Basil Greber @BJ_Greber Twitter profile

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Basil Greber

@BJ_Greber

3 years

My twitter feed is a wild mix of imagery of a coup in the US and people excitedly discussing research papers.

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Basil Greber

@BJ_Greber

1 year

Behold the first pre-print from my lab at the @ICR_London ! Together with the groups of @SimakAli and @AbhayKot , we investigated inhibitor binding and selectivity for the CDK-activating kinase, an important target for future cancer drugs, using high-resolution 🍩 #cryoEM .

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Basil Greber

@BJ_Greber

4 months

Wait. Hold on. The guy who insisted that Sabatini absolutely had to be given a second chance (and offered $25 million in funding) after misconduct allegations and the guy who insisted that Claudine Gay absolutely had to leave after plagiarism allegations - is the same guy? 🤨🤨🤨

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Basil Greber

@BJ_Greber

4 years

Some good news at the end of the year: After almost 5 years in the @NogalesLab at UC Berkeley, I will move to London in mid-2020 to start my own lab at the @ICR_London . A heartfelt "thank you" to all those who supported me along the way.

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Basil Greber

@BJ_Greber

4 years

It's time to properly announce that I have started my own lab at the @ICR_London ! It's certainly a strange time to start a lab, but I have had wonderful support both from the ICR and from @NogalesLab to facilitate the transition. Thank you! New website:

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Basil Greber

@BJ_Greber

1 year

You have no idea how long I waited for this moment. 😂 Coming soon! #cryoEM

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Basil Greber

@BJ_Greber

2 months

Our paper on high-resolution 🍩 #cryoEM of the human CDK-activating kinase for structure-based drug design has been published in @NatureComms ! We determined more than a dozen 2 Å structures of the ligand-bound complex to investigate the mechanism of inhibitor selectivity.

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Basil Greber

@BJ_Greber

4 years

When it comes to the ongoing political crisis in the US, @sarahkendzior ’s warnings were among the earliest & darkest, but also among the most accurate. The scholar for authoritarian regimes was interviewed by @AnandWrites .

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Basil Greber

@BJ_Greber

1 month

My take on high-resolution #cryoEM of small complexes has just been published in @Structure_CP . In my review, I first introduce how technical developments have enabled #cryoEM structure determination of ever smaller targets at better and better resolutions.

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Basil Greber

@BJ_Greber

3 months

Our first "lab paper" is out: In collaboration with Sandro Ataide/Yichen Zhong (University of Sydney) and @AdrianKoh8 / @AbhayKot (TFS), @JunjieFeng5 determined the structure of the peptide binding domain of SRP68/72, the largest protein components of the eukaryotic SRP.

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Basil Greber

@BJ_Greber

2 years

Our structure of the cyanobacterial phycobilisome is finally out! Amazing stuff by @TinaDomnguez , @paulvictorsauer and colleagues ( @NogalesLab , @HenningKirst , KerfeldLab). High resolution #cryoEM , multiple conformations, inhibited state, it's all in there.

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Basil Greber

@BJ_Greber

3 years

New #cryoEM textbook! Edited by @NogalesLab , @chiulab and the wonderful Bob Glaeser. Contributions by many experts in the field (too many to list them here).

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Basil Greber

@BJ_Greber

3 years

Some good news before the end of the year: An MRC/UKRI Career Development Award will fund my lab @ICR_London for 5 years. Looking forward to more #cryoEM of molecular assemblies involved in DNA repair and exciting collaborations! Thanks to all those who made this possible!

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Basil Greber

@BJ_Greber

3 years

New paper: 2.5 Å-resolution structure of the 85 kDa-CDK/cyclin module of the human CAK in complex with ICEC0942, an inhibitor currently undergoing clinical trials for cancer treatment. Data: @NogalesLab , written: @ICR_London , collaboration with @simakali1 .

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Basil Greber

@BJ_Greber

5 years

Our TFIIH paper, previously posted on @biorxivpreprint , has now been published in @eLife : The complete structure of the human TFIIH core complex. Interested in #transcription , #DNArepair , #helicases , or #VPP (phase-plate) #cryoEM ? Have a look!

