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Andy Moore Profile
Andy Moore

@aaandmoore

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Cell biologist interested in organelles and how they move. Husband, dad, intermediate filament apologist, and postdoc in the @JLS_Lab at @HHMIJanelia .

Arlington, VA
Joined October 2016
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@aaandmoore
Andy Moore
6 years
For one of the final experiments of my PhD I finally get to image actin in young neurons.
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@aaandmoore
Andy Moore
2 years
Growing microtubule plus-ends (EB3, orange) and endoplasmic reticulum (KDEL, blue) in a cultured cell. 6 min movie acquired at ~ 1 fps. #TubuleTuesday
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@aaandmoore
Andy Moore
4 years
Long day - thought I'd share some actin.
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@aaandmoore
Andy Moore
2 years
Mitochondria (blue) and intermediate filaments (vimentin, orange) in a tissue culture cell. 7ish minute loop. Imaged with airyscan.
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@aaandmoore
Andy Moore
2 years
#Mitosis . Here are a few angles of a dividing HeLa cell. Microtubules are white and the chromosomes are orange. Imaging modality = Spinning disk + deconvolution.
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@aaandmoore
Andy Moore
10 months
In a recent overnight imaging session, I made a startling discovery... COS7 throw midnight dance parties. Who knew? These party animals are dressed in Vimentin-TagGFP (orange) and the ER marker SnapTag-Sec61b (blue) #FluorescenceFriday
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@aaandmoore
Andy Moore
3 years
Cytokinesis. Airyscan movie featuring my favorites: actin = white, mitochondria = red.
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@aaandmoore
Andy Moore
2 years
For this #FluorescenceFriday , I present 1 hour of the endoplasmic reticulum wiggling around:
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@aaandmoore
Andy Moore
2 years
Mitotic rounding. XY (left) and XZ (right) views.
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@aaandmoore
Andy Moore
2 years
Mitosis montage. Orange = chromosomes 🧬, white = microtubules. Not all stages are represented, but left to right shows the general progression from prometaphase to late anaphase. These are max projections of HeLa cells (from deconvolved spinning disk stacks).
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@aaandmoore
Andy Moore
3 years
A 12 hour loop of the endoplasmic reticulum doing its thing.
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@aaandmoore
Andy Moore
1 year
It's like a real-life game of snake 🐍. Check out this #mitochondrion chasing its own tail. Mito label = TMRM. Cell type = COS-7 Scale = 10 µm. Time = mm:ss.
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@aaandmoore
Andy Moore
3 years
Bonus content! I don't think this movie made the final paper, but I still think it's one of my favorite examples of a metaphase mitochondrion pushed around by an actin comet tail. cyan hot = mito orange glow = actin (lifeact) Loop ~40sec. More here:
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@aaandmoore
Andy Moore
4 years
Thank you to @NikonSmallWorld and this years judges for selecting my neuronal actin movie as a winning video! I'm incredibly honored. Check it out and all the other winning videos in the link 👇
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@aaandmoore
Andy Moore
4 years
My lattice light-sheet movie of actin in a dividing cell (acquired at the @AICjanelia ) was named an honorable mention in the 2019 @NikonSmallWorld in motion contest! Hela + Lifact-EGFP. Rendered in @ImarisSoftware
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@aaandmoore
Andy Moore
3 years
Overjoyed/honored/relieved to share our new paper describing multiple actin networks that position and shuffle mitochondria before cell division. @ErikaHolzbaur @CorySimpsonMD @AICjanelia @JLS_Lab Full-text link: Cables at work:
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@aaandmoore
Andy Moore
2 years
Microtubules for #FluorescenceFriday . Depth coded
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@aaandmoore
Andy Moore
2 months
It's not a deep sea dweller. This is actually the endoplasmic reticulum of a cell, segmented and tracked through time.
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@aaandmoore
Andy Moore
3 years
Organelles keeping busy.
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@aaandmoore
Andy Moore
3 years
Here it is with ER (red) and mito (white).
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@aaandmoore
Andy Moore
2 years
Actin (orange) and mitochondria (cyan) in a micropatterned HeLa cell. 🔃
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@aaandmoore
Andy Moore
3 years
Some actin and microtubules looking particularly dynamic in this Cos cell. #cytoskeleton
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@aaandmoore
Andy Moore
2 years
Endoplasmic reticulum in a cell seeded on a micropatterned substrate. Top and side renderings. Cos7 really look like fried eggs 🥚
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@aaandmoore
Andy Moore
3 years
Mitotic mitochondrial actin waves in multiple cell types:
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@aaandmoore
Andy Moore
2 years
Depth-coded ER (StayGold-KDEL) in a Cos7 cell. Almost no bleaching and the ER seemed quite happy. So far this FP is working really well for us!
