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Séamus Holden Profile
Séamus Holden

@seamus_holden

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Associate Professor, University of Warwick. Bacterial cell biology & biophysics. Super-resolution microscopy. @seamusholden .bsky.social

Warwickshire, England
Joined April 2015
Don't wanna be here? Send us removal request.
@seamus_holden
Séamus Holden
5 years
Scientific highlight of last year: Last month I used our lab's new 3D printer to create a replacement wheel for my 4 year old's broken Transformer. When I presented it to him, he solemnly told me "Daddy, you are a great scientist". Nobel winners, eat your heart out.
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@seamus_holden
Séamus Holden
3 years
Me, the PI, returning to the wet lab this week after a very long absence
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@seamus_holden
Séamus Holden
3 years
Exciting news about a new adventure: I and most of the Holden lab are moving to @warwickuni @WarwickLifeSci new Interdisciplinary Biosciences Research Building in spring next year! And a promotion to Associate Professor (Reader). PhD and PDRA position available - details soon
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@seamus_holden
Séamus Holden
11 months
We just got a @wellcometrust Discovery Award to work with @SaricLab and Jan Löwe's labs on the mechanistic principles of bacterial cell division! So excited to work with these amazing people on this important and fascinating problem!
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@seamus_holden
Séamus Holden
2 years
Delighted to announce @HenrikStrahl , Waldemar Vollmer, and myself have been awarded a @BBSRC grant to investigate mechanistic principles of bacterial cell wall construction by the elongasome!
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@seamus_holden
Séamus Holden
6 years
Our second child arrived this morning, he and his mummy are doing great! And the NHS staff at Newcastle Royal Victoria Infirmary were incredible. Signing off Twitter for a little bit, see you all soon.
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@seamus_holden
Séamus Holden
3 years
So happy our paper using badass microscopy to figure out the function(s) of FtsZ treadmilling in Bacillus subtilis cell division is out! Immensely proud of the work of all authors on this study over the last 4 years, in face of obvious global challenges.
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@seamus_holden
Séamus Holden
7 years
Just got awarded a Sir Henry Dale @wellcometrust fellowship! Aww yeah 😀
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@seamus_holden
Séamus Holden
3 years
My little boys (possibly+mum) made me an awesome bacillus birthday cake! 6yo made the marzipan rods with division septa. Microscale bacilli sprinkles courtesy of 2yo.
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@seamus_holden
Séamus Holden
9 months
I have a 3 year post doc position available in my lab at @WarwickLifeSci . Focus is on bioimage analysis of bacterial single molecule tracking and super-resolution microscopy data. Application deadline is 10th September.
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@seamus_holden
Séamus Holden
4 months
🚨JOBS🚨 Two 4 year post-docs available in my lab at Warwick. Looking for experts in super-resolution microscopy, single molecule fluorescence, FRET or protein biochemistry. Visa+NHS fees covered. Salary~£40K. Two exciting team science projects with outstanding collaborators
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@seamus_holden
Séamus Holden
4 years
Really excited to share this with the world. We used ultra-sensitive custom microscopy to figure out why cytoskeletal filament motility is so important for bacterial cell division. Aka - why are bacterial protein sticks wriggly?
@biorxiv_micrbio
bioRxiv Microbiology
4 years
FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in bacterial cell division #biorxiv_micrbio
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@seamus_holden
Séamus Holden
7 years
Post-doc position in my lab: Push the limits of super-resolution and figure out bacterial cell division
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@seamus_holden
Séamus Holden
7 years
We found that a motile cytoskeleton drives bacterial cell division @cees_dekker @BrunLabIU @mvannieu @fleshball
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@seamus_holden
Séamus Holden
5 years
Dear Twitter/ scitwitter, at 2k followers, I paused to reflect on this whole scientific social media thing. I must say, I have got more out of this than I ever expected. I started a Twitter account when I set up my lab, and I thought it would just be a way to find some papers 1/4
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@seamus_holden
Séamus Holden
2 months
In work led by @KevinDWhitley , we demonstrate that septal cell wall synthesis in Bacillus subtilis follows a one-track model, where both the motion and activity of the septal PG synthesis machinery is asynchronous with FtsZ dynamics
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@seamus_holden
Séamus Holden
4 years
Huge thanks @BritBiophysSoc for this year's BBS Early Career Award! And sincere deep gratitude to all Holden lab members past and present for your passion and hard work.