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Basil Greber

@BJ_Greber

3 years

New lab, who dis? 🙃 Time to order more stuff.

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Basil Greber

@BJ_Greber

4 months

El Pais article on Eva @NogalesLab : “The daughter of a shepherd and an embroiderer is in the running to win a Nobel Prize” via @elpaisinenglish

The daughter of a shepherd and an embroiderer is in the running to win a Nobel Prize

The Spanish biophysicist Eva Nogales has just received the $1.2 million Shaw Prize for revealing the atomic intricacies of human beings

english.elpais.com

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Basil Greber

@BJ_Greber

4 years

Our manuscript on the structure of the human CDK-activating kinase has just been made available on @biorxivpreprint . Thanks to @Juanmabertoldi1 , Kif Lim, Tony Iavarone (mass spec), Dan Toso (Berkeley #cryoEM ) and Eva @NogalesLab for their support.

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Basil Greber

@BJ_Greber

5 years

My first @biorxivpreprint is out: The complete structure of the human TFIIH core complex. #NogalesLab #cryoEM #transcription #DNArepair

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Basil Greber

@BJ_Greber

3 years

Open post-doc position in my group at @ICR_London for mechanistic studies of DNA repair, using #cryoEM (of course) and biophysical methods. Long-term aim is to understand structures & mechanisms well enough to target repair pathways using small molecules.

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Basil Greber

@BJ_Greber

1 year

Structure of a pre-60S subunit stuck in the nuclear pore. This is the kind of thing we used to joke about 10 years ago, and we thought it was very (!) funny because ridiculous and completely impossible. Now someone actually did it! Amazing stuff. #cryoEM

Nuclear export of pre-60S particles through the nuclear pore complex

Nature - We report the cryo-electron microscopy structure of native pre-60S particles trapped in the channel of the yeast nuclear pore complex, suggesting a translocation model for the export of...

www.nature.com

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Basil Greber

@BJ_Greber

3 years

Already one year in London. It has not been the smoothest ride (for obvious reasons), but we are about to get off the ground - with a new lab space suitable for my soon-to-be group of four, funding for 5 years, and lots of work to do. Thanks to everyone for their support.

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Basil Greber

@BJ_Greber

2 years

Too hard to resist the #ModelAngelo frenzy. Gave it a go using our small CDK-activating friend. Map computed from 4 hours worth of Glacios 2 data, live processing, 2 hours worth of RELION processing, and then automated modelling without sequence. The quality is quite impressive.

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Basil Greber

@BJ_Greber

5 years

Refreshing the mind in the High Sierras. @NikonUSA #D810 #20mm f/1.8 G. #Yosemite & #MonoLake .

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Basil Greber

@BJ_Greber

3 years

My lab at the @ICR_London is recruiting a postdoc interested in uncovering the mechanisms of DNA repair! Candidates with expertise in the biochemistry of nucleic acid/protein complexes or in #cryoEM are particularly encouraged to apply. Please have a look!

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Basil Greber

@BJ_Greber

3 years

New #CAFR : The @ICR_London . Testing our new #Glacios with a Falcon 4 DED. #cryoEM 🍩

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Basil Greber

@BJ_Greber

3 years

OMG. 🤯

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Basil Greber

@BJ_Greber

4 years

Goodbye California!

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Basil Greber

@BJ_Greber

4 years

What remains after 5 years of Berkeley postdoc: The great grid box graveyard. #cryoEM #cleanup

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Basil Greber

@BJ_Greber

2 years

I guess that's me! If you like #cryoEM of small protein complexes, exploration of interactions between small molecules and protein targets, or 🍩-densities, please join us on the 18th!

