A lot has changed in the world of long-read sequencing. Can you spot the myths from the facts?
• HiFi error rate: 0.1% or 10%?
• Seq cost per Revio SMRT Cell: ~$1,995 or ~$995?
• Output per Revio run: 24 or 360 Gb?
See how you did:
#HiFiFacts
RNA is an essential biomolecule that helps make us who we are.
Which
#RNA
type matches your personality best?
Share your pick in the comments, tag a friend, and follow PacBio for a chance to win a fun prize! T&Cs apply:
#ASHG23
#PacBio
#PacBio
is ready to turn up the music! 🎵 Help us create the soundtrack of the summer for a chance to win a prize. 😎 T&Cs apply:
1. Comment with your song choice
2. Tag a friend
3. Follow PacBio
We're celebrating
#DNADay23
early with a fun giveaway!
The first word you see in the word search puzzle is your science superpower! Leave a comment with the first word you see & tag a friend for a chance to win a prize! T&Cs apply:
PacBio Announces Revio, a Revolutionary New Long Read Sequencing System Designed to Provide 15 Times More HiFi Data and Human Genomes at Scale for Under $1,000
#PacBio
#ASHG22
Join our educational webinar to learn the basics of DNA Sequencing! Find out why long reads are beneficial & get your questions answered by our panel of sequencing gurus:
@lissagoingviral
@OlgaVPettersson
& Michael Hardigan
@UCDavisPlants
Register:
BREAKING: Today, we announce our new collaboration with
@Google
to use machine learning to optimize long-read sequencing data analysis.
#HiFiSequencing
Read more here:
#AGBT24
, you brought the cool factor to the
#PacBio
HiFi Lounge last night. 💯 It was great to see so many friendly and new faces! The fun continues tonight and tomorrow night, including announcing our giveaway winners to the concert of your choice!🥳
#AGBT
DNA Extraction Tip 3: When sequencing plants, it is recommended to obtain the youngest leaf/shoot tissue from an individual plant that has been dark treated (kept out of light) for 24-72 hours.
#Sequencing101
More at:
What exactly is "SMRT Sequencing"? What are "long reads," for that matter? Confused about some of the basics of DNA sequencing, but too afraid to ask? Fear not, friends. We're offering a free Sequencing 101 webinar to break it down for you. Feb 27.
It's here! We’re thrilled to announce the launch of the Sequel II System, reducing project costs & timelines with approximately eight times the data output compared to the previous Sequel System. Look at that beauty! 1/3
We are thrilled to announce a new collaboration with medical genetics leader
@Invitae
to develop an ultra-high-throughput clinical whole genome
#sequencing
platform. See the details:
🎉 Celebrating 1 Year of Revio! 🧬
We are blown away by the exceptional high throughput and outstanding accuracy of the
#Revio
data achieved by our
#PacBio
customers. Here's to many more years of game-changing discoveries!
#ASHG23
#ASHG2023
#RevioAnniversary
We’re spreading the long-read love all this month by giving away free
#Revio
building block kits. Simply RT this post, follow
@pacbio
, and tag 2 friends for a chance to win! T&Cs apply:
#AGBT23
#PacBio
Revio is officially on the way! First commercial shipments are en route to customers worldwide. Join us as we share glimpses into the excitement that's brewing - we can't wait to see what game-changing discoveries are on the horizon!
#Revio
#PacBio
G’day Melbourne! Excited for
#icg2023
this week? Keep an eye out for
#PacBio
trams and billboards scattered throughout the city. Snap a pic & tag us for a chance to win exclusive limited edition swag. Drop by Booth 79-81 for more info!
Meet Revio and Onso- PacBio’s newest long-read and short-read sequencing systems. Differentiated technology with unparalleled accuracy. Your moment of discovery is waiting. Are you ready?
#PacBio
#ASHG22
Checkmate, Chromosome 8: The First End-to-End Sequence of a Human Autosome. Congrats to
@glennis_logsdon
& the
#T2T
team on this amazing work!
