Phillip Kyriakakis @breakliquid Twitter profile

Last Seen Profiles

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Phillip Kyriakakis

@breakliquid

4 months

Finally found a way to organize the -80!!! I drilled some holes in the racks and put key tags on them. No more using tape that falls off, now will have a database of the -80!

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Phillip Kyriakakis

@breakliquid

1 year

Bye bye $300 PCR machine from ebay. You lasted many years and helped clone hundreds of constructs. 🫡

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Phillip Kyriakakis

@breakliquid

1 year

Grammarly wanted to change "promoter" as in gene promoter to "champion". Should I keep it? LOL

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Phillip Kyriakakis

@breakliquid

1 year

So much GFP that is came out of solution!

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Phillip Kyriakakis

@breakliquid

4 months

My only goal for 2024 os to discover the function of the “vault”

Vault (organelle) - Wikipedia

en.m.wikipedia.org

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Phillip Kyriakakis

@breakliquid

2 years

My lab has arrived!!!

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Phillip Kyriakakis

@breakliquid

1 year

Does having a stop codon before a start codon prevent translation?

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Phillip Kyriakakis

@breakliquid

4 years

My students 3D printed some cool tube racks for the lab!

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Phillip Kyriakakis

@breakliquid

1 month

🤣 It made primetime:

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Phillip Kyriakakis

@breakliquid

6 months

New PCR machine, how to prevent this?

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Phillip Kyriakakis

@breakliquid

3 months

Fast in every way.

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Phillip Kyriakakis

@breakliquid

7 months

I think most everyone would pay postdocs way more if they could. The root problem is the flat NIH budget.

Ashley Ruba, PhD

@ashleyruba_phd

7 months

My PhD skills were extremely undervalued in academia: Sept 2019, post-doc: $50k/yr Sept 2020, post-doc: $52k/yr Sept 2021, post-doc: $54k/yr Sept 2022, UX researcher: $160k/yr Sept 2023, HF engineer: $150k/yr Forget #PostdocAppreciationWeek Just pay us more!

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Phillip Kyriakakis

@breakliquid

1 year

Wow!

Slava Bobrov

@slava__bobrov

1 year

This is what algae cell division looks like: #biology by W. Egmond

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Phillip Kyriakakis

@breakliquid

5 months

You're welcome!

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Phillip Kyriakakis

@breakliquid

2 years

I have a big announcement!

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Phillip Kyriakakis

@breakliquid

2 years

Inside a rapid “PCR” test (probably some isothermal amplification system).

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Phillip Kyriakakis

@breakliquid

2 years

But how do they fit all that shame in such a small package?

Azure Biosystems

@AzureBiosystems

2 years

Looking for a last-minute Halloween costume?

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Phillip Kyriakakis

@breakliquid

11 months

Wow, angstrom resolution 🤯

Ångström-resolution fluorescence microscopy

Nature - The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the...

www.nature.com

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Phillip Kyriakakis

@breakliquid

5 months

Just did a double take:

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Phillip Kyriakakis

@breakliquid

2 years

Its really happening!!!

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Phillip Kyriakakis

@breakliquid

1 year

@AlfredoAndere This must be a joke? Maybe it pays very very well, but that isn’t even in the ad 😂

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Phillip Kyriakakis

@breakliquid

6 years

Genetically Encoding PhyB Optogenetics - Phytobilin Production in Animal Cells

Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems...

Transplanting metabolic reactions from one species into another has many uses as a research tool with applications ranging from optogenetics to crop production. Ferredoxin (Fd), the enzyme that most...

pubs.acs.org

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Phillip Kyriakakis

@breakliquid

1 year

Ideas to convince young scientists to join science twitter?

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Phillip Kyriakakis

@breakliquid

1 year

@fasc1nate Where is the part where they smash your luggage?

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Phillip Kyriakakis

@breakliquid

1 year

Turned my neighbor’s broken fridge into a nice incubator! ~$80. Just used a thermo controller and heating pad.

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Phillip Kyriakakis

@breakliquid

11 months

Ooo, the engineering possibilities.

A single-domain green fluorescent protein catenane

Nature Communications - Natural proteins exhibit rich structural diversity based on the folds of an invariably linear chain. Here the authors design a single-domain GFP catenane as the counterpart...

www.nature.com

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Phillip Kyriakakis

@breakliquid

8 months

@duane_g_watson So, you will still make FedEx pay for a replacement, right?!

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Phillip Kyriakakis

@breakliquid

9 months

Why are RNA oligos so much more expensive than DNA?