Microscopy & Spectroscopy

@thermosciEMSpec

2 years

We're continuing our Glacios 2 Cryo-TEM excitement with a special webinar on Oct. 18 featuring Dr. Basil Greber ( @BJ_Greber ) of @ICR_London . Register to hear how his team used the very first Glacios 2 to facilitate next-gen #cryoEM #DrugDiscovery :

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Basil Greber

@BJ_Greber

1 year

Open postdoctoral position in my lab at the @ICR_London to study DNA repair using #cryoEM ! If you are interested in structural biology and/or biochemistry and molecular biology of DNA repair processes, please have a look (and apply)!

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Basil Greber

@BJ_Greber

6 years

The latest ribosome structure from @The_Ban_Lab : The Trypanosome mitochondrial ribosome, by David Ramrath et al. Never seen anything like it: 400 Angstrom diameter, 127 proteins, the largest almost 2000 aa long. Mind-blowing. Congratulations!

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Basil Greber

@BJ_Greber

2 years

Having manually checked 1300 water molecules (and manually built a few 100s), I can still see COOT's blue density mesh when I close my eyes. Yes, that's a good thing. #cryoEM

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Basil Greber

@BJ_Greber

3 years

Another epic achievement, another 2+ papers squeezed into one: Structures of human mediator and the full human PIC from the Xu lab.

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Basil Greber

@BJ_Greber

3 years

First landscape photography trip in the UK. Cornwall is very nice. 🙃 #Nikon #landscapephotography #seascape

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Basil Greber

@BJ_Greber

4 years

Right on time for my arrival at the @ICR_London , there's a @NogalesLab paper out, with my brand new e-mail address on it: Our paper on the structure of the CDK-activating kinase has been published online in @PNASNews . Thanks again to everyone involved!

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Basil Greber

@BJ_Greber

4 years

Some departmental love at my last @berkeleyMCB retreat. Thank you to all those who supported me during my stay in Berkeley - and of course special thanks to Eva @NogalesLab !

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Basil Greber

@BJ_Greber

3 months

The use of BLUSH for our recent structure has (unsurprisingly) generated considerable interest. I have appended the "how it started" vs. "how it's going" below.

Basil Greber

@BJ_Greber

3 months

Our first "lab paper" is out: In collaboration with Sandro Ataide/Yichen Zhong (University of Sydney) and @AdrianKoh8 / @AbhayKot (TFS), @JunjieFeng5 determined the structure of the peptide binding domain of SRP68/72, the largest protein components of the eukaryotic SRP.

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Basil Greber

@BJ_Greber

2 years

New work from our @ICR_London neighbours: The lab of @calfieri84 presents the #cryoEM structure of a nucleosome-bound MuvB transcription factor complex, including an approx. 70 kDa module resolved at 3.5 Å using Glacios/F4 data. Congratulations!

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Basil Greber

@BJ_Greber

3 years

Big box with new toys! Getting ready for October, when a PhD student will join my lab. 😉

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Basil Greber

@BJ_Greber

3 years

As we go into lockdown once more, looking back on the last 10 years of travel and photography seems to be the best way to appreciate the beauty of our planet and the great outdoors. Stay safe everyone.

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Basil Greber

@BJ_Greber

6 years

Now out in @sciencemagazine : Structure of human TFIID and mechanism of TBP loading onto promoter DNA, by Avi Patel et al. #cryoEM #NogalesLab

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Basil Greber

@BJ_Greber

2 years

Former graduate student extraordinaire (now postdoc) @avibpatel_ with his first last-author paper. Congrats to everyone involved - @StefanZukin , @NogalesLab . #cryoEM

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Basil Greber

@BJ_Greber

4 years

Sunset over the Bay Area. Cycling/walking up the hill is a good isolation workout, and watching the sun go down over the bay brings some peace to these troubled times. #California #SanFrancisco #Berkeley #NikonNoFilter #D810 #Cityscape

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Basil Greber

@BJ_Greber

3 years

Welp. I guess #cryoEM of small complexes was fun while it lasted. #AlphaFold

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Basil Greber

@BJ_Greber

2 years

@strnr @sheilateves Glad you also told us where not to download the tool. Appreciated. 😇

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Basil Greber

@BJ_Greber

4 years

After 4 years and 11 months of post-doc in the @NogalesLab , I finally got a desk with a view. Not for long. #BackAtWork

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Basil Greber

@BJ_Greber

4 years

View of #Oakland , #Berkeley , #SanFrancisco after sunset. Taken with @NikonUSA #D810 .