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@aaandmoore
Andy Moore
2 years
Changing up the spatial and temporal scales for this #FluorescenceFriday . Here is an overnight acquisition of COS-7 cells stably expressing EGFP-Sec61b to label the endoplasmic reticulum. Time = hh:mm:ss.
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@aaandmoore
Andy Moore
2 years
I somehow managed to separate the inner and outer nuclear membranes while doing expansion microscopy. Not sure if this is a bug or a feature🤔
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@aaandmoore
Andy Moore
1 year
Cellular respiration?
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@aaandmoore
Andy Moore
3 years
Like bugs trapped in a spider's web. Mitochondria (blue) and ER (orange) on the airyscan.
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@aaandmoore
Andy Moore
2 years
Intermediate filaments dressed up in my favorite colormap (🔥glow). This is a Cos7 transfected with mApple-tagged vimentin. I gamma adjusted the movie to visualize the lower density filaments moving out towards the periphery.
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@aaandmoore
Andy Moore
3 years
More bonus content that didn't make it into An actin wave bossing around mitochondria. This time without those pesky microtubules (10 uM nocodazole). Maybe I can do a whole "director's cut" on twitter...
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@aaandmoore
Andy Moore
2 years
Slightly higher res closeup
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@aaandmoore
Andy Moore
6 years
Some particularly funky mito dynamics today:
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@aaandmoore
Andy Moore
2 years
Spent the day playing with the AGAVE image viewer from the @AllenInstitute . I'm totally blown away - it's now my go-to for volumes. Here are some fun images of the ER in a Cos7 cell. #FluorescenceFriday
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@aaandmoore
Andy Moore
2 years
Our perspective on endoplasmic reticulum structure has now been published! In this review, we ( @cjobara @JLS_Lab ) examine the diverse nanoscale ER structures that have been described by light (see below) and electron microscopy.
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@aaandmoore
Andy Moore
1 year
GPT4 fixed all of my mediocre imageJ macros. I literally just copy/pasted my code and prompted something like "please make this better" or "please annotate this I don't remember what it does" or "make this work on multichannel images" and it just does it 🤯.
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@aaandmoore
Andy Moore
3 years
The cytoskeleton (or at least some of it). mEmerald-Vimentin, EMTB-mCherry, and Lifeact-TagBFP in a COS-7 cell. #CellBiology
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@aaandmoore
Andy Moore
3 years
Trying to track the gaps between ER tubules. What a mess. This organelle needs to calm down.
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@aaandmoore
Andy Moore
3 years
Spaghetti endoplasmic reticulum can't decide where it wants to go.
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@aaandmoore
Andy Moore
10 months
Here's a curious-looking tissue culture cell imaged every minute for an hour and a half. The cell is labeled with a fluorescent marker for the endoplasmic reticulum. In my professional opinion, it looks like a spooky face.
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@aaandmoore
Andy Moore
4 years
One silver lining of working from home is having the time to browse old data that I had forgotten about. Here are some dynamic actin waves in a zebrafish that I acquired with @DvirGur
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@aaandmoore
Andy Moore
2 years
Invert all the lookup tables
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@aaandmoore
Andy Moore
3 years
Fun with micropatterns. ER in a U2OS cell. ...the nucleus looks so wiggly .
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@aaandmoore
Andy Moore
3 years
OK, here's my last pitch. Mark your calendars for the Cytoskeletal Ensembles Across Scales subgroup on Monday at #cellbio2020 . At 3:04 pm (SG15) I'll be talking about mito-actin interactions as well as these actin comet tails driving bursts of mitochondrial movement.
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@aaandmoore
Andy Moore
3 years
Well that's a nice way to kick off ASCB... my movie of actin dynamics took the top prize in this year's video contest! Thank you judges! If you want to see more actin movies check out the #cellbio2020 subgroup "Cytoskeletal Ensembles Across Scales" Monday, 12/7 from 1:45-4:45 p.