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Phillip Kyriakakis

@breakliquid

3 years

So it's finally official! Moving to Stanford as Senior Research Scientist!!! @ the Wu Tsai Neurosciences Institute / Dept of Bioengineering

Wu Tsai Neurosciences Institute

neuroscience.stanford.edu

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Phillip Kyriakakis

@breakliquid

1 year

@hgupta84 @AcademicChatter The problem is you don’t always know how supportive they will or won’t be, but it is easy who has the most pubs or funding…

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Phillip Kyriakakis

@breakliquid

21 days

The nitroplast is the ______ of the cell.

🧬Jacob L Steenwyk

@jlsteenwyk

21 days

An advanced stage of symbiosis between a nitrogen-fixing bacterium and an alga is thought to capture the formation of a new type of organelle—a "nitroplast" #science #biology #symbiosis #evolution

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Phillip Kyriakakis

@breakliquid

10 months

@doctorveera @Nature Almost two years in revision, i wonder how much this paper evolved 😝

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Phillip Kyriakakis

@breakliquid

2 years

@roelverhaak @CellCellPress You make the government money flow to those journals when YOU choose to submit to those journals. You then make them more trendy, making others feel they need to publish there to get jobs and promotions. There are other options, let’s not do this black or white thinking.

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Phillip Kyriakakis

@breakliquid

2 years

Lots of progress for one day!

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Phillip Kyriakakis

@breakliquid

12 days

I can think of a lot of good reasons. But one stands out: Someone did it for me. And probably you.

Dalal Hamad

@DalalSci

13 days

Why do PIs take on undergraduates in their lab? Why would someone who has ongoing big projects decide, 'I’m going to take someone who is inexperienced in research and has a high chance of messing up, to help me with it'? Genuinely interested

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Phillip Kyriakakis

@breakliquid

4 months

One of my slides for tomorrow's lecture

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Phillip Kyriakakis

@breakliquid

2 years

My prototype red (650nm) led lamp for diaper changing at night.

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Phillip Kyriakakis

@breakliquid

1 year

@OGdukeneurosurg Wearing slippers?

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Phillip Kyriakakis

@breakliquid

1 year

I’m going to start a high impact journal/media: “Nature Tweets” It is the same thing as twitter except you pay $5 to read each tweet, your workplace has to subscribe, or the tweeter pays $100 to make it “free” for everyone. Sounds great, right? (And it has ads.)

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Phillip Kyriakakis

@breakliquid

2 years

Anyone observe lower plasmid yields in rich media (TB vs LB)?

A plasmid system with tunable copy number

Nature Communications - The range of available copy numbers for cloning vectors is largely restricted to the handful of ORIs that have been isolated from plasmids found in nature. Here the authors...

www.nature.com

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Phillip Kyriakakis

@breakliquid

3 years

@LappasiVenkat @KaushikLab Its the two days between PhD and postdoc.

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Phillip Kyriakakis

@breakliquid

2 years

OMG, after over a year, the hoods arrived!!! Now, how long until they are hooked up?!

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Phillip Kyriakakis

@breakliquid

7 months

why is it that nobody annotates the promoters on plasmids in Addgene?

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Phillip Kyriakakis

@breakliquid

8 months

All the mentors get this, but it is still pretty uplifting to receive!

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Phillip Kyriakakis

@breakliquid

1 year

My 3D printed charging station! Cost me $15 for the phone charger and that is it. The retail one (Belkin) is $150! It was a great motivator to learn 3D printing and designing stuff to print.

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Phillip Kyriakakis

@breakliquid

1 year

Great writing advice, especially for a dissertation/thesis, since it is often the first time someone writes such a long document. (From “Bird by bird”)

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Phillip Kyriakakis

@breakliquid

2 years

Any idea why these yeast colonies have this morphology?

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Phillip Kyriakakis

@breakliquid

1 year

Found a little dog roaming around campus today, seems mostly blind (cataracts). Any chance you are missing him?

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Phillip Kyriakakis

@breakliquid

6 months

Plasmid processing station, or lab toaster?

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Phillip Kyriakakis

@breakliquid

1 year

Uninvited dinner guest.

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Phillip Kyriakakis

@breakliquid

2 years

Lots of progress today!

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Phillip Kyriakakis

@breakliquid

4 months

I am also okay without someone else discovering it and me discovering it by reading about the discovery

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Phillip Kyriakakis

@breakliquid

2 years

@ChenxinLi2 @Kyle_Ireton It make you wonder if you ever used it with mold before it was visible…

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Phillip Kyriakakis

@breakliquid

1 year

Looks like a fun project.