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Basil Greber

@BJ_Greber

7 months

This was missed due to major live events happening around the same time, but we have released some of our high-resolution (2 Å) and rapid screening #cryoEM maps & models of the human CAK bound to small molecules. Paper still under review - more to follow.

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Basil Greber

@BJ_Greber

4 years

#Cityscape photography in #London . @NikonUSA Z7 with 20/1.8. That cloudless skies are something I'd have to worry about in the UK is... unexpected.

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Basil Greber

@BJ_Greber

2 years

Structure validation and peer review still an issue in the #cryoEM field. I now routinely ask for maps and models when reviewing and the number of cases that raise concerns is substantial. I often suspect that I was the only reviewer that looked at them.

David M Thal

@Thal_DM

2 years

This is a rather egregious case, and there are many errors in the modelling around the receptor and ligand(s). These errors affect the premise of the paper. How was this not picked up in peer review @NatureComms or prior to. Did any reviewers look at the EM maps or model?

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Basil Greber

@BJ_Greber

1 year

The technically-inclined might wonder how the equipment used affected our ability obtain those 🍩 maps with well-resolved aromatics and ordered waters. We performed two comparisons, using the exact same grid for each pair of data in each experiment.

Basil Greber

@BJ_Greber

1 year

Behold the first pre-print from my lab at the @ICR_London ! Together with the groups of @SimakAli and @AbhayKot , we investigated inhibitor binding and selectivity for the CDK-activating kinase, an important target for future cancer drugs, using high-resolution 🍩 #cryoEM .

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Basil Greber

@BJ_Greber

1 year

Because I encountered an example of a direct interaction in a paper where alphaFold multimer failed to predict any interaction, I was wondering if anyone was doing proteome-wide alphaFold protein-protein interaction screens. Found this - should try:

AlphaPulldown – a Python package for protein-protein interaction screens using AlphaFold-Multimer

Summary The Artificial Intelligence-based structure prediction program AlphaFold-Multimer enabled structural modelling of protein complexes with unprecedented accuracy. Increasingly, AlphaFold-Mult...

www.biorxiv.org

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Basil Greber

@BJ_Greber

2 years

Behold the amazing phycobilisome, brought to you by @paulvictorsauer ( @NogalesLab ), @TinaDomnguez (Kerfeld Lab) and colleagues! We have two new pre-prints, one about the PBS structure () and one about its regulation by OCP (). #CryoEM

Structure of the Quenched Cyanobacterial OCP-Phycobilisome Complex

Photoprotection is an essential mechanism in photosynthetic organisms to balance the harvesting of light energy against the risks of photodamage. In cyanobacteria, photoprotective non-photochemical...

www.biorxiv.org

Paul Sauer

@paulvictorsauer

2 years

Our complete 2.7A structure of a cyanobacterial phycobilisome reveals unexpected conformational changes, the position of the quencher and even a completely new protein! Great collaboration with @TinaDomnguez at the Kerfeld lab. Preprints below! #CryoEM @UCBerkeley @LBNLBioSci

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Basil Greber

@BJ_Greber

4 years

Exciting times at @ICR_London ! Our in-house instrument has been delivered and is being built as we tweet! You may not see it but we have big smiles under our masks. 😬

Sebastian Guettler Lab

@SGuettlerLab

4 years

Looks like a new era of Structural Biology and #cryoEM has begun @ICR_London : our new Glacios TEM has arrived in its brand-new home, here presented by new Team Leaders @calfieri84 and @BJ_Greber . The future is bright! Thank you to all who made it happen!

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Basil Greber

@BJ_Greber

3 months

Paper accepted. Yay!

