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Nikon Instruments

@NikonInst

5 years

Our @NikonSmallWorld 2019 Photomicrography Contest winners are now live 🔬🏆, click to check them out ->

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@NikonInst

2 years

Cell biologists beware – pre-print research shows how paraformaldehyde fixation can alter the appearance of phase separation when compared to live cells:

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@NikonInst

4 years

Congratulations to all of our incredible #NikonSmallWorld photomicrography contest winners! The first place winner below is by Daniel Castranova and features a dorsal view of a juvenile #zebrafish . Click to check out all of the 2020 winners. #microscopy

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@NikonInst

5 years

We are excited to announce two new #silicone immersion #objective lenses! Click to learn more ->

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@NikonInst

5 years

Nikon is excited to announce #DenoiseAI , a #deeplearning tool for real-time #denoising of resonant-scanning #confocal data -> . Video captured with a Nikon A1R HD25 in resonant mode, before (left) and after (right) denoising.

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Nikon Instruments

@NikonInst

4 months

Our new #MastersofMicroscopy post features Dr. Alexandre Dumoulin, winner of the 2023 #NikonSmallWorld in Motion video #Microscopy competition! His winning video below shows neurons connecting opposite sides of the CNS in a chick embryo. View the post:

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Nikon Instruments

@NikonInst

4 years

Most of us are familiar with magnification and NA, but have you ever wondered about all those other markings on your #microscope #objective lens? If so, then check out our #MicroscopyU article 'Microscope Objective Specifications'.

Microscope Objective Specifications

microscopyu.com

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Nikon Instruments

@NikonInst

1 year

New #SuperResolution #Microscopy method using auto-correlation with two-step deconvolution (SACD) requires only 20 image frames to realize ~2-fold higher resolution with fluctuation-based imaging, great for live cell and high throughput:

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Nikon Instruments

@NikonInst

2 years

Introducing the Nikon Resolution Calculator! This interactive tool makes it easy to calculate #Microscopy specifications such as resolution, depth of field, sampling rate, and more for a variety of imaging modes, including point-scanning #Confocal :

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Nikon Instruments

@NikonInst

5 months

Researchers introduce a 3D-printed wellplate system for #SuperResolution expansion #Microscopy #ExM that allows pre- and post-ExM imaging within the same well, making it easier to visualize distortions:

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Nikon Instruments

@NikonInst

5 years

Want more examples of Nikon’s new #DenoiseAI for resonant scanning #confocal ? Check out these Caco-2 cells stably expressing H2B-GFP, cultured in Matrigel. Image courtesy of Sally Cheung, @PelletierLab , Department of Molecular Genetics, University of Toronto. #deeplearning

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Nikon Instruments

@NikonInst

4 years

is here. This new module expands our NIS-Elements #microscopy #software to include #deeplearning solutions for acquisition and analysis. Advanced #AI capabilities for denoising, segmentation, and more are now at your fingertips:

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Nikon Instruments

@NikonInst

6 years

The wait is over, check out our new N-SIM S high-speed super-resolution structured illumination system! This new model uses a spatial light modulator to image up to 15 frames per second! Movie is 3D stack of keratin in an adherent cell.

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Nikon Instruments

@NikonInst

1 year

Introducing the CFI Plan Apochromat Lambda S 60XC Sil (NA 1.3) – our latest silicone immersion objective lens! Silicone oil is evaporation resistant and better matches the refractive index of thick biological specimens. Learn more: #Microscopy #Imaging

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Nikon Instruments

@NikonInst

5 years

#DenoiseAI of microtubule end-binding protein EB3 in an adherent cell! Click to learn more about DenoiseAI for #denoising of resonant scanning #confocal data ->

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Nikon Instruments

@NikonInst

2 years

Check out this new open-source pipetting robot designed to mount directly on a microscope stage and demonstrated with our Ti2 inverted microscope for #LiveCell #Microscopy experiments:

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Nikon Instruments

@NikonInst

6 months

New extended depth of field (EDF) #Microscopy method uses #DeepLearning for joint design of an EDF-extending binary phase filter and image deconvolution network, extending depth of field by ~15.5X compared to a standard widefield microscope:

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Nikon Instruments

@NikonInst

3 years

Join our #webinar and learn about mapping the axon’s nanoscale architecture with our guest speaker @christlet . Joined by @HenriquesLab as a host, we'll explore the role of #superresolution #microscopy in the discovery of new actin structures in neurons.