Turns Out You Can Turn Old Blu-Ray Players into Microscopes

For those who need more resolution than an optical microscope offers, but on a hacker's budget.

gizmodo.com

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Phillip Kyriakakis

@breakliquid

1 year

Interesting cat whiskers Lalo has! Always dark near the face and white outside. Any idea how? I know hair color in cats can be temperature regulated. But isn’t the enzyme in the skin? How does this form?

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Phillip Kyriakakis

@breakliquid

2 years

Finished! Beat my previous time by over 5min too.

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Phillip Kyriakakis

@breakliquid

3 years

@Caroline_Bartma At Stanford we are told to keep the -80 at -70 to save energy (which also makes the -80 more reliable!). They tested a bunch of things and it was fine. Sorry I don’t have the reference.

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Phillip Kyriakakis

@breakliquid

1 year

1/x Check out our new preprint! Controlling neuronal connection tracing using optically modulated genes (PRT): 🧵👇

Light-Guided Rabies Virus Tracing for Neural Circuit Analysis

Neuronal tracing methods are essential tools to understand the fundamental architecture of neural circuits and their connection to the overall functional behavior of the brain. Viral vectors used to...

www.biorxiv.org

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Phillip Kyriakakis

@breakliquid

2 years

Congratulations @ATinyGreenCell on being invited as a keynote speaker at the Genome Writers Guild! You are being recognized along with David Baltimore, what a well deserved honor!

GWG 2022 Conference — Genome Writers Guild

www.genomewritersguild.org

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Phillip Kyriakakis

@breakliquid

2 years

How do people come up with this stuff?

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Phillip Kyriakakis

@breakliquid

1 year

Just uploaded my first preprint and i am so excited to share when it becomes available. Or should i wait until i have a tweetorial?

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Phillip Kyriakakis

@breakliquid

2 months

🤤 “Open-source microscope add-on for structured illumination microscopy”

Open-source microscope add-on for structured illumination microscopy

Nature Communications - Researchers developed an open-hardware structured illumination microscopy add-on. This affordable upgrade provides super-resolution capabilities for normal optical...

www.nature.com

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Phillip Kyriakakis

@breakliquid

1 year

How is it possible that when i miniprep these white colonies, all i have is a plasmid with LacZ!?!? When i restreak them, they are white colonies…

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Phillip Kyriakakis

@breakliquid

1 year

Rosemary is flowering! So much rosemary!

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Phillip Kyriakakis

@breakliquid

1 year

@CAS_mitolab Don’t worry, they will learn the hard way, we all do eventually 😂

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Phillip Kyriakakis

@breakliquid

1 year

I think this is a good exam question for undergraduate molbio...

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Phillip Kyriakakis

@breakliquid

3 years

1/ I'm excited to announce our latest #openaccess publication! Our lab designed an INEXPENSIVE LED system with DETAILED INSTRUCTIONS for non-engineers to (1) build the LEDs/circuit, (2) connect to a computer, and (3) run optogenetics experiments.

Building a Simple and Versatile Illumination System for Optogenetic Experiments (Video) | JoVE |...

Scientific Article | Watch this Scientific Journal Video about Building a Simple and Versatile Illumination System for Optogenetic Experiments at JoVE.com

www.jove.com

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Phillip Kyriakakis

@breakliquid

3 years

I am officially on the job market for a faculty job (assistant professor). Hoping this helps the Twitter algorithms send me more positions. Feel free to share them too!

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Phillip Kyriakakis

@breakliquid

1 year

@bertacarbene How does that happen and what are the consequences?

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Phillip Kyriakakis

@breakliquid

5 months

The best way to avoid integrating dna is to plan on it. When you want the dna to integrate, it doesn’t. 🤪

Phillip J. Buckhaults, Ph.D.

@P_J_Buckhaults

6 months

About 7% of cells transfected with linear pieces of DNA stably integrate said DNA into their genomes.

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Phillip Kyriakakis

@breakliquid

11 months

Intricate 3D architecture of a DNA mimic of GFP

Nature - X-ray crystallography and cryo-electron microscopy analyses of Lettuce—a DNA mimic of GFP—bound to various fluorophores reveal previously unknown structures of DNA that rival...

www.nature.com

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Phillip Kyriakakis

@breakliquid

3 months

@lukesjulson Love the “optical mouse” and random tiny people/scientists/postdocs?!

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Phillip Kyriakakis

@breakliquid

2 years

While the lab is far from functional, we christened the lab today by using e-power and an LED system from @ATinyGreenCell to cut out a gel band 🍾

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Phillip Kyriakakis

@breakliquid

1 year

😂 😂 😂 look at that hydrogen. It can “restructure water”! Hydrogen rich, get it now while it lasts.