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Basil Greber

@BJ_Greber

2 years

Structural analysis of mutant, GTP-bound tubulin by @bjlafrance and colleagues from the @NogalesLab & @surrey_lab provides new insight into microtubule structure and dynamics. #cryoEM

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Basil Greber

@BJ_Greber

3 years

Nice! Our CAK-inhibitor #cryoEM paper with @NogalesLab and @simakali1 not only graced the cover of @BiophysJ but was also named the editor's pick and featured on the journal's blog:

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Basil Greber

@BJ_Greber

3 years

Weekend fun with CryoSPARC live. CAK (85 kDa) with a bound compound. If we add the other half of the data and move it into RELION, how far will it go? 🙃 Project with @AbhayKot @AdrianKoh8 @cushing_v @JunjieFeng5 @simakali1 @FuchterMatt . #cryoEM #liftoff

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Basil Greber

@BJ_Greber

3 years

New structural biology conundrum: Do I benefit more from I using my GPU time for improving my #cryoEM map using RELION or by predicting the structure of my target using ALPHAFOLD?

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Basil Greber

@BJ_Greber

4 years

Laboratory woes: Protein: Good job on that purification! I'm doing just fine! Look, I'm swimming in completely clear solution! Me: Ok, let's load you onto gel filtration. With this nice syringe. I'll be gentle. Protein: Syringe? Now I am white as milk! LOL! You fool! Me: 😳😭

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Basil Greber

@BJ_Greber

3 years

Hallo @tagesanzeiger - Sie haben vor kurzer Zeit einen langen Artikel veröffentlicht, in dem basierend auf der Arbeit Klaus Stöhrs behauptet wurde, die britische COVID-Variante hätte keinen Einfluss auf COVID-Fallzahlen. Haben Sie die zu Grunde liegenden Zahlen überprüft?

Cornelius Roemer

@CorneliusRoemer

3 years

Stöhr wird immer dreister mit seinen Lügen und Desinformationen. Das ist wissenschaftliches Fehlverhalten. Heute behauptet er, der #B117 -Anteil in UK sei am 15. Januar bei 50% gelegen. Das ist sehr falsch. Bereits 1 Monat früher, ca. 15. Dezember war Anteil 50% (s. 3. Grafik) 1/

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Basil Greber

@BJ_Greber

3 years

Crossing this bridge every day on my way to work, this picture started forming in my mind. Yesterday, I finally got around to bringing a camera & tripod to capture it. #London #CityScape captured with @UKNikon #NikonZ7 .

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Basil Greber

@BJ_Greber

7 years

The cryo-EM structure of human TFIIH. Thanks to my awesome co-authors for their contributions to this project.

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Basil Greber

@BJ_Greber

5 years

@pipcosper Good to know that „unfortunate delays“ have been around for a while...

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Basil Greber

@BJ_Greber

5 years

Our latest paper on #cryoEM of bacterial microcompartments is out @Structure_CP . Fun collaboration with Markus Sutter and the Kerfeld Lab @LBNLBioSci / @michiganstateu .

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Basil Greber

@BJ_Greber

3 years

Application deadline for this postdoc position in my lab is next Sunday (March 21st). If you are interested in DNA repair and have expertise in structural biology and/or biochemistry of nucleic acid-protein complexes, please have a look at the ad⬇️ and our website - and apply. 😉

Basil Greber

@BJ_Greber

3 years

Open post-doc position in my group at @ICR_London for mechanistic studies of DNA repair, using #cryoEM (of course) and biophysical methods. Long-term aim is to understand structures & mechanisms well enough to target repair pathways using small molecules.

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@BJ_Greber

3 years

Casually dropping the sub-3 Å #cryoEM reconstruction of a 110 kDa-protein in the supplement: "Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from tilted grids." Well done @avibpatel_ & team! @NogalesLab

Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from...