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Nikon Instruments

@NikonInst

5 years

Everybody loves the Nikon Ti2 microscope, now meet the stand that started it all - the Nikon Ti-0!!

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Nikon Instruments

@NikonInst

10 months

Researchers apply #DeepLearning -based denoising to #SuperResolution #STED images, allowing for pixel dwell time reduction by more than an order of magnitude. The reduced photobleaching/photodamage allowed for long-term mitochondrial imaging:

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Nikon Instruments

@NikonInst

9 months

Recent research combines #SuperResolution random illumination #Microscopy with #TIRF for multicolor live cell imaging with ~100 nm spatial and ~1-8 Hz temporal resolution:

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Nikon Instruments

@NikonInst

2 years

We are thrilled to announce the upcoming opening of the Nikon Center of Excellence for Neuro-NanoImaging at @univamu on December 9! Stay tuned and learn how this can accelerate neuronal cytoskeleton research: #microscopy @christlet @inp_marseille

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Nikon Instruments

@NikonInst

9 months

Self-Net is a new #DeepLearning method for improving the axial resolution of conventional volumetric fluorescence #Microscopy data, promising a fast and robust approach for attaining isotropic resolution and demonstrated using multiple imaging modalities:

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Nikon Instruments

@NikonInst

1 year

#ImageScanningMicroscopy #ISM has arrived at Nikon! Introducing NSPARC, a SPAD array-based detector for the Nikon AX / AX R #Confocal #Microscopy systems. NSPARC provides #SuperResolution and with superior sensitivity compared to conventional GaAsP PMTs:

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Nikon Instruments

@NikonInst

2 years

Check out this new strategy from @rhodamine110 and colleagues of adding a biotin affinity tag to rhodamine-based HaloTag ligands for straightforward live cell visualization and subsequent purification of HaloTag fusion proteins:

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Nikon Instruments

@NikonInst

3 years

A big thank you to everyone who participated in this year’s @NikonSmallWorld video contest! 1st place video of microfauna in a termite gut by Fabian J. Weston shown below. Click to check out all of this year’s winners. #NikonSmallWorld #microscopy #science

Nikon Small World in Motion 2021 Winners

nikonsmallworld.com

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Nikon Instruments

@NikonInst

7 months

Congrats to @christlet and the Neuro Cyto Lab for having their image of the neuronal cytoskeleton featured on the cover of the Journal of Cell Biology! It was captured using a CSU-W1 SoRa system at our Center of Excellence in Marseille. Click for article:

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Nikon Instruments

@NikonInst

4 years

Looking for an introduction to #lightsheet fluorescence #microscopy #LSFM ? If so, then check out this recent @focalplane_jcs article by @KuglerElisabeth and @ReynaudEmmanuel . They take a look at common LSFM acronyms and geometries users will come across.

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@NikonInst

4 years

Want to know how Nikon’s Perfect Focus System #PFS products work to keep your sample in focus? Then check out our #MicroscopyU tutorial, where you can interactively simulate the effects of vibration and drift. #microscopy

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Nikon Instruments

@NikonInst

3 years

We are delighted to announce the release of the AX and AX R #confocal #microscopy systems - our 10th generation of confocal. The AX/AX R features improved pixel resolution, a large 25mm field of view, and advanced AI-based capabilities. Click to learn more

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Nikon Instruments

@NikonInst

6 months

Pixel reassignment is no longer just for #ImageScanningMicroscopy ! Researchers introduce a simple pixel reassignment-based deblurring algorithm that’s generally applicable to fluorescence #Microscopy data, especially useful for less sparse #SMLM -type data:

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Nikon Instruments

@NikonInst

1 year

A new insight article in @eLife covers recent research into why paraformaldehyde fixation often fails to maintain the appearance of biomolecular condensates formed by liquid-liquid phase separation, highlighting broader concerns about fixation artifacts:

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Nikon Instruments

@NikonInst

3 months

New preprint demonstrates the combination of #SuperResolution fluorescence lifetime single molecule localization #Microscopy #SMLM with #ImageScanningMicroscopy for doubling the localization precision (XY) compared to SMLM alone:

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Nikon Instruments

@NikonInst

5 years

We look forward to seeing everyone at #SfN2019 ! Come find us at #Booth1201 to check out our latest #microscope solutions for #neuroscience . @SfNtweets We will also be revealing @NikonSmallWorld winners on Monday, October 21st so be sure to drop by! 🔬🥇

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Nikon Instruments

@NikonInst

2 years

Check out some of this year's #NikonSmallWorldInMotion video #Microscopy competition winners - with a special congratulations to @ezattara on their 1st place finish! Click to see the full 2022 gallery: #NikonSmallWorld #Imaging #SciArt #SciComm

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Nikon Instruments

@NikonInst

6 months

Check out the latest in #DeepLearning -based image denoising for #Fluorescence #Microscopy ! DeepSeMi is a self-supervised method demonstrated for extended multicolor #LiveCell #Imaging in Dictyostelium cells. Learn more:

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Nikon Instruments

@NikonInst

6 years

Thanks again to everyone who participated in our 8th Annual Small World in Motion #SWIM digital video competition! Video below featuring some of this year's amazing entries. @NikonSmallWorld

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@NikonInst

4 years

Researchers combine expansion #microscopy #ExM with #superresolution single molecule localization microscopy for even higher resolution imaging of microtubules, successfully resolving their 9-fold symmetry.

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Nikon Instruments

@NikonInst

2 months

Researchers demonstrate the applicability of the StayGold fluorescent protein and various Janelia Fluor dyes for multi-color in-resin correlative light and electron #Microscopy #CLEM :

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Nikon Instruments

@NikonInst

6 years

Whoa, here’s a #smartphone attachment for doing #TIRF microscopy. @OSAPublishing

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Nikon Instruments

@NikonInst

2 years

Check out this application of #SuperResolution #LiveCell stimulated emission depletion #STED #Microscopy of the mitochondrial inner membrane using a novel fluorescent dye:

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Nikon Instruments

@NikonInst

2 years

New @biorxivpreprint research shows how the DeepSTORM neural network can be trained to predict #superresolution reconstructions from high emitter density DNA-PAINT data, demonstrated for imaging of neuronal tissue: #SMLM #microscopy #imaging

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Nikon Instruments

@NikonInst

3 months

New preprint provides a step-by-step protocol for #SuperResolution ultrastructure expansion #Microscopy #ExM of kidney tissue, demonstrating its use for human and mouse samples:

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Nikon Instruments

@NikonInst

2 years

Beautiful #confocal #microscopy image of #zebrafish scales and vessels by @dancastranova on this month’s cover of @Dev_journal ! Be sure to check out the article it's from, which demonstrates a new method for long-term imaging of adult zebrafish:

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Nikon Instruments

@NikonInst

9 months

Announcing new near-infrared #NIR imaging capabilities for our AX / AX R #Confocal #Microscopy system! 730 nm and 785 nm laser options will soon be available, along with optimized detector options with high quantum efficiency in the NIR. Learn more:

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Nikon Instruments

@NikonInst

4 years

In one of two new exciting #neuroscience #microscopy publications from the lab of Dr. Na Ji at @UCBerkeley , two-photon Bessel beam imaging is demonstrated in conjunction with a mesoscope for rapid volumetric imaging over a 5-mm field of view ->

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Nikon Instruments

@NikonInst

5 months

New Fourier #LightFieldMicroscopy method from @pengfei16 and colleagues leverages a low-cost diffractive optical element and #DeepLearning -based reconstruction to realize single shot volumetric imaging with resolution comparable to #Confocal #Microscopy :

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Nikon Instruments

@NikonInst

8 months

Announcing the winners of the 13th annual #NikonSmallWorld in Motion video #Microscopy competition! Congrats to Dr. Alexandre Dumoulin for his 1st place video of developing neurons connecting to the opposite side of the CNS in a chick embryo. Learn more:

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Nikon Instruments

@NikonInst

4 years

It’s official! Our @NikonSmallWorld judges have been selected: Dr. Dylan Burnette @MAG2ART , Dr. Christophe Leterrier @christlet , Samantha Clark @samanthabclark , Sean Greene @seangreene89 , and Ariel Waldman @arielwaldman will judge this year's #microscopy photo and video contests.

