Alejandro Montenegro @aemonten Twitter profile | Pikagi

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Alejandro Montenegro

@aemonten

9,238

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7,390

Following

3,346

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Molecular Biologist, PhD丨Chief Editor CSH Protocols & Head of Integrity in Publishing — @CSHL 丨Scientific Chair @molbiosystems 丨Central Dogma Police丨Chilean 🇨🇱

New York, USA

https://t.co/hjhZgcAGqL

Joined May 2009

Don't wanna be here? Send us removal request.

Pinned Tweet

@aemonten

Alejandro Montenegro

8 months

Thanks to all attendees for making this the best @molbiosystems conference to date. I hope to see you at #mbiosys25 ! Special thanks to all Guest Speakers, including @GreenLabJHMI @AlexandervanOu1 @Blencowe_Lab @deMiguelN @AnaDuhagon @SelFdz @mgschiavon @Rodrigo_G_I (cont...)

@molbiosystems

Molecular Biosystems Conference

8 months

A new edition of @molbiosystems is in the books! #mbiosys23 We thank all speakers and participants for attending the Molecular Biosystems Conference in Puerto Varas, Chile🇨🇱 for exciting discussions on gene regulation. Below are a few highlights.

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Last Seen Profiles

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@aemonten

Alejandro Montenegro

3 years

- No, no, you can only have ONE supplementary figure - Very well, then....

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Alejandro Montenegro

6 months

PhD and MD/PhD

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Alejandro Montenegro

9 months

Waiting for University reimbursement after paying for a conference out of pocket

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@aemonten

Alejandro Montenegro

4 years

Years ago, my PhD advisor had to write an email to IT to ask that the word "f*ck" was not used to block emails, because he was not getting those which included mentions to one of the organisms the lab was studying, "Botryotinia fuckeliana". IT escalated. It was fun.

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Alejandro Montenegro

5 years

Moving a figure in Word

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Alejandro Montenegro

1 year

Who made this?

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Alejandro Montenegro

3 years

When you meet reviewer #2 after your paper was accepted

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Alejandro Montenegro

2 years

Yeah, this is not good.

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Alejandro Montenegro

1 year

I'm shocked

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Alejandro Montenegro

4 years

Right...

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Alejandro Montenegro

1 year

Pedro Pascal as CSH Protocols covers A thread🧵

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Alejandro Montenegro

1 month

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@mbeisen

Michael Eisen

1 month

Science needs dis tracks

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Alejandro Montenegro

2 months

Grants

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Alejandro Montenegro

4 years

Anaphase-looking stache.

@AudacySports

Audacy Sports

4 years

Titans President Steve Underwood's mustache is on another level. 📸 Jim Brown - USA Today Sports

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Alejandro Montenegro

5 months

"Based on these findings, the majority of PhDs would be financially better off if they skipped the postdoc entirely"

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The impact of postdoctoral training on early careers in biomedicine

Nature Biotechnology - While postdocs are necessary for entry into tenure-track jobs, they do not enhance salaries in other job sectors over time.

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@aemonten

Alejandro Montenegro

4 years

Researcher *before* award: "we NEED to submit to Nature" Researcher *after* award: "my advice is, don't worry about CNS or impact factors"

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Alejandro Montenegro

1 year

PIs: "but you're still coming to the lab, right?"

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Alejandro Montenegro

1 year

Me as an editor reviewing world-class science

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@aemonten

Alejandro Montenegro

1 year

When you are doing an RNA extraction and lose the pellet

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@aemonten

Alejandro Montenegro

3 months

"A true mentor is someone who has more imagination about you than you have about yourself"

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What Is a Mentor? | NEJM

Having benefited from excellent mentors over the course of her career, a physician concludes that a true mentor is someone who has more imagination about you than you have about yourself.

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Alejandro Montenegro

1 year

Postdocs talking to their PIs about wanting to leave academia

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@aemonten

Alejandro Montenegro

3 months

"We showed this 25 years ago" Narrator: they, in fact, did not show that.

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@aemonten

Alejandro Montenegro

2 years

Harvard PI showing some data to a Nature editor at a conference

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@aemonten

Alejandro Montenegro

3 years

Reagents are more expensive in developing countries and the grants are smaller. And everything takes longer to ship, too 👍

@SocDevBio

Society for Developmental Biology

3 years

What is a random fact about your research you think everybody should know about? 🤔

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Alejandro Montenegro

1 year

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@aemonten

Alejandro Montenegro

5 years

Just accepted a paper that while it had many shortcomings, the authors transparently and honestly acknowledged and discussed all of the limitations. I like that papers in medicine usually have a "Limitations" section. Maybe papers in the life sciences would benefit from that too

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@aemonten

Alejandro Montenegro

10 months

So, luck

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Early coauthorship with top scientists predicts success in academic careers

Nature Communications - By examining publication records of scientists from four disciplines, the authors show that coauthoring a paper with a top-cited scientist early in one's career predicts...

