Ruth Hook @ruth_hook_ Twitter profile | Pikagi

Pikagi

Ruth Hook

@ruth_hook_

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1,128

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immunology & stem cell biology- tissue culture in startups and basic research 🇺🇸🇮🇪🇫🇮 citizen- reading, running, climbing, paleontology, folk harp etc

NYC

https://t.co/LRFwLCOUtW

Joined October 2015

Don't wanna be here? Send us removal request.

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@ruth_hook_

Ruth Hook

5 months

has there ever been a problem in science where the solution hasn't been "it's far more nuanced, complex, & context dependent than we thought" just once it would be nice for overwhelming complexity to suddenly simplify itself with additional understanding from a new discovery

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@ruth_hook_

Ruth Hook

1 year

actual emails from my father

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@ruth_hook_

Ruth Hook

2 months

I know renting is essentially setting money on fire but every time I hang out with a friend who recently bought a house it reminds me that home ownership is really the modern form of adam & eve eating from the tree of knowledge and falling from grace

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@ruth_hook_

Ruth Hook

1 year

it's a well-kept secret but the actual reason people get married & make fewer friends in their late 20s and early 30s because a decade of adulthood is the point where the overall narrative gets too convoluted to explain to new people

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@ruth_hook_

Ruth Hook

1 month

what are examples of eerie unexpected heritability in your life if you're privileged enough to have a good relationship with your family mine was finding out my dad and I ate the exact same thing during undergrad all nighters without ever discussing it & they're v odd choices

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@ruth_hook_

Ruth Hook

10 months

so what are everyone's theories

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@ruth_hook_

Ruth Hook

1 year

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@ruth_hook_

Ruth Hook

1 year

also if you ever need a paper behind a paywall that's not on scihub- dm me- I have journal access, a family of NAS professors who raised me to hate elsevier, & a friend circle of researchers who all support open science- am more than happy to be captain hook the science pirate

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@ruth_hook_

Ruth Hook

2 months

having a normal one

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@ruth_hook_

Ruth Hook

1 year

did y'all know NASA was legitimately worried that the moon wasn't going to have a hard surface to land on and decided to just go for it due to the similarities between observed topography and the valles caldera

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@ruth_hook_

Ruth Hook

1 year

poor man's ozempic (running)

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@ruth_hook_

Ruth Hook

1 year

are c-sections unnatural or is it actually really cool that our species came up with a solution for our big heads using our big heads (can already feel the maternal microbiome ppl coming for me w/pitchforks)

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@ruth_hook_

Ruth Hook

11 months

not to be cliché, but does anyone have any advice for your 30s

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@ruth_hook_

Ruth Hook

1 year

we are no longer making fun of how buyable/cheap Forbes 30u30 is after finding out it's mainly for o1 visas

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@ruth_hook_

Ruth Hook

6 months

ever since I learned sharks are older than the north star, I've been trying to figure out exactly when in earth's history the north star appeared in our sky with no avail just to see if it coincided with anything cool in evolution this is 300% not scientific, I just want to know

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@ruth_hook_

Ruth Hook

1 year

this is still maybe my favorite thing that ever happened on twitter

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@ruth_hook_

Ruth Hook

1 month

I do not for a second expect that that is actually all that heritable I just want people's creepy coincidence stories

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@ruth_hook_

Ruth Hook

1 year

how wild is it that so much of our biochemistry fluctuates with the seasons which we only have due to a giant rock slamming into us billions of years ago to create the moon

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@ruth_hook_

Ruth Hook

1 month

@creid815 this is exactly what I was looking for ty

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@ruth_hook_

Ruth Hook

1 year

I still can't believe we don't teach biology primarily as history- like my brain kind of broke when I realized HMGB1 is something we have from when we were archaea

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@ruth_hook_

Ruth Hook

1 year

imho one of the most significant findings of last year

@FlurinCathomas

Flurin Cathomas

@FlurinCathomas

1 year

Your brain could be controlling how sick you get — and how you recover

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@ruth_hook_

Ruth Hook

10 months

one time I took 25mgs of zinc for a month just to prove to my GP that iron and zinc do in fact share the exact same absorption & storage pathways and it's kind of insane to see all the anti-iron rhetoric right now in the public without mention of this

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@ruth_hook_

Ruth Hook

2 months

do ecosystems need mosquitoes or ticks it's a no right

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@ruth_hook_

Ruth Hook

5 months

this is where we get ozempic from btw

@IhabFathiSulima

Dr Ihab Suliman. دكتور ايهاب فتحي سليمان

@IhabFathiSulima

5 months

The saliva of this animal Lizard proved to be very useful in clinical medicine. Please Explain?