Recent technical developments have made single particle cryo-EM a major structural biology technique, especially in the characterization of challenging samples that resist crystallization, can only...

www.biorxiv.org

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Basil Greber

@BJ_Greber

1 month

Thrilled to hear that @Structure_CP has designated my review on small-complex #cryoEM as a featured article in their April issue. If you have not done so yet, have a look here:

Basil Greber

@BJ_Greber

1 month

My take on high-resolution #cryoEM of small complexes has just been published in @Structure_CP . In my review, I first introduce how technical developments have enabled #cryoEM structure determination of ever smaller targets at better and better resolutions.

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Basil Greber

@BJ_Greber

4 months

Exactly 10 years ago: Our first direct detector adventures @The_Ban_Lab . I do not think we had any idea how wild the next decade in #cryoEM was going to be.

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Basil Greber

@BJ_Greber

7 years

RNA Polymerase + Ribosome = Expressome. Awesome, Rebecca! #cryoEM #ribosome

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Basil Greber

@BJ_Greber

3 years

Another take on the #TowerBridge #London .

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Basil Greber

@BJ_Greber

6 years

Kelly Nguyen's latest breakthrough structure - human telomerase holoenzyme with bound substrate! #cryoEM

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Basil Greber

@BJ_Greber

3 months

It may be worth noting that "standard" RELION 4 gave 2.9 Å resolution, cryoSPARC 3DFlex gave 2.7 Å (with a larger particle set) and RELION 5/BLUSH gave 3.0 Å. But the map quality of the latter was clearly the best, and using that map was the obvious choice.

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Basil Greber

@BJ_Greber

4 years

I have a Talos Arctica joke, but it's kind of low-energy. #cryoEM

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Basil Greber

@BJ_Greber

2 years

FYI - we now have a lab Twitter feed and a pretty picture to show you. #cryoEM

GreberLab

@greberlab

2 years

First lab meeting today, and among the things we discussed was @cushing_v 's recent 1.9 Å-resolution reconstruction of an inhibitor-bound CAK complex (true 85 kDa - part of MAT1 is deleted). These stunning data were collected by @AdrianKoh8 and @AbhayKot . Thank you!

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Basil Greber

@BJ_Greber

2 years

First attempts at image stacking for night photography: Milky Way, shot at Crater Lake, Oregon. @NikonUSA Z7 and 14-24/2.8 @ 14/2.8, 7 frames stacked in Photoshop. Amazed by the performance of the lens and the camera.

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Basil Greber

@BJ_Greber

4 years

@hjf_mueller Schweizer hier. Habe in meinem Leben nur einmal persönlich gewählt, als ich vergessen hatte, den Briefumschlag rechtzeitig zur Post zu bringen. Bin weder alt noch krank. Wo ist das Problem?

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Basil Greber

@BJ_Greber

5 months

I don't have a structure to drop but I re-submitted a manuscript. Please clap. 😆

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Basil Greber

@BJ_Greber

1 year

About half of the papers I review do not show the model-map FSC. If you want to know why I will ALWAYS ask for it, no matter how nice your resolution, read the thread below. #cryoEM

TJ McCorvie (@allostericstate on ☁️)

@allostericstate

1 year

#cryoEM #structuralbiology 🔬🧊 A thread🧵...Due to the egregious structural data discussed a few weeks ago I feel there should be a discussion about how we could possibly improve the process in which EM maps and models are validated before deposition. /1

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Basil Greber

@BJ_Greber

10 months

My journey in the empire of the midnight sun has come to an end. Thank you Norway 🇳🇴, this was amazing.

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Basil Greber

@BJ_Greber

1 year

"Postdoctoral students." Give me a break.

The New York Times

@nytimes

1 year

Postdoctoral students and academic researchers at the University of California said they will return to work on Monday after ratifying a new contract. But workers who help teach undergraduates and assign grades remain on strike.

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Basil Greber

@BJ_Greber

3 years

Taking a little break in Cornwall, socially distanced on slippery rocks at the edge of the sea. #landscapephotography #seascape #nikonphotography

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Basil Greber

@BJ_Greber

2 years

@LugerLab @nsmb The people at North Shore Mountain Biking magazine ( @nsmb ) may be disappointed to learn that all that money is not coming their way after all. 😉

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Basil Greber

@BJ_Greber

4 years

Amazing work using @DTegunov 's software M: Ribosome at better than 4 Angstroms resolution from in-situ data reveals bound antibiotic. 🤯

cryoEM Papers

@cryoEM_Papers

4 years

Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.7 A inside cells

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Basil Greber

@BJ_Greber

7 years

Wow! Structure of a 17 MDa complex at 3.5 Angstroms resolution! So much information...

