Steve Massey @stevenemassey Twitter profile

Pinned Tweet

Steve Massey

7 months

The Zhang group of Fudan University have identified and validated two A-B intermediate SARS2 genomes from the early pandemic This provides a key to understanding the origin of COVID19 🧵

14

105

278

Last Seen Profiles

@Ahdk_0304

@sotwecom

@ManciniEdgardo

@bokeplokalmalam

@NorthernParrots

@MINDTRAVEL_info

@Cabams_Is_Lost

@albayrak_berkan

@RichardBlackb18

@mycutiesamocat

@MonikaSimp11441

@TAUGAMMAPHI_68

@lndn99

@NextGen713

@qA0l0SpYSueKSg9

@MetAliensVRclub

@lucasnobreaj

@grange_first

@toxxxbaEMI

@TopcareLandsKE

@Prince_Crooms

@cwaytablewater

@bokeplokalmalam

@riley_stig24393

@JimShoe68100547

@IntCupid

@SCKY_WBB

@buzzinmonkey

@riaria__3

@banmin_god

@AstralAkua

@YwsfAhmd28205

@lingfongchow

@m3043382139731

@YesEquality2015

@Putraa66909812

Steve Massey

@stevenemassey

17 days

A leak of polio from a research facility is indicated by a poliovirus genome sequence generated from a sample collected in 2014 in China by the Wuhan Institute of Virology The sequence is 99 % identical to that of a polio strain from 1954 🧵

Opening a 60-year time capsule: sequences of historical poliovirus cold variants shed a new light...

Abstract. Polioviruses (PVs) are positive strand RNA viruses responsible for poliomyelitis. Many PVs have been isolated and phenotypically characterized in

academic.oup.com

49

472

1K

Steve Massey

@stevenemassey

2 years

Our new paper describing the discovery of a novel SARS2 related coronavirus is out! This coronavirus, which we term 'GX_ZC45r', bears signatures of genetic manipulation

Forensic Analysis of Novel SARS2r-CoV Identified in Game Animal Datasets in China Shows Evolution...

Pangolins are the only animals other than bats proposed to have been infected with SARS-CoV-2 related coronaviruses (SARS2r-CoVs) prior to the COVID-19 pandemic. Here, we examine the novel SARS2r-CoV...

www.mdpi.com

29

353

768

Steve Massey

@stevenemassey

2 years

We have uncovered evidence of undocumented GOF work in Wuhan 🧵 We have identified an infectious clone (IC) of a novel MERS-related coronavirus in rice sequencing datasets from Huazhong Agricultural University (HZAU), Wuhan

Discovery of a novel merbecovirus DNA clone contaminating agricultural rice sequencing datasets...

HKU4-related coronaviruses are a group of betacoronaviruses belonging to the same merbecovirus subgenus as Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV), which causes severe respiratory...

www.biorxiv.org

36

281

665

Steve Massey

@stevenemassey

2 years

If they suspend Daoyu, who has done so much to investigate the Lab Leak hypothesis, they'll be coming for YOU next - if you dare to express an original thought....

25

132

400

Steve Massey

@stevenemassey

1 year

When were the only two known pre-pandemic SARS2-related live isolates (attributed to pangolins) isolated ? And when were they sequenced ? And why does one of them have SARS2 reads in its sequence file ? 🧵

19

84

223

Steve Massey

@stevenemassey

2 years

@humblesci Daoyu @ydeigin @quay_dr and myself have published a study that shows the exclusion of 20 A/B intermediate SARS2 genomes from the analysis of Pekar et al. is unwarranted 🧵

Unwarranted exclusion of intermediate lineage A/B SARS-CoV-2 genomes is inconsistent with the two...

Pekar et al. (2022) propose that SARS-CoV-2 was a zoonotic spillover that first infected humans in the Huanan Seafood Market in Wuhan, China. The basis for their analysis is the hypothesis that there...

zenodo.org

33

81

221

Steve Massey

@stevenemassey

3 years

Evidence for ‘live’ RaTG13 at WIV Conclusion Here, I conclude this series of threads demonstrating RaTG13 was generated from a bat transcriptome and not a fecal swab. This implies it was live cultured, contradicting public statements made by the scientists who worked with RaTG13

23

85

219

Steve Massey

@stevenemassey

2 years

Apparently, pointing out the fundamentals of molecular evolution (that the SARS2 FCS didn't arise from nonsynonymous point mutations, but rather all available data indicates it is an insertion) gets you blocked ! Strange way to concede the point

36

43

217

Steve Massey

@stevenemassey

2 months

41 logicians declare that forwarding a hypothesis 'harms' science In the title they puzzlingly state the lab leak hypothesis is 'without evidence' Comically, the Abstract immediately contradicts this by saying there is no 'strong evidence' Which one is it ? 🤡

26

49

212

Steve Massey

@stevenemassey

11 months

Nice of him to make this decision on behalf of everyone on the planet (what possible danger could come from mixing grandiosity and GOF?)