Importance announcement: BBS prizes! All three prize winners will be presenting at the biennial meeting. Our sincere congratulations to John Christodoulou, Seamus Holden @seamus_holden and Daniel Hurdiss. Details are below.
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@seamus_holden
Séamus Holden
5 years
You know what gets my goat? The relentless drive towards certainty in science, especially paper discussion sections. What's wrong with good old doubt, and frankly discussing uncertainties in your data and your model?
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@seamus_holden
Séamus Holden
6 years
So much scientific software dies when the authors abandon it. Believe me I understand the constraints that cause authors to stop actively developing/ supporting software - it all comes down to lack of funding mechanism. But boy does it drive me crazy
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@seamus_holden
Séamus Holden
4 years
Dressed up for @lmr_1994 's Zoom viva today. Doctoral examinations are almost the only time I wear a suit professionally, out of respect for the many years of work of the candidate. I see no reason to change this for some dumb virus!
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@seamus_holden
Séamus Holden
6 years
A list of lectures and information about microscopy - with the fantastic iBiology series very much top of the bill:
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@seamus_holden
Séamus Holden
4 years
This is what 130,000 gloves look like! +95 safety glasses, 3 face shields, 170 disposable surgical face masks, 8 coveralls . To be collected Monday by Newcastle Gateshead CCG for frontline GPs, dentists, hospitals. Thank you @UniofNewcastle scientists!! #getPPE_NCL
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@seamus_holden
Séamus Holden
5 months
I still have 2 4-year post doc jobs available, will advertise early January. One is for a super resolution microscopist to image bacterial cell division, other is for biochemist or single molecule biophysicist to use single molecule FRET to study bacterial cell wall synthesis
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@seamus_holden
Séamus Holden
3 years
🚨😀Cool job alert😀🚨Excited to announce a _permanent_ quantitative microscopy staff scientist position at the Centre for Bacterial Cell Biology to collaborate on advanced microscopy and quantitative image processing with world class bacteriology labs 1/7
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@seamus_holden
Séamus Holden
5 years
It begins! Build of the new Holden lab STORM scope. Fully open source blueprints to follow
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@seamus_holden
Séamus Holden
1 year
We are recruiting 4 Assistant/ Associate Professor positions in microbiology at @WarwickLifeSci ! 2 on Host-virus interactions, 1 on Plant virology, 1 on Host responses to microbes. . Please RT!
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@seamus_holden
Séamus Holden
8 months
This is a collection of resources for introducing new students and staff to microscopy:
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@seamus_holden
Séamus Holden
3 years
Save the date: Physics of Life conference organized jointly by several British biophysics groups and societies. Mar 27-30 2023
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@seamus_holden
Séamus Holden
4 years
In work led by Ling Wu and @Errington_Lab ,we investigated how physical confinement affects cell wall free bacteria. My favourite finding:all you need for robust chromosome segregation is physical confinement in a narrow channel (left vs right panel),
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@seamus_holden
Séamus Holden
6 years
I am so excited about the 2D galvo that arrived today for ring-TIRF/ HILO on our microscope! Look, it can spin laser beams around!
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@seamus_holden
Séamus Holden
11 months
When you get the bubbly out and the labs next door are all biochemists 🤣
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@seamus_holden
Séamus Holden
6 months
I am readvertising this exciting super-resolution/ single molecule post-doc position, with two major changes. Start date flexible. Prior bacteriology expertise not required. 1) Post is now for 4 years - rationale below 1/2
@seamus_holden
Séamus Holden
8 months
We are recruiting a post-doctoral researcher in experimental biophysics and super-resolution microscopy. Post is for 5 years, extendible up to a total of 8 years. Details here: . Application deadline: 10 October 2023
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@seamus_holden
Séamus Holden
2 years
Good news: we got a @UKRI_News 21ALERT grant for a @zeiss_micro Lattice SIM2 with FRAP & photoactivation at @WarwickLifeSci ! This will allow us to establish live cell SIM at Warwick in a range of organisms from bacteria all the way to plants 1/2
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@seamus_holden
Séamus Holden
8 months
We are recruiting a post-doctoral researcher in experimental biophysics and super-resolution microscopy. Post is for 5 years, extendible up to a total of 8 years. Details here: . Application deadline: 10 October 2023
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@seamus_holden
Séamus Holden
4 years
First day back in the lab. Theia, our custom microscope, is happy to see me!