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Basil Greber

@BJ_Greber

1 year

On-grid purification of protein complexes using affinity grids and a 3D printed flow cell. Cool stuff by our London neighbours under Chris Aylett's leadership:

On-grid purification of electron microscopy samples via a 3D-printed flow-cell

While recent advances in cryogenic electron microscopy coupled with single particle analysis have the potential to allow structure determination in a near-native state from vanishingly few individual...

www.biorxiv.org

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Basil Greber

@BJ_Greber

2 years

Sunset afterglow illuminates the sky behind Big Ben. #London

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Basil Greber

@BJ_Greber

1 year

The link to the pre-print is here. Thanks to everyone involved in the project - @cushing_v and @JunjieFeng5 from my lab, @AdrianKoh8 and @AbhayKot from TFS, Kaste Jurgaityte and @simakali1 from Imperial College, and Ash Bahl from Carrick Therapeutics.

High-resolution cryo-electron microscopy of the human CDK-activating kinase for structure-based...

Rational design of next-generation therapeutics can be facilitated by high-resolution structures of drug targets bound to small-molecule inhibitors. However, application of structure-based methods to...

www.biorxiv.org

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Basil Greber

@BJ_Greber

1 year

And we conclude the thread with the meme-summary that many of you seemed to enjoy quite a bit. 😉

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Basil Greber

@BJ_Greber

4 years

I wish I also had a Titan Krios joke, but I can't afford one.

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Basil Greber

@BJ_Greber

3 years

What an unnecessarily stupid headline with entirely unnecessary & misleading implications.

The New York Times

@nytimes

3 years

China has approved its fifth Covid-19 vaccine, and it’s made from the ovary cells of hamsters.

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Basil Greber

@BJ_Greber

5 months

Goodbye 2023. 👋

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Basil Greber

@BJ_Greber

2 years

My photographic 2021. London and the Cornwall coast replaced the Californian wilderness and the Nordic outdoors. For now. Shot using @UKNikon Z7 & a few nice lenses. 😉

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Basil Greber

@BJ_Greber

6 months

Some of us keep telling people that #cryoEM is easy and everyone can do it and it does not require much skill, and this is the logical result. Who reviews these papers? 😵‍💫

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Basil Greber

@BJ_Greber

3 years

Ironic that they used for illustration a complex that alpha fold has no chance of predicting correctly.

nature

@Nature

3 years

A 'transformative' AI tool called AlphaFold has predicted the structure of nearly the entire human proteome — and there's more to come.

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Basil Greber

@BJ_Greber

1 year

So I bought a 400 mm telephoto and thought I could do some astrophotography with it. Having tried once, I now want an 800 mm telephoto lens. Or a telescope (the below is a 6 MP crop from a 45 MP frame). Why did nobody warn me about the financial risks of getting into astro? 😆

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Basil Greber

@BJ_Greber

4 years

@hjf_mueller Wer glaubt, dass es ein Problem ist, wenn eine grössere Anzahl der Stimmberechtigten von ihrem Stimmrecht gebrauch macht, ist selbst Teil des wirklichen Problems: Voter Suppression.

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Basil Greber

@BJ_Greber

4 years

@NoahShachtman Seems relevant: "evening curfews to try to stop young people from gathering socially, [...] contributing to the spread [...] most of the contagion that happens within multigenerational homes comes from young people bringing it in." Someone has to bring it into those homes!

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Basil Greber

@BJ_Greber

5 years

The only sane way to deal with the current madness: Camp out atop a mountain, disconnected from the world (and the cell phone signal). But proteins have to be expressed and grant applications have to be written, so I duly returned to work.

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