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@aemonten

Alejandro Montenegro

3 years

Close that tab. You will not read that article. You will never read that article.

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@aemonten

Alejandro Montenegro

4 years

Let me give you a gift.

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@aemonten

Alejandro Montenegro

2 years

🚨BREAKING 🚨 I'm thrilled to announce that I'll be joining @CSHL @CSHLPress as Executive Editor, where I will run CSH Protocols, both the lab manuals & the journal. Incredibly excited about this opportunity to be closer to the community and collaborate with you!

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@aemonten

Alejandro Montenegro

4 months

Ethidium Bromide

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@aemonten

Alejandro Montenegro

3 years

Innovation grants

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@aemonten

Alejandro Montenegro

1 year

"Each slide you show should have a title, and the title should be the conclusion you wish us to draw from the slide" Yes, exactly. Even if some self-proclaimed talk "gurus" hate this.

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We need to talk

Well, that was tragic. I just left a seminar I was very much looking forward to (“to which I was very much looking forward” – thank you Ms. Rosenberg, my sixth-grade grammar teacher). Terrific...

journals.biologists.com

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@aemonten

Alejandro Montenegro

2 years

A new fluorescent RNA tag, developed based on a natural adenine-sensing riboswitch and named Squash, offers superior imaging properties and accurate quantification in living cells.

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Alejandro Montenegro

1 year

New fear unlocked: janitor accidentally destroyed 25 years of scientific research overnight after flipping a switch to stop an "incessant beeping".

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@aemonten

Alejandro Montenegro

3 years

Rejection letter bingo.

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@aemonten

Alejandro Montenegro

5 years

A third of verified gene KO with CRISPR still show residual protein expression owing to translation reinitiation or exon skipping. Several proteins are still functional. The authors call for a systematic analysis of protein levels after genome editing

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Biological plasticity rescues target activity in CRISPR knock outs

Nature Methods - One third of verified gene knock outs with CRISPR still show residual protein expression owing to translation reinitiation or exon skipping. Several proteins are still functional....

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Alejandro Montenegro

1 year

Reviewers can ask for whatever they want; we are asking for their thoughts and opinions. It is the role of the *Editor* to then make a decision on what would be needed for publication, and to disregard requests that are unnecessary or out of scope

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End the wasteful tyranny of reviewer experiments

Nature - Peer review of scientific papers in top journals is bogged down by unnecessary demands for extra lab work, argues Hidde Ploegh.

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@aemonten

Alejandro Montenegro

1 year

When a speaker says "great question" to everyone except you

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@aemonten

Alejandro Montenegro

2 months

Yes.

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@aemonten

Alejandro Montenegro

4 months

Statement from Frontiers: the paper was not only retracted, it was actually *withdrawn*

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Frontiers statement concerning the article "Cellular functions of spermatogonial stem cells in...

Thanks to the crowdsourcing dynamic of open science, we promptly acted upon the community feedback on the AI-generated figures in the article "Cellular function

www.frontiersin.org

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@aemonten

Alejandro Montenegro

8 months

Not every paper requires a mechanism. And it is certainly not a good idea to force authors to explore something just for a relatively weak Fig 5 just to say that the paper "has mechanistic insights"

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@aemonten

Alejandro Montenegro

7 months

"We have created the Penn State Protein Ladder system to produce protein molecular weight markers easily and inexpensively"

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The Penn State Protein Ladder system for inexpensive protein molecular weight markers

Scientific Reports - The Penn State Protein Ladder system for inexpensive protein molecular weight markers

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@aemonten

Alejandro Montenegro

5 years

FISH & ChIPs

@StearnsLab

Tim Stearns

5 years

Beautiful work combining long-read sequencing with ChIP-seq and chromatin fiber FISH to characterize Drosophila centromeres. After many years of work! From @centromellone .

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@aemonten

Alejandro Montenegro

3 years

“It is time for people to understand that Latin American scientists have two last names.”

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@aemonten

Alejandro Montenegro

8 months

Big. Everyone is smart, everyone works hard. Not everyone has the same chances or happens to be at the right place at the right time. Not everyone gets to meet the same people. It plays a big role.