Ab~ishek

@abhishek_s_1

11 months

@ScienceMagazine @ScienceTM

1

15

59

12

54

195

Steve Massey

@stevenemassey

2 months

Why are virologists freely allowed to anonymize lethal synthetic viruses, but developers are put in jail for writing code that anonymize bitcoin transactions ? Genetically enhanced infectious clones present a much higher risk than anonymous monetary transactions 🧵

4

67

189

Steve Massey

@stevenemassey

1 month

Dr Rasmussen belongs to that peculiar tribe of experimental biologists who claim deep insight into bioinformatics analysis, yet has never done a Blast search Our data is robust to replication

9

33

190

Steve Massey

@stevenemassey

8 months

What was the restriction enzyme BsmBI, itemized in the DEFUSE budget, to be used for? 🧵 3 possibilities: i) To make an spike ectodomain expression vector ii) To insert recombinant S genes into VRP vectors iii) To make infectious clones (ICs)

8

74

178

Steve Massey

@stevenemassey

2 years

So, Flo Debarre et al’s raccoon dog analysis that has caused such a media frenzy has been released and what does it show ? Not much 🧵

15

48

174

Steve Massey

@stevenemassey

1 year

In their survey of the Huanan Seafood Market, Liu et al conclude that there is no evidence for a zoonotic transmission of SARS2 at the market Out of curiosity, I did a deep dive into sample Q61 (Env_0576), which they report as having a high proportion of raccoon dog reads 🧵

9

57

173

Steve Massey

@stevenemassey

7 months

Was Baric aware of the work on the human α-ENaC furin cleavage site (FCS) at the University of North Carolina (UNC) ? 🧵 In a striking coincidence, the human α-ENaC FCS is exactly the same as that of SARS2, as first noted by Anand et al in 2020

SARS-CoV-2 strategically mimics proteolytic activation of human ENaC

SARS-CoV-2 has evolved to cleverly mimic the FURIN-cleavage site in human ENaC-α, unlike any prior coronavirus strain, shedding new light on the Acute Respiratory Distress Syndrome (ARDS) in COVID-19...

elifesciences.org

10

57

169

Steve Massey

@stevenemassey

2 years

14/ In summary, our results indicate that a novel, unreported SARS2-related coronavirus has recently been subject to genetic manipulation in China. These activities are of great potential concern in terms of Biosafety, and the surveillance of Gain of Function research

5

63

156

Steve Massey

@stevenemassey

5 months

Some further insights into the SARS2 spike sequences found in Pseudomonas aeruginosa datasets, recorded as being sampled in 2019 🧵

12

70

163

Steve Massey

@stevenemassey

17 days

2/ Chesnais et al sequenced 3 poliovirus genomes recovered from 60 year old historic samples generated by Albert Sabin at the Pasteur Institute, Paris Sabin is the father of the oral polio vaccine, created from attenuated polio strains

4

30

163

Steve Massey

@stevenemassey

1 year

Despite emerging in Wuhan, the city with the most expertise in tracing coronavirus outbreaks in the world, there is still no sign of an intermediate host for SARS2 The likely intermediate host for SARS1 (palm civet) was identified just months after the initial outbreak

13

28

156

Steve Massey

@stevenemassey

1 year

I've identified numerous vertebrate RNA viruses in the Q61 raccoon dog sample from Liu et al's survey of the Huanan Seafood Market Their % genome coverages are substantially higher than that of SARS2, and many are associated with animals identified previously in the dataset 🧵

14

50

153

Steve Massey

@stevenemassey

6 months

At last Baric next please

Select Subcommittee on the Coronavirus Pandemic

@COVIDSelect

6 months

🚨BREAKING🚨 EcoHealth Alliance President Dr. Peter Daszak will testify at a public hearing on May 1, 2024. Dr. Daszak must answer questions about COVID-19 origins, dangerous gain-of-function research, and his relationship with the Wuhan Institute of Virology in China.

471

2K

4K

11

38

153

Steve Massey

@stevenemassey

1 year

What is the true trace level of SARS2 in the raccoon dog sample Q61 from the Huanan Seafood Market ? Here, in a refined analysis, I show that human common cold coronaviruses are present in quantities greater than SARS2 in Q61 🧵

7

51

148

Steve Massey

@stevenemassey

5 months

This being the case, why did Daszak try so hard to suppress the Lab Leak theory (rather than call for a WIV audit) ?

