Bernardo Sabatini @blsabatini Twitter profile

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Bernardo Sabatini

@blsabatini

5 years

Thank you to all my supporters, well-wishers, collaborators, family, and the amazing people from my lab that did all the hard work over the last 18 years of my lab.

National Academy of Sciences

@theNASciences

5 years

Congratulations Bernardo L. Sabatini @blsabatini of @harvardmed @HHMINEWS , newly elected #NASmember ! #NAS156 #neurobiology

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Bernardo Sabatini

@blsabatini

3 years

1/2 There has been a lot of talk about mentoring recently. At the risk of seeming braggy, this is a writeup about my mentoring style that I put together for the Landis mentoring award (the lab nominated me). Features @VAAlvarez_NIH and @nicxtri !

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Bernardo Sabatini

@blsabatini

5 years

Without attribution - said today by a graduate student in the lab working on a very hard project: "It's like theres a capricious demon pulling the strings of this project to dole out exactly enough results for us to continue but be miserable"

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Bernardo Sabatini

@blsabatini

1 year

For experimental scientists, writing reviews does not equal doing science. Spend the time doing research, making discoveries, and publishing those. I see many CVs of juniorish PIs who write 4:1 reviews to papers. That may build your "brand" but, IMO, is not generally valuable

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Bernardo Sabatini

@blsabatini

4 years

1/n Very proud of this work led by @_JaeeonLee_ It takes a modern bend on analysis of parallel loops through the basal ganglia (building on such classic work by Peter Strick and others) to show separate action linked channels through multiple BG nuclei

Anatomically segregated basal ganglia pathways allow parallel behavioral modulation

Nature Neuroscience - This study demonstrates that basal ganglia functional topography is maintained across and downstream of its output nuclei, and in closed loops. Focal stimulation of distinct...

www.nature.com

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Bernardo Sabatini

@blsabatini

5 years

I wrote this up and posted. In some sense it is trivial, yet it is important. If you are using fluorescent reporters (GCAMP, dLight,....) and relating to behavioral events or neural activity, please read it and comment

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Bernardo Sabatini

@blsabatini

3 years

1/n Now for something fun and really cool Our latest preprint...

Biophysical demonstration of co-packaging of glutamate and GABA in individual synaptic vesicles in...

Many mammalian neurons release multiple neurotransmitters to activate diverse classes of ionotropic receptors on their postsynaptic targets. Entopeduncular nucleus somatostatin (EP Sst+ ) neurons...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

2 years

It's an honor to be working with @cziscience , @ShamKakade6 and many others to build the Kempner Institute at @Harvard . It will push and blur the boundaries of and between computational neuroscience/AI/ML to learn how the brain computes, improve AI/ML and better lives of people.

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Bernardo Sabatini

@blsabatini

7 years

Too much

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Bernardo Sabatini

@blsabatini

5 years

I was recently nominated for the Landis mentoring award from NINDS. Now I learned that many of my past lab members got together and coordinated a big effort to make this happen. I am honored and thrilled by the their support.

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Bernardo Sabatini

@blsabatini

7 years

The pumpkin scope from @SabatiniLab Winner in science category.

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Bernardo Sabatini

@blsabatini

4 years

Our efforts to do metabolomic profiling of synaptic vesicles. Are there neurotransmitters left to be discovered? So far we've proven that glutamatergic vesicles have only glutamate! Led by the formidable Dr. Lynne Chantranupong, formally of @DMSabatini fame

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Bernardo Sabatini

@blsabatini

1 year

Today we in @SabatiniLab proudly celebrate freshly minted Dr. Seul Ah Kim @SeulAhKim2 whose thesis work proved that GABA and glutamate are copackaged in the same vesicles at EP to LHB synapses. Congrats on your great work and defense!

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Bernardo Sabatini

@blsabatini

3 years

1/3 My defense of my brother offended many. I apologize. I did not mean offend nor to minimize the impacts of harassment. All forms of harassment are serious and unacceptable and my sympathy is with those who have suffered.

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Bernardo Sabatini

@blsabatini

4 years

1/3 What we are doing as a lab: 1. Having a conversation about difficult topics 2. Committing $, time, and personal investment to greater recruitment of minority scientists at all levels 3. Plans to reach out to local schools to increase students exposure to science & scientists

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Bernardo Sabatini

@blsabatini

3 years

Snow tree from our window

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Bernardo Sabatini

@blsabatini

3 years

3/3 My brother is the closest person in the world to me. I followed my natural inclination to defend him. I suspect most of you, if put in the same situation, would defend your sibling. This situation is a tragedy for my family – we are grieving. I ask for your understanding.