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@ruth_hook_

Ruth Hook

1 year

not enough people appreciate that their skin and brain are derived from the same developmental layer neuroectoderm

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@ruth_hook_

Ruth Hook

4 months

what's on your reading list for 2024

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@ruth_hook_

Ruth Hook

5 months

expected 0 answers & unintentionally got a comment section full of uplifting ones that give hope I feel like the discovery of AIRE too would also qualify

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@ruth_hook_

Ruth Hook

1 year

I was in fact 4' 11" back then and I moved about the country with all of my belongings in just that backpack

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@DestiniYvette

Dess 💕

1 year

Let me see yall at 19? 👀

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@ruth_hook_

Ruth Hook

1 year

in the UK when a wealthy woman schedules an elective c-section ahead of time it's called "too posh to push"

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@ruth_hook_

Ruth Hook

1 year

@MasterTimBlais hi it's me tl/dr: company was selling cells as induced pluripotent stem cells (reprogrammed somatic cell) when in reality they had a contamination and were selling embryonic stem cells

@ruth_hook_

Ruth Hook

1 year

the ncardia cell contamination scandal- so CDI (in the v red until fujifilm bought) sells a product called iCells cardiomyocytes that are iPSCs diffed into CMs and sold at a super high price and theoretically you can use them to make cardiac constructs (1/n)

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@ruth_hook_

Ruth Hook

1 year

as the child of a greencard marriage, I support all paths but mainly the most efficient ones

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@ruth_hook_

Ruth Hook

9 months

I deleted most of my spicy tweets to make this account more professional and every day I regret it more and more anyway, there are none/fund basic research

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@ruth_hook_

Ruth Hook

1 year

roses are red, violets are blue, gardasil prevents cancer, & men should get it too

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@ruth_hook_

Ruth Hook

10 months

I've heard colloquially that there's something in development that can turn on that makes the likelihood of being L vs R handed 50/50 and I know nothing else

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@ruth_hook_

Ruth Hook

2 months

what's your least favorite part of your job (genuinely curious to see how frequently they're the same)

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@ruth_hook_

Ruth Hook

1 month

@jasoncbenn @bryan_johnson oh that candidate gene study failed to replicate anecdotally I'm homozygous for the "short sleep" version of dec2/rs121912617 and usually fully need an 8 hours

The impact of Mendelian sleep and circadian genetic variants in a population setting - PubMed

Rare variants in ten genes have been reported to cause Mendelian sleep conditions characterised by extreme sleep duration or timing. These include familial natural short sleep (ADRB1, DEC2/BHLHE41,...

pubmed.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

10 months

oh crucial context being I'm a c282y hfe homozygote and my ferritin and TF% shot back up anyway biochemists who name proteins after specific metals when they really aren't selective sort of nerf a lot of fields

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@ruth_hook_

Ruth Hook

1 year

432 vs 440 hz (in comments) @creatine_cycle

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@ruth_hook_

Ruth Hook

10 days

it's saturday night, time to install R

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@ruth_hook_

Ruth Hook

1 year

there was like two days where everyone was giving wild answers as to what the first cause could be before the cdc corrected it

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@ruth_hook_

Ruth Hook

1 year

440 Hz

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@ruth_hook_

Ruth Hook

1 month

@MacGoogly interesting- someone else mentioned cucumber and I think they're both high in l-citrulline

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@ruth_hook_

Ruth Hook

1 year

@MasterTimBlais I'm going to write a thread on this and then link it here

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@ruth_hook_

Ruth Hook

1 year

does it spark panic

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@ruth_hook_

Ruth Hook

1 year

@MasterTimBlais I have so much more like 7/10 lung cancer cell lines have iron regulation mutations, K562s express embryonic zeta globin, people using bone marrow cancer lines KNOWN for donating mitochondria reparatively as cardiac fibroblasts, and using HeLa cells in a paper = astrology