Select Subcommittee on the Coronavirus Pandemic

@COVIDSelect

5 months

🚨BREAKING🚨 EcoHealth Alliance President Dr. Peter Daszak ADMITS "of course" the Wuhan Institute of Virology could have hidden coronaviruses. Dr. Daszak also does "not know" if the CCP deleted any coronavirus sequences or samples.

102

615

1K

13

33

143

Steve Massey

@stevenemassey

2 years

Another top public health official, this time Dr Deborah Birx, thinks SARS2 escaped from a Wuhan lab Dr Birx is an infectious diseases expert and former presidential COVID19 advisor

Trump aide claims Covid 'came out of the box ready to infect'

Infectious diseases expert Dr Deborah Birx told The Mail on Sunday that coronavirus 'came out of the box ready to infect' when it emerged in the Chinese city of Wuhan in December 2020.

www.dailymail.co.uk

14

38

134

Steve Massey

@stevenemassey

5 months

Differential gene expression analysis of the controversial RaTG13 dataset reveals strong similarity to the RaTG15 dataset, also described as generated from a Rhinolophus affinis 'rectal swab' from the Mojiang Mine This indicates they have a common, undefined source 🧵

13

48

142

Steve Massey

@stevenemassey

1 year

Apparently it's OK with some researchers to have a once in a generation f*ck up that kills 20 million people

Tom Kelly ケリー・トム

@tomkXY

1 year

@jbkinney First, you’ve not shown an evidence that COVID wasn’t natural. Bold assumption. Even if it was, it’s a once in a generation fuck-up while thousands of virology and GMO labs have operated safely for years. See why the people operating them are pissed off? You need to clam down.

170

0

10

13

25

140

Steve Massey

@stevenemassey

3 years

Dear oh dear, I thought I was being constructive by pointing out @stuartjdneil 's numerous factual errors Puzzling he would take offence, as he likes to point out the errors of others with gusto (and nothing wrong with that)

9

14

132

Steve Massey

@stevenemassey

17 days

3/ Poliovirus is a single stranded positive sense RNA virus, that only naturally infects humans (no other natural hosts are known) There are 3 poliovirus serotypes: PV-1, PV-2 and PV-3

3

29

138

Steve Massey

@stevenemassey

9 months

Bombshell ! From an early draft of DEFUSE: "The BSL-2 nature of work on SARSr-CoVs makes our system highly cost-effective relative to other bat-virus systems"

Emily Kopp

@emilyakopp

9 months

You may have heard that a research proposal called DEFUSE lays out a blueprint for the creation of COVID. You may also know that other scientists brush off DEFUSE because the risky virology was to take place in North Carolina. Today new docs obtained by me indicate American

154

1K

3K

5

36

135

Steve Massey

@stevenemassey

2 years

Low levels of bacteria in the 'rectal swabs' used to generate 7896 clade sarbecovirus genomes 🧵 On the trail of the true identity of RaTG13 I decided to look at the 7876 clade NGS datasets (which include RaTG15), as these were also generated from the Mojiang mine....

7

32

124

Steve Massey

@stevenemassey

1 year

18/ In conclusion, there is nothing to link the SARS2 sequences to raccoon dogs in particular, out of all the animals detected, and its low level in the sample is consistent with other human coronaviruses also present at the Huanan Seafood Market

14

32

126

Steve Massey

@stevenemassey

2 years

"Fauci received 23 <undisclosed> royalty payments" Let me guess what for, and who from 🤔

zerohedge

@zerohedge

2 years

Democrats Silent As Republicans Rip Into Secret Royalty Checks To Fauci, Hundreds Of NIH Scientists

34

325

698

4

43

121

Steve Massey

@stevenemassey

9 months

Daszak claims to have discovered a bat CoV from Thailand with 'almost' as much potential to infect humans as SARS2 No mention of a FCS Disturbingly brags about doing the labwork at BSL2

Controversial zoologist is gathering pathogens with pandemic potential

British zoologist Dr Peter Daszak, a central and controversial figure in the debate about Covid's origins, has revealed he's found a close relative of the virus living in bats found in Thai caves.

www.dailymail.co.uk

19

46

118

Steve Massey

@stevenemassey

10 months

This statement about DEFUSE by Eddie Holmes is misleading. DEFUSE describes the identification of potential FCSs in the spike gene of SARSr-CoVs. FCSs are typically found at the S1/S2 boundary...... 🧵

8

36

120

Steve Massey

@stevenemassey

3 years

@MJnanostretch Welcome to the Big Science - Big Media - Big Government - Big Pharma continuum a Gilded Age we are living in !