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Bernardo Sabatini

@blsabatini

5 years

Save the date - it's official Optogenetic Approaches to Understanding Neural Circuits and Behavior July 19-24 2020 Grand Summit Hotel at Sunday River in Newry ME Thrilled to be organizing this with @denisejcai after being vice-chair with the indomitable @kaymtye as chair in 2018

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Bernardo Sabatini

@blsabatini

5 years

I just realized that arrived at Harvard to start my lab 17 years ago today!

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Bernardo Sabatini

@blsabatini

3 years

I'm thrilled that this work, led by @sa_melzer , and in collaboration with @LinTianLab is out! This has been a monumental effort overs years. Sarah is truly an amazing postdoc. Bombesin-like peptide recruits disinhibitory cortical circuits and enh...

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Bernardo Sabatini

@blsabatini

3 years

An amazing thing just happened - I had missed the cut off for @bostonmarathon by ~5 sec. I was really bummed about it. Then out of the blue somehow @SamuelAdamsBeer got me a spot and paid for my entry! Thank you!

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Bernardo Sabatini

@blsabatini

5 years

The latest preprint from our lab for all Dorsal Raphe enthusiasts. Beautiful work led by grad student Kee Wui Huang (handle unknown!). Molecular and anatomical organization of the dorsal raphe nucleus

Molecular and anatomical organization of the dorsal raphe nucleus

The dorsal raphe nucleus (DRN) is an important source of neuromodulators in the brain and has been implicated in a wide variety of behavioral and neurological disorders. Although mostly studied as a...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

3 years

I don’t understand how I ever worked every day until 5. By 2:30 now I’m ready for a nap

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Bernardo Sabatini

@blsabatini

3 years

Big run tomorrow! Going to be rough

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Bernardo Sabatini

@blsabatini

3 years

1/11 In response our ( @SeulAhKim2 et al) preprint showing co-packaging of GABA and glutamate in individual vesicles, there has been a lot of discussion about “Dale’s law”. This series is to clarify misconceptions about “Dale’s law”.

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Bernardo Sabatini

@blsabatini

6 years

I am glad everyone is retweeting but it would be better to download the paper and read it. We need to start a real community in which preprints get discussed and feedback leads to better science.

Bernardo Sabatini

@blsabatini

6 years

Big effort by @ajgrange and company from @SabatiniLab on cortical cholinergic VIP cells is now on biorxiv

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Bernardo Sabatini

@blsabatini

5 years

Here in London with sabalabbers ⁦ @ianOldenburg ⁩ ⁦ @NUro_science ⁩. Perfect setting to learn that I was awarded the NINDS mentoring award. Thanks to these two and all the others that made this happen. I’m honored

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Bernardo Sabatini

@blsabatini

4 years

Does anyone have a good, simple system to allow people to sign up for each piece of equipment or place in the lab? Planing for re-opening. The software has to allow 1 person/equipment time slot and 1 equipment time slow/person. Google calendars is not it, I don't think

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Bernardo Sabatini

@blsabatini

4 years

New England is so pretty in the fall

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Bernardo Sabatini

@blsabatini

6 years

This was my lab in 2003. L to R Veronica Alvarez (PI NIH) Brenda Bloodgood (PI UCSD) me @DennisRidenour (Stowers) Adam Carter (PI NYU) Maria Neimark @neimarkgeffen (PI U Penn) (Maria only rotated in the lab) They've all done great!

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Bernardo Sabatini

@blsabatini

6 months

🧵When and how does the striatum impact learning? Postdoc Kim Reinhold trained mice to reach for food pellets. They didn't know when the food was available so they had to reach randomly. One day, we provided a cue telling them the food was there.

Striatum supports fast learning but not memory recall

Animals learn to carry out motor actions in specific sensory contexts to achieve goals. The striatum has been implicated in producing sensory-motor associations, yet its contribution to memory...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

3 years

Our study, led by ⁦ @MinsukHyun ⁩, is out. It examines the circuitry involved hierarchy dependent changes in micturition (i.e. what makes pee patterns). Lots of cool whole brain datasets in there.

Social isolation uncovers a circuit underlying context-dependent territory-covering micturition |...