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@aemonten

Alejandro Montenegro

2 years

Mutations we see

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Alejandro Montenegro

7 years

In a hurry? Nucleic acid purification from plants, animals and microbes in under 30 seconds

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@aemonten

Alejandro Montenegro

1 year

You have not used a pipette in years. I think it's fine not to be portraited using one for your PR pictures.

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Alejandro Montenegro

4 years

At Yale. Excellent.

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Alejandro Montenegro

3 years

First day working from home... and I mean *home* home.

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Alejandro Montenegro

3 years

Author contributions

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Alejandro Montenegro

6 years

"Biological replicates don't necessarily have to look exactly the same. This is biology and not a pants factory". Me, today, to a colleague.

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Alejandro Montenegro

3 years

PI in the lab.

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Alejandro Montenegro

1 year

When you are in the audience and your PI shows a slide you put together

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Alejandro Montenegro

3 years

Filing a expense report

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@aemonten

Alejandro Montenegro

6 years

Did you have a paper accepted? A grant? Were you invited to give a talk, or did it go well already? Was your abstract selected? Did an experiment work? Did you solve that bug in the code?Many of us would love to know &congratulate you! We should have a # for all those good things

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Alejandro Montenegro

5 years

Don't write 'mammals' in the title if you mean mice. Don't write 'plants' if you mean A. thaliana. Don't write 'metazoans' if you mean C elegans. C'mon, people.

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Alejandro Montenegro

2 years

Nature paper reporting well known old findings but with new tech.

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Alejandro Montenegro

1 year

I've always wondered how the *other* people in the lab feel when a PI tweets "this is the best/most interesting paper from my lab" or similar when announcing a new publication.

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Alejandro Montenegro

3 months

Sigh...

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Alejandro Montenegro

6 months

NIH advisory group recommends $14,000 boost in postdoc pay. Researchers shouldn’t be in postdoc positions for more than 5 years, the group also suggests.

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NIH advisory group recommends $14,000 boost in postdoc pay

Researchers shouldn’t be in postdoc positions for more than 5 years, the group also suggests

www.science.org

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Alejandro Montenegro

1 year

That DNA! MY EYES!😵

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Alejandro Montenegro

3 years

You really don't 📸

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Alejandro Montenegro

4 months

I checked the abstract of this paper () with an online tool and I got this:

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Alejandro Montenegro

4 years

Did you know that some editors* also look for reviewers on Twitter? _______ *at least one, me.

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Alejandro Montenegro

5 years

I don't have a problem with p-values per se. It's the culture of using arbitrary thresholds for yes/no calls that has to go, together w/ the associated pressure for "significant" results for publishing. Let's embrace uncertainty and the limitations of our studies.

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Alejandro Montenegro

4 years

Can't believe this only got one RT after I posted it a few days ago, so I'm posting it again. Let's stop wasting money on those ads. Great initiative.

@jobRxiv

jobRxiv

4 years

We want to change the way recruitment for scientific jobs is done to save labs and funders money. jobRxiv is free and easy to use If you’re currently advertising #ScienceJobs , please post them on jobRxiv now! #OpenScience

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Alejandro Montenegro

1 year

Oh boy, I need a drink (HT @helenajambor )

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Alejandro Montenegro

3 years

@surlybassey

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Alejandro Montenegro

5 months

What would be the best way to tell a PI that you'd be happy to join their lab as a postdoc, but only if they can pay you rather than relying on you getting a grant? Like, you'd be happy to apply for funding, but want to make sure you'd have a job even if those don't go through

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Alejandro Montenegro

7 months

A new scientific group is being established at IOCB (Prague) by David Sabatini

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A new scientific group is being established at IOCB Prague headed by Dr. David Sabatini

The research group of David M. Sabatini will focus on molecular analysis of growth regulation in animals. This topic complements the chemical and biochemical programs of the Institute.

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@aemonten

Alejandro Montenegro

5 years

@HarmitMalik Found this on FB

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Alejandro Montenegro

3 years

There was never a time in which all non-coding DNA was dismissed as nonfunctional. Ever. So, all of these articles on "hey, wait! it's not junk, you were wrong" are tiresome.

