Spatial transcriptomics has emerged as a powerful tool for dissecting spatial cellular heterogeneity but as of today is largely limited to gene expression analysis. Yet, the life of RNA molecules is...

Nature - Spatial-ATAC-seq—spatially resolved chromatin accessibility profiling of tissue sections using next-generation sequencing—delineated tissue-region-specific epigenetic...

Spatial-CUT&Tag enables genome-wide tissue mapping of chromatin modification states at a cellular level.

DBiT-seq is a microfluidic-based method to deliver barcodes to the surface of a tissue slide to allow for spatial omics sequencing with 10-μm pixel size.

Cellular function in tissue is dependent upon the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial transcriptom...

The recent development of spatial omics methods enables single-cell profiling of the transcriptome and the 3D genome organization in a spatially resolved manner. Expanding the repertoire of spatial...

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It...

Spatial biology is emerging as a new frontier of biomedical research in development and disease, but currently limited to transcriptome and a panel of proteins. Here we present spatial epigenome...

Nature Methods - Recent technological advances in mass spectrometry promise to add single-cell proteomics to the biologist’s toolbox. Here we discuss the current status and what is needed for...

Nature Genetics - The field of spatial omics is developing rapidly, with a potentially transformative effect across many areas of biology. Nature Genetics invites authors to submit papers that use...

Current catalogs of regulatory sequences in the human genome are still incomplete and lack cell type resolution. To profile the activity of human gene regulatory elements in diverse cell types and...

TH2 functionality is indispensable in maintaining 5-year relapse-free durable remission in CAR T therapy.

On February 12, 2024, ISB is hosting a free one-day virtual workshop on Spatial Multiomics for Cancer Systems Biology

Nature Communications - Examining the spatially resolved transcriptome of tissue sections promises advances in biomedical research. Here, the authors present xDBiT, a versatile, microfluidics-based...

A new method maps the location of thousands of translating RNAs in cells and tissues

Mund et al. provide a perspective on single-cell proteomics and cell-type-resolved tissue proteomics and how they complement spatial transcriptomics. Together these technologies shed new light on...

RIBOmap enables single-cell in situ profiling of mRNA translation in 3D for thousands of genes in intact cells and tissues.

Nature - Our fifth annual round-up of the tools that look set to shake up science this year.

Formalin-fixed paraffin-embedded (FFPE) tissues are the most abundant archivable specimens in clinical tissue banks, but unfortunately incompatible with single-cell level transcriptome sequencing due...

Chimeric antigen receptor-modified (CAR) T cells targeting CD19 have mediated dramatic responses in relapsed/refractory acute lymphoblastic leukemia (ALL), yet a notable number of patients have...

The emerging field of single-cell proteomics is undergoing a phase of rapid technology development, including advances in mass spectrometry-based methods and ...

Nature - Smoothing the speed bumps in an important cellular pathway seems to be implicated in ageing.

Quantifying RNAs in their spatial context is crucial to understanding gene expression and regulation in complex tissues. In situ transcriptomic methods generate spatially resolved RNA profiles in...

Single cell omics has transformed the study of cellular heterogeneity, differentiation, and interactions at an unprecedented level. Our laboratory pioneered single-cell cytokine function profiling...

Single-cell analysis of primary and relapsed hepatocellular carcinoma tumors from patients reveal innate-like CD8+ T cells with low cytotoxicity and clonal expansion in the latter that may explain...

Nature Reviews Genetics - Spatial omics methods enable the charting of cellular heterogeneity, complex tissue architectures and dynamic changes during development and disease. The authors review...

The Richard W. Vague Professor in Immunotherapy in the Perelman School of Medicine is honored for pioneering the development of CAR T cell therapy, which programs patients’ own immune cells to fight...

Nature Methods - Multiomics sequencing goes spatial

Nature Aging - Epigenetic clocks can measure biological aging, but the relationship between epigenetic age and other hallmarks of aging is incompletely understood. Here the authors show that...

Spatial gene expression heterogeneity plays an essential role in a range of biological, physiological and pathological processes but it remains a scientific challenge to conduct high-spatial-resolu...