Rui Zhang @RuiZhangWUSTL Twitter profile | Pikagi

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Rui Zhang

@RuiZhangWUSTL

2,710

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2,114

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Associate Professor of Biochemistry at @WUSTLmed | #CryoEM studies of native #microtubules and #cilia related complexes lab website:

St Louis, MO

Joined July 2011

Don't wanna be here? Send us removal request.

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@RuiZhangWUSTL

Rui Zhang

2 months

Our foray to glycobiology and even TRP channel fields turned out to be a lot of fun😁 Great collaboration with @alanbrownhms , Karl Lechtreck and @DutcherLab

@alanbrownhms

Alan Brown

2 months

The mastigoneme, a little-known hair on algal cilia, contains some fascinating biology. Our latest work with @RuiZhangWUSTL reveals insights into the hydroxyproline glycosylation code, filament length control, and the coupling of filaments to ion channels

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@RuiZhangWUSTL

Rui Zhang

2 years

We are thrilled to share our latest work of the atomic structure of the central apparatus (CA) within the famous "9+2" architecture of motile #cilia .

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@RuiZhangWUSTL

Rui Zhang

3 years

We are very excited to share our latest work, the high-resolution cryo-EM structure of the ex vivo cortical #microtubules from human parasite #Toxoplasma gondii. This work is in collaboration with David Sibley lab and @alanbrownhms lab.

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@RuiZhangWUSTL

Rui Zhang

5 years

First paper from my lab after entering a new field. Great collaboration with @alanbrownhms and @DutcherLab ! In this paper we use high-resolution #cryoEM to identify 38 ciliary doublet associated proteins including 33 #microtubule inner proteins ( #MIPs ) 😎

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Structure of the Decorated Ciliary Doublet Microtubule

Visualizing axonemal microtubules and the proteins that decorate them, on the outside and inside, points to how the underlying periodic architecture supports cilia function.

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@RuiZhangWUSTL

Rui Zhang

4 years

N.O.A. 🥰 In the next 5 yrs, I will have money to study the mysterious functions of MIPs inside ciliary doublet microtubules, using chlamydomonas mutants and cryoEM. Stay Tuned.

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@RuiZhangWUSTL

Rui Zhang

2 years

We are thrilled to have Eva visiting WashU and my lab! 🥳🤩

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@RuiZhangWUSTL

Rui Zhang

5 months

My academic activities in 2023: - Submitted a multi-PI R01 grant and got 2% score - Published one Cell paper, one NC paper - Attended two conferences in person (first since the pandemic) - Got my tenure approved by the ad hoc committee Hopefully 2024 will be even better 🤟

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@RuiZhangWUSTL

Rui Zhang

2 years

NPC is NOT "the largest molecular machine in human cells". The system we work on, the axoneme within the cilia, for example, has a diameter of ~220 nm with more than >500 distinct protein components. In comparison, NPC has a diameter of ~130 nm and <50 distinct proteins.

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@Nature

nature

2 years

What's next for AlphaFold and the AI protein-folding revolution

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@RuiZhangWUSTL

Rui Zhang

1 year

We are thrilled to share our latest structures of doublet microtubule from sea urchin sperm and bovine sperm. This is a wonderful collaboration with Tzviya Zeev-Ben-Mordehai @TzviyaZBM , Alan Brown @alanbrownhms , and Susan Dutcher @DutcherLab .

@TzviyaZBM

Tzviya Zeev-Ben-Mordehai

1 year

I am thrilled our results on sperm axonemal doublet #microtubules are now out in Cell. Outstanding work of Miguel Leung and amazing collaboration with @RuiZhangWUSTL and @alanbrownhms

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@RuiZhangWUSTL

Rui Zhang

3 years

Please RT: One postdoctoral position is available in my lab () @WUSTLmed to study the structures and functions of native macromolecular complexes related to #microtubule #cilia and @cytoskeleton from various species including #parasites and human.