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@ruth_hook_

Ruth Hook

1 year

the ncardia cell contamination scandal- so CDI (in the v red until fujifilm bought) sells a product called iCells cardiomyocytes that are iPSCs diffed into CMs and sold at a super high price and theoretically you can use them to make cardiac constructs (1/n)

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@ruth_hook_

Ruth Hook

5 months

@sebatlab [2024] I've hated science since being taught introns are junk DNA, viruses aren't alive, and western blots are considered quantitative

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@ruth_hook_

Ruth Hook

6 months

maybe I've been reading too many IL-17 papers but what if we just stopped using anti/pro inflammatory as descriptors for cytokines

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@ruth_hook_

Ruth Hook

1 year

the cilia of your respiratory tract are pretty much identical to the cilia of your reproductive tract- evolutionarily where do you predict it first arose

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@ruth_hook_

Ruth Hook

10 months

@meatballtimes does China force kids to be right handed?

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@ruth_hook_

Ruth Hook

1 year

@MasterTimBlais none of this was related to contamination which also runs amok because EVERYONE SHOULD BE DOING CONFIRMATORY SEQUENCING ALWAYS Merrick is great and you should all subscribe to his substack

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Stem cells are out to get me

Some hidden pitfalls of stem cell research, and how to avoid them

denovo.substack.com

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@ruth_hook_

Ruth Hook

1 year

@SexyLikeMeiosis lol My favorite abstract from ASGCT this year

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@ruth_hook_

Ruth Hook

1 year

I'm sorry but there is nothing as good as path twitter bored on a first date? path twitter preparing for parenthood? path twitter bored waiting to finish staining? path twitter shutting down the cytometer? path twitter etc

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@ruth_hook_

Ruth Hook

1 month

@kapahi_pankaj if they're an actually interested trainee they'll see this and know there's so many great candidates out there I would move on but my mother's foreign postdocs have been using chatgpt to basically bandaid their difficulties with English as a 2nd/3rd language & that would be my…

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@ruth_hook_

Ruth Hook

2 years

@tomkXY @kulesatony Did you know that it's due to a horizontal gene transfer event from arthropods from the Carboniferous?

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An Ancient Transkingdom Horizontal Transfer of Penelope-Like Retroelements from Arthropods to...

Comparative genomics analyses empowered by the wealth of sequenced genomes have revealed numerous instances of horizontal DNA transfers between distantly related species. In eukaryotes, repetitive...

www.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

1 month

I am begging anyone to actually characterize FBS

@mbeisen

Michael Eisen

1 month

Easy to dunk on (and I did) but it’s a generic problem in modern science that people are using not just code, but reagents and methods whose proper use and more importantly limitations they don’t understand and often don’t have any straightforward way to understand.

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@ruth_hook_

Ruth Hook

7 months

does anyone know what type of eagle/falcon this is! pictured at breakneck ridge this weekend in NY

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@ruth_hook_

Ruth Hook

2 months

I was hoping to reach some of the crazy ecology professors on here who could give some fringe side cases and then say no

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@ruth_hook_

Ruth Hook

1 year

do you consider dolphins and whales to be different species and do you consider lions and tigers to be different species

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@ruth_hook_

Ruth Hook

1 month

was there just an earthquake in NYC

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@ruth_hook_

Ruth Hook

1 year

side note fun fact

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Archaeal Ribosomal Proteins Possess Nuclear Localization Signal-Type Motifs: Implications for the...

Eukaryotic cells are divided into the nucleus and the cytosol, and, to enter the nucleus, proteins typically possess short signal sequences, known as nuclear localization signals (NLSs). Although...

www.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

1 year

dm'd to me half an hour ago

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Severe chronic psychosis after allogeneic SCT from a schizophrenic sibling

www.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

2 years

@chrstn_e Everyone knows but dinoflagellates and histones/nucleosomes!

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Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net

Scientific Reports - Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net

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@ruth_hook_

Ruth Hook

1 year

fun fact: a human specific gene duplication of SRGAP2A allows antagonism of the original which promotes spine maturation & this allows human pyramidal neurons to have increased spine density, radial migration, & allows emergence of the our neocortex

A human-specific modifier of cortical connectivity and circuit function

Nature - The human-specific gene duplication SRGAP2C is identified as a modifier of structural and functional features of cortical circuits leading to improved behavioural performance that may have...

