Scientific highlight of last year:
Last month I used our lab's new 3D printer to create a replacement wheel for my 4 year old's broken Transformer. When I presented it to him, he solemnly told me "Daddy, you are a great scientist".
Nobel winners, eat your heart out.
Exciting news about a new adventure: I and most of the Holden lab are moving to
@warwickuni
@WarwickLifeSci
new Interdisciplinary Biosciences Research Building in spring next year! And a promotion to Associate Professor (Reader). PhD and PDRA position available - details soon
We just got a
@wellcometrust
Discovery Award to work with
@SaricLab
and Jan Löwe's labs on the mechanistic principles of bacterial cell division! So excited to work with these amazing people on this important and fascinating problem!
Delighted to announce
@HenrikStrahl
, Waldemar Vollmer, and myself have been awarded a
@BBSRC
grant to investigate mechanistic principles of bacterial cell wall construction by the elongasome!
Our second child arrived this morning, he and his mummy are doing great! And the NHS staff at Newcastle Royal Victoria Infirmary were incredible. Signing off Twitter for a little bit, see you all soon.
So happy our paper using badass microscopy to figure out the function(s) of FtsZ treadmilling in Bacillus subtilis cell division is out! Immensely proud of the work of all authors on this study over the last 4 years, in face of obvious global challenges.
My little boys (possibly+mum) made me an awesome bacillus birthday cake! 6yo made the marzipan rods with division septa. Microscale bacilli sprinkles courtesy of 2yo.
I have a 3 year post doc position available in my lab at
@WarwickLifeSci
. Focus is on bioimage analysis of bacterial single molecule tracking and super-resolution microscopy data. Application deadline is 10th September.
🚨JOBS🚨 Two 4 year post-docs available in my lab at Warwick. Looking for experts in super-resolution microscopy, single molecule fluorescence, FRET or protein biochemistry. Visa+NHS fees covered. Salary~£40K. Two exciting team science projects with outstanding collaborators
Really excited to share this with the world. We used ultra-sensitive custom microscopy to figure out why cytoskeletal filament motility is so important for bacterial cell division. Aka - why are bacterial protein sticks wriggly?
Dear Twitter/ scitwitter, at 2k followers, I paused to reflect on this whole scientific social media thing. I must say, I have got more out of this than I ever expected. I started a Twitter account when I set up my lab, and I thought it would just be a way to find some papers 1/4
In work led by
@KevinDWhitley
, we demonstrate that septal cell wall synthesis in Bacillus subtilis follows a one-track model, where both the motion and activity of the septal PG synthesis machinery is asynchronous with FtsZ dynamics
Huge thanks
@BritBiophysSoc
for this year's BBS Early Career Award! And sincere deep gratitude to all Holden lab members past and present for your passion and hard work.
Importance announcement: BBS prizes! All three prize winners will be presenting at the biennial meeting. Our sincere congratulations to John Christodoulou, Seamus Holden
@seamus_holden
and Daniel Hurdiss. Details are below.
You know what gets my goat? The relentless drive towards certainty in science, especially paper discussion sections. What's wrong with good old doubt, and frankly discussing uncertainties in your data and your model?
So much scientific software dies when the authors abandon it. Believe me I understand the constraints that cause authors to stop actively developing/ supporting software - it all comes down to lack of funding mechanism. But boy does it drive me crazy
Dressed up for
@lmr_1994
's Zoom viva today. Doctoral examinations are almost the only time I wear a suit professionally, out of respect for the many years of work of the candidate. I see no reason to change this for some dumb virus!
This is what 130,000 gloves look like! +95 safety glasses, 3 face shields, 170 disposable surgical face masks, 8 coveralls . To be collected Monday by Newcastle Gateshead CCG for frontline GPs, dentists, hospitals. Thank you
@UniofNewcastle
scientists!!