1

7

111

Steve Massey

@stevenemassey

2 years

I suppose it's dangerous to the conspirators.....

Alice Smith

@TheAliceSmith

2 years

369

4K

18K

5

35

108

Steve Massey

@stevenemassey

9 months

If not a smoking gun, at least a smouldering one

Documents show plan to put spike proteins on SARS-CoV viruses in 2018

A newly-uncovered trove of documents detailing plans to create a Covid-like virus in China months before the pandemic make the 'lab leak almost certain', experts say.

www.dailymail.co.uk

1

32

115

Steve Massey

@stevenemassey

7 days

Baric : "we were fundamentally interested in why didn't sarbecoviruses have a furin cleavage site" It's because there is no (natural) selection for an FCS They may also be maladaptive in a natural environment h/t @Rebecca21951651

5

38

116

Steve Massey

@stevenemassey

1 month

Our discovery of apparent GOF research on MERS-related coronaviruses in pre-pandemic Wuhan has now been published h/t to the brilliant @humblesci @Daoyu15 @ydeigin @BiophysicsFL @quay_dr

Steve Massey

@stevenemassey

2 years

We have uncovered evidence of undocumented GOF work in Wuhan 🧵 We have identified an infectious clone (IC) of a novel MERS-related coronavirus in rice sequencing datasets from Huazhong Agricultural University (HZAU), Wuhan

36

281

665

5

48

114

Steve Massey

@stevenemassey

2 years

Our new paper on intermediate lineage A-B SARS2 genomes is out ! Within, we criticize the exclusion of 20 potential A-B intermediate genomes from Pekar et al (2022), finding that the majority were improperly excluded 🧵

Unwarranted Exclusion of Intermediate Lineage A-B SARS-CoV-2 Genomes Is Inconsistent with the...

Pekar et al. (2022) propose that SARS-CoV-2 was a zoonotic spillover that first infected humans in the Huanan Seafood Market in Wuhan, China. They propose that there were two separate spillovers of...

www.mdpi.com

8

44

108

Steve Massey

@stevenemassey

2 years

We have discovered 6 more A/B intermediate SARS2 genomes (with C/C genotype) from Wuhan 🧵 This is in addition to the 7 new intermediates (also C/C) we previously identified from Sichuan 👇

Steve Massey

@stevenemassey

2 years

We have discovered 5 more A/B intermediate genomes from Sichuan (with a C/C genotype), in addition to 2 we found previously 🧵 These were not considered by Pekar et al, despite 2 of the 5 conforming to their inclusion criteria 👇

11

36

99

6

48

106

Steve Massey

@stevenemassey

2 years

@WashburneAlex I had one manuscript rejected on the basis that it might cause a terrorist attack (no kidding) I had no idea network analysis was so controversial, shows what fragile times we are living in 🙃

Social network analysis of the biblical Moses - Applied Network Science

Here, social network analysis approaches are used to characterize the figure of the biblical Moses, and his relationship with characters from the books of the Pentateuch; Genesis, Exodus, Leviticus,...

appliednetsci.springeropen.com

1

10

105

Steve Massey

@stevenemassey

7 months

17/ 3) This indicates a date of emergence of no later than ~ Oct 2019, as per Kumar et al This is based on the number of mutations needed (3) to get to proCoV2 from the lineage B reference sequence (Hu-1)

An Evolutionary Portrait of the Progenitor SARS-CoV-2 and Its Dominant Offshoots in COVID-19...

Abstract. Global sequencing of genomes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has continued to reveal new genetic variants that ar

academic.oup.com

4

29

109

Steve Massey

@stevenemassey

17 days

6/ Surprisingly, the Glenn genome showed 95 % identity to WIV14, generated from a sample collected in Dec 2014 by the Wuhan Institute of Virology (WIV) WIV14 (accession KY703697) was isolated in Vero cells. from a child in Anhui, China, described 👇

1

16

108

Steve Massey

@stevenemassey

2 years

NIH deleted two Pangolin metagenomic datasets on March 16 2020 at the request of Kangpeng Xiao of South China Agricultural University Inadvertantly revealed by a FOIA request filed by Empower Oversight

NIH accidentally unmasks official who hid Chinese-submitted coronavirus data in March 2020

Agency asks court to seal records related to FOIA lawsuit that name NIH "curator" who approved removal request and Chinese researcher who submitted it. Whistleblower support activist says names were...

justthenews.com

9

47

104

Steve Massey

@stevenemassey

3 years

Mitochondrial rRNA analysis of the SARS2 contaminated Antarctic datasets identified by Csabai and Solymosi 👇 I ran Metaxa2 on the SARS2 contaminated datasets, then assembled the mit SSU rRNA reads using megahit to produce contigs

Host genomes for the unique SARS-CoV-2 variant leaked into Antarctic soil metagenomic sequencing...