@ASCBiology
American Society for Cell Biology
3 years
Congrats to our Image and Video Contest winners! Check out this video of all the top prize winners! 🔗 And also check out this gallery of ALL entries. So hard to choose! 🔗 Read more here: 🔗
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@aaandmoore
Andy Moore
2 years
Fission! An actin-positive mitochondrion fragments over ~5 minutes ✂️. Lifeact-EGFP (white), Mito-dsRed2 (red). Airyscan movie of an interphase HeLa.
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@aaandmoore
Andy Moore
3 years
GI-SIM of some funky looking mitochondria
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@aaandmoore
Andy Moore
4 years
Vimentin does some pretty interesting things during mitosis (in some cell types). Lattice light-sheet movie. Vim = orange, MTs = blue. #notjustcortical
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@aaandmoore
Andy Moore
3 months
A 10× expanded nucleus stained with Hoechst to label DNA. At this expansion factor you can see gaps in the surface staining. Nuclear pores?
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@aaandmoore
Andy Moore
6 months
Detecting cell boundaries seemed straightforward, until I applied a square root transformation. You'd be amazed at all the stuff lurking in the depths of your histograms.
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@aaandmoore
Andy Moore
3 years
I think I can uncontroversially say that vimentin is the coolest part of the cytoskeleton. We all agree, right? #cytoskeletonwars
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@aaandmoore
Andy Moore
2 years
For #FluorescenceFriday , here is the ER moving at normal speed. The movie was acquired at 31 fps and played back at 31 fps. It really is that dynamic!
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@aaandmoore
Andy Moore
2 years
Vimentin in purple.
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@aaandmoore
Andy Moore
3 years
actin/mito in a developing neuron. Inverted LUTs are always fun.
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@aaandmoore
Andy Moore
2 years
Vimentin in a metaphase RPE1 cell. It seems like mitotic intermediate filaments look different in every cell line that I look at. Check out the cool twisting filaments.
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@aaandmoore
Andy Moore
2 years
A late addition to #FluorescenceFriday . Actin microridges on zebrafish epidermal cells. Labeled with lifeact-GFP.
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@aaandmoore
Andy Moore
3 years
Vimentin does such weird things.
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@aaandmoore
Andy Moore
7 months
This is a ~6h movie of U2OS cells migrating into a scratch. Magenta = labeled vimentin at time 0, green = newly translated vimentin.
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@aaandmoore
Andy Moore
3 years
3D SIM of ER in a Cos7 cell. Color-coded by z position.
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@aaandmoore
Andy Moore
7 years
More mitosis images. I think I have a problem. I'll stop soon, I promise.
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@aaandmoore
Andy Moore
5 years
Highlight of the day was finally seeing these unbelievable actin structures for myself.
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@aaandmoore
Andy Moore
3 years
I've added in the microtubules, but part of me prefers the illusion of mitochondria aimlessly jiggling in a dark void.
@aaandmoore
Andy Moore
3 years
GI-SIM of some funky looking mitochondria
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@aaandmoore
Andy Moore
4 years
A traditional mitochondrial wreath
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@aaandmoore
Andy Moore
2 years
I post a lot of light microscopy movies. I love photons, but I guess electrons are pretty great too. Here's a FIB-SEM stack of a U2OS cell with a 3D mito segmentation.
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@aaandmoore
Andy Moore
4 years
🤢 getting motion sick
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@aaandmoore
Andy Moore
5 months
It seems like you can't post videos on the other place...which is kind of a bummer.
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@aaandmoore
Andy Moore
1 year
A valiant effort. #FluorescenceFriday
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@aaandmoore
Andy Moore
4 months
I'm excited to present some of my recent work on ER-vimentin coupling today at #cellbio2023 . I'll be speaking in the organelle dynamics/quality control joint session in room 254A at 10:05. I can promise lots of live movies (and a bit more info on this 3d rendering)
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@aaandmoore
Andy Moore
3 years
Metaphase mitochondria MIPs
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@aaandmoore
Andy Moore
1 year
For #FluorescenceFriday I tried out PK Mito Orange on some COS-7 cells (dye described here: ). Really impressed. Check out those cristae!
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@aaandmoore
Andy Moore
6 months
Structured illumination movie of vimentin filaments (blue) and ER (orange) for this #FluorescenceFriday . Scale = 2 µm.
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@aaandmoore
Andy Moore
3 years
DNA in mitosis. #mtDNA
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@aaandmoore
Andy Moore
3 years
OK last one for tonight. Colors are depth. I think the LUT is ICA. That mito is moving.