#getPPE_NCL
I still have 2 4-year post doc jobs available, will advertise early January. One is for a super resolution microscopist to image bacterial cell division, other is for biochemist or single molecule biophysicist to use single molecule FRET to study bacterial cell wall synthesis
🚨😀Cool job alert😀🚨Excited to announce a _permanent_ quantitative microscopy staff scientist position at the Centre for Bacterial Cell Biology to collaborate on advanced microscopy and quantitative image processing with world class bacteriology labs 1/7
We are recruiting 4 Assistant/ Associate Professor positions in microbiology at
@WarwickLifeSci
!
2 on Host-virus interactions, 1 on Plant virology, 1 on Host responses to microbes.
. Please RT!
In work led by Ling Wu and
@Errington_Lab
,we investigated how physical confinement affects cell wall free bacteria. My favourite finding:all you need for robust chromosome segregation is physical confinement in a narrow channel (left vs right panel),
I am readvertising this exciting super-resolution/ single molecule post-doc position, with two major changes. Start date flexible. Prior bacteriology expertise not required.
1) Post is now for 4 years - rationale below
1/2
We are recruiting a post-doctoral researcher in experimental biophysics and super-resolution microscopy. Post is for 5 years, extendible up to a total of 8 years. Details here: . Application deadline: 10 October 2023
Good news: we got a
@UKRI_News
21ALERT grant for a
@zeiss_micro
Lattice SIM2 with FRAP & photoactivation at
@WarwickLifeSci
! This will allow us to establish live cell SIM at Warwick in a range of organisms from bacteria all the way to plants 1/2
We are recruiting a post-doctoral researcher in experimental biophysics and super-resolution microscopy. Post is for 5 years, extendible up to a total of 8 years. Details here: . Application deadline: 10 October 2023
We found that B subtilis divisome synthesis complexes are not coupled to treadmilling FtsZ filaments in either their active or inactive states. Rather, divisome processive motion around septum is exclusively driven by cell wall synthesis
We've just completed the latest updates to the 3D super resolution challenge. Lots of cool new additions, especially analysis on real 3D data, and web tools for interactive data exploration
How I feel about
#UCUstrikes
and
@UCU
right now: Angry and demoralized, and that a lot of mine and everyone else's time, money and effort in strike action has been compromised through poor leadship, poor strategy and a fairly disfunctional union. 1/4
Post-doc job: looking for an awesome bacterial cell biologist/ cell division/ cytoskeleton person to join my lab to unravel organization of bacterial division machinery with super-res microscopy. Super-res expertise not required - we will teach you!
If you are interested in Bacillus subtilis and related organisms, join us for Subtillery, June 8-12, a virtual conference organized by
@Eswara_Lab
. We have amazing speakers, almost all PDRAs and PhDs, from outstanding labs all over the world!
Moving day! Sad to leave all our Newcastle friends but excited to move into a gorgeous new home and not have to do 2000 miles of driving and AirBnB again next month😀
In an entirely remote research project,
@NewcastleMedSch
undergrad members of our lab managed to do bacteriology in their bedrooms with a £30 microscope objective and a DIY
@OpenFlexure
microscope. How cool is that?!
My sister is a frontline NHS doctor and I had to post her my lab's face shield and eyewear because her hospital has nothing to issue her with. And these psychopaths talk about people's feelings being hurt. Doctors and nurses are literally dying from their logistics failure.
Bacteriology twitter: any advice on sterile technique at the bench without a bunsen burner? I've heard people do it quite successfully and bunsens are frowned on in our new labs
Bacterial cell division & bacterial microscopy must-read. It's going to take me a little while to parse the biological findings, but simply, these are the highest resolution fluorescence (STORM) images of bacterial cell wall synthesis I seen to date
We just got a
@wellcometrust
Discovery Award to work with
@SaricLab
and Jan Löwe's labs on the mechanistic principles of bacterial cell division! So excited to work with these amazing people on this important and fascinating problem!
To be frank,
#postdocappreciationweek
feels hollow until we seriously address academic precarity. The current system is a meat grinder, and an inequitable one at that.