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@seamus_holden
Séamus Holden
10 months
We found that B subtilis divisome synthesis complexes are not coupled to treadmilling FtsZ filaments in either their active or inactive states. Rather, divisome processive motion around septum is exclusively driven by cell wall synthesis
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@seamus_holden
Séamus Holden
5 years
We've just completed the latest updates to the 3D super resolution challenge. Lots of cool new additions, especially analysis on real 3D data, and web tools for interactive data exploration
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@seamus_holden
Séamus Holden
2 years
How I feel about #UCUstrikes and @UCU right now: Angry and demoralized, and that a lot of mine and everyone else's time, money and effort in strike action has been compromised through poor leadship, poor strategy and a fairly disfunctional union. 1/4
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@seamus_holden
Séamus Holden
6 years
Post-doc job: looking for an awesome bacterial cell biologist/ cell division/ cytoskeleton person to join my lab to unravel organization of bacterial division machinery with super-res microscopy. Super-res expertise not required - we will teach you!
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@seamus_holden
Séamus Holden
4 years
If you are interested in Bacillus subtilis and related organisms, join us for Subtillery, June 8-12, a virtual conference organized by @Eswara_Lab . We have amazing speakers, almost all PDRAs and PhDs, from outstanding labs all over the world!
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@seamus_holden
Séamus Holden
2 years
Moving day! Sad to leave all our Newcastle friends but excited to move into a gorgeous new home and not have to do 2000 miles of driving and AirBnB again next month😀
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@seamus_holden
Séamus Holden
5 years
Finished building the new microscope body in time for the weekend 😀 Full cad models soon
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@seamus_holden
Séamus Holden
3 years
In an entirely remote research project, @NewcastleMedSch undergrad members of our lab managed to do bacteriology in their bedrooms with a £30 microscope objective and a DIY @OpenFlexure microscope. How cool is that?!
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@seamus_holden
Séamus Holden
4 years
When your 5yo makes you a 34 today badge, you wear it!
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@seamus_holden
Séamus Holden
2 years
God the trend on here of overly confident advice threads is doing my head in.
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@seamus_holden
Séamus Holden
4 years
My sister is a frontline NHS doctor and I had to post her my lab's face shield and eyewear because her hospital has nothing to issue her with. And these psychopaths talk about people's feelings being hurt. Doctors and nurses are literally dying from their logistics failure.
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@seamus_holden
Séamus Holden
2 years
Bacteriology twitter: any advice on sterile technique at the bench without a bunsen burner? I've heard people do it quite successfully and bunsens are frowned on in our new labs
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@seamus_holden
Séamus Holden
3 years
Bacterial cell division & bacterial microscopy must-read. It's going to take me a little while to parse the biological findings, but simply, these are the highest resolution fluorescence (STORM) images of bacterial cell wall synthesis I seen to date
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@seamus_holden
Séamus Holden
11 months
Up to 8 year postdoc and technician positions will be available in my lab on this topic - if interested email/ DM me
@seamus_holden
Séamus Holden
11 months
We just got a @wellcometrust Discovery Award to work with @SaricLab and Jan Löwe's labs on the mechanistic principles of bacterial cell division! So excited to work with these amazing people on this important and fascinating problem!
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@seamus_holden
Séamus Holden
3 years
Big day - first ever PhD viva of a Holden lab student! Good luck @NTregidgo , you will crush it!
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@seamus_holden
Séamus Holden
6 years
Final #bacell2018 tweet: Well done to Holden lab members @SciYann & Calum Jukes for this #bacell2018 photo competition winner!
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@seamus_holden
Séamus Holden
4 years
To be frank, #postdocappreciationweek feels hollow until we seriously address academic precarity. The current system is a meat grinder, and an inequitable one at that.