The release of urine, or micturition, serves a fundamental physiological function and, in many species, is critical for social communication. In mi...

www.pnas.org

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Bernardo Sabatini

@blsabatini

5 years

Since people still don’t seem to know about tapered fibers, I’m reposting. Check out our several papers. It works and changes how we do opto stim and photometry Depth-resolved fiber photometry with a single tapered optical fiber implant | Nature Methods

Depth-resolved fiber photometry with a single tapered optical fiber implant

Nature Methods - Fiber photometry with tapered fibers allows monitoring of neural activity in larger volumes than with flat-cleaved fibers. In addition, signals from different depths can be...

www.nature.com

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Bernardo Sabatini

@blsabatini

4 years

1/n @ajgrange and team did an enormous amount of work for this study on cortical cholinergic interneurons: Cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target-and brain-region specific manner

Cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific...

Most ChAT-expressing interneurons are a subset VIP+ interneurons that differentially release GABA and acetylcholine onto different post-synaptic targets, while a separate population of non-VIP ChAT+...

elifesciences.org

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Bernardo Sabatini

@blsabatini

4 years

The work led by twitterless Lynne Chantranupong is now out. With our important collaborators in the metabolomics world we screen the contents of synaptic vesicles. Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain

Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain

Extensive mass spectrometry-based profiling of polar metabolites within synaptic vesicles that are rapidly isolated either from cultured mouse neurons or directly from mouse brains reveals their...

elifesciences.org

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Bernardo Sabatini

@blsabatini

4 years

Beautiful and difficult work by the team

HarvardBrainScience

@harvardbrainsci

4 years

HBI News article by @ajgrange on new research from the labs of @blsabatini , Chenghua Gu, and @Harwell_Lab demonstrating that ChAT-expressing interneurons are able to specialize which neurotransmitters they release onto different downstream target neurons.

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Bernardo Sabatini

@blsabatini

5 years

Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus

The PF of the intralaminar thalamus is implicated in human neuropsychiatric disease. Mandelbaum et al. deconstruct the mouse PF using transcription, electrophysiological, and whole-brain anatomical...

www.cell.com

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Bernardo Sabatini

@blsabatini

9 months

Latest from the lab. All about striatum Dopamine/Ach interactions during behavior in mice.

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Bernardo Sabatini

@blsabatini

1 month

Pretty amazing that you can catch solar flares with a 300 mm lens. That is what those are, right? This is an unprocessed image from yesterday’s eclipse taken in northern NH.

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Bernardo Sabatini

@blsabatini

4 years

Finally found a use for it after all these years

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Bernardo Sabatini

@blsabatini

4 years

3/3 I will be more vocal about my Hispanic background and try to make myself more accessible/relatable to minority students. We come from a privileged background but @DMSabatini and I are 1st generation born in USA. Parents are from Argentina and Spanish was our first language.

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Bernardo Sabatini

@blsabatini

5 years

Our latest work is now on available. Cell-type specific asynchronous modulation of PKA by dopamine during reward based learning This is collaborative work led by no-twitter MD/PhD student Suk Joon Lee with @yaochen20 @LinTianLab @SabatiniLab

Cell-type specific asynchronous modulation of PKA by dopamine during reward based learning

Canonical reinforcement learning models postulate that dopamine neurons encode reward prediction error (RPE) and provide a teaching signal to striatal spiny projection neurons (SPNs) in the form of...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

9 months

The new latest from the lab. Why and how does thyroid hormone change exploratory behaviors? Here we show that in mice it acts directly on excitatory cortical projection neurons to re-activate axon guidance programs.

Thyroid hormone rewires cortical circuits to coordinate body-wide metabolism and exploratory drive

Animals adapt to varying environmental conditions by modifying the function of their internal organs, including the brain. To be adaptive, alterations in behavior must be coordinated with the...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

4 years

Check out trailblazer @stevens1lab and 8 fellow scientists in @ScienceMagazine !

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Bernardo Sabatini

@blsabatini

2 years

Another beautiful study from @KhaliqLab . Check out the bonus species in Figure 7. This is probably about the hardest electrophysiology out there1 Fast-synaptic axo-axonal transmission from striatal cholinergic interneurons onto dopamine fibers.

Fast synaptic-like axo-axonal transmission from striatal cholinergic interneurons onto dopaminergic...