🚨JOBS🚨I've reopened applications for this 4 year PDRA post until 6th March. Would suit broad range of microscopists - eg single molecule tracking, SIM, STORM, expansion microscopy or quantitative single cell microscopy. Apply here:
Post 1: Postdoctoral researcher in cellular super-resolution microscopy. Looking for super-res expert to resolve nanoscale principles of bacterial cell division. Fun, impactful, challenging SR imaging problem. No bacteriology experience required!
This week we are moving our single molecule microscope, Theya, to a new home in the basement (with space for a sibling) 😄. So until we get the optical table moved I've got some very expensive office decorations
Excited to present a detailed protocols paper by
@KevinDWhitley
and co-authors about our VerCINI method for vertical bacterial cell immobilization and high resolution imaging. This paper should help make it easily accessible to other labs. Tweetorial below.
Our newest paper is out in
@NatureProtocols
: ! This is a practical guide for interested labs to implement two powerful new methods for high-resolution bacterial microscopy 🧫🔬. (art here 👇by
@LizahvdAart
)
We are looking to hire a permanent advanced imaging and image analysis staff scientist. Flexible start dates. Junior (just finishing PhD/ 1st postdoc) or senior scientist applications welcome. Closing Date: 24 June 2021. Apply here: 1/5
🚨😀Cool job alert😀🚨Excited to announce a _permanent_ quantitative microscopy staff scientist position at the Centre for Bacterial Cell Biology to collaborate on advanced microscopy and quantitative image processing with world class bacteriology labs 1/7
ZOMG,
@eleni_karinou
is moving on to new pastures - we will miss you! - and commissioned a Holden lab portrait from
@LizahvdAart
. This is the coolest thing, incredibly kind and thoughtful!
I am on strike today to support better working conditions for my precariously employed, underpaid, unequally treated colleagues. I have seen first hand these issues drive talented less privileged individuals out of academia. This is a massive waste
#UCUstrike
#OneOfUsAllOfUs
1/2
Paper accepted! Wonderful, massively international collaboration with friends and colleagues from all over Europe and rest of the world.
Really looking forward to this whole "feck everyone else" Brexit thing then. I'm sure it won't cause UK science to implode.
After hard work by PhD students Mia Conduit and
@JEdwards262
, and with nice tubulin samples from
@abbelight
we now have good quality dSTORM up and running in the lab🎉. Next stop bacteria
Exciting to have my first ever tweet be announcing a really cool project that I’ve been working on, the LifeHack microscope.
It’s an open-source, modular, live cell and single molecule microscope with full CAD models and extensive documentation available from its website.
I will shortly be advertising a PDRA position at
@WarwickLifeSci
on single molecule fluorescence microscopy of bacterial cell division. Would suit either single molecule biophysicists or molecular microbiologists. Happy to discuss informally.
On Friday I thought I just had "the worst cold ever", and was even negative by LFT, but to be cautious I self-isolated - skipping an in-person departmental seminar - and got a PCR test. It was positive. If in doubt, get tested!
There has been talk of 'the worst cold ever'. Prof Alan McNally (
@alanmcn1
) – who was Infectious Disease lead at the Milton Keynes
@LhouseLabsUK
– comments on the need to continue COVID-19 testing, as symptoms are similar 👇
Our preprint on assessment of super-resolution microscopy software, including the first ever broad assessment of 3D SMLM software out. Big community driven effort, > 30 teams, a lot of labour-of-love work from many labs. Really happy to see this out. Feedback welcome!
Exciting open microscopy development PhD project available in my lab at
@WarwickLifeSci
. Application deadline 9th January. Fully funded position, open to UK/ EU/ international students
There is a lot that sucks about virtual science conferences, especially way poorer networking. But to be honest all of that is outweighed by getting to walk out of my office at the end of each conference day and hang out with my kids
overall, especially in the early days pre-Holden lab of now 7 people (😀!), it just made the whole tenure track experience less lonely and much more fun. 10/10 would recommend to any scientist. 4/4
One bit of good news before I sign off: Yesterday, my sister, an incredible pediatrician in the NHS, received her first dose of the Pfizer COVID vaccine as a frontline healthcare worker. Really feels like we're at the turning point on this thing. Merry Christmas!