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@RuiZhangWUSTL

Rui Zhang

3 years

This is one of my all-time favorite articles by @sami_chaaban & @GaryBrouhard : If I can get the money, I would love to solve structures of the #microtubule in Fig. 4 and 7! Working with mosquitoes should fall into the 'high-risk-high-reward' category 🤔

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@RuiZhangWUSTL

Rui Zhang

3 years

Another wonderful collaboration with @alanbrownhms and @DutcherLab . Here we put complementary data together to build the complete structure of two distinct radial spokes on doublet microtubule. It was also a great experience to coordinate with the Vale lab for paper submission.

@alanbrownhms

Alan Brown

3 years

It always amazes me the things that can bind microtubules - our structures of radial spokes bound to ciliary doublet microtubules

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@RuiZhangWUSTL

Rui Zhang

3 years

Hi, we are still actively looking for postdoc(s). Candidates with structural biology background or passion for microtubule/cilia are preferred. Please retweet.

@RuiZhangWUSTL

Rui Zhang

3 years

Please RT: One postdoctoral position is available in my lab () @WUSTLmed to study the structures and functions of native macromolecular complexes related to #microtubule #cilia and @cytoskeleton from various species including #parasites and human.

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@RuiZhangWUSTL

Rui Zhang

3 years

assemble heterotrimer at home👇

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@RuiZhangWUSTL

Rui Zhang

2 years

Yes, the Zhang brothers will both be presenting the same atomic structure of the central apparatus (pair) from Chlamydomonas, but with different focuses😁 Science won't get more exciting than that 🤩

@Mill_lab

The Mill Lab

2 years

We have 6 sensational speakers talking about the latest in #cilia and #centrosome science from around the world! The Hsai lab (🇹🇼), @SteibEmmanuelle ( @VermotLab ), @scott_wad_dell ( @boulter_lab , @Mill_lab ), the Cardoso lab (Columbia), @RuiZhangWUSTL AND @zhang_lab ! 🔥🔥🔥

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@RuiZhangWUSTL

Rui Zhang

11 months

Another groundbreaking study by the @GaiaPigino lab! As the authors speculated (and in our 2019 paper as well), MIPs could recruit different tubulin modifying enzymes to specific PFs. In fact, MIP FAP90 has half of its length lies at the external surface, between PF B08 and B09!

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@biorxivpreprint

bioRxiv

@biorxivpreprint

1 year

The Tubulin Nano-Code: a protofilament-specific pattern of tubulin post-translational modifications regulates ciliary beating mechanics #bioRxiv

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@RuiZhangWUSTL

Rui Zhang

2 years

Where the structural biology is heading?

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@RuiZhangWUSTL

Rui Zhang

3 years

Want to buy a book to read during the lockdown? I highly recommend this one 👇😁

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@RuiZhangWUSTL

Rui Zhang

3 years

I know all the authors 😁 and contributed one figure 👇

@RubinsteinJohn

John Rubinstein

@RubinsteinJohn

3 years

Delighted that Bob Glaeser's, @NogalesLab 's, & @chiulab 's new textbook on cryoEM is now published - with a lovely dedication to Ken Downing. Highly educational chapters from many experts from the cryoEM community. Sure to become a classic - but hopefully out of date soon!

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@RuiZhangWUSTL

Rui Zhang

4 years

This work from @GaiaPigino lab is absolutely beautiful! Structures of primary cilia and motile cilia are very different

The molecular structure of mammalian primary cilia revealed by cryo-electron tomography

Nature Structural & Molecular Biology - Primary cilia are isolated from monolayers of mammalian cells in culture for cryo-ET analyses, revealing new features as well as how they differ from...

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@RuiZhangWUSTL

Rui Zhang

3 years

I am excited to share this manuscript 👇 GTP-microtubule lattice is expanded 😎

@biorxiv_biophys

bioRxiv Biophysics

@biorxiv_biophys

3 years

Structural transitions in the GTP cap visualized by cryo-EM of catalytically inactive microtubules #biorxiv_biophys

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@RuiZhangWUSTL

Rui Zhang

4 years

Very excited about the coming #bps20 ! I will be giving a 15-mins talk titled "high-resolution #cryoem structure of a natively decorated doublet #microtubule " at the Saturday #motility & #cytoskeleton subgroup.