Transmission from striatal cholinergic interneurons (CINs) controls dopamine release through nicotinic acetylcholine receptors (nAChRs) on dopaminergic axons. Anatomical studies suggest that cholin...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

4 years

Boston tonight. Reflection in frozen Charles

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Bernardo Sabatini

@blsabatini

4 years

Now a bit of science. This paper from Asha Lahiri in Mark Bevan's lab is excellent. D1R expressing SPNs are made more excitable by dopamine and the effect can last 10 minutes. There is a rapid enhancement of ...

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Bernardo Sabatini

@blsabatini

5 years

Jupiter and moons. iPhone through crappy telescope but still cool

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Bernardo Sabatini

@blsabatini

6 years

Big effort by @ajgrange and company from @SabatiniLab on cortical cholinergic VIP cells is now on biorxiv

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Bernardo Sabatini

@blsabatini

6 months

This is new work from the lab. If you are interested in exactly when and how striatum contributes to learning, take a look. More details to follow. Let by the great Kim Reinhold, now on the job market.

bioRxiv Neuroscience

@biorxiv_neursci

6 months

Striatum supports fast learning but not memory recall #biorxiv_neursci

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Bernardo Sabatini

@blsabatini

4 years

It’s had a good run. But, after about 17 years of near continuous operation, covid has claimed this laser. May not have survived the the prolonged shut down.

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Bernardo Sabatini

@blsabatini

6 years

The product of a nice collaboration with the Greenberg lab - Activity dependent transcriptome across neuronal and non-neuronal cell types in visual cortex Single-cell analysis of experience-dependent transcriptomic states in the mouse visual cortex

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Bernardo Sabatini

@blsabatini

5 years

Monitoring Behaviorally Induced Biochemical Changes Using Fluorescence Lifetime Photometry

Frontiers | Monitoring Behaviorally Induced Biochemical Changes Using Fluorescence Lifetime...

All cells respond to extracellular signals by altering their intracellular biochemical state. In neurons, such signaling regulates many aspects of cell and s...

www.frontiersin.org

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Bernardo Sabatini

@blsabatini

5 years

Our analysis of the molecular and anatomical organization of the DRN is out. Congrats to almost-Dr. Kee Wui Huang and co @eLife

Molecular and anatomical organization of the dorsal raphe nucleus

The dorsal raphe nucleus contains transcriptionally diverse cell classes that include subtypes of serotonergic neurons with distinct molecular and anatomical signatures.

elifesciences.org

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Bernardo Sabatini

@blsabatini

5 years

Oops. Last picture was out of focus. This is reflection of stalactites in underground “lake” which is only about 20 inch deep

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Bernardo Sabatini

@blsabatini

3 years

He's been an ardent supported of under-represented groups in science - through Pew, RWJ and other foundations - as well as through personal efforts for individuals. I love this pic.

JustAnSVD

@DavidSabatini2

3 years

I'd like to take a bit of time to publicize my grandfather, David Domingo Sabatini (seen below kneeling in support of BLM last summer). Today he just turned 90, and for 90 years I think he has been an incredible scientist and mentor to everyone he knows.

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Bernardo Sabatini

@blsabatini

3 years

Arrived at south rim. Super hot but stunning

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Bernardo Sabatini

@blsabatini

1 year

Krishna Shenoy @shenoystanford was a wonderful person and inspirational scientist. I had the privilege of getting to know him over the last ten years and will miss him and his wry commentary.

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Bernardo Sabatini

@blsabatini

6 years

Endogenous Gαq-Coupled Neuromodulator Receptors Activate Protein Kinase A

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Bernardo Sabatini

@blsabatini

4 years

Philly 26.2 in 3:17:15

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Bernardo Sabatini

@blsabatini

2 years

This week we say goodbye to @sa_melzer (Sarah Melzer), one of the most dedicated, talented, and creative postdocs I have encountered. Look out for and join her group, which should be live in Vienna in a few months. Thanks Sarah for having been a part of the lab.

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Bernardo Sabatini

@blsabatini

3 years

Starting the day in Santa Monica/Venice is always a good thing

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Bernardo Sabatini

@blsabatini

2 years

A great opportunity for those interesting in neurobiology and AI. This is the first joint search carried out by Harvard Department of Neurobiology and the new Kempner Institute for the study of natural and artificial intelligence. This is for a "dry lab"

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Bernardo Sabatini

@blsabatini

3 years

Today is tech appreciation day! Thank you to the wonderful crew pictured here who keep @sabatinilab cranking!

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Bernardo Sabatini

@blsabatini

3 years

> 65 days after resubmission and still waiting.... but preprint had been downloaded > 1000 times.