Recently Csabai et al.1 have found a most likely contaminated metagenomic sample set from Antarctica that contained traces of unique SARS-CoV-2 variants. This is a short followup of that report where...

www.researchsquare.com

4

49

101

Steve Massey

@stevenemassey

15 days

Deliberately misleading. The clue to Marburg virus's origin is in its name 'Marburg', a town in Germany. Marburg virus is found in monkeys and monkeys are not native to Germany

Libertarian_Virologist

@ban_epp_gofroc

15 days

Why is @angie_rasmussen still trying to claim that the 1967 Marburg outbreak was caused by zoonosis, rather than a lab leak? It goes to show that many virologists will insist that an outbreak started by lab acquired infections is actually a zoonosis.🤦‍♂️

7

16

90

8

31

105

Steve Massey

@stevenemassey

17 days

22/ To conclude, it appears highly likely that WIV14 was the result of a leak from a scientific facility Its 'frozen' sequence is reminiscent of the Russian H1N1 flu genome, which was also likely released from a scientific facility

Origin of the H1N1 (Russian influenza) pandemic of 1977—A risk assessment using the modified...

In 1977, the Soviet Union (Union of Soviet Socialist Republics [USSR]) notified the World Health Organization (WHO) about an outbreak of H1N1 influenza, which later spread to many countries. The...

onlinelibrary.wiley.com

1

25

106

Steve Massey

@stevenemassey

3 years

An oft-repeated fallacy is that the BANAL viruses are more closely related to SARS2 than RaTG13, now repeated in the article in SciAm by @stuartjdneil @STWorg @past_is_future

4

45

101

Steve Massey

@stevenemassey

2 years

We have discovered 5 more A/B intermediate genomes from Sichuan (with a C/C genotype), in addition to 2 we found previously 🧵 These were not considered by Pekar et al, despite 2 of the 5 conforming to their inclusion criteria 👇

11

36

99

Steve Massey

@stevenemassey

7 days

I have published a paper on my analysis of the RaTG13 dataset RaTG13 is the closest phylogenomic relative of SARS2 Transcriptome and GO enrichment analysis indicates the likely source of the dataset is a bat mating plug, rather than a fecal swab 🧵

Forensic Genomic Analysis Determines That RaTG13 Was Likely Generated from a Bat Mating Plug

RaTG13 is phylogenomically the closest related coronavirus to SARS-CoV-2; consequently, understanding the provenance of this high-value genome sequence is important in understanding the origin of...

www.mdpi.com

7

45

114

Steve Massey

@stevenemassey

1 year

10/ One of the things deeply concerning about this research team is that ZLS's close collaborator Yigang Tong thought that culturing novel PPPs in BSL2 labs and observing if anyone got sick was a good way to assess if the PPP was pathogenic to humans ! h/t @BillyBostickson

5

30

99

Steve Massey

@stevenemassey

1 month

Dr Debarre declines to replicate our analysis reporting the discovery of a genetically manipulated MERS-related infectious clone from Wuhan, and prefers to dismiss it out of hand Probably because she knows our analysis is robust

12

26

101

Steve Massey

@stevenemassey

9 months

Sadly, a lab leak is looking more and more plausible

Biosafety Now!

@BiosafetyNow

9 months

December 18, 2023 Statement On New Revelations About “Project DEFUSE” Documents reported today by U.S. Right to Know reveal that the president of EcoHealth Alliance, Peter Daszak, planned in 2018 to use U.S. Department of Defense funds to perform high-risk virus experiments at

28

232

496

9

16

103

Steve Massey

@stevenemassey

2 years

9/ Bat HKU4r-CoVs are potential pandemic pathogens (PPPs), with the potential to infect human cells 👇 The leak of this material into a rice sequencing dataset indicates serious biosafety violations in Wuhan

Isolation of MERS-related coronavirus from lesser bamboo bats that uses DPP4 and infects human-DP...