#HiFiReads
What an epic way to usher in a new era of
#sequencing
and the announcement of our two new systems, Revio and Onso, with an amazing performance by Maroon 5!
#PacBio
#ASHG22
Super news from
@sangerinstitute
! The UK hub of the
@darwintreelife
& other human & pathogen genetics projects has purchased 7 new Sequel IIe Systems, to add to their 5 current Sequels. They plan to sequence ~2,000 plant & animal genomes in the next 2yrs!
NEWS FLASH: The kit you've been eagerly awaiting is now available! The SMRTbell gDNA Sample Amplification Kit enables whole genome amplification starting from as little as 5 ng of genomic DNA. Find out more about it & our ultra-low DNA input workflow
Back to School! Have you checked out our
#Sequencing101
series? Whether you're a novice or a pro, you're bound to learn something new!
Evolution of DNA Sequencing:
Sequencing steps:
Understanding accuracy:
BREAKING:
@ChildrensMercy
invests in 4 additional Sequel IIe Systems, enabling the top pediatric center to scale up its innovative whole genome research into
#rarediseases
. Its 6 Sequels will be installed in the new nine-story
@ResearchCM
building.
.
Exciting news! DNA Link just stepped up their genomics game by purchasing multiple
#Revio
units, allowing them to deliver even higher quality data and services to their customers. 👏
IT’S OFFICIAL: PacBio Closes Acquisition of Omniome
Today marks the beginning of a new era! Long-read and short-read
#sequencing
platforms together under one roof.
The next step in enabling the promise of genomics to better human health 🧬 Learn more:
🦇 Happy
#BatAppreciationDay
! 🦇
These little balls of fluff are called Honduran bats, also known as Caribbean white tent-making bats, native to the rainforests of Costa Rica.
H/T
@IFLScience
& Milan Zygmunt
BREAKING: PacBio Signs Definitive Agreement to Acquire Omniome!
Our combined forces will challenge the status quo of conventional NGS.
How?
Click here to learn more
We are excited to partner with
@GeneticNijmegen
on
#raredisease
research. Using
#Revio
, we may be able to identify key variants at scale with extraordinary accuracy and make a real difference in providing rare disease answers.
Incredible experience at the
#PacBio
celebration tonight! The lights, the music, and the coming together of the
#ASHG22
community, is there anything better?
A gift for entomologists & geneticists alike! New low-input protocol makes it possible to create high-quality genomes from a single insect. See what
@drsarahdoom
&
@sangerinstitute
achieved together in this
@biorxiv
preprint:
Want to learn about long-read sequencing? Attend our webinar on Feb. 27 to get an intro to our technology & its applications, followed by a panel discussion among experienced users:
@lissagoingviral
,
@OlgaVPettersson
, & Michael Hardigan from
@UCDavisPlants
The largest genome (5 Gb) broke almost every tool commonly used in assembly processes, but they did it... AND created some cool new tools & super science along the way. Huge congrats to the
@genomeark
team on their
#VGPFlagship
release &
@nature
special!
Today, we announce two new high throughput library preparation kits and workflows that will offer never-before-achieved scale for
#Revio
. Learn more about these automated, scalable, and high-performance library preparation solutions:
#PacBio
#AGBT
#AGBT24
NEW: The power of
#PacBio
#HiFisequencing
shines in this
@nature
article highlighting the TRGT method, enabling unprecedented detection and comprehensive discovery of repeat expansions linked to dozens of monogenic diseases and
#cancer
.
Highly accurate, no gaps, no mis-joins — just chromosome 8 in all its glory. The completion of the first-ever fully sequenced human autosome is a remarkable feat and we are honored that PacBio
#HiFiReads
played a pivotal role in helping to achieve it!