Nikon Instruments Announces Judging Panel For The 46th Nikon Small...

nikonsmallworld.com

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Nikon Instruments

@NikonInst

3 years

REMINDER - our #webinar about mapping the axon’s nanoscale architecture with guest speaker @christlet is this Thursday! With @HenriquesLab as a host, we'll explore the role of #superresolution #microscopy in the discovery of new actin structures in neurons

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@NikonInst

3 years

Researchers from @embl combine 3-photon excitation and sensor-less adaptive optics for high resolution intravital imaging more than 1.4 mm deep in a mouse brain. Click to learn more:

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Nikon Instruments

@NikonInst

19 days

Refractive index mismatch results in axial (Z) distortion – changing the apparent position of the focal plane. Researchers have now derived a theory allowing for simple calculation of a depth-dependent rescaling factor for axial distance in #Microscopy :

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Nikon Instruments

@NikonInst

7 months

Thank you to Dr. Christophe Leterrier @christlet for providing his thoughts on the ease of imaging with the NSPARC #SuperResolution #ImageScanningMicroscopy detector for our AX R #Confocal #Microscopy system! Click to learn more about the system:

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Nikon Instruments

@NikonInst

2 years

Researchers combine label-free 3D holotomography with #deeplearning to predict fluorescence staining patterns for various subcellular structures based on refractive index:

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@NikonInst

2 months

Researchers combine #SuperResolution ultrastructure expansion #Microscopy #ExM with NHS-ester pan-labeling to illuminate the subcellular organization of the pathogenic fungus Candida albicans. Click to view the preprint article:

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Nikon Instruments

@NikonInst

4 years

Check out these winning entries to the 2020 Art of Neuroscience competition, held by the Netherlands Institute for Neuroscience @NIN_knaw ! This competition is in its 10th year and welcomes all sorts of media, including #microscopy images. Learn more:

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Nikon Instruments

@NikonInst

2 months

New review article on #Multiphoton #Microscopy using two-photon excitation #2PE ! This review covers different imaging modes using 2PE, emphasizing applications in #Neuroscience research:

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Nikon Instruments

@NikonInst

2 months

Researchers introduce a new tool for #SuperResolution single molecule localization #Microscopy #SMLM across a large field-of-view using a spatially variant PSF:

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Nikon Instruments

@NikonInst

4 years

Are you into tissue clearing for #microscopy ? The Nikon CFI90 20XC Glyc #objective provides an ultralong working distance of 8.2mm, NA = 1.0, and superior transmittance and aberration correction to 1300nm. Click to learn more. #neuroscience #multiphoton

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@NikonInst

5 years

Check out this real-time #polychromatic #polarization #microscope movie of baby #squid ! Movie was captured by Dr. Elizabeth Lee ( @UChicago ) and @mshribak ( @MBLScience ).

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@NikonInst

1 year

Recent preprint research introduces a novel dye for visualizing the mitochondrial inner membrane using #SuperResolution #STED #Microscopy , applied towards live imaging of the interaction of the inner membrane and mtDNA:

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Nikon Instruments

@NikonInst

2 years

Check out this interesting new application of #deeplearning in cellular imaging – learning the rules governing cell competition directly from in vitro time-lapse #microscopy data to predict cell fate, with potential applications in drug discovery research:

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Nikon Instruments

@NikonInst

4 years

Heard of #superresolution single molecule light field #microscopy ? Light field imaging allows single molecule observation from several angles, with 3D position fitting based on parallax. 20 nm localization precision is demonstrated over a 6um range.