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@ruth_hook_

Ruth Hook

1 year

fun fact: you have a self-cleaving ribozyme in your genome that determines episodic memory

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Experimental Resurrection of Ancestral Mammalian CPEB3 Ribozymes Reveals Deep Functional Conserva...

Abstract. Self-cleaving ribozymes are genetic elements found in all domains of life, but their evolution remains poorly understood. A ribozyme located in t

academic.oup.com

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@ruth_hook_

Ruth Hook

1 year

tl/dr: these were CMs diffed from Rues2 ESCs not iPSCs moral: sequence everything always, store your cells in the vapor phase w/internally threaded cryovials, label (9/n last)

@NcardiaStemCell

Ncardia

@NcardiaStemCell

5 years

Cor.4U® - fully functional hiPSC-derived cardiomyocytes. Here you can see them beating on a patterned substrate, with visible sarcomeres & mitochondria. View full video at: #Cor4U #cardiomyocytes #safetypharmacology #ncardia #video

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@ruth_hook_

Ruth Hook

1 year

credit to @a_manifolder for giving this to me weeks ago which if you know me irl you know I annoyingly never stop talking about MOR in immunology & neurodevelopment

Seasonal Variation in the Brain μ-Opioid Receptor Availability

Seasonal rhythms influence mood and sociability. The brain μ-opioid receptor (MOR) system modulates a multitude of seasonally varying socioemotional functions, but its seasonal variation remains...

www.jneurosci.org

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@ruth_hook_

Ruth Hook

1 year

what are your wildest theories about eosinophils

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@ruth_hook_

Ruth Hook

7 months

absolute shot in the dark but do we have a follow up on this patient/how her tumors responded if she got a booster just curious sorry

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Spontaneous tumor regression following COVID-19 vaccination

Vaccination against COVID-19 is critical for immuno-compromised individuals, including patients with cancer. Systemic reactogenicity, a manifestation of the innate immune response to vaccines, occurs...

www.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

1 year

do you believe in species

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see results

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@ruth_hook_

Ruth Hook

21 days

does anyone know what SPECIFIC species of worm thrives in the layer of fat & rotting material in bubbly creek in chicago/the remnants of the stockyards of upton sinclairs jungle

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@ruth_hook_

Ruth Hook

5 months

@StearnsLab biotech is super bad right now!!! xbi is low, everyone is getting laid off, private capital investment is low, people are realizing the basic research gaps holding back any downstream technology development & wanting to work on them

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@ruth_hook_

Ruth Hook

6 months

EVs to you mean:

extracellular vesicles

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electric vehicles

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@ruth_hook_

Ruth Hook

1 year

transferrin

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@ruth_hook_

Ruth Hook

1 year

/test drugs which I did and they're industry standard - however a lab in germany ran scRNAseq and found them all to be transcriptionally homogenous with fibroblast markers instead of any atrial vs ventricular markers (2/n)

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@ruth_hook_

Ruth Hook

17 days

what running shoes should I get myself for my birthday current ones look like Muppets characters/are at the end of their life

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@ruth_hook_

Ruth Hook

1 year

Anyway, iCells are stupid expensive & you have to preplate because the initial die off is unacceptably variable & it's all around a scam- just diff your own CMs (Wu lab protocols). nCardia wanted to break up this monopoly and started selling a line of iPSC-CMs called Cor.4U (3/n)

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@ruth_hook_

Ruth Hook

1 year

follow up: were you aware that the offspring for these pairings (whales & dolphins and lions & tigers) are fertile

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@ruth_hook_

Ruth Hook

11 months

@viriditax this was a paper I read before I realized I needed an intervention to stop reading frontiers papers and medical theories

Tick bite induced α‐gal syndrome highlights anticancer effect of allergy

BioEssays is a review-and-discussion journal renowned for its collection of contemporary and forward-looking reviews on molecular, genetic and cellular biology.

onlinelibrary.wiley.com

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@ruth_hook_

Ruth Hook

1 year

around 2017? Anyway, not to start a DFW footnote but in the US there's v strict regulations on use of ESCs vs iPSCs and you have to fill out infinite paperwork for the former & you couldn't get federal $ during the Bush admin (thx obama but actually) & the latter is chill (4/n)

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@ruth_hook_

Ruth Hook

1 year

Do you subscribe to determinism and have you worked with RNA?