Nature Communications - Several human coronaviruses (CoV) have been proposed to emerge from bats but evidence of direct bat-to-human transmission is slim. In this work, the authors isolate a...

www.nature.com

1

19

97

Steve Massey

@stevenemassey

3 years

@R_H_Ebright @HansMahncke They claimed political reasons aka 'international harmony', but this was likely a smokescreen to cover for their real anxiety - that their reckless role in funding dangerous research in Wuhan might be revealed

6

14

89

Steve Massey

@stevenemassey

3 years

I am sure @STWorg will be horrified to discover the error in his SciAm article and will want to correct the record, especially given his academic interest in the spread of 'misinformation'

4

26

93

Steve Massey

@stevenemassey

17 days

4/ The 3 genome sequences were from strains Glenn (PV-3), cold variant P2149 (PV-1) and cold variant P712 (PV-2) Glenn was a cold adapted strain isolated before 1956 from a child in Cincinnati

1

15

97

Steve Massey

@stevenemassey

3 years

Evidence for ‘live’ RaTG13 at WIV Part 1 Over the course of several threads, I will present evidence that the genome of RaTG13, the closest relative of SARS2, was not generated from a fecal swab as claimed but a ‘live’ isolate.

10

37

94

Steve Massey

@stevenemassey

3 months

Unravelling the RaTG13 Dataset's Origin The RaTG13 dataset has overexpressed transcripts which indicate it was mis-sampled from a bat mating plug This provides a reasonable explanation for anomalies in the dataset, described as a 'fecal swab' 🧵

5

35

96

Steve Massey

@stevenemassey

2 years

14/ This study is the product of the superb forensic skills of @humblesci @Daoyu15 @ydeigin @BiophysicsFL @quay_dr

2

7

94

Steve Massey

@stevenemassey

17 days

11/ WIV14 should be more divergent at the sequence level if it had been in circulation for 60 years after splitting from Saukett A The most plausible explanation is that its progenitor was in storage for a substantial amount of time, and then released into the population

1

17

95

Steve Massey

@stevenemassey

11 months

Baric should be next

Select Subcommittee on the Coronavirus Pandemic

@COVIDSelect

11 months

🚨Today is the day🚨 EcoHealth Alliance President @PeterDaszak appears for a transcribed interview. What does Dr. Daszak know about the origins of COVID-19 and dangerous gain-of-function research at the Wuhan Institute of Virology? Americans are ready for answers!

121

343

857

6

17

94

Steve Massey

@stevenemassey

17 days

5/ The P712 cold variant was a serially passaged derivative of strain P712. P712 is a constituent of the oral polio vaccine The P2149 cold variant is likewise a serially passaged derivative of strain P2149 Both cold variants are able to grow at 25C

1

13

91

Steve Massey

@stevenemassey

3 years

Evidence for ‘live’ RaTG13 at WIV Part 3 In two previous threads, I have presented evidence the genome of RaTG13, the closest relative of SARS2, was not generated from a fecal swab as claimed. In this thread I examine whether RaTG13 was generated from a mislabelled oral swab

3

25

91

Steve Massey

@stevenemassey

1 year

Yikes, not sure of the wisdom of this block...

Select Subcommittee on the Coronavirus Pandemic

@COVIDSelect

1 year

🚨Newsflash, @PeterDaszak . Hiding from the truth doesn’t change the facts.

76

322

978

9

19

89

Steve Massey

@stevenemassey

2 years

I wonder if @flodebarre would be so kind as to enlighten us as to where on GISAID she found the raw sequence datasets ?

12

89

Steve Massey

@stevenemassey

2 years

6/ The vector was subsequently used in a paper by Linfa Wang and Zhengli Shi to make a non-infectious SARS clone. ZLS is China’s leading coronavirologist and director of Center for Emerging Infectious Diseases at the Wuhan Institute of Virology (WIV)

Construction of a non-infectious SARS coronavirus replicon for application in drug screening and...

Severe acute respiratory syndrome virus (SARS-CoV) was the causative agent of the SARS outbreaks in 2002-2003. A safer in vitro system is desirable for conducting research on SARS-CoV and to screen...

pubmed.ncbi.nlm.nih.gov

2

14

85

Steve Massey

@stevenemassey

17 days

8/ Saukett A was first obtained from a child, James Sarkett (the name of the strain was misspelled), in California prior to 1954 by medical pioneer Jonas Salk, creator of the IPV Salk never received a Nobel for his work, but perhaps should have done

A lasting link to discovery

DAISYTOWN, Pa. -- The steel braces on Jim Sarkett's legs poke through his blue jeans as he leans back on his living room recliner. He hasn't moved all morning from this corner vantage point --...

archive.triblive.com

1

14

86

Steve Massey

@stevenemassey

1 year

The CGG arginine codon, although being extremely rare in coronaviruses is found in the SARS2 FCS insert It turns out to be a popular choice for synthetic coronavirus sequences

Yuri Deigin

@ydeigin

1 year

@flodebarre @mbw61567742 @Ayjchan It is clear that CGG is loved by many genetic engineers: Moderna has recoded 39 of the 42 SARS2 spike arginines by CGG while Pfizer has recoded 19 of 20 CGx spike arginines by CGG.

