resistance traits

Martin Polz

@MartinPolz

2 years

Congratulations to Fatima Hussain @fatiays and @JavierDubert on their new paper in Science describing surprising ways how bacteria in the wild defend themselves against phage. See also perspective by Perspective by Meaden and Fineran.

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Sorek Lab

@SorekLab

2 years

Interesting implications for bacterial defense systems. "We demonstrate that intracellular defense systems are major determinants of phage host range and that overall phage resistance scales with the number of defense systems in the bacterial genome."

Franklin Nobrega

@NobregaFL

2 years

Fresh out of the oven! Panphage immunity in Pseudomonas by @AnaRitaMCosta , my former MSc student Daan van den Berg, and Jelger Esser from the @StanBrouns lab. Very happy to have been able to participate and see the impact of the @fagenbank in research.

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Sorek Lab

@SorekLab

6 years

Phage propagation for the holidays! #DeckTheLab @Addgene

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