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@ruth_hook_

Ruth Hook

6 months

do we know WHY cipro causes tendon issues

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@ruth_hook_

Ruth Hook

1 year

Scientists starting using Cor.4U in cardiac stem cell papers as iPSC-CMs until one day in 2021 someone decided to do confirmatory sequencing on the cells (I actually don't know who did this, if you do lmk)... (5/n)

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@ruth_hook_

Ruth Hook

1 year

When the confirmatory sequecing was done on the iPSC-CMs from nCardia (Cor.4U) - it turned out instead of iPSCs they were actually RUES2 ESCs that had been diffed into CMs and major contamination had happened at some point- instead of iPSCs, everyone was actually using ESCs (7/n)

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@ruth_hook_

Ruth Hook

4 months

@SamuelVernon14 I feel like I didn't truly appreciate that in middle school when we read it for english gerda weissmann klein's all but my life left a much bigger impression on me/still influences my consistent practical footwear choices

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@ruth_hook_

Ruth Hook

1 month

how frequently do you think about endoplasmic reticulum stress response

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@ruth_hook_

Ruth Hook

21 days

@browserdotsys female embryos tend to be favored in ivf

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@ruth_hook_

Ruth Hook

17 days

@manorlaboratory clone, transform dh5alpha magic cells from zymo, confirm sequence, inoculate a mega culture or just a buncho 4 mL cultures, maxiprep with thermo genejet- it's as good as macharey nagel for getting LPS & endotoxins out for downstream txns for making virus so like 2 days and a…

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@ruth_hook_

Ruth Hook

6 months

@evolscientist @mbeisen did you know they inherit their mitochondria from both their mother & father and it's used in their sex determination wait you probably definitely know that

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@ruth_hook_

Ruth Hook

1 month

@eshear increase or decrease

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@ruth_hook_

Ruth Hook

1 year

And it was a scandal and nCardia obviously stopped selling them and any paper that used Cor.4U CMs had to issue corrections/errata clarifying that they were actually RUES2 CMs (8/n) eg:

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Correction: Rehnelt et al. Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by...

www.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

14 days

@ArtirKel does the veillonella finding carry any weight

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@ruth_hook_

Ruth Hook

1 year

almost done with weird true science for the night deep brain photoreceptors!

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Violet-light suppression of thermogenesis by opsin 5 hypothalamic neurons - PubMed

The opsin family of G-protein-coupled receptors are used as light detectors in animals. Opsin 5 (also known as neuropsin or OPN5) is a highly conserved opsin that is sensitive to visible violet...

pubmed.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

2 years

@Steeltown_Worms @chrstn_e And tangentially mistletoe is missing complex I -

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Absence of Complex I Is Associated with Diminished Respiratory Chain Function in European Mistletoe...

Parasitism is a life history strategy found across all domains of life whereby nutrition is obtained from a host. It is often associated with reductive evolution of the genome, including loss of...

pubmed.ncbi.nlm.nih.gov

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@ruth_hook_

Ruth Hook

1 year

Now we're back in NYC at beautiful Rockefeller University which is a close to Hogwarts as you will ever get on earth where a prodigous stem cell researcher produced a line of ESCs (not iPSCs) called RUES2s & made them widely available & they diff into CMs like a dream (6/n)

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@ruth_hook_

Ruth Hook

2 months

oh yeah one of my startups stopped using genscript for this reason

@KRHornberger

Keith Hornberger

2 months

Some required reading from Derek on the Chinese CRO situation

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@ruth_hook_

Ruth Hook

1 year

also fun fact if you made it this far

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The hypothalamus predates the origin of vertebrates

Some Ciona neurons show transcriptional similarities to the mouse hypothalamus, suggesting that it predates vertebrates.

www.science.org

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@ruth_hook_

Ruth Hook

1 year

love this gradiant of disparity from before and after birth with NPCs being relatively the same to hepatocytes being the most changed

@JShendure

Jay Shendure

1 year

We speculate that these rapid, cell type-restricted changes are poised physiological adapations that are triggered by birth, e.g. gluoconeogenesis in the liver (cut off from mom), thermogenesis by fat (the real world is cold), etc. 14/n

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@ruth_hook_

Ruth Hook

1 month

@kapahi_pankaj is English their first language

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@ruth_hook_

Ruth Hook

1 year

MUSICIANS OF TWITTER what do you tune to

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