7

12

45

5

29

84

Steve Massey

@stevenemassey

6 months

From @COVIDSelect 's letter to Dr Peter Daszak "This comment appears to be materially inconsistent with your testimony assuring the Committees that the proposed work was planned to be done exclusively at UNC" h/t @emilyakopp @USRightToKnow

4

28

84

Steve Massey

@stevenemassey

2 years

10/ Of great concern is the presence of reads corresponding to the MERS receptor binding domain (RBD) spliced into the HKU4r-HZAU-2020 backbone (which we term HKU4r-HZAU-2020+S(MERS). This indicates a second IC is present and represents a GOF chimera of the most lethal kind

3

11

81

Steve Massey

@stevenemassey

2 months

Tabak: "The origin of SARS-CoV-2 has not been identified, despite intensive efforts" Give me a break, NIH has put zero effort in investigating a lab origin, instead preferring to smear investigators

Jimmy Tobias

@JamesCTobias

2 months

New NIH FOIA release re: Tabak, OIG, etc:

3

13

28

2

16

84

Steve Massey

@stevenemassey

2 years

17/ Thanks to my co-authors @humblesci Daoyu @ydeigin @quay_dr for their indefatigability and superb detective work @threadreaderapp unroll

4

7

79

Steve Massey

@stevenemassey

17 days

10/ The high sequence identity, with only 70 nucleotide differences, indicates a recent common ancestor between Saukett A and WIV14 However, Saukett A was isolated < 1954. Given the high mutation rate of polioviruses, the high sequence identity is anomalous

1

14

83

Steve Massey

@stevenemassey

1 year

A new critique of Pekar et al's simulations published by @nizzaneela on PubPeer A bug-fix significantly reduces confidence in the authors' finding of two spillovers...

7

25

80

Steve Massey

@stevenemassey

17 days

9/ The Saukett A genome showed a 99% match to the WIV14 genome, providing a closer match than Glenn This can be seen on the tree below (genetic distance is indicated by branch lengths)

1

13

81

Steve Massey

@stevenemassey

9 months

New paper by @jbloom_lab explores 5 animal CoVs detected in the Huanan Seafood Market In samples collected Jan 12 2020, 4 CoVs are more substantially more abundant than SARS2, and 3 show strongest associations with their putative host animals 🧵

Bloom Lab

@jbloom_lab

9 months

I have performed additional new analyses comparing SARSCoV2 to other animal CoVs in environmental samples from Huanan Market Paper w these new analyses is & full computational pipeline at The new results are summarized below.

5

73

201

4

26

82

Steve Massey

@stevenemassey

17 days

23/ Another example of 'frozen' sequences is that of hand, foot and mouth disease (HMFD) strains sampled in 2007-2009 from China, which bear high identity to the BrCr prototype strain sampled in 1970 USA, indicating a likely lab leak origin

4

19

81

Steve Massey

@stevenemassey

17 days

7/ As Glenn is related to Saukett A (PV-3), which is used to produce inactivated polio vaccine (IPV), the group also sequenced the Saukett A genome They obtained a sample from the British National Institute for Biological Standards and Control (NIBSC)

1

11

81

Steve Massey

@stevenemassey

1 month

More scintillating logic from the 'you're harming science by proposing a hypothesis we don't like' crowd Now predictably morphed into 'you're killing people'

18

15

82

Steve Massey

@stevenemassey

2 years

8/ The sequencing data deposition date of 19 Jan 2020 is significant, as it indicates that this work was done prior to the beginning of the SARS2 pandemic

2

13

78

Steve Massey

@stevenemassey

8 months

A potential explanation for the alanine (A684) in the SARS2 FCS ? Alanine mutational scanning is used to systematically mutate residues in functional sites, to determine if they ablate function, so indicating their importance 🧵

Alanine scanning - Wikipedia

en.wikipedia.org

7

24

81

Steve Massey

@stevenemassey

1 year

A comprehensive dataset of 1535 sarbecoviruses shows a complete lack of an FCS (apart from the one in SARS2)

Rebecc@ ███████

@Rebecca21951651

1 year

1535 SarbeCoVs...& only one w/ FCS? SARS CoV2 @Nature "A comprehensive dataset of animal-associated sarbecoviruses" h/t @quay_dr @WashburneAlex @R_H_Ebright @Ayjchan @zasdak @BiophysicsFL @sciencecohen @jocelynkaiser @johnstravis @martinenserink