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Bernardo Sabatini

@blsabatini

5 years

⁦ @emory_sabatini ⁩ will be thrilled - he and I are ⁦ @ewarren ⁩ fans. He’s still hoping for a phone call from her due to all his micro donations.

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Bernardo Sabatini

@blsabatini

4 years

1/n We've posted a new study led by @sarahmelzer86 in collaboration with @LinTianLab . Bombesin-like peptide recruits disinhibitory cortical circuits and enhances fear memories

Bombesin-like peptide recruits disinhibitory cortical circuits and enhances fear memories

Disinhibitory neurons throughout the mammalian cortex are powerful enhancers of circuit excitability and plasticity. The differential expression of neuropeptide receptors in disinhibitory, inhibitory...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

4 years

I’ve discovered that obsessive rumination about infuriating reviews of rejected papers makes long planking sessions go by faster.

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Bernardo Sabatini

@blsabatini

3 years

Are you an ambitious budding scientist looking for a 2+ year technician position to hone your skills? Apply below. This is an opportunity to work with a fantastic postdoc in an exciting environment. You'll do and learn a lot.

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Bernardo Sabatini

@blsabatini

2 years

Proud of the extraordinary scope, rigor, and nuance of the analysis done by @Lchantran and team on this project. Please see her tweetprint for details! Local and long-distance inputs dynamically regulate striatal acetylcholine during decision making

Local and long-distance inputs dynamically regulate striatal acetylcholine during decision making

Within the basal ganglia, striatal dopamine (DA) and acetylcholine (Ach) are essential for the selection and reinforcement of motor actions and decision making. In vitro studies have revealed a...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

7 years

We think this is important tech for optogenetics. Move the site of stim on the fly. Or hit large brain regions

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Bernardo Sabatini

@blsabatini

2 years

Beautiful work from student Andrew Landau on calcium signaling in Layer 2/3 pyramids. Some are silent wrt AP-evoked Ca entry! Nice collaboration with @AdamEzraCohen group to voltage image the dendrites and show that APs still arrive. @landoskape will fill in the story.

bioRxiv Neuroscience

@biorxiv_neursci

2 years

Dendritic branch structure compartmentalizes voltage-dependent calcium influx in cortical layer 2/3 pyramidal cells #biorxiv_neursci

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Bernardo Sabatini

@blsabatini

3 years

The five of us welcoming 2021 as best we could with a micro party (just us)

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Bernardo Sabatini

@blsabatini

1 year

Congrats to @shunnnli for passing his PQE today. Great job Shun! @SabatiniLab

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Bernardo Sabatini

@blsabatini

6 years

Anyone doing fiber photometry should read this. Know what you are measuring !

Ferruccio_PhC

@Ferruccio_PhC

6 years

Our last preprint: we try to give a quantitative understanding of the brain volumes from which signal is collected in fiber photometry experiments! @blsabatini @mdv_67 #optogenetics #neuroscience #fiberphotometry #optics #neurophotonics

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Bernardo Sabatini

@blsabatini

3 years

@svoboda314 A method to measure the strength of synapses in vivo continuously over time

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Bernardo Sabatini

@blsabatini

4 years

Big steak for the biggest Sabatini’s birthday

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Bernardo Sabatini

@blsabatini

4 years

1/n Now that we all have some time to rethink our labs... I'd like to know why people have not widely adopted tapered fibers for optogenetic stimulation. The are simple to use, clearly cause less tissue damage, and use power much efficiently. See:

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Bernardo Sabatini

@blsabatini

4 years

Success!

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Bernardo Sabatini

@blsabatini

2 years

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Bernardo Sabatini

@blsabatini

3 years

Congrats to @_JaeeonLee_ who beautifully presented and defended his thesis today. What a poised and creative scientist!

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Bernardo Sabatini

@blsabatini

3 years

I am retweeting now because it links to the explanation of the findings in the just-published Thirteen months later, with lots of rewrites and a few new experiments, the conclusions are the same so please read below.

Bernardo Sabatini

@blsabatini

5 years

Our latest work is now on available. Cell-type specific asynchronous modulation of PKA by dopamine during reward based learning This is collaborative work led by no-twitter MD/PhD student Suk Joon Lee with @yaochen20 @LinTianLab @SabatiniLab

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Bernardo Sabatini

@blsabatini

4 years

Thanks to Hidde for writing this "World view". In 18 years of my lab, only once has reviewer suggested experiment that changed interpretation of our data. All else was fluff and trimming around the edges... often made papers stronger, but never changed conclusion fundamentally.