Today we announced that our new HiFiViral SARS-CoV-2 solution has successfully sequenced & captured the entire genome of the
#Omicron
variant lineage. Read our press release, including results from
@inqababiotec
, to learn more:
Happy
#DNADay2021
! Want to learn a bit more about DNA sequencing? We've got the series for you:
Evolution of tech:
Sequencing steps:
Ploidy, haplotypes & phasing:
#Sequencing101
News about a new 'complete' human genome was v exciting. What does it mean & how did
#HiFiReads
help the
#T2T
team fill in the final 8% gap? We dive in on our blog:
Insider insight from
@ArangRhie
#SMRTLeiden
talk also available:
The first
#Onso
short-read sequencing system has left the building!
Featuring exceptional SBB chemistry, it unlocks the promise of groundbreaking discovery in
#oncology
, disease research, and beyond. Stay tuned to see what Q40+ accuracy can do.
#PacBio
“We are now embarking on an era where all genetic variation in an individual will be completely discovered. Hundreds, and ultimately thousands, of new human reference genomes will be produced.”
@glennis_logsdon
@mrvollger
& Evan Eichler in
@nresearchnews
#DYK
: >50% of
#epilepsy
can be traced to a genetic cause? When a child presents w/ seizures, genetic testing can help identify >100 underlying, often rare conditions. Which is why we're happy to partner with
@Invitae
to help shorten diagnostic odysseys.
When preparing samples for HiFi sequencing, you not only want DNA free from nicks and degradation, but you also want long fragments (tens of kilobases) to achieve those coveted long reads. Get some top DNA extraction tips on our latest
#Sequencing101
post.
The Consortium for Long Read Sequencing (CoLoRS) is an international collaboration focused on creating a database of frequency information for human variation identified using long-read human whole-genome sequencing.
#PacBio
Tracking the SWEET tooth of TALEs:
@AdamBogdanove
of the
@BogLabCU
has used SMRT Sequencing to uncover host resistance to rice and cotton pathogens. Learn more about his work with TAL effector genes &the creation of a 'TALome'
Researchers have sequenced all the centromeres in a multicellular organism showing that telomere-to-telomere assemblies are now possible! Congrats to
@AmLarracuente
& her colleagues
@UofR
who used SMRT Sequencing for the study published in
@PLOSBiology
NEWSFLASH: Today we're launching new HiFi Sequencing workflow & software updates for the Sequel II + Sequel IIe Systems.
👍 New primers, clean up & binding kits
🙌 New library prep protocol w/ 3x reduction in DNA input
💪 SMRTLink boost
Incredible experience at the
#PacBio
party tonight! The positive energy and the coming together to celebrate the
#ASHG23
community are always one of our favorite parts.
#ASHG2023
Given the state of the pandemic & for the safety of our attendees, staff, colleagues, clients & their communities, we will no longer be hosting/ livestreaming our in-person workshop at
#PAGXXIX
. We look forward to hosting our workshop in a safer environment virtually soon.
Two genomes from one cross-bred cattle, distinguishing maternal & paternal haplotypes? It's possible, thanks to a trio-binning method from
@aphillippy
@sergekoren
@ArangRhie
@tplsmith
. Popular at
#PAGXXVI
, now in preprint & on our blog:
Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome via
@NatureBiotech
. De novo genome assembly produced a contiguous and accurate genome with a contig N50 of >15 Mb & concordance of 99.997%
#HiFiReads
Interested in winning free PacBio sequencing to explore your favorite plant or animal genome? Submit a 90 second video explaining how a high-quality genome will advance your science. Apply for a
#SMRT
Grant by Feb. 22:
We are proud to announce our new European headquarters in London to serve our growing customer demand, as well as the addition of Genesupport as our first commercial service provider in Europe. Learn more here:
#PacBio
Nanopore was worth a shot, right? But now you're ready for true high-accuracy long-read sequencing without compromise, and we're here to help.
Retire your nanopore system and get special pricing on Revio.
See offer:
#PacBio
#Revio
Reveal more for less with groundbreaking savings on
#Revio
system.
Perfect for large studies, Revio captures all types of genomic variation and full-length views of transcripts. Limited time, bring Revio to your team at ~50% the upfront cost.