What a great sci-comm video about basic bacteriology! So often it is hard to get a press office to publicise work that is important and exciting but not directly translational, and this is a fantastic example of how to do that well.
2 year PDRA position in my lab at Warwick. Looking for an in vitro single molecule FRET expert to apply their skills to study bacterial cell wall synthesis proteins.
We offer relocation allowance and cover visa+NHS immigration charges. Flexible start.
A lot of PIs say they found it hard to lose the first hand thrill of discovery when stepping away from the bench.I've actually found it even more exciting when one of my team turns up with a beautiful result. I think because of the pride in their success as well as the sweet data
We are recruiting an 8 year senior research technician with a strong background in molecular biology. Details here: Application deadline: 26 September 2023.
Brought the little fella to the
@NewcastleUniUCU
@DurhamUCU
#UCUstrike
rally today. He loved it - he was shouting and clapping along to the speeches in an appropriately righteous fashion
The person hired for this staff scientist position has bailed at the last minute - very frustrating. But for microscopy geeks, it has the benefit that we are looking for someone to join our team. Informal enquiries, please get in touch
We are still looking for an outstanding person for this really exciting permanent staff scientist post and have just readvertised here: Deadline 10 March 2021. Note increased starting salary - was originally advertised a bit lower due to miscommunication
1 week left to apply for 4 year post-doc jobs in my lab! Happy to chat informally to interested candidates. If you like sound of the posts but are not sure you fit perfectly - apply anyway! - or happy to discuss.
🚨JOBS🚨 Two 4 year post-docs available in my lab at Warwick. Looking for experts in super-resolution microscopy, single molecule fluorescence, FRET or protein biochemistry. Visa+NHS fees covered. Salary~£40K. Two exciting team science projects with outstanding collaborators
What separates DNA after replication so that growing bacteria each contain at least one chromosome? In work led by
@Errington_Lab
we found that the spacing of bacterial nuclear material is set by cell volume in cell wall free L-form bacteria
Join us! Looking for an in vitro single molecule fluorescence spectroscopy/ smFRET expert to join our team and apply their skills to study bacterial cell wall synthesis proteins. I am so pysched about this project, DM/ email me to find out more!
Ive been wearing my
@Thorlabs
tshirt around the house because I miss the lab, and my youngest son fell in love with the Thor doggy. Thanks a million to
@Thorlabs
UK for sending him one of his very own!
Join the team! PhD project on offer in my lab. Really nice quantitative microscopy & biophysics project. Collaboration with
@HenriquesLab
and Sharples lab, Durham. Looking for student with Physics/ CompSci/ related training. Deadline 22 Jan 2021.
Very well done
@JukesCalum
from my lab on passing his PhD viva with minor corrections! 🍾😀🥂🍻 And huge thanks to
@nartimsoole
and Heath Murray for examining.
If you would like to do a 5 year post-doc on an ambitious team science project with fantastic collaborators, get in touch! You will focus on using in vitro single molecule FRET to study bacterial cell wall remodelling.
@pstansfeld
+
@davidroper2
have positions too. Adverts soon.
A delightful conversation about deep learning and pix2pix yesterday with
@HenriquesLab
and
@miCHRIScopy
let me to create this monstrosity (you can make your own here )
People are rightly complaining about recent bad experiences with a large virtual society conference. But I'm convinced smaller virtual conferences can be amazing, and massively increase global access.Subtillery
- virtual Bacillus conference started by
@Eswara_Lab
- case in point
Updating my cell division/ cell wall biophysics lectures, and it's a wonderful chance to reread
@Laia_Pasquina
's 2020 cell wall ultrastructure paper, this has got to be one of my favourite figures in a paper ever ()