LoogerLab @LoogerL Twitter profile

Pinned Tweet

LoogerLab

1 year

Finally out! #jGCaMP8 #GCaMP indicators are super-fast with great SNR. Half-rise time 2ms. DNA & AAVs at @Addgene . Great collab w @svoboda314 @janeliagenie @ERSchreiter @SamWangPhD & many others! Best of luck! @HHMIJanelia @HHMINEWS @UCSDNeuro

Fast and sensitive GCaMP calcium indicators for imaging neural populations

Nature - Using large-scale screening and structure-guided mutagenesis, fast and sensitive GCaMP sensors are developed and optimized with improved kinetics without compromising sensitivity or...

www.nature.com

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Last Seen Profiles

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LoogerLab

@LoogerL

4 years

Hey Twitter, I realize that the world is burning and that very little is at all certain about how the coming months and years shake out. But I am excited to announce that the lab is moving to UC San Diego whenever it is safe & responsible to do so. We will be mostly in the 1/6

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LoogerLab

@LoogerL

5 months

Dear science world, I need help, please. If you like the tools and data we generate, and our shareware attitude, can you please co-apply for funding with us? It turns out that I'm terrible at writing grants, and we need $$ or else we can't make more GCaMPs, RCaMPs, 1/

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LoogerLab

@LoogerL

3 years

Yes! These ARE the GCaMPs you're looking for. Much faster, much better SNR, bright, no signs of toxicity (relative to current GCaMPs; we have not looked carefully yet). It's DEFINITELY worth upgrading your imaging from GCaMP6 or jGCaMP7 (or X-CaMP). Great collaboration!

Janelia GENIE

@janeliagenie

3 years

We are happy to release pre-publication the new jGCaMP8 series of Calcium indicators in led by Yan Zhang and Loren Looger ( @LoogerL )! The key advantage of these sensors is their fast kinetics (2-4x faster rise kinetics, up to 4x faster decay kinetics than jGCaMP7f)

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LoogerLab

@LoogerL

2 years

About the coolest application of #GCaMP and #iGluSnFR that I've ever seen!

Science girl

@gunsnrosesgirl3

2 years

How does a plant communicate danger signals? In response to a caterpillar bitting through its leaves, a wave of calcium travels across this plant to its furtherest parts , shown by fluorescent light. These signals may trigger defences mechanisms 1/2

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LoogerLab

@LoogerL

3 years

Hey tweeps let's play the game again where ppl list dream genetic-ish tools for neuroscience. Blue-sky level, plz. Sensors, effectors, tracers, probes, sequencing, viruses, promoters, EM, x-ray, cell atlases, anything! Janelia having brainstorm today & seeking crazy ideas

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LoogerLab

@LoogerL

4 years

Lab's 1st post: Our genetically encoded acetylcholine sensor, iAChSnFR, finally submitted & out on bioRxiv: . In vivo in mouse, fish, fly, worm. Constructs sent to @Addgene . Flies will be at @flystocks . Lots of awesome collaborators, 5 years in the making.

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LoogerLab

@LoogerL

3 years

Addgene is making AAV preps for the jGCaMP8 sensors! Add your serotype requests! (and tell me other promoter requests). Detailed info at and . Good luck!

jGCaMP8 Calcium indicators

The Looger Lab and the GENIE Project Team at HHMI Janelia have developed a new suite of jGCaMP8 calcium indicators, built on the GCaMP scaffold. The jGCaMP8 sensors have fast kinetics without

www.janelia.org

Addgene

@Addgene

3 years

New jGCaMP8 plasmids from @janeliagenie @LoogerL ! We're planning to produce AAVs for these starting next year with AAV1 and AAV9. If you'd find another serotype useful, add your suggestion here: 📷: @janeliagenie @LoogerL

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LoogerLab

@LoogerL

2 years

Yay! Such an awesome collaboration! @janeliagenie @svoboda314 @SamWangPhD @ERSchreiter @MishaAhrens @i__kolb @agetliang & many more not on Twitter. Cortex, cerebellum, fish, flies. Sensors are fast fast fast! High SNR, linear, work well in interneurons. They're at @Addgene !

bioRxiv Neuroscience

@biorxiv_neursci

2 years

Fast and sensitive GCaMP calcium indicators for imaging neural populations #biorxiv_neursci

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LoogerLab

@LoogerL

4 years

We will try our best to use the money wisely! Very excited!!!!

grobbins

@grobbins

4 years

UC San Diego hires 2 'star' brain scientists who will bring at least $20 million in research funding to campus. @sdut @ucsandiego @latimes @chronicle @ucsandiegoalumni @ResearchUCSD

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LoogerLab

@LoogerL

9 months

New jGCaMP8 viruses available @Addgene !! Get 'em while they're hot! #GCaMP #neuroscience #calcium

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LoogerLab

@LoogerL

6 months

Get your jGCaMP8 transgenic mice here!! #GCaMP

Janelia GENIE

@janeliagenie

6 months

Transgenic Tet- and Cre-inducible jGCaMP8m/s mice lines are now available as pre-order from JAX. Janelia is distributing a new RiboL1-tagged line that localizes jGCaMP8s to the soma. Check out the white paper describing all the lines and order here:

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LoogerLab

@LoogerL

3 years

New #GCaMP constructs alert! shipping to @Addgene syn1-H2B-jGCaMP8m/f/s CaMKII-8m/f/s mDlx-8m/f/s EF1a-8m/f/s gfaABC1D-Lck-8m/f/s Other fusions coming, too - more soon. Work from the amazing Yan Zhang & @janeliagenie

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LoogerLab

@LoogerL

1 year

awesome stuff! super-sensitive! everything available at @addgene of course! Sniff some Glu today!

Abhi Aggarwal

@abhi_aggarwal1

1 year

iGluSnFR3 is published! Check it out⬇️ We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission. This is #iGluSnFR3 in action detecting minis w/ TTX. @PodgorskiLab @HHMIJanelia @AllenInstitute

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LoogerLab

@LoogerL

2 years

ok huge batch of #jGCaMP8 stuff now available at @Addgene ! CaMKII promoter: 176750-2 mDlx (GABAergic) enhancer: 176753-5 EF1a promoter: 176756-8 gfaABC1D promoter (astrocytes): 176759-61 H2B fusion: 176748-9 axon-targeted already there: 172921-3 More on way! @janeliagenie

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LoogerLab

@LoogerL

11 months

Crazy stuff afoot here at @ucsandiego ! University has charged 67 students (20 of whom were not even there!) with "physical assault & endangerment" of Chancellor Khosla, even though videos clearly show it to be a peaceful protest. This seems like unnecessary escalation.

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LoogerLab

@LoogerL

4 years

Come see Prof. Akiko Iwasaki @VirusesImmunity “Immune response to SARS-CoV-2” today 2pm ET Zoom #443272306 . She & her lab have learned so much about #SARSCoV2 in such a short time! As always, recordings are available at

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LoogerLab

@LoogerL

3 years

#jGCaMP8 #iGluSnFR #iGABASnFR #AAVretro #CLEM #Eos5 #iAChSnFR #iSeroSnFR #neurotransmitter #imaging #CaMPARI #smFPs #tracing #RCaMP #RGECO #eMags #Magnets #optogenetics #synapse #spines #voltageimaging @HHMIJanelia @HHMINEWS all welcome! bring Qs & tool requests. #OpenScience

Optical Biology Seminars

@OBSeminars

3 years

Our next seminar is on Wednesday 17th February. We’re excited to be hearing from @LoogerL discussing tools to manipulate and monitor cellular activity

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LoogerLab

@LoogerL

4 years

Hey we are trying something different this week! Join @florian_krammer as he walks us through the myriad vaccine candidates progressing through trials world-wide. DNA, RNA, protein subunit, attenuated, killed, vaccine virus strains. How do safety & efficacy look so far? 1/

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LoogerLab

@LoogerL

3 years

hey trainees in #protein #engineering & #neuroscience ! We are planning the next iteration of the Junior Scientist Workshop on Protein Engineering: Making and Using Tools for Neuroscience and Other Biological Problems. Due to COVID-19, we are planning it to be virtual. 1/

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LoogerLab

@LoogerL

3 years

Tweeps I need advice. To address #ClimateEmergency , obviously we need tons of shit to happen. Lots of it is critical but not my cup of tea (e.g. advocacy, political wrangling.) What I'm good at is making & deploying new technology. Before you get scared, I'm not talking 1/

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LoogerLab

@LoogerL

11 months

I am very worried about AI. I am not worried in the way that most people seem to be worried right now. I worry that we're turning too much over to it already, and will turn much more over to it, and it is really unreliable. - When asked about science, it cites non-existent 1/n

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LoogerLab

@LoogerL

3 years

Super-excited for this! Will review all available tools for imaging, manipulation, tracing, etc. in neuroscience - not just our own. Will also cover how to figure out what's available, etc. - focusing on Open Science aspects of this field. Thanks for the invitation! 11am ET

[email protected]

@openneurosci

3 years

This Friday, Jun 18 at 15:00 UTC, join @LoogerL 's talk 'New tools for monitoring & manipulating cellular function' on our #opensource #neuroscience series via @worldwideneuro here:

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LoogerLab

@LoogerL

3 years

oh, definitely! let's see how GCaMP8 does, though... in general, though, it's not a competition. These are very different techniques with specific pros & cons. Very complementary.

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LoogerLab

@LoogerL

10 months

Major escalation here at @UCSanDiego : campus police ARRESTED 3 grad students on FELONY charges for writing on the sidewalk in chalk asking them to honor their side of the negotiations. Things are getting a little terrifying here - what's next?

UAW 4811

@uaw_4811

10 months

In an unconscionable move by the UC, University of California Police arrested 3 workers on felony charges and confiscated their possessions for writing messages in chalk and washable paint calling on UC to stop underpaying workers.🧵

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LoogerLab

@LoogerL

3 years

Here's the #optogenetics book chapter I promised. Hit me w/ Qs, comments, concerns, omissions, etc. Happy to stand corrected. Such powerful tools! Some concerns about how they're used these days #channelrhodopsin #neuroscience @GSegala @LorenaBenedet15

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LoogerLab

@LoogerL

3 years

Ah crap, did we forget to mention GCaMP47? Maybe you can still cancel your order. @janeliagenie @svoboda314 @SamWangPhD @MarthaBagnall @Addgene

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LoogerLab

@LoogerL

3 years

This is mindblowing

Slava Bobrov

@slava__bobrov

3 years

These bees remind me of the activation of neurons: #neuroscience

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LoogerLab

@LoogerL

4 years

1st in a series of posts asking researchers what they want/need to enable their science: What sensors are needed? New analytes, new modalities, improvements to existing reagents.

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LoogerLab

@LoogerL

5 months

I realize that almost all of my followers are GCaMPers, but another reminder that we're trying to work on climate stuff too. Would love to co-submit something on C capture, T tolerance, biofuel, plastic, anything.

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LoogerLab

@LoogerL

4 years

will start up. If you are interested, please DM or email me. Please RT and send to people that might be interested. UCSD has excellent resources, amazing collaborators, and it’s a beautiful place. 6/6

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LoogerLab

@LoogerL

2 years

#iGluSnFR is growing up!!!

Kaspar Podgorski

@PodgorskiLab

2 years

Check out our preprint on iGluSnFR3: Inspired by properties of endogenous glutamate receptors, we made an indicator that’s more sensitive and synapse-specific than previous generations. Recording by @fltomaska and @m8ta , @HHMIJanelia

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LoogerLab

@LoogerL

4 years

Wow @florian_krammer that was amazing!!!! Thanks for spending the time with us, and for all that you're doing in this fight. All, recording will be at: by tmr. Check out all the other great talks while you're there! #COVID__19 @S_Viswanathan1

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LoogerLab

@LoogerL

3 years

great series! @PavelTomancak Jan27, me Feb17, @KarlDeisseroth Mar3, @EmilianiLab Mar10, @FritjofHelmchen Mar17, @HillmanLab Mar24, Kate McDole Apr7, @SusanSuperRes Apr14, Tim Davies May5, @mhlangalab May12, @Tanner_Lab May19. Open to all, I think. Come ask questions!

Optical Biology Seminars

@OBSeminars

3 years

We are excited to launch our optical biology seminar series! We will be hearing from scientists in a range of different fields (development, cell biology, neuroscience) who are using optical tools to tackle biological questions. See our current line-up:

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LoogerLab

@LoogerL

4 years

We are really happy to welcome protein designer extraordinaire David Baker from UW @UWproteindesign tomorrow! Designing vaccines, drugs, detectors. Science in the Age of #COVID19 Zoom series from @HHMIJanelia . Every Thu 2pmET Zoom #443272306 . #COVID . with @S_Viswanathan1

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LoogerLab

@LoogerL

4 years

carbon capture and related environmental technologies. I am very open to new ideas & directions, so please bring them, even if they are very different from those above. We will be actively recruiting a number of positions, but because of COVID, I don’t yet know when things 5/6

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LoogerLab

@LoogerL

5 months

SnFRs (we've branched out to neuromodulators and neuropeptides), FPs, viruses, tags, etc. We'll also make whatever you want, but we need $$ to do it. DM or email me, sooner is better. Sorry to be desperate, but we want to keep doing this, and I'm not sure we can.

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LoogerLab

@LoogerL

4 years

Exciting news! Science/culture series from @HHMIJanelia @CSHL @UBARectorado @The_MRC @UCT_news @embl @NCBS_Bangalore @PKU1898 @Tsinghua_Uni . Life Science Across Globe: A Sister Institute Seminar Series. Weds >7/1 at 9amET-2pmUK-630pmIndia-9pmBeijing. All welcome. Will post link.

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LoogerLab

@LoogerL

3 years

woohoo! These are exactly the sorts of things we were hoping to hear from people! Of course, also send us complaints! Anyone having problems with them? New fusions & promoters sent to Addgene - should be ready to distribute in several weeks.

Dr. Heather Ratigan

@hcratigan

3 years

Just started 2p imaging with GCaMP8f in hippocampal dCA1 and WOW is it a pure upgrade from 6f. Blink and you miss it speed, gorgeous resolution, and not seeing overexpression even 5w post injection w/the syn promoter! Big ups to the whole dev team @janeliagenie @LoogerL

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@LoogerL

2 years

Hot, likely unpopular, take: If you're optimistic about politics, climate change, global public health, inequality, etc.... Then you're probably part of the problem.

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@LoogerL

4 years

Reminder: How are #vaccines trials going? Science in Age of #COVID19 with @florian_krammer THURSDAY Aug 6 2pmET Zoom 443272306. No password. Meeting size 3000. All welcome! Recorded. @S_Viswanathan1 @HHMIJanelia @HHMINEWS #VaccinesWork #vaccination #Corona

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@LoogerL

1 year

Very cool! They turned iGluSnFR into a very specific aspartate sensor (original is *fairly* specific, not *wildly* specific). Aspartate is of course a super-important metabolite (and a controversially proposed neurotransmitter). Congrats, @PLeippe & team!

Philipp Leippe

@PLeippe

1 year

🎉 Thrilled to share 4 yrs of hard work: iAspSnFR preprint at 🔬 GFP-based aspartate sensor, engineered via deep mutational scanning, tested in HEK293 cells. Highly specific for intracellular aspartate! #iAspSnFR #biosensor #aspartate

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@LoogerL

4 months

Amazing collaboration w/ @KeisukeYonehara & Akihiro Matsumoto. They got amazing imaging of GABA in amacrine cells w/ @janeliagenie 's iGABASnFR2. So exciting to see this sensor produce some fascinating biology.

Keisuke Yonehara

@KeisukeYonehara

4 months

I 'm very excited to share our new work! By 2p GABA imaging with new iGABASnFR2 sensor developed by @janeliagenie , we identified more than 40 functional GABAergic amacrine cell types in the mouse retina.

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@LoogerL

2 years

Come work with us at #UCSD ! Prior LM experience preferred but not required. Mix of science, ordering, organization, management, etc. #Neuro & #climate lab. We're fun to work with, laidback, super-collaborative. Great salary, benefits, access to UCSD perks. Plz RT!

jobRxiv

@jobRxiv

2 years

Laboratory Manager – Looger Lab Howard Hughes Medical Institute ( @HHMINEWS ), San Diego, USA #ScienceJobs

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@LoogerL

3 years

Hey everyone! Here's a recording of a fun talk that I gave yesterday for @OBSeminars . They asked great Qs! Very interactive. Log into Zoom at: Then password #40j8Rtp Enjoy! Tried to give shoutouts to all great collaborators!

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LoogerLab

@LoogerL

4 years

Hey all! Sorry, initial upload of @florian_krammer video was glitchy. Now fixed! Direct link to video: #COVID19 #vaccines #vaccination #VaccineTrials #coronavirus @HHMIJanelia @HHMINEWS Learn the latest about #SARSCoV2 vaccines from the world expert!

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LoogerLab

@LoogerL

3 years

Please come! Bring Qs, ideas for collabs, all the things!

The Cognitive Neuroscience of Development & Aging

@UNMC_CoNDA

3 years

Prof Loren Looger, PhD from @LoogerL @UCSD will speak on "New Tools for Monitoring & Manipulating Cellular Activity" @UNMC_CoNDA Center Seminar (Sept 17th at 12 PM CST). @UNMC_neurology @Creighton @UNLincoln @BoysTownHosp @UNMCCOM @UNLresearch @unmc_research @UNMC_HIV @HHMIJanelia

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LoogerLab

@LoogerL

4 years

Neurosciences department but plan an extremely multi-disciplinary, highly collaborative approach in multiple disciplines. Focus will, as before, be the construction, optimization, and dissemination of tools & datasets to accelerate biology - however, @ UCSD we will be able to 2/6

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@LoogerL

3 years

@Ulrike_Boehm @HHMIJanelia @HelenFarrants @ERSchreiter @HHMINEWS hey all! Anyone interested in #GCaMP should check out the data sheet () for the jGCaMP8 variants, which adds a little detail to the awesome talk on YouTube. They should be shipping from @Addgene verrry soon, hopefully this week! great collab w @janeliagenie

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LoogerLab

@LoogerL

4 years

pursue actual biological experiments within-lab much more than before. Topics will include, but aren’t limited to: protein engineering, fluorescent proteins, sensors, integrators, effectors, imaging technologies, electron microscopy, virus engineering (good kind), promoters, 3/6

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@LoogerL

4 years

Tomorrow, we welcome virologist Angela Rasmussen @angie_rasmussen at @Columbia . Host-pathogen interactions. Ebola guru. #Covid_19 sex diffs. Science in the Age of #COVID19 Zoom series from @HHMIJanelia @HHMINEWS Every Thu 2pmET Zoom #443272306 . with @S_Viswanathan1 Join us!

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@LoogerL

3 years

(canceled) PhD students & early post-docs. Plan is spring (April?). Let us know @ERSchreiter @rhodamine110 @LinTianLab @janeliaconf @agtebo please RT

Junior Scientist Workshop on Protein Engineering: Making and Using

Topics will cover aspects of modern protein engineering, particularly as it pertains to making tools for biology. Examples include genetically encoded sensors for molecules or cellular states;

www.janelia.org

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@LoogerL

3 years

This is only Bee-CaMP1, just wait for Bee-CaMP8

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LoogerLab

@LoogerL

2 years

Best GEVI currently on the market! But watch out @ERSchreiter @janeliagenie @Abdelfattah_Lab etc. have more tricks up their sleeves - other things headed down the pipeline!

Janelia GENIE

@janeliagenie

2 years

Introducing Voltron2, our chemigenetic voltage indicator, now with improved spike detection and sub-threshold sensitivity. Key mutation also enhances other Ace2 opsin-based sensors. Available on Addgene: #biorxiv_neursci

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@LoogerL

4 years

3rd in series: Please send successes & failures with retrograde labeling with AAV2-retro & other serotypes. We're screening for 2.0 variants with greater retrograde transport, particularly in tough cell types. Please provide: cells, Cre/straight, titer. Any prep.

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@LoogerL

1 year

Those 7 minutes are one of the best things ever to happen to me, both scientifically and personally. A healthy reminder that it's hard to confidently assign value to even straightforward-seeming events

Lauren Porter

@Lauren_L_Porter

1 year

For anyone feeling discouraged on the scientific career path, “The seven minutes that nearly killed my dream” was written for you. Grateful to @LoogerL and @HHMIJanelia for helping me to move forward.

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@LoogerL

3 years

Sensor available in lots of formats, several affinities, +/- fused redFP. Let us know if you want any modifications to it!

Daniel J Drucker

@DanielJDrucker

3 years

Development of novel flourescent glucose sensors for rapid, high-resolution live imaging of glucose influx, efflux, and metabolism in animals @CellReports

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@LoogerL

2 years

@HSchihada @UCSD_NIC @joachimgoedhart @LABrechaun @NathanShaner Having worked on FRET sensors for a long time, I can state that the current state-of-the-art with engineering distance & angle of two fluors is "who the fuck knows?"

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@LoogerL

3 years

Yay! This was such a fun collaboration! @ElizabethKUnger is a beast. This took a long time but well worth it. @LinTianLab #serotonin #imaging #machinelearning

Liz Unger, PhD

@ElizabethKUnger

3 years

I am beyond excited to finally be able to share this with the world: a genetically encoded, highly selective, large dynamic range SEROTONIN SENSOR.

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LoogerLab

@LoogerL

1 year

come learn about the latest in fluorescent proteins, sensors, imaging, etc. very interactive and open conference! @ERSchreiter @agtebo

Janelia Conferences

@janeliaconf

1 year

Join the seventh installment of @HHMIJanelia 's Fluorescent Proteins and Biological Sensors conference, showcasing the development of cutting-edge tools & technology for biological imaging. Learn more + apply by June 9 -> @ERSchreiter @agtebo @LoogerL

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@LoogerL

2 years

Now @Addgene is shipping AAV of our #glucose sensor #iGlucoSnFR : 164510 paper here:

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LoogerLab

@LoogerL

4 years

Tomorrow, we welcome Florian Krammer @florian_krammer at @IcahnMountSinai . Antibody responses to SARS2. Vaccine Jedi. Science in the Age of #COVID19 Zoom series from @HHMIJanelia . Every Thu 2pmET Zoom #443272306 . #COVID . with @S_Viswanathan1 . Join us!

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@LoogerL

1 year

Counterintuitive insight: I find it helpful to have multiple things that make me quite anxious. If things are going poorly in domain X, I can focus on domain Y. And if X&Y, then Z. I think this might be why a lot of ppl (including me) get passionate about sports. 1/n

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@LoogerL

3 years

@schoppik @MishaAhrens @HHMIJanelia @DiscoverMag it's also so old! I mean it's a badass video but my lord that's GCaMP5 FFS! only with jGCaMP8 can we *truly* know what goes through a fish's mind mwahahaha

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LoogerLab

@LoogerL

8 months

hey #protein #engineers who want to save the #climate ! so happy & proud to announce these grants! low barrier to entry! you don't have to be an expert to apply - just have a good idea. fast turnaround! $200,000. we'll give you lotsa feedback too. apply!!!

Homeworld Collective

@HomeworldBio

8 months

How can funding change a whole field? When it creates a cultural shift. We're thrilled to announce the launch of Homeworld's Garden Grants Program. First call: protein engineering toward sustainability goals. We co-built this for and with the climate biotech community 1/

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@LoogerL

3 years

Everyone, please let @Addgene know what the most helpful AAV serotypes are for your favorite constructs! If you don't tell them, they don't know what to produce. Please RT so that they can get large sample size.

Addgene

@Addgene

3 years

@LoogerL @jonvgill You can also use this form too @jonvgill !

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@LoogerL

3 years

#serotonin sensor #iSeroSnFR , co-developed with @LinTianLab @ElizabethKUnger , and its potential applications in research on #cognitive disorders such as #autism

HHMI | Janelia

@HHMIJanelia

3 years

This new sensor, co-developed by @LoogerL 's lab, can track serotonin activity in mice.

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LoogerLab

@LoogerL

5 months

Thank you for the enthusiastic replies so far! There's hope! Keep 'em coming! And apologies to everyone that has previously told me that we should apply - I mean well but I feel like I'm living Memento

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LoogerLab

@LoogerL

1 year

hi all, I guess many ppl doing this these days. #Twitter descending into abs hellhole. I need to take a break. If you want to reach me, please email. Lots of good ppl and science here, but the damage inflicted is too high. @elonmusk is profound threat to the planet. Stay safe all

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LoogerLab

@LoogerL

3 years

Y'all are all amazing! Amazing ideas emerged from this thread & the resulting discussion at Janelia. I'll put together a white paper on all the ideas and post it here. I haven't had a chance to go through the most recent ~100 ideas but will respond to each. Let's keep this going!

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LoogerLab

@LoogerL

4 years

Hey all! I did #VoteEarly in #Virginia and it was super-easy! Everything was freshly sanitized. The whole thing from parking to driving off took <10 minutes. For those near Janelia, early voting is at 750 Miller Dr SE, Leesburg -- GPS finds this easily. It's open M-F 8:30-5.

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LoogerLab

@LoogerL

3 years

Mini-rant: if you were/are genuinely surprised at any of: Jan. 6 insurrection, COVID resurgence, vaccinidiots, climate devastation & continual failure to act, etc. then you need to seriously reevaluate how you get your factbase, how you make decisions, if you are in a good place

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LoogerLab

@LoogerL

4 years

Great replies re sensors! Will reply with what we're working on, what's out there, etc. 2nd in series: What fluorescent proteins are needed? How are the various red, IR, blue FPs working for people?

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LoogerLab

@LoogerL

4 years

antibodies & other affinity reagents, virology, targeting & trafficking motifs, protein structure determination, mechanistic dissection of genetics of human disease, tools for non-model organisms, gene switches, genomics, etc. We will also pursue a very new direction: 4/6

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LoogerLab

@LoogerL

4 years

As a reminder, you can watch recorded talks in the @HHMIJanelia Science in the Age of #Covid_19 science seminar series at:

Panopto

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hhmi.hosted.panopto.com

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LoogerLab

@LoogerL

1 year

Finally! mGlyR found!!! #glycine

BryanRoth

@zenbrainest

1 year

WOW!

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LoogerLab

@LoogerL

4 years

Any red flags? Timeline for when we'll start to have more certainty? Challenge trials? Unknown unknowns? Bring your Qs! 2pmET Zoom #443272306 @S_Viswanathan1 @HHMIJanelia @HHMINEWS Science in Age of #COVID19 #vaccines #VaccinesWork #virus #Antibodies #vaccinations #Immunology 2/2

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LoogerLab

@LoogerL

3 years

@brendabl00dg00d @sthom003 oh this is just the tip of the iceberg. I review the field of optogenetics with associated problems, critical issues, and tools needed in a chapter due out in Principles of Molecular Imaging 2nd edition due out Aug. 2nd. Will post pdf. Yes we'll tackle it

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LoogerLab

@LoogerL

4 years

@JBorniger hi Jeremy whichever GCaMP variant you use (6, 7, 8 -- see other comments), I would definitely put it in the @LinTianLab axon-targeting format (GAP43 peptide). You can get this for 6 from @Addgene now. For 7, you could clone & make virus. For 8, you can get in a few weeks & clone

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LoogerLab

@LoogerL

4 years

@superkash @csaviochan @JBorniger hey Tom et al. we've sent them ( #jGCaMP8s , #jGCaMP8f , #jGCaMP8m ) off to @Addgene and they are fast-tracking them -- so hopefully 6-8 weeks. We're also trying to get a pre-print up on @biorxivpreprint So much to do...

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LoogerLab

@LoogerL

11 months

papers and non-existent phenomena, and vigorously defends its positions. It is writing legal briefs that cite non-existent cases. This is already permeating things at a pretty widespread range. Court cases will be adjudicated, and lost, based on crappy AI-written briefs. 2/

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LoogerLab

@LoogerL

4 years

We were really happy to welcome @timothysheahan , coronavirus expert from UNC, today! Another great lecture in the Science in the Age of #COVID19 Zoom series from @HHMIJanelia . Every Thu 2pmET Zoom #443272306 . Recordings available at

Panopto

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hhmi.hosted.panopto.com

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LoogerLab

@LoogerL

11 months

Please sign, retweet, share this faculty petition to drop bogus charges against peacefully protesting students here @UCSanDiego : This is a draconian overreach by an administration that is not honoring its side of the bargain.

WITHDRAW THE CHARGES

We write as faculty disturbed that 67 UCSD graduate workers have been charged in a student conduct process for exposing the campus' failure to maintain its wage obligations and core educational and...

docs.google.com

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LoogerLab

@LoogerL

5 months

Another important preprint about what #AlphaFold knows and doesn't know about protein folding, particularly fold switching, where a single sequence assumes two well-folded, very different structures. Check it out!

Lauren Porter

@Lauren_L_Porter

5 months

Our latest @bioRxiv manuscript reports an assessment of >500,000 AF2-based predictions of fold-switching proteins, which assume two distinct stable structures. These predictions fail to reflect observed energetics of fold switching 🧵

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LoogerLab

@LoogerL

2 years

Looking forward to this! Bring your questions, and a date if you want!

Manitoba Neuroscience Network

@manitobaneuro

2 years

Any plans for Valentine's day?! 😍 Spend your lunchtime with us on the @worldwideneuro ! We'll be hosting Dr. Loren Looger @UCSanDiego - New Tools for Monitoring and Manipulating Neural Circuits! See u Monday! @um_radyfhs @umanitoba @CAN_ACN @SiddiquiLab

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LoogerLab

@LoogerL

11 months

worry that it's still really fucking bad, and we're all somehow convinced that it's The Second Coming. Yes, someday it will be much better and maybe we need to worry about the Terminator, but right now I'm worried more that we're putting too much power & responsibility in 7/

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LoogerLab

@LoogerL

4 years

What are COVID research efforts with which we could assist? (testing in another post.) Are there any published sequences of neutralizing antibodies, for modeling, yet? I've seen some COVID SNP modeling. Would virus-SnFRs be useful? Could test how molecules affect propagation?

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LoogerLab

@LoogerL

4 years

Reminder to join Science in Age of #COVID19 every Thu at 2pm ET. Zoom 443272306. Recordings . Speakers so far: @VirusesImmunity Keith Jerome of UWVirology, @angie_rasmussen @florian_krammer @awyllie13 David Baker&Neil King of @UWproteindesign @KingLabIPD 1/

Panopto

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hhmi.hosted.panopto.com

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LoogerLab

@LoogerL

11 months

the hands of a bunch of Homer Simpsons, whom we mistake for a bunch of Sherlock Holmeses.

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LoogerLab

@LoogerL

7 months

YES! So proud! come look at the public "problem statements" of our 60+ applicants for Protein Engineering to Save the *** World. Some great stuff there! So proud of the @HomeworldBio team, @D_R_Goodwin @PaulReginato , and the applicants! 1/

Homeworld Collective

@HomeworldBio

7 months

🌱 Today is a special day for Homeworld: We are publishing over 50 proposals from Homeworld’s first Garden Grants program focused on protein engineering. 1/

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LoogerLab

@LoogerL

3 years

@neuralengine yeah, sorry I think 7 is old news. but don't worry - I don't believe that jGCaMP9 is on horizon. We will next focus on #RCaMP , #RGECO , other red/far-red GECIs, & red #SnFRs . 8 is the new 7. Red is the new green. New is the new new.

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LoogerLab

@LoogerL

2 years

Must see! #ClimateCrisis #Biotechnology #syntheticbiology #climatechange #microbes #carbon #microbiology @D_R_Goodwin @PaulReginato @sclarsic @orbuch @SexyLikeMeiosis @airminers @carbon_180 @aryelipman @tectonic @AdamMarblestone @heyjudka @thefreemanlab @klaus_lackner @ThatMrE

HHMI | Janelia

@HHMIJanelia

2 years

Tune in on May 4 to hear from Dianne Newman @caltech , Robert Finn @embl and Tobias Erb of the Max Planck Institute for Terrestrial Microbiology discuss “Learning from the planet to heal the planet: Microbial Ecosystems,” moderated by Ron Milo @MiloLabWIS

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LoogerLab

@LoogerL

3 years

Awesome collaboration studying #glutamate and #GABA transients in seizures!

Dimitri Kullmann

@Newrotrash

3 years

Our latest on glutamate and GABA release in interictal spiking and seizures | @VincentMagloire @yositeru8 @LoogerL

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@LoogerL

2 years

I speak Thursday 11:30ET @CreatesNy This will be for a diff audience than usual: #photonics #semiconductors #materials #fabrication #silicon #electrodes #AI #imaging I aim to deliver broad discussion of #NeuroTech in this space. Register at & bring Qs!

Dr. Loren Looger, University of California, San Diego, and HHMI - NY CREATES

Open-Source Nanophotonics Hardware & Reagents for Cutting-Edge Biology January 27 (Thursday), 2022

ny-creates.org

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LoogerLab

@LoogerL

2 years

hey team who all is using #jGCaMP8 (or earlier ones) in fiber #photometry ? If anyone has used various 8's, which one(s) would you recommend for ppl?

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LoogerLab

@LoogerL

4 years

Zoom 93793431497. First up, Gerry Rubin & Ruchi Parekh, & the Drosophila whole-brain connectome!

LoogerLab

@LoogerL

4 years

Exciting news! Science/culture series from @HHMIJanelia @CSHL @UBARectorado @The_MRC @UCT_news @embl @NCBS_Bangalore @PKU1898 @Tsinghua_Uni . Life Science Across Globe: A Sister Institute Seminar Series. Weds >7/1 at 9amET-2pmUK-630pmIndia-9pmBeijing. All welcome. Will post link.

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LoogerLab

@LoogerL

3 years

Happy new year! #Science in Age of #COVID will return this coming Tuesday at noon ET! Info & link coming soon. @S_Viswanathan1 @HHMINEWS @HHMIJanelia @angie_rasmussen @florian_krammer @EricTopol #antibody #vaccine #COVID20

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LoogerLab

@LoogerL

3 years

. @Twitter @TwitterSupport @instagram @Facebook you MUST do more to combat #vaccine disinformation. Yes, AntiVaxMomma's IG account came down after charges from NY DA. Was in plain sight for months! Many dangerous accts & posts still up. I'm embarrassed for you. Be better.

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LoogerLab

@LoogerL

2 years

re-upping this - awesome soma-jGCaMP8 plasmids & some cool results!

Hafting-Fyhn

@HaftingFyhnLAB

2 years

We have updated our preprint ““An updated suite of viral vectors for in vivo calcium imaging using local and retro-orbital injections”

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LoogerLab

@LoogerL

3 years

Lots of news on the #Science of #COVID19 front. First off, please join us next week, Tuesday 5th noonET for Jacob Lemieux w @sabeti_lab @broadinstitute for a discussion of poorly understood, transmission-increasing mutations in non-Spike genes. Bring your Qs! 1/

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