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@seamus_holden
Séamus Holden
3 months
🚨JOBS🚨I've reopened applications for this 4 year PDRA post until 6th March. Would suit broad range of microscopists - eg single molecule tracking, SIM, STORM, expansion microscopy or quantitative single cell microscopy. Apply here:
@seamus_holden
Séamus Holden
4 months
Post 1: Postdoctoral researcher in cellular super-resolution microscopy. Looking for super-res expert to resolve nanoscale principles of bacterial cell division. Fun, impactful, challenging SR imaging problem. No bacteriology experience required!
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@seamus_holden
Séamus Holden
6 years
This week we are moving our single molecule microscope, Theya, to a new home in the basement (with space for a sibling) 😄. So until we get the optical table moved I've got some very expensive office decorations
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@seamus_holden
Séamus Holden
2 years
Excited to present a detailed protocols paper by @KevinDWhitley and co-authors about our VerCINI method for vertical bacterial cell immobilization and high resolution imaging. This paper should help make it easily accessible to other labs. Tweetorial below.
@KevinDWhitley
Kevin Whitley
2 years
Our newest paper is out in @NatureProtocols : ! This is a practical guide for interested labs to implement two powerful new methods for high-resolution bacterial microscopy 🧫🔬. (art here 👇by @LizahvdAart )
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@seamus_holden
Séamus Holden
5 years
Realised it was time to change my lab-coated profile photo when I picked up a pipette in the lab and two lab members literally started laughing at me.
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@seamus_holden
Séamus Holden
3 years
We are looking to hire a permanent advanced imaging and image analysis staff scientist. Flexible start dates. Junior (just finishing PhD/ 1st postdoc) or senior scientist applications welcome. Closing Date: 24 June 2021. Apply here: 1/5
@seamus_holden
Séamus Holden
3 years
🚨😀Cool job alert😀🚨Excited to announce a _permanent_ quantitative microscopy staff scientist position at the Centre for Bacterial Cell Biology to collaborate on advanced microscopy and quantitative image processing with world class bacteriology labs 1/7
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@seamus_holden
Séamus Holden
3 years
ZOMG, @eleni_karinou is moving on to new pastures - we will miss you! - and commissioned a Holden lab portrait from @LizahvdAart . This is the coolest thing, incredibly kind and thoughtful!
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@seamus_holden
Séamus Holden
6 years
I wrote a review about bacterial cell division and fancy microscopy!
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@seamus_holden
Séamus Holden
2 years
All our equipment is now safely delivered to @WarwickLifeSci . Now time to go camping for a week before starting the exciting new adventure 😀
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@seamus_holden
Séamus Holden
2 years
I am on strike today to support better working conditions for my precariously employed, underpaid, unequally treated colleagues. I have seen first hand these issues drive talented less privileged individuals out of academia. This is a massive waste #UCUstrike #OneOfUsAllOfUs 1/2
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@seamus_holden
Séamus Holden
5 years
Paper accepted! Wonderful, massively international collaboration with friends and colleagues from all over Europe and rest of the world. Really looking forward to this whole "feck everyone else" Brexit thing then. I'm sure it won't cause UK science to implode.
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@seamus_holden
Séamus Holden
3 years
After hard work by PhD students Mia Conduit and @JEdwards262 , and with nice tubulin samples from @abbelight we now have good quality dSTORM up and running in the lab🎉. Next stop bacteria
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@seamus_holden
Séamus Holden
3 years
Pleased to make our open single molecule microscopy LifeHack platform publicly available. Great work led by @JEdwards262
@JEdwards262
Josh Edwards
3 years
Exciting to have my first ever tweet be announcing a really cool project that I’ve been working on, the LifeHack microscope. It’s an open-source, modular, live cell and single molecule microscope with full CAD models and extensive documentation available from its website.
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@seamus_holden
Séamus Holden
2 years
Grant application submitted. This one hurt! Fingers of other hand crossed it will be worth it 🤣
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@seamus_holden
Séamus Holden
2 years
I will shortly be advertising a PDRA position at @WarwickLifeSci on single molecule fluorescence microscopy of bacterial cell division. Would suit either single molecule biophysicists or molecular microbiologists. Happy to discuss informally.