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Bernardo Sabatini

@blsabatini

5 years

Taller, smarter and better looking. The next generation in action. @DavidSabatini2

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Bernardo Sabatini

@blsabatini

2 years

I find it amazing that scientists on this platform -- supposed rational thinkers who engage in empirically driven logical debate -- publicly refer to me as a genetically doomed piece of shit because I point something factually true.

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Bernardo Sabatini

@blsabatini

5 years

We've posted out transcriptional and anatomical analysis of the lateral habenula (the MHB came along for the ride so it's in there too). Effort led by Mike Wallace in the lab (twitter handle unknown).

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Bernardo Sabatini

@blsabatini

5 years

End of a rainy soaked day in Acadia

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Bernardo Sabatini

@blsabatini

3 years

1/n We've posted a new preprint led by Yao Chen, @yaochen20 , now leading her own lab at Wash U.

The kinase specificity of protein kinase inhibitor peptide (PKI)

G-protein-coupled-receptor (GPCR) signaling is exquisitely controlled to achieve spatial and temporal specificity. The endogenous protein kinase inhibitor peptide (PKI) confines the spatial and...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

3 years

8.5 hours and 13-16 miles later (with @DavidSabatini2 going all the way down and back) we’re back at the rim. Looking worse for wear but intact

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Bernardo Sabatini

@blsabatini

2 years

We have update our manuscript: Efficient and stochastic mouse action switching during probabilistic decision making We now include a reinforcement learning model. The study shows how to draw equivalence between LR, DDM-like, RL, and a "sticky" HMM model

Mice exhibit stochastic and efficient action switching during probabilistic decision making

In probabilistic and nonstationary environments, individuals must use internal and external cues to flexibly make decisions that lead to desirable outcomes. To gain insight into the process by which...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

7 months

Interested in PKA activity? A mouse for that made by @yaochen20 A Cre-dependent reporter mouse for quantitative real-time imaging of Protein Kinase A activity dynamics

A Cre-dependent reporter mouse for quantitative real-time imaging of Protein Kinase A activity...

Intracellular signaling dynamics play a crucial role in cell function. Protein kinase A (PKA) is a key signaling molecule that has diverse functions, from regulating metabolism and brain activity to...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

6 years

Showing the benefits of tapered fibers for photometry Multipoint and large volume fiber photometry with a single tapered optical fiber implant

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Bernardo Sabatini

@blsabatini

4 years

Why doesn't @eLife publish every paper that is reviewed, along with the reviews, and an author reply/resubmission? After review, the vast majority of the work of the reviewers and the editors is done. The community can then weigh in. What's the down side? @mbeisen

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Bernardo Sabatini

@blsabatini

2 years

Fall in Boston

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Bernardo Sabatini

@blsabatini

6 years

This is important and a must read for those interesting in synaptic mechanisms contributing to ASD. It's the best work on potential E/I contributions. It shows that you can't assume that increased excitation relative to inhibition necessarily means that over excitation.

Dan Feldman

@DanFeldmanPhD

6 years

E-I ratio changes in autism promote synaptic homeostasis, not hyperexcitability, in sensory cortex. From Michelle, Phil and Tomer in my lab.

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Bernardo Sabatini

@blsabatini

5 years

On the streets of Boston. Beautiful and in racing green

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Bernardo Sabatini

@blsabatini

4 years

An important analysis of the spike to Ca transient (actually to GECI fluorescence) transform and methods to deal with its nonlinear nature. From @ShaulDr & co

A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology

Calcium imaging with fluorescent protein sensors is widely used to record activity in neuronal populations. The transform between neural activity and calcium-related fluorescence involves nonlinear...

www.biorxiv.org

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Bernardo Sabatini

@blsabatini

3 years

@BWJones I guess although I don't have the crashing feeling. It's more that I can't get done what I enjoy doing, I don't enjoy the little that I do accomplish, I feel like I'm not responsive to everyone's needs, and I seem to be continually asked to do more.

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Bernardo Sabatini

@blsabatini

6 years

There I am along with 3 others from larger Harvard med ecosystem.

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Bernardo Sabatini

@blsabatini

5 years

Why do we still consider (F)ISH necessary to validate scRNAseq data? It is not clear that ISH is a gold standard. We have never failed to reproduce an scRNAseq prediction (from a well QC'd experiment) by FISH. It's time to let that go. It's an enormous waste to time and money.

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