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@NikonInst

6 months

Our own Dr. Sabrina Delattre has an article featured in @MicroscopyToday on the #NSPARC #SuperResolution #ImageScanningMicroscopy detector for our AX / AX R #Confocal #Microscopy system. Image of live mitochondria acquired with NSPARC. Click for article:

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Nikon Instruments

@NikonInst

3 months

Preprint research describes Phoenix Fluor 555, a super-photostable dye for #Fluorescence #Microscopy . The authors demonstrate that its photostability in live cells can be an order-of-magnitude greater than leading dyes in the same spectral class:

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Nikon Instruments

@NikonInst

2 years

Announcing the new AX R MP #multiphoton #microscopy system! The AX R MP features a 22 mm field of view, 2k x 2k resonant scanner, dedicated upright microscope stand options for maximizing space under the objective, and a new tilting objective nosepiece:

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Nikon Instruments

@NikonInst

9 months

Interested in #SuperResolution expansion #Microscopy #ExM , but not sure where to start? If so, then check out this @focalplane_jcs primer focusing on the latest advances in ExM:

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Nikon Instruments

@NikonInst

2 years

Attention #FOM2022 attendees - please join our talk tomorrow with Prof. Vicidomini of the Italian Institute of Technology! He will discuss #ImageScanningMicroscopy and #SPAD array detectors. #Imaging #Microscopy #Superresolution

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@NikonInst

6 years

Cool new method for immobilizing organisms with #PEG hydrogel, including awesome #lightsheet microscopy data! @MBLScience

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@NikonInst

3 months

Happy International Day of Women and Girls in Science! At Nikon Instruments, we're honored to stand alongside these brilliant minds, providing the tools and technologies that enable groundbreaking research and breakthroughs 🔬💛🖤 #WomenInScience #WomenInSTEM #GirlsInScience

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@NikonInst

5 years

We know you can’t get enough #DenoiseAI ! Learn how DenoiseAI applies #deeplearning to #denoising resonant scanning #confocal data -> Volume projection time-lapse of the ER (green) and mitochondria (magenta), acquired during QFM 2019 course at @MDIBL .

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Nikon Instruments

@NikonInst

4 years

Great to see the @NikonSmallWorld photomicrography competition winning image by @dancastranova featured on the cover of @naturemethods ! Click to check out their editorial concering this year’s competition:

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@NikonInst

5 years

Here's a step-by-step guide to #Kohler illumination and phase contrast alignment on the Nikon #Ti2 inverted #microscope .

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@NikonInst

11 months

Researchers introduce SNAP-tag mimics of fluorescent proteins (SmFPs) – combining a SNAP-tag variant with a palette of fluorogenic labels that are non-fluorescent in solution and strongly fluorescent when bound due to conformational locking. Learn more:

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@NikonInst

6 years

Researchers combine new #PAINT probes with #STORM and other techniques for high performance multicolor localization imaging of bacteria. @rhodamine110

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@NikonInst

2 months

There are also many contemporary women in #Microscopy who are making history. Prof. Xiaowei Zhuang is a renowned pioneer of both #SuperResolution microscopy and #SpatialTranscriptomics with her invention of STORM and MERFISH, respectively:

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@NikonInst

2 years

We're excited to announce the Digital Sight 10 #microscope #camera , which can be easily switched between color and monochrome detection modes. The Digital Sight 10 also features 6000 x 3984 pixels and supports large 25 mm field of view imaging. Learn more:

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@NikonInst

4 years

Interesting article about the work of @manorlaboratory at the @salkinstitute applying #deeplearning towards the #decrappification of #microscopy data also nicely highlights the importance of collaboration ->

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Nikon Instruments

@NikonInst

2 years

Check out this combination of #SuperResolution single molecule localization #Microscopy #SMLM and expansion microscopy #ExM for imaging the centriole distal appendage with sub-5 nm localization precision:

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Nikon Instruments

@NikonInst

2 years

Researchers combine #superresolution DNA-PAINT with fluorescence lifetime imaging #microscopy #FLIM to realize lifetime-based multiplexing using a single excitation source in the context of both laser scanning #confocal and widefield instruments:

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@NikonInst

7 months

New sensorless adaptive optics method for #SuperResolution single molecule localization #Microscopy #SMLM uses #DeepLearning to infer wavefront distortions from emitters in the raw data of instead of iterative mirror changes and image quality evaluation:

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@NikonInst

3 months

Refractive index mismatch (RIM) between the sample and immersion medium can greatly limit 3D #Microscopy . A new preprint introduces a method for calculating a depth-dependent re-scaling factor to compensate for axial deformation due to RIM:

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@NikonInst

5 months

Researchers explore photoblueing of #FluorescentProteins in the miRFP family, which convert into a far-red emitting species after exposure to near-infrared light, allowing their use as optical highlighters for #LiveCell #Microscopy of tagged organelles:

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@NikonInst

3 years

Recent article from @cziscience highlights the work of their imaging grantees – including how @MillisBryan is advancing #microscopy education, how @JFitzpatrickWU is developing multi-scale imaging tools, and more! #ImagingtheFuture

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@NikonInst

2 years

Announcing the winners of the 2022 #NikonSmallWorld competition! 1st place this year went to this image of a Madagascar giant day gecko hand, captured by Grigorii Timin of the University of Geneva. Check out the gallery of winners: #Microscopy #Imaging

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@NikonInst

5 years

Can you recognize artifacts in your #SIM data? Figure 1 from below provides examples of important artifacts such as hatching, honeycomb, and ghosting! #superresolution @MicronOxford

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@NikonInst

8 months

New hybrid deterministic and #DeepLearning -based denoising method for fluorescence #Microscopy is applicable to different techniques and using CMOS, CCD, and PMT detectors. This method accounts for read, shot, dark, and fixed pattern noise. Learn more:

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@NikonInst

4 months

New review article on #SuperResolution #Fluorescence #Microscopy methods for #CellBiology covers key factors to consider, including detailed discussion of sample preparation concerns. Check it out:

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Nikon Instruments

@NikonInst

4 years

With so many great entries in our annual @NikonSmallWorld photomicrography competition, you may have missed some of the other great images among this year’s winners! Here are a few of our 2020 Honorable Mentions and Images of Distinction. Click for more:

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Nikon Instruments

@NikonInst

1 year

What do the researchers behind #SuperResolution #ImageScanningMicroscopy #ISM have to say about the Nikon NSPARC? Check out this interview with Drs. Alberto Diaspro @AD1959 , Giuseppe Vicidomini @VicidominiLab , and Paolo Bianchini @2Pbianchini of @IITalk :

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@NikonInst

4 years

The Nikon N-SIM S provides #superresolution #microscopy imaging at the speed of life, with acquisition speeds up to 15 fps. This is fast enough to capture cellular details such as the ER dynamics in the video below. Click to learn more:

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@NikonInst

10 months

Our AX R MP #Multiphoton #Microscopy system can now be combined with the NSPARC #SuperResolution #ImageScanningMicroscopy detector! Acquire super-resolution #ISM images with improved signal-to-noise deep in challenging specimens. Learn more:

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1 month

Review article covers practical considerations for applying different #SuperResolution #Microscopy methods in #CellBiology research, with a focus on sample preparation and probe selection:

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@NikonInst

2 months

New #Optogenetic system allows for the controlled release of single molecules in live cells and is based on a UV-cleavable fluorescent protein. Molecules are released at levels compatible with single molecule imaging. Learn more: #Microscopy #Imaging

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@NikonInst

1 year

Check out this new combination of single objective #LightSheet #Microscopy with multifocal detection using a multifocal grating for performing #SuperResolution single molecule localization microscopy #SMLM :

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@NikonInst

3 years

Recent @biorxivpreprint shows the utility of combining tdTomato or mScarlet red #fluorescentproteins with EGFP and a single laser source in the range of about 1250 – 1350 nm for simultaneous 3-photon excitation of both probes: #multiphoton #microscopy

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3 years

The first Nikon BioImaging Lab in Europe is now open! You can visit us at the prestigious Leiden Bio Science Park and find out how to accelerate you research with assisted access to cutting-edge microscopy systems.

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Nikon Instruments

@NikonInst

3 years

The #NikonSmallWorld 2021 image competition results have been announced! A special congratulations to @jasonmileskirk on his 1st place image of oak leaf trichomes and stomata. Click to check out all the winners. #microscopy @NikonSmallWorld

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@NikonInst

6 years

Researchers at #calicolabs revive remote refocus for crazy fast #zstack acquisitions. Video of C. elegans at 20 volumes/s taken from online pre-print at

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Nikon Instruments

@NikonInst

2 months

Check out this #SuperResolution #Microscopy method using a diffusing filter to scatter high spatial frequency light into the entrance pupil, recovering the image with an iterative wavefront correction process:

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