13

21

80

7

23

80

Steve Massey

@stevenemassey

1 month

"Débarre’s tactics include exaggerating minor discrepancies, framing routine scientific practices as ethical breaches, and publicly attacking Bloom to undermine his credibility. These actions ... suggest a calculated effort to damage Bloom’s reputation"

Asya d'Nafsha

@asya_nafsha

1 month

8

24

88

6

18

80

Steve Massey

@stevenemassey

17 days

25/ Finally, another intriguing example of a 'frozen' sequence is that of the 2021 Ebola outbreak in Guinea. The genome sequence was little different from 2014 sequences from the same region

3

15

80

Steve Massey

@stevenemassey

2 years

I made a codon usage table of the plasmid spike discovered by @MartinaSisters 🧵 A priori, the sequence can be seen to be strongly optimized, given its extreme codon usage bias

4

22

77

Steve Massey

@stevenemassey

7 months

20/ Are there any journal editors out there brave enough to give our manuscript a fair hearing ?

6

7

77

Steve Massey

@stevenemassey

2 years

7/ Therefore the trail leads to the WIV as source of the novel MERS-related clone

2

11

78

Steve Massey

@stevenemassey

8 months

The WIV have been synthesizing (unreported) infectious clones of genetically modified bat SARSr-CoVs since at least 2017

Holtz

@Biorealism

8 months

@debralex57 @mkeulemans @19961995sw @volkskrant For example, the Wuhan Institute of Virology had a 2017 grant to use infectious cloning to make recombinant SARS coronaviruses. The results of this are unknown. Currently they refuse to share their records with WHO or the NIH.

7

11

43

3

26

80

Steve Massey

@stevenemassey

1 year

Incredible that we still don't know what live isolates the WIV had in their possession In the absence of direct access to WIV researchers, perhaps their close collaborators Eddie Holmes and Ralph Baric might have some relevant documentation

Francisco de Asis

@franciscodeasis

1 year

And live isolate WIV15 is still missing (We already unearthed WIV6 in a non publicly available WIV thesis)

1

7

21

8

16

78

Steve Massey

@stevenemassey

2 years

I have a feeling that in future Kevin’s work will be seen as having been pivotal in alerting the public to the massive dangers of public health by fiat and uber-centralization

Kevin McKernan

@Kevin_McKernan

2 years

Nepetalactone Newsletter: Failure of the linearization reaction in the Pfizer bivalent vaccine manufacturing process

5

41

96

1

17

75

Steve Massey

@stevenemassey

2 years

Another blow against the Huanan market No SARS2r CoVs found in animals, including raccoon dogs, "captured by local traders in rural areas of Wuhan for vendors in animal markets including the Huanan Seafood market"

Bloom Lab

@jbloom_lab

2 years

Yong-Zhen Zhang's new paper analyzing animal samples collected around Wuhan in early 2020 for CoVs is in @VirusEvolution (). Recall his group also reported the first human #SARSCoV2 sequence. (1/3)

5

92

222

6

19

74

Steve Massey

@stevenemassey

10 months

The problem with Eddie Holmes' 'narrative' is that the pangolin data had nothing to say about the artificial looking FCS of SARS-CoV-2

8

13

76

Steve Massey

@stevenemassey

3 years

The microbes present in the SARS2-contaminated Antarctic soil datasets discovered by Csabai/Solymosi can help to better understand the nature of the SARS2 contamination 🧵

Host genomes for the unique SARS-CoV-2 variant leaked into Antarctic soil metagenomic sequencing...

Recently Csabai et al.1 have found a most likely contaminated metagenomic sample set from Antarctica that contained traces of unique SARS-CoV-2 variants. This is a short followup of that report where...

www.researchsquare.com

5

27

72

Steve Massey

@stevenemassey

2 years

7/ The cloning scenario is supported by the presence of the commonly used E.coli pUC57 cloning vector at the 5' end of one of the GX_ZC45r reads This indicates cloning of virus genome fragments, and concomitant amplification - a common step in making infectious clones

2

11

72

Steve Massey

@stevenemassey

17 days

24/ Venezuelan equine encephalitis virus (VEEV) strains isolated in 1995 also have 'frozen' sequences, showing striking genome identities to strains isolated 1962-64 The 1995 strains likely derived from a leaked 1962-64 strain used for vaccine production

4

15

75

Steve Massey

@stevenemassey

2 years

A mysterious addition has been made to Worobey et al and Pekar et al in Science Full of strong statements regarding issues that have not been settled (highlighted) The vibe I am getting from this is 'the beatings will continue until morale improves'

11

16

72