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Phillip Kyriakakis

@breakliquid

2 years

Is this funny or scary?

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Phillip Kyriakakis

@breakliquid

1 year

A new devbio model?!?! (Glass frogs)

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Phillip Kyriakakis

@breakliquid

1 year

@wyomingwormboy @FeagaHeather I think many wet lab scientists think about becoming a baker as an answer to #3

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Phillip Kyriakakis

@breakliquid

1 year

For all those papers that put primer and gene sequences on a pdf 😂 😡

Akshay 🚀

@akshay_pachaar

1 year

Python is Powerful 🔥 Learn how to extract Tables from PDF & load it as a Pandas df 🐼 Check this out 👇

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Phillip Kyriakakis

@breakliquid

2 years

Today’s progress doesn’t look like much, but it was probably a lot. Mostly plumbing and electrical.

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Phillip Kyriakakis

@breakliquid

11 months

This 45 degree angle tube holder fits so perfectly! I made this to grow cells, can snap together.

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Phillip Kyriakakis

@breakliquid

1 year

@DennisEckmeier @GidMK @hubermanlab A researcher can’t cite a paper they never read and make claims about benefits and not take blame for the paper being fundamentally flawed. The whole point of trusting a researcher or expert is that they read AND digest the evidence objectively.

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Phillip Kyriakakis

@breakliquid

9 months

Just bought a used cytometer with no software. Any idea how to get it free/cheap? How to best/easy test the performance?

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Phillip Kyriakakis

@breakliquid

3 years

Chalongrat Noree (Charlie) in my PhD lab made this for me for my defense poster. Note: he is a photoshop master! I never had long hair, or blond hair! I’m so embarrassed about it, but it is such a great poster, so I’m posting it anyway!

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Phillip Kyriakakis

@breakliquid

2 years

After a few weeks under the bed, she is really taking to her new home ❤️

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Phillip Kyriakakis

@breakliquid

2 years

Finally got my replica plating tool!

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Phillip Kyriakakis

@breakliquid

2 years

Brought ice cream for a surprise for the whole building. Got the primo parking spot. Got double transformants grown over the weekend. It’s going to be a good week!

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Phillip Kyriakakis

@breakliquid

9 months

Problem solved:

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Phillip Kyriakakis

@breakliquid

11 months

Students last week thought you could only PCR 1kb at the most, and were plnning projects around that. Imagine that? How many things we all assume…

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Phillip Kyriakakis

@breakliquid

1 year

Beams of light through the window and doorway

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Phillip Kyriakakis

@breakliquid

2 years

@ATinyGreenCell That is why i never wanted to buy into the ecosystem (plus I couldn’t afford it for everyone in the lab). APE all the way! @ApEplasmid donate a little, use as you wish.

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Phillip Kyriakakis

@breakliquid

1 year

I suspect whole plasmid sequencing will have more impact than finishing the human genome. At least in the near term. It increases the pace of cloning, but also changes how i clone.

Vega Shah

@dr_alphalyrae

1 year

Method of the Year 2022: long-read sequencing | Nature Methods

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Phillip Kyriakakis

@breakliquid

2 years

@surt_lab I think using your name is appropriate in many cases. I wouldn’t want to tie my lab down to a specific theme or area, I am willing to go in whatever direction the science takes me. Over 10 or more years, it could change, as I have seen with many labs that choose themes.

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Phillip Kyriakakis

@breakliquid

7 months

What an amazing role model!

The day my supervisor won the Nobel prize in chemistry

Celebrations and support in the Bertozzi lab

www.chemistryworld.com

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Phillip Kyriakakis

@breakliquid

2 years

Great view of satellite 🛰 colonies

Mark O. Martin

@markowenmartin

2 years

One of my favorite things to show students: transform E. coli with pGLO on ampicillin plates. Incubate several days. Beautiful instruction with and without UV. Really teaches about antibiotic resistance, transformation, and medical issues. @univpugetsound @ASMicrobiology

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Phillip Kyriakakis

@breakliquid

1 year

Nice parts as well as tool!

A low-cost smartphone fluorescence microscope for research, life science education, and STEM...

Scientific Reports - A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach

www.nature.com

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Phillip Kyriakakis

@breakliquid

2 years

@DerekFFleming Maybe do it again in 2028?

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Phillip Kyriakakis

@breakliquid

2 years

Welcome our newest member of the family Lalo!

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Phillip Kyriakakis

@breakliquid

1 year

@ClementYChow @ricorox11 Now they need to scan all those papers and try and use a text reader to turn it into a text file for analysis. How will they do it in time?!?!

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Phillip Kyriakakis

@breakliquid

2 years

I want to see my miniprep next…