Jeremy Garb @GarbJeremy Twitter profile

Pinned Tweet

Jeremy Garb

6 months

The final version of our paper is out now! @NatureMicrobiol If you're interested in how bacteria use NAD+ depletion to their advantage🦠, SIR2 domains, phage mating 🤭, and more :) This paper is for you 🫵 Check out the 🧵 for some details

Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion

Nature Microbiology - The SIR2-domain-containing protein DSR2 from Bacillus subtilis protects against SPR phage infection via NAD+ depletion. Some phages express anti-DSR2 proteins, blocking...

www.nature.com

4

41

137

Last Seen Profiles

@Thabo_Tshaba

@unempyd

@KellerKnoblock

@Tun90029724

@i_army_org

@ugaweeds

@nabeel

@NigelFairs

@CYD2014

@rosyramales

@whoisaffiq

@bryanferry

@belfastairport

@ScottDikkers

@G4lile0

@AllanSsewanyana

@djTORA_jp

@windows_update

@angelasiefer

@RajivRamaswami

@alexiscorbiere

@AFCMace_

@berlinale

@ritualcloak

@WaysideMaine

@burjalarabtour

@annesophiejahn

@BalazsAczel

@THEJamesWhale

@QuollFinance

@Osstev_

@ahhanisha

@SpEdOUSD

@Deltarains

@tubasa_cosplay

@BnbTiger

Jeremy Garb

@GarbJeremy

6 months

We would like to thank @AnnaLopatina5 @AudeBer @MindaugasZare @Curiousphage Azita Leavitt @AdiMillman @GilAmitai and the Siksnys lab and @SorekLab for contributing to this fun paper!

0

4

Jeremy Garb

@GarbJeremy

6 months

Overall we establish NAD+ depletion as a shared mechanism by SIR2 defense systems to protect bacteria against phage! NAD+ seems to be a central molecule in the phage-bacteria interface. Excited to see what comes next :)

1

3

Jeremy Garb

@GarbJeremy

6 months

Finally, we show other systems where SIR2 domains deplete NAD+ and contribute to an abortive infection phenotype, and one system where NAD+ depletion and phage protection does not involve culture collapse.🧐

1

0

1

Jeremy Garb

@GarbJeremy

6 months

How did we find these proteins? We used a “phage mating” technique promoting genetic exchange between phages that are sensitive to DSR2 and phages that are not. Genome analyses of hybrid phages enabled us to pinpoint the exact phage proteins that activate or repress DSR2!😎

1

6

Jeremy Garb

@GarbJeremy

6 months

We show that DSR2 recognizes newly translated phage tail tube proteins to become an active NADase. In addition, we found that some phages in this family carry a small protein, DSAD1, which binds and inactivates DSR2.

1

0

3

Jeremy Garb

@GarbJeremy

6 months

But how is this system activated? How does it know that the cell has been infected?

1

0

2

Jeremy Garb

@GarbJeremy

6 months

Looking more closely at DSR2, we show that it depletes NAD+ in infected cells and stops phage propagation! 🛡️

1

0

Jeremy Garb

@GarbJeremy

6 months

Recently, many anti-phage defense systems were shown to contain SIR2 domains. One of them, described by Gao et al, is DSR2, a single-gene defense system with a SIR2 domain at the N terminus. But how do DSR2 and other SIR2 systems defend against phages? #WhatIsTheMechanism ?

1

0

1

Jeremy Garb

@GarbJeremy

10 months

Congrats @dinahoch ! Amazing work on defence islands🦠🌴🥥

Dina Hochhauser

@dinahoch

10 months

My turn! 🙋🏼‍♀️ Come read my FIRST first-author paper of my PhD @SorekLab , on the hot 🔥 topic of defence islands! 🏝 The defence island repertoire of the Escherichia coli pan-genome

10

41

192

1

0

7

Jeremy Garb

@GarbJeremy

11 months

Check out all these new anti-phage systems hot out the oven! Amazing work @AdiMillman 🦸‍♀️🦠

Adi Millman

@AdiMillman

11 months

OK. I’ve been waiting for this moment for a while… 🤗🫣🥳 Excited to share our new preprint where we discover 21 new anti-phage systems: "An expanding arsenal of immune systems that protect bacteria from phages" @SorekLab 1/7

18

94

388

0

4

Jeremy Garb

@GarbJeremy

11 months

New molecule alert! Happy to be part of this one🦠👨‍🔬

Erez Tzvi Yirmiya

@ErezTzvi

11 months

Super excited to share our new preprint, discovering an anti-Thoeris protein and an immune signaling molecule produced by both plants and bacteria! A super fun and productive collaboration with the @SorekLab and the Kranzusch lab.

6

61

167

0

1

4

Jeremy Garb

@GarbJeremy

1 year

Brilliant stuff by @FrancoisRousset . What a cool way to find defense systems🦠!

François Rousset

@FrancoisRousset

1 year

Thrilled to see the final version of our story now online! 🔥🤩 Phages and their satellites encode hotspots of antiviral systems Main findings below 👇

9

120

358

0

1

5

Jeremy Garb Retweeted

François Rousset

@FrancoisRousset

1 year

Thrilled to see the final version of our story now online! 🔥🤩 Phages and their satellites encode hotspots of antiviral systems Main findings below 👇

Phages and their satellites encode hotspots of antiviral systems

Rousset, Depardieu et al. show that E. coli P2-like phages and their parasitic P4-like satellites carry diverse anti-phage systems. The PARIS system induces abortive infection, triggered by a...

www.cell.com

9

120

358

Jeremy Garb

@GarbJeremy

1 year

We would like to thank @AnnaLopatina5 @AudeBer @MindaugasZare @Curiousphage Azita Leavitt @AdiMillman @GilAmitai and the Siksnys lab and @SorekLab for contributing to this fun paper!

1

0

7