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Alejandro Montenegro

7 years

Any jackass can trash a manuscript

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Any jackass can trash a manuscript, but it takes good scholarship to create one (how MBoC promotes...

www.molbiolcell.org

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Alejandro Montenegro

2 years

Scientific Reports got killed for something similar ("pay for expedited review") years ago

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Updated: Editor quits journal over pay-for-expedited peer-review offer

Authors can pay open-access journal extra to get reviewed in less than 3 weeks

www.science.org

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Alejandro Montenegro

4 months

Perish and publish

@fake_journals

Publishing with Integrity

4 months

"Highly cited scientist published dozens of papers after his death" @RetractionWatch

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Alejandro Montenegro

3 years

@lpachter Dimensionality reduction 😅

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Alejandro Montenegro

2 years

Most accurate part of Don't Look Up: where they distrust the results because they are not from people at Harvard.

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@aemonten

Alejandro Montenegro

3 years

Yeah, same conclusion, but look! The new study has single cell data!

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@aemonten

Alejandro Montenegro

3 years

Nobel Prize winners: Nah, don't worry about Impact Factors. ECRs:

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Alejandro Montenegro

3 years

The Thermo rep visiting the lab

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Alejandro Montenegro

4 months

The figures, my god...

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@aemonten

Alejandro Montenegro

4 months

I checked the abstract of this paper () with an online tool and I got this:

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@aemonten

Alejandro Montenegro

2 years

I edit papers in Powerpoint.

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@aemonten

Alejandro Montenegro

4 years

Are you a new PI in the life sciences? I'm putting together a webinar series & I'm looking for researchers who want to talk about their work! We want to help you reach a wide audience & feature your exciting work. Interested? Sign up below or DM me! Please RT cc @NewPI_Slack

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@aemonten

Alejandro Montenegro

7 years

You don't have to be in academia to be an #actuallivingscientist

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@aemonten

Alejandro Montenegro

7 months

Some people complain that using BioRender makes all papers look the same, but this is the alternative 😅... In any case, if the point made is clear, I don't really mind.

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Alejandro Montenegro

1 year

The audience when someone asks a question in the last talk of the session right before lunch

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Alejandro Montenegro

5 years

Researcher X: "don't use journal titles as a proxy for anything" Also Researcher X: "hey, my paper in Nature is now out!"

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Alejandro Montenegro

3 years

"The image below is representative of 3 biological replicates"

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Alejandro Montenegro

3 years

Tell me you're a sci-fi fan without telling me you're a sci-fi fan....

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Alejandro Montenegro

1 year

"I have published in Nature Cell and Science"

@Tat1anaMoiseeva

Tatiana Moiseeva

@Tat1anaMoiseeva

1 year

How would you like to publish in Nature Cell and Science?🤣 All at the same time! Does it count as three CNS publications at a time? Spherical spam in vacuum, the classic

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Alejandro Montenegro

2 years

Yup, a variation of "stab PCR" See

@0_minyaaa

闵娅 (Min Ya | Minya), PhD

2 years

When I just started twitter I saw someone said that they don’t cut bands in gels and do gel purification then PCR - just use a toothpick to poke the correct band and dip it into PCR directly. With lots of doubt, I tried this out today very casually, and…. THIS MAGIC WORKS. 🤯

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Alejandro Montenegro

3 years

Giving undergraduate students an opportunity to partake in a research project pays back for both students and the lab, says @arunrichardc .

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Alejandro Montenegro

1 year

When I first saw the table of contents, I was gonna say "hey, that thing after the colon is a very bold thing to write in a title". Then I noticed it was written by Bruce Alberts. And I have no problem with it anymore

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The Cell as a Collection of Protein Machines: Preparing the Next Generation of Molecular Biologists

www.sciencedirect.com

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Alejandro Montenegro

5 years

"Euchromatin and heterochromatin have very similar accessibilities, suggesting that transcription factors can penetrate heterochromatin. Thus, DNA accessibility is not likely to be the primary determinant of gene regulation."

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@aemonten

Alejandro Montenegro

10 months

I wonder if people would be interested in a new edition of this, both as contributors and as readers... In addition to the book, all content would be available via our journal, CSH Protocols (), and indexed/discoverable in PubMed

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@aemonten

Alejandro Montenegro

2 years

Maybe someone out there might want to review a manuscript

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@aemonten

Alejandro Montenegro

5 years

It's odd when authors complain about reviewers as if they are a separate group, when reviewers are part of the same pool of researchers and authors themselves. Also, it's quite shocking how standards can change depending on whether you are an author or reviewer. Interesting thing

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@aemonten

Alejandro Montenegro

4 years

"At 74, I in essence removed 9 years from someone else’s career. I should have stepped aside sooner"

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