See how:
E is for evolution! The evolution of the new Sequel IIe System includes increased computational capacity & on-instrument data processing to directly produce highly coveted, highly accurate
#HiFiReads
-- saving users time & money. Learn more:
With the release of the Sequel II System earlier this year, it is now affordable for scientists to access highly accurate long-read sequencing data. DYK we've added enhancements already? Find out more in a webinar w/ product guru Marty Badgett next Wed.
We are humbled that so many customers believe in the power of HiFi. In just 76 days since we announced
#Revio
, we have already received 76 system orders! We can’t wait to see what the
#genomics
community will accomplish with this game-changing platform.
Say goodbye to yesterday’s RNA-Seq — say hello to Kinnex. Explore the cost-effective, scalable RNA solutions provided by the new Kinnex kits.
#PacBio
#ASHG23
#ASHG2023
Just like The Force, our genomes are both powerful and mysterious.
Unleash the full potential of your research with
#Onso
and
#Revio
, and explore the dark regions of the genome.
May the force of genomics be with you!
#MayThe4th
#PacBio
How to improve the accuracy of
#DeepVariant
even further? Tackle phasing. See how the
@GoogleHealth
team improved their popular variant caller to make the most of
#HiFiReads
with a new haplotype channel.
The results are in! Long-read sequencing named 2022 Method of the Year by
@naturemethods
. A win for our customers and the scientific community- let's keep the discoveries coming in 2023!
#Revio
#PacBio
It's finally here!! Our Method of the Year 2022 is....long-read sequencing! 🎉
Read our (free!) Editorial to learn why we felt the time was right to select this class of powerful technologies as the Method of the Year.
Is it possible to generate truly high-quality, long-read data from minuscule amounts of DNA? Absolutely, thanks to our new kit for ultra-low DNA input, which enables whole genome amplification starting from as little as 5 ng of genomic DNA.
Start your year with a win: Bring in long reads, accuracy, uniform coverage, and native methylation calling – Revio system at ~50% less up front with reagent bundles.
See the offer:
🌱 A complete de novo plant genome generation workflow that starts from plant tissue samples & produces high-quality draft genomes w/ relatively modest laboratory & bioinformatic resources... within 7 days? Check out
#LeafGo
by
@kaust_corelabs
🌱
“The ability to find so many variants that were previously missed is exciting, + holds great promise for diagnostic testing in the future.” See what Greg Cooper & colleagues at
@hudsonalpha
found when they applied
#HiFiReads
to unsolved
#raredisease
cases.
Best stuff is in the Supplement! HGSVC says 'As sequencing tech & assembly algorithms continue to evolve, HiFi sequencing has been predicted to predominate because accuracy & higher depth will afford access to even more previously inaccessible regions.'
Extracting DNA for sequencing isn’t always as straightforward as lyse, precipitate, wash, suspend. In this
#Sequencing101
blog, we share tips, tricks, & protocols to help make your DNA isolation easier. Check it out & share widely!
Congratulations to the Uppsala Genome Center (UGC) from
@ngisweden
for winning our recent customer contest with their creative and inspiring name for their Revio system: Rosi!
We love to see the team sporting PacBio colors with their
#Revio
.
Congratulations!
#PacBio
“We are now embarking on an era where all genetic variation in an individual will be completely discovered. Hundreds, and ultimately thousands, of new human reference genomes will be produced.”
@glennis_logsdon
@mrvollger
& Evan Eichler in
@nresearchnews
“SVs are increasingly being recognized as an important class of variants, which need to be considered in evolutionary, population & clinical genomics.” Summary of two structural variation review papers:
@UM_Genetics
@BCM_HGSC
@NatureGenet
@GenomeBiology
Oxford, Liverpool, Edinburgh...and beyond! The UK is now well equipped for
#SMRT
Sequencing, w/ several new Sequel II & IIe Systems installed at three top centres:
@OxfordGenomics
@CGR_UoL
@edgenome
We can't wait to see what discoveries they will enable!