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@aaandmoore
Andy Moore
3 years
Thank you, @NikonSmallWorld ! Honored to have my images recognized. Check out the gallery for all of the beautiful winners this year.
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@aaandmoore
Andy Moore
7 years
I've absolutely fallen in love with cell division red=mito, cyan=microtubules I'm colorblind but I think these colors work together...
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@aaandmoore
Andy Moore
6 months
For reference - in images with high dynamic range (such as this one) it's hard to see very dim signal without saturating brighter pixels. The original image is on the bottom and the square root is on the top.
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@aaandmoore
Andy Moore
5 years
Mito outer membrane tubulation. I don't study this, but I kinda wish I did...
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@aaandmoore
Andy Moore
6 years
Some mitochondria for your morning
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@aaandmoore
Andy Moore
3 years
Oh weird. When I loop this gif back and forth this MEF looks like it's beating.
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@aaandmoore
Andy Moore
5 years
Haven't posted much since I started my postdoc, but imaged some keratin and F-actin earlier today.
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@aaandmoore
Andy Moore
3 years
Nuclear envelope... what are you doing?!
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@aaandmoore
Andy Moore
3 years
Spiky cells #mitosis
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@aaandmoore
Andy Moore
3 years
Mitochondria playing tag on this #FluorescenceFriday
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@aaandmoore
Andy Moore
6 years
Some Actin/Microtubules in a Cos7 cell
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@aaandmoore
Andy Moore
3 months
Actin in a COS-7 cell.
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@aaandmoore
Andy Moore
3 months
Happy #FluorescenceFriday ! Phalloidin/hoechst labeling of COS-7 cells.
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@aaandmoore
Andy Moore
2 years
Mitochondria! Truth be told, I hate tagging the OMM. Seems like the mito are never quite as happy as when I target the FP to the matrix. #MitochondriaWednesday #DefinitelyNotAThing
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@aaandmoore
Andy Moore
1 year
I call this LUT "BarberPole". It's essentially useless unless you are trying to color code an image based on radial position...and even then it's kind of ambiguous. But I made it and I'm proud.
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@aaandmoore
Andy Moore
4 years
Made this stable RPE1 line with F-Tractin-mNG a while back and saw all these strange vesicular structures labeled with the probe. Anybody familiar with this artifact or is this an RPE actin thing? TMRM = orange.
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@aaandmoore
Andy Moore
3 years
Actin tails emanating from a cluster of endosomes 🚀
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@aaandmoore
Andy Moore
2 years
For last #FluoresenceFriday I shared 1 hour of happy endoplasmic reticulum. For this #FluorescenceFriday I present 1 minute of very angry endoplasmic reticulum. Though I guess it really isn't a reticulum anymore 🤔
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@aaandmoore
Andy Moore
3 years
Should you have 45 minutes to spare tomorrow morning, why not come visit my eposter? I'll be in the Mitochondrial Architecture session talking about actin (and mitochondria). #cellbio2020
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@aaandmoore
Andy Moore
6 years
Expansion microscopy. These cells are so big that I'm going to have to start doing some stitching
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@aaandmoore
Andy Moore
1 year
There's something strangely hypnotic about this actin😵‍💫 GI-TIRF-SIM movie of lifeact-GFP at the very bottom of a dividing cell. Acquired at 1fps, played back at 24fps. Perhaps *slightly* overprocessed.
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@aaandmoore
Andy Moore
5 years
I think I've fallen in love with zebrafish over the last couple of days.
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@aaandmoore
Andy Moore
2 years
Depth-coded movie of GalT-GFP in a COS-7 cell. You can't be a full fledged member of the @JLS_Lab without imaging the Golgi at least once 😆.
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@aaandmoore
Andy Moore
2 years
I finally had the chance to try StayGold (). I was very impressed. 11 slice per frame, 300 total frames, almost no visible bleaching. This is an airyscan movie of TdStayGold-Giantin in a Cos7.
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@aaandmoore
Andy Moore
3 years
Happy New Year!! Actin/MTs in a zebrafish scale (acquired with @DvirGur ).
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@aaandmoore
Andy Moore
1 year
I thought these two astrocytes looked pretty interesting. Mitochondria (orange) and DNA (blue) are labeled.
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@aaandmoore
Andy Moore
7 months
Structured Illumination movie of ER and mitochondria for #FluorescenceFriday . This might stretch the definition of movie.
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