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@RuiZhangWUSTL

Rui Zhang

10 months

Thank you @KatCiazynska so much for highlighting our recent work with @TzviyaZBM @alanbrownhms @DutcherLab labs!

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Structural organization of the sperm tail

Nature Structural & Molecular Biology - Structural organization of the sperm tail

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@RuiZhangWUSTL

Rui Zhang

2 years

One of the top five cryo-EM labs to join 👇

@RubinsteinJohn

John Rubinstein

@RubinsteinJohn

2 years

Our laboratory has postdoctoral position(s) available to work on cryo-EM of large and dynamic membrane protein complexes. Cryo-EM experience is not required. Please RT!

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@RuiZhangWUSTL

Rui Zhang

3 months

Preparing a talk at my alma mater Baylor College of Medicine this Thursday. I dig out an old photo on my thesis defense day in 2010. As a graduate student I collaborated with scholars at Washington University in St. Louis, which is my home now. #nostalgia #sichuan_food

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@RuiZhangWUSTL

Rui Zhang

4 years

Sparsely decorated microtubule associated protein

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@RuiZhangWUSTL

Rui Zhang

2 years

Current status of my lab (1 PI, 2 postdocs, and 1 roton)

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@RuiZhangWUSTL

Rui Zhang

3 years

This utilizes the full power of cryo-EM 👇 I wish more and more structural labs are doing this kind of approach, even though it is not "hypothesis driven" 🤪

@naturemethods

Nature Methods

3 years

The iterative “Build and Retrieve” (BaR) methodology from Edward Yu from @CWRUSOM and colleagues facilitates solving cryo-EM structures of multiple membrane (and soluble) proteins simultaneously.

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@RuiZhangWUSTL

Rui Zhang

6 years

Previously we managed to obtain the first atomic structure of microtubule with the help of markers for tubulin dimer (kinesin, EB3, TPX2...), now we can do it without the markers! Separating the effects of nucleotide and EB binding on microtubule structure

Separating the effects of nucleotide and EB binding on microtubule structure | Proceedings of the...

Microtubules (MTs) are polymers assembled from αβ-tubulin heterodimers that display the hallmark behavior of dynamic instability. MT dynamics are d...

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@RuiZhangWUSTL

Rui Zhang

3 years

Please see a dispatch that @alanbrownhms and I wrote about the recent publication of the beautiful cryo-ET structure of primary cilia from @GaiaPigino group.

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@RuiZhangWUSTL

Rui Zhang

4 years

Changing cover letters for different journals

@OdedRechavi

Oded Rechavi

4 years

Francis Picabia’s art

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@RuiZhangWUSTL

Rui Zhang

8 months

Here is my Google Scholar Word Cloud. Are you surprised 😁?

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@RuiZhangWUSTL

Rui Zhang

2 years

I really need to improve my presentation skills ...

@LecuitLab

TLecuitLab

2 years

A perspective on Motor driven actin flow by Rob Phillips…

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@RuiZhangWUSTL

Rui Zhang

3 years

Dear all, I forgot to update the test results: using similar number of particles, the ones from cubic ice and vitreous ice produce 3D reconstructions with similar resolution/quality.

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@RuiZhangWUSTL

Rui Zhang

2 years

I always wanted to try BioRxiv, but for every paper we always have at least one prominent group directly competing on the same structure. Seems preprinting is a bit risky for structural biology when the paper is about identification of new proteins. Correct me if I am wrong 🙏

@PracheeAC

Prachee Avasthi

2 years

Settle an argument for me: What prevents a greater proportion of scientists from preprinting? Lack of info, fear/distaste of sharing work prior to peer review, perceived lack of benefit/reward/credit, what else? Muting replies so I can work, but thanks in advance! 🙏

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@RuiZhangWUSTL

Rui Zhang

7 years

Our TPX2 paper is finally out in @eLife IMO a big step towards our understanding of the mysterious process of microtubule nucleation. From 0 to 1 is always a big deal!