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@seamus_holden
Séamus Holden
3 years
On Friday I thought I just had "the worst cold ever", and was even negative by LFT, but to be cautious I self-isolated - skipping an in-person departmental seminar - and got a PCR test. It was positive. If in doubt, get tested!
@unibirm_MDS
Medical and Dental Research (UoB)
3 years
There has been talk of 'the worst cold ever'. Prof Alan McNally ( @alanmcn1 ) – who was Infectious Disease lead at the Milton Keynes @LhouseLabsUK – comments on the need to continue COVID-19 testing, as symptoms are similar 👇
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@seamus_holden
Séamus Holden
2 years
Behind the smile and banana respectively is seething anger at university employers #UCUstrike @NewcastleUniUCU @ucu
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@seamus_holden
Séamus Holden
6 years
Our preprint on assessment of super-resolution microscopy software, including the first ever broad assessment of 3D SMLM software out. Big community driven effort, > 30 teams, a lot of labour-of-love work from many labs. Really happy to see this out. Feedback welcome!
@biorxivpreprint
bioRxiv
6 years
Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software #bioRxiv
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@seamus_holden
Séamus Holden
4 years
There is a lot that sucks about virtual science conferences, especially way poorer networking. But to be honest all of that is outweighed by getting to walk out of my office at the end of each conference day and hang out with my kids
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@seamus_holden
Séamus Holden
5 years
overall, especially in the early days pre-Holden lab of now 7 people (😀!), it just made the whole tenure track experience less lonely and much more fun. 10/10 would recommend to any scientist. 4/4
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@seamus_holden
Séamus Holden
2 years
T-1 until vaction: paper accepted 🎉
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@seamus_holden
Séamus Holden
3 years
One bit of good news before I sign off: Yesterday, my sister, an incredible pediatrician in the NHS, received her first dose of the Pfizer COVID vaccine as a frontline healthcare worker. Really feels like we're at the turning point on this thing. Merry Christmas!
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@seamus_holden
Séamus Holden
2 years
What a great sci-comm video about basic bacteriology! So often it is hard to get a press office to publicise work that is important and exciting but not directly translational, and this is a fantastic example of how to do that well.
@harvardmed
Harvard Medical School
2 years
Researchers provide never-before-seen views of double-membraned bacteria as they divide
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@seamus_holden
Séamus Holden
2 years
Packing up the first microscope we built at Newcastle for our lab move is bittersweet. Lots of good times and some useful discoveries.
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@seamus_holden
Séamus Holden
1 year
2 year PDRA position in my lab at Warwick. Looking for an in vitro single molecule FRET expert to apply their skills to study bacterial cell wall synthesis proteins. We offer relocation allowance and cover visa+NHS immigration charges. Flexible start.
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@seamus_holden
Séamus Holden
5 months
Save the date for this great meeting - speaker line-up out soon!
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@seamus_holden
Séamus Holden
5 years
A lot of PIs say they found it hard to lose the first hand thrill of discovery when stepping away from the bench.I've actually found it even more exciting when one of my team turns up with a beautiful result. I think because of the pride in their success as well as the sweet data
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Séamus Holden
8 months
We are recruiting an 8 year senior research technician with a strong background in molecular biology. Details here: Application deadline: 26 September 2023.
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@seamus_holden
Séamus Holden
4 years
Brought the little fella to the @NewcastleUniUCU @DurhamUCU #UCUstrike rally today. He loved it - he was shouting and clapping along to the speeches in an appropriately righteous fashion
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@seamus_holden
Séamus Holden
3 years
Vaccinated 😄 As part of Valneva phase III trial. Cool study - first inactivated virus vaccine trial in Europe.
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@seamus_holden
Séamus Holden
3 years
The person hired for this staff scientist position has bailed at the last minute - very frustrating. But for microscopy geeks, it has the benefit that we are looking for someone to join our team. Informal enquiries, please get in touch
@seamus_holden
Séamus Holden
3 years
We are still looking for an outstanding person for this really exciting permanent staff scientist post and have just readvertised here: Deadline 10 March 2021. Note increased starting salary - was originally advertised a bit lower due to miscommunication
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@seamus_holden
Séamus Holden
7 years
Ooh I have 1000 followers! Hope everyone likes the random mix of microbiology and fancy microscopy!
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@seamus_holden
Séamus Holden
3 months
1 week left to apply for 4 year post-doc jobs in my lab! Happy to chat informally to interested candidates. If you like sound of the posts but are not sure you fit perfectly - apply anyway! - or happy to discuss.
@seamus_holden
Séamus Holden
4 months
🚨JOBS🚨 Two 4 year post-docs available in my lab at Warwick. Looking for experts in super-resolution microscopy, single molecule fluorescence, FRET or protein biochemistry. Visa+NHS fees covered. Salary~£40K. Two exciting team science projects with outstanding collaborators
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@seamus_holden
Séamus Holden
4 years
What separates DNA after replication so that growing bacteria each contain at least one chromosome? In work led by @Errington_Lab we found that the spacing of bacterial nuclear material is set by cell volume in cell wall free L-form bacteria
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@seamus_holden
Séamus Holden
2 years
Join us! Looking for an in vitro single molecule fluorescence spectroscopy/ smFRET expert to join our team and apply their skills to study bacterial cell wall synthesis proteins. I am so pysched about this project, DM/ email me to find out more!
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@seamus_holden
Séamus Holden
4 years
Ive been wearing my @Thorlabs tshirt around the house because I miss the lab, and my youngest son fell in love with the Thor doggy. Thanks a million to @Thorlabs UK for sending him one of his very own!
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@seamus_holden
Séamus Holden
3 years
Join the team! PhD project on offer in my lab. Really nice quantitative microscopy & biophysics project. Collaboration with @HenriquesLab and Sharples lab, Durham. Looking for student with Physics/ CompSci/ related training. Deadline 22 Jan 2021.
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@seamus_holden
Séamus Holden
3 years
Very well done @JukesCalum from my lab on passing his PhD viva with minor corrections! 🍾😀🥂🍻 And huge thanks to @nartimsoole and Heath Murray for examining.
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@seamus_holden
Séamus Holden
4 years
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@seamus_holden
Séamus Holden
7 months
If you would like to do a 5 year post-doc on an ambitious team science project with fantastic collaborators, get in touch! You will focus on using in vitro single molecule FRET to study bacterial cell wall remodelling. @pstansfeld + @davidroper2 have positions too. Adverts soon.
@davidroper2
David I Roper
7 months
A little something utterly awesome happened for me @pstansfeld @seamus_holden & @Cochrane_lab . Job adds being drafted now for 3PDRAs and a Graduate RA @WarwickLifeSci
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Séamus Holden
6 years
Really chuffed - a really fantastic student just won a BBSRC PhD studentship in my lab. Roll on the next academic year!
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@seamus_holden
Séamus Holden
5 years
@FOMconferences @SuperResoluSian @gailmcconnell @SusanSuperRes @FOMconferences you will notice that the line up of both MMC and ELMI are both reasonably gender balanced. It's not hard - our field has many outstanding female scientists.
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@seamus_holden
Séamus Holden
4 years
A delightful conversation about deep learning and pix2pix yesterday with @HenriquesLab and @miCHRIScopy let me to create this monstrosity (you can make your own here )
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@seamus_holden
Séamus Holden
2 years
People are rightly complaining about recent bad experiences with a large virtual society conference. But I'm convinced smaller virtual conferences can be amazing, and massively increase global access.Subtillery - virtual Bacillus conference started by @Eswara_Lab - case in point
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@seamus_holden
Séamus Holden
6 years
Fully funded UK/EU PhD studentship in advanced microscopy & quantitative biology. Push the limits of super-resolution microscopy, build fancy microscopes, take beautiful pictures, reveal how bacteria grow and divide. Apply here:
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@seamus_holden
Séamus Holden
4 years
Updating my cell division/ cell wall biophysics lectures, and it's a wonderful chance to reread @Laia_Pasquina 's 2020 cell wall ultrastructure paper, this has got to be one of my favourite figures in a paper ever ()
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