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Structural insight into TPX2-stimulated microtubule assembly

A combination of cryo-electron microscopy of TPX2 bound to microtubules and in vitro reconstitution experiments reveals a novel microtubule interaction mode that explains how TPX2 promotes microtub...

elifesciences.org

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@RuiZhangWUSTL

Rui Zhang

2 years

Doesn't matter where it is published, doesn't matter what is the so called "biological significance", this is one of my favorite structures of ALL TIME👇

@OliBClarke

Oli B. Clarke

2 years

1/10 The ankyrin complex story from our lab @ColumbiaPS ⬇️ is out now in @NatureSMB , along with a beautiful study by @zhou_lab and news & views piece by @AshToye_Bristol . Congrats to all authors & particularly @Frances84469032 ! Read on for developments since the preprint:

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@RuiZhangWUSTL

Rui Zhang

6 months

Happy to contribute a cryo-EM structure of HURP decorated microtubule that helps explain how HURP stabilizes the microtubule.

@LabPetry

Sabine Petry

6 months

Just when you think you have it all, Venecia Valdez and team from @RuiZhangWUSTL and @LabPetry prove us wrong: HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2

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@RuiZhangWUSTL

Rui Zhang

3 years

A great achievement in cilia field towards the understanding and treatment of human primary ciliary dyskinesia (PCD) 👏 And remarkable evolutionary conservation between the green algae and human! Congratulations to the team! 🥳

@alanbrownhms

Alan Brown

3 years

We used #cryoEM to identify the proteins that form mammalian doublet microtubules in bovine respiratory cilia and contribute to the motility of cilia @miao_gui @jacobranderso @JosephineBotsch

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@RuiZhangWUSTL

Rui Zhang

2 years

A great review that single-handedly boosts my h-index 😍

@JoeAthertonKCL

Joe Atherton

@JoeAthertonKCL

2 years

A mini-review with James Garnett for @FrontMolBioSci discusses EM & ssNMR developments in the structural biology of filaments over the last decade or so, focussing on microtubules and pili. Amazing how much these fields have progressed! Open access here;

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@RuiZhangWUSTL

Rui Zhang

4 years

🥳 Where is your hugging-the-box picture?

@QB3Berkeley

QB3-Berkeley

4 years

It's here: QB3-Berkeley, @NogalesLab , and @TheHurleyLab have welcomed a @thermofisher Krios G3i Cryo-TEM to Stanley Hall! 🎉 This new instrument will help increase the @UCBerkeley research community's access to cryo-electron microscopy 🥼🔬 🥳

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@RuiZhangWUSTL

Rui Zhang

2 years

Congratulations to the team! I have to confess that the Abeta42 fibril structure we determined in 2009 (fibrils grown at PH 2😅) looks nothing like the native structure 👇 but we are not alone 😂 This is exactly the reason we all should go native!

@SjorsScheres

Sjors Scheres

2 years

Now out in @ScienceMagazine : #cryoEM structures of #Amyloidbeta42 filaments from human brains. By @yyang_cella , @DianaArseni & @wjzhwed , with @bwfalcon and @MichelGoed ! 😍 #NoClouds

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@RuiZhangWUSTL

Rui Zhang

4 years

I and my startup

@OdedRechavi

Oded Rechavi

4 years

You and your opinion

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@RuiZhangWUSTL

Rui Zhang

6 months

One nice thing about attending conference in person is that you got to take picture with the microtubule giant 😊

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@RuiZhangWUSTL

Rui Zhang

3 years

CryoEM folks, I wonder if anyone has tested that the samples within the cubic ice are really damaged and fail to contribute to 3D reconstruction?

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@RuiZhangWUSTL

Rui Zhang

1 year

Beautiful structure from native sources! 😍

@CellCellPress

Cell

1 year

Now online! Structural basis of membrane skeleton organization in red blood cells

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@RuiZhangWUSTL

Rui Zhang

3 years

We respectfully request a recount of the votes 🤪

@jbiolchem

Journal of Biological Chemistry

3 years

It all comes down to this! #TeamMassSpec gets a shot at the title against the champ #TeamCryo in the #JBCMethodsMadness Championship! FINAL ROUND: #TeamMassSpec vs. #TeamCryo Who should advance to the next round? Let us know in the comments and retweet to help spread the word!

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@RuiZhangWUSTL

Rui Zhang

4 years

Thanks to the modern technology, we still got to hear Sunney Xie’s wonderful talk at #bps20 @BiophysicalSoc , and learn his heroic efforts in fighting #nCoV2019

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@RuiZhangWUSTL

Rui Zhang

4 years

Who doesn’t like #cilia

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@RuiZhangWUSTL

Rui Zhang

2 years

"CKAP2 increases the apparent rate constant Ka of microtubule growth by 50-fold and increases microtubule growth rates...Our results identify CKAP2 as the most potent microtubule growth factor to date."

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The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules

Demonstration of the biochemical capacity of the spindle protein CKAP2 as the most potent assembly factor of microtubules.

elifesciences.org

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@RuiZhangWUSTL

Rui Zhang

4 years

I just placed the order 👇can't wait to get back to work/life 🥳

@EvanKirstel

🇺🇦Evan Kirstel #B2B #TechFluencer

4 years

Ok I laughed out loud. If you’re not laughing, you’re crying right? #CoronavirusOutbreak #coronapocolypse

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@RuiZhangWUSTL

Rui Zhang

3 years

This is just the beginning...

@biorxivpreprint

bioRxiv

@biorxivpreprint

3 years

Structures of core eukaryotic protein complexes #bioRxiv

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@RuiZhangWUSTL

Rui Zhang

3 years

Hear EHS just walked in the neighboring lab 👇

@OdedRechavi

Oded Rechavi

3 years

“Revision has been requested and a new version is required within two months.”

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@RuiZhangWUSTL

Rui Zhang

4 years

auto sharpening

@OdedRechavi

Oded Rechavi

4 years

The first computer, all the way down, was a turtle

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@RuiZhangWUSTL

Rui Zhang

4 years

I've spent the whole day watching the pre-recorded videos of 2020 EMBL/EMBO #microtubule symposia #EESMTs . Great format! 🤩😍

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@RuiZhangWUSTL

Rui Zhang

3 years

Ready for FIB-Milling 👇

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@RuiZhangWUSTL

Rui Zhang

2 years

Please also see a News & Views by Elena Zehr & Antonina Roll-Mecak

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@RuiZhangWUSTL

Rui Zhang

3 years

Believe or not, we only get to see the other paper today. Beautiful structure and functional data! It is great to see different approaches reached the same protein assignment 🥳

@HahamIris

Iris Grossman-Haham

3 years

In my first tweet, I'm excited to share my work on reconstituting the radial spoke head - 9 proteins! Thank you, @BhabhaEkiertLab , @ZanlinCryo and @halfbuddha for making 3.1 A possible!

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@RuiZhangWUSTL

Rui Zhang

2 years

Snapshots of actin and tubulin folding inside the TRiC chaperonin 🤩

@JuhaHuiskonen

Juha Huiskonen

2 years

1/ How do proteins take their shape in the cell? Insights from our article with @JackJKelly @TheYueLab @UniofOxford @v_paavilainen @HiLIFE_Helsinki @LHapponen @lunduniversity @structbiolbxl @imperialcollege @pfizer #OpenAccess 🧵👇

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@RuiZhangWUSTL

Rui Zhang

6 months

Hello #cellbio2023 so great to be back in person! I look forward to reconnecting with old friends and meeting new friends! My postdoc Jianwei Zeng will give a talk on sperm flagella structure on Dec 5th 5:20pm.

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@RuiZhangWUSTL

Rui Zhang

4 years

This is very impressive work! Congratulations, Kai! It is amazing to see how high-res single particle cryo-EM has advanced the motile cilia field in the past few years! Of course, none of these can be easily designed/interpreted without the knowledge from previous work by cryo-ET

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@RuiZhangWUSTL

Rui Zhang

1 year

Folks at @WUSM_BMB , no more wondering about the suspicious fish tank with edible purple sea urchins (that I brought from a restaurant in Monterey CA).

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@RuiZhangWUSTL

Rui Zhang

2 years

@PiperProteins We thought the rendering by ChimeraX is pretty good, but #Blender3D brings the visual effect to another level 🤩 Thank you so much!

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@RuiZhangWUSTL

Rui Zhang

4 years

I have a single particle story to tell, but it is not polished yet

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@RuiZhangWUSTL

Rui Zhang

6 years

Glad that I contributed to this great paper! Lots of data!

@JCellBiol

JCellBiol

6 years

Augmin is required for the nucleation of new microtubules from preexisting MTs. Song et al @PrincetonMolBio show that augmin functions as a direct bridge between the preexisting MT and the γ-tubulin ring nucleation complex that forms the base of the new MT

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Rui Zhang

2 years

Finally, a special figure panel for #microtubule lovers. One of the MIPs FAP225 is unhappy about the original tubulin lateral interface and decides to substitute part of the H1'-S2 loop and form a enhanced interface 🤩

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@RuiZhangWUSTL

Rui Zhang

5 years

@builab Sorry about that😜 You work have been a great inspiration for us! I think your recent cryo-EM map is at similar quality, the only missing factor is @alanbrownhms 😁

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@RuiZhangWUSTL

Rui Zhang

3 years

@bengeliscious @ReiterLab @CentrioleLab @Yoshi__Ichikawa @StearnsLab @GaiaPigino @PracheeAC @WallaceUcsf @LottePedersen12 @Mill_lab @Carter_Lab @David_S_Bristol @alanbrownhms @BastinLab_Paris @esben_lorentzen Here is what I got. I joined Tb IFT-74 to Tb IFT-81 with a poly-G linker (100 residues). The predicted structure looks very much like the crystal structure (PDB: 6IAN). I don't know if AF2 'looked at' the PDB structure, but 6IAN doesn't superimpose perfectly with AF2 structure

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@RuiZhangWUSTL

Rui Zhang

5 years

Can't wait to reconnect with my old #microtubule friends and make new #cilia #centrosome friends at the #ASCBEMBO2019 next week! I will present a poster (B173) on Sunday afternoon.

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@RuiZhangWUSTL

Rui Zhang

5 years

The EMDB and PDB entries for our recent #ciliary #doublet #microtubule paper are finally released, including two high-resolution structures of Rib72 and FAP166 mutants. Note we also included a 39-pages Data S1 (Protein Identification and Assessment Reports) in the Supplement.

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@RuiZhangWUSTL

Rui Zhang

4 years

Great work from Agard and Vale lab. The MIPs at the transition zone appear quite different from axoneme and basal body.

@JCellBiol

JCellBiol

4 years

Garrett A. Greenan, Ronald D. Vale, & David A. Agard @UCSF use electron cryo-tomography analysis of intact motile #cilia to elucidate how basal bodies template the formation of motile axonemes

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@RuiZhangWUSTL

Rui Zhang

2 years

Unbeatable salary and unbeatable papers, look no further👇

@DuLuLabs

DuLuLabs

2 years

Proud to be working at @VAInstitute , where we’re taking more steps to truly invest in our postdocs! The STARTING salary for postdocs will now be $70k, plus full benefits including retirement! Our labs are hiring, please contact us if you are interested.

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@RuiZhangWUSTL

Rui Zhang

3 years

Wonderful work from our future colleague 😁👇

@jenntrosome

Jenn Wang

3 years

Hi Twitter! I'm excited to present our preprint on centriole migration in olfactory sensory neurons. A super fun collaboration with the talented Katie Ching @StearnsLab

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Rui Zhang

2 years

Nice monitor!🤩 (and great science for sure)

@ChanZuckerberg

Chan Zuckerberg Initiative

@ChanZuckerberg

2 years

👋🏼 Meet imaging grantee Wah Chiu. His team @Stanford is #ImagingTheFuture to improve our understanding of how cells function in health + disease. With new #tech , researchers could watch the parasite that causes malaria invade healthy human cells to understand how to stop it

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@RuiZhangWUSTL

Rui Zhang

2 years

This approach appears to be very useful for the microtubule and dynein community 👇As usually, only the best work from Kai Zhang's lab 👏

@PengxinC

Pengxin Chai

2 years

The detailed "methods" we did to solve the structures of outer-arm dynein bound to microtubules (). I hope the approach can be applied to other Dyneins! Cannot wait to see more structures of motors bound to microtubules in different binding states!

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@RuiZhangWUSTL

Rui Zhang

5 years

My interactions with Michael is sporadic throughout the years, but I still remember the thrill when I talked to him for the first time in an elevator. It was also fun to have 1-on-1 breakfast with him at 7am when I visited Purdue. It is so sad to lose another giant of our time.

@PUCancerInst

Purdue Institute for Cancer Research

5 years

With great sadness we acknowledge the passing of @PUCancerCenter researcher Dr. Michael Rossmann. He was a brilliant researcher in the field of structural biology. We are ever grateful for his research and dedication. Read more about his legacy here:

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@RuiZhangWUSTL

Rui Zhang

4 years

👇Grant writing time, #cilia folks

@AlveoleLab

Alvéole

4 years

First Transmission Electron Microscopy images of SARS-CoV-2 virus isolated from patients attached to cilia of human respiratory epithelial cell. @Inserm 2/2 tweet: team membres @CNRS , Signia Therapeutics, CIQLE @UnivLyon1 , VirPath @ENSdeLyon , @Inserm #covid19 #coronavirus #cryoEM

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@RuiZhangWUSTL

Rui Zhang

5 years

This is the future of cryo-EM 👇

@zhou_lab

The Zhou Lab

5 years

Check out our new #cryoID approach to #structuralproteomics , from samples enriched directly from #endogenous sources, out today in @naturemethods . Brilliant work by @mimi1inh and @XRLI4 and everyone else involved! @TerwillTom @FitzpatrickLab

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Rui Zhang

2 years

This is so beautiful 😍 Congratulations to the team!

@OliBClarke

Oli B. Clarke

2 years

Happy to share our new @biorxivpreprint : “Architecture of the human erythrocyte ankyrin-1 complex” from Clarke lab (first author the incredibly talented @Frances84469032 ) and collaborators @lab_cali and @alexjamesnoble ! Read on to find out more (it was a surprise package…):

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@RuiZhangWUSTL

Rui Zhang

6 years

Faculty job application?

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Rui Zhang

4 years

Another γ-TuRC structure 👇

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Insights into the assembly and activation of the microtubule nucleator γ-TuRC

Nature - The cryo-EM structure of the γ-tubulin ring complex (γ-TuRC) from Xenopus laevis provides insights into the molecular organization of the complex, and shows that actin is a...

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@RuiZhangWUSTL

Rui Zhang

4 years

Absolutely beautiful cryoEM structure of centrioles!! 😍

@CentrioleLab

Paul & Virginie - Centriole Lab

4 years

It's out !! Our latest paper: A helical inner scaffold provides a structural basis for centriole cohesion. Big congratulations to Maeva, @nikolai_klena , @DavideGambarot2 and @MarineLaporte8 .

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Rui Zhang

5 years

One of my favorite scientists, a hero who opens a new field and allows many possibilities! Well deserved and long overdue

@uvahealthnews

UVA Health

5 years

Congratulations to researcher Edward H. Egelman, PhD, who has been elected to the National Academy of Sciences, one of the highest honors for a scientist @theNASciences @MedicineUVA

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@RuiZhangWUSTL

Rui Zhang

3 years

@BJ_Greber @CentrioleLab @Yoshi__Ichikawa @bengeliscious @StearnsLab @GaiaPigino @ReiterLab @PracheeAC @WallaceUcsf @LottePedersen12 @Mill_lab @Carter_Lab @David_S_Bristol @alanbrownhms @BastinLab_Paris Agree! Given no protein exists in isolation, I doubt if the prediction power of alphafold can go beyond a compact globular domain. For example, ciliary proteins such as FAP21 and FAP90 only become ordered upon binding to doublet microtubule platform 👇.

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@RuiZhangWUSTL

Rui Zhang

4 years

@LanderLab @Mengwooo Nice beard!

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@RuiZhangWUSTL

Rui Zhang

6 years

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Rui Zhang

2 years

🥳🥳🥳

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Rui Zhang

3 years

A lot can be learnt from this wonderful thread by the modeling expert 👇👏

@CrollTristan

Tristan Croll

3 years

There's (quite rightly) a lot of raving about the new AlphaFold DB resource today. Thought I'd weigh in with my own example. DNA-dependent protein kinase catalytic subunit () is an enormous beast - a single chain of 4,128 residues! (1/10)

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@RuiZhangWUSTL

Rui Zhang

1 year

Congratulations! @NogalesLab

@ShawPrize

The Shaw Prize

1 year

The #ShawPrize in #LifeScienceAndMedicine 2023 is awarded in equal shares to Patrick Cramer and Eva Nogales for pioneering structural biology that enabled visualization, at the level of individual atoms, of the protein machines responsible for #genetranscription . #ShawPrize2023

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@RuiZhangWUSTL

Rui Zhang

3 years

Come and join our legendary department 👇

@GarciaLabMS

Ben Garcia

3 years

Who wants to be my first hire? @WUSM_BMB is inviting applications for a tenure-track Assistant Professor working in any area of biochemistry or molecular biophysics. See for more details. URMs, women and diversity scientists are esp encouraged to apply!

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@RuiZhangWUSTL

Rui Zhang

4 years

A novel method that was not mentioned in previous literature

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@RuiZhangWUSTL

Rui Zhang

4 years

EB1 and actin filament inside primary cilia!

@cryoEM_Papers

cryoEM Papers

4 years

The molecular structure of primary cilia revealed by cryo-electron tomography

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@RuiZhangWUSTL

Rui Zhang

6 months

And another nice thing is that I can have hotpot everyday with my old and new friends😁

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@RuiZhangWUSTL

Rui Zhang

3 years

Very important finding! 👇

@Dev_Cell

Developmental Cell

3 years

New Short Article online now at Dev Cell: α-tubulin tail modifications regulate microtubule stability through selective effector recruitment, not changes in intrinsic polymer dynamics -

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@RuiZhangWUSTL

Rui Zhang

6 months

looks interesting! 👇 "A minority of final stacks yields superior amplitude in single-particle cryo-EM"

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A minority of final stacks yields superior amplitude in single-particle cryo-EM

Nature Communications - Here the authors develop an iterative particle sieving method called CryoSieve, demonstrating this method outperforms other cryo-EM particle sorting algorithms to reveal...

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@RuiZhangWUSTL

Rui Zhang

3 years

"What do you mean our manuscript is more suitable for a specialized journal?"

@AmymAisme

Amyish

3 years

Caption this

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@RuiZhangWUSTL

Rui Zhang

3 years

We also visualize a small molecule like density at the Taxol-binding site of β-tubulin.

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@RuiZhangWUSTL

Rui Zhang

4 months

I'll be there! 😁

@FASEBorg

FASEB

4 months

Join us in St. Paul! Since 2007, this conference has been the premier venue for cilia and flagella research. Organizers @PeterKJackson , @Mill_lab , and @ReiterLab have created a great program for our first SRC of 2024. #BCFSRC #CellBio Save your space:

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@RuiZhangWUSTL

Rui Zhang

3 months

Seems to be a very useful tool 👇

@BiophysicsGuy

Michael J. Robertson

3 months

Very excited to share our new preprint where we develop MIC, a deep metric learning tool for classifying waters & ions in experimental structures; fantastic collaboration with the extremely talented Laura Shub and Selina Liu from the Keiser lab!

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@RuiZhangWUSTL

Rui Zhang

6 years

My lab has a postdoc position available immediately. Please see the job post here. Please feel free to distribute.

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@RuiZhangWUSTL

Rui Zhang

2 years

This is a continuous collaboration with @alanbrownhms and @DutcherLab lab, following our previous work on the doublet microtubule and radial spoke from the best model organism Chlamydomonas. @miao_gui and @Xiangli_Wang